BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0215.0039.p (308 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314018|ref|YP_003778110.1| two component sensor histidine ... 509 e-145 gi|300313666|ref|YP_003777758.1| two component sensor histidine ... 93 2e-20 gi|300311808|ref|YP_003775900.1| two component sensor histidine ... 89 4e-19 gi|300313895|ref|YP_003777987.1| two-component system sensor his... 89 6e-19 gi|300311751|ref|YP_003775843.1| signal transduction histidine k... 88 9e-19 >gi|300314018|ref|YP_003778110.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 492 Score = 509 bits (1311), Expect = e-145, Method: Compositional matrix adjust. Identities = 251/308 (81%), Positives = 280/308 (90%) Query: 1 LSNDLEQQRKMLSFQVIGAIVLAVLLAFWFGLWLSRPLARIELAISRLGENRFDQPIVVK 60 LS LEQQR+ML+ QV+GAIVLAVLLAF FGLWLSRP+AR+E AI RLG+NRFDQPI V+ Sbjct: 185 LSAALEQQRQMLTAQVVGAIVLAVLLAFCFGLWLSRPMARLEQAIGRLGDNRFDQPIDVR 244 Query: 61 GPADLRRLGQQLDWLRQRLADLEAEKSRFLRHVSHELKTPLAALCEGAALLDDEVAGKLT 120 GPAD+RRLGQQLDWLRQRLADLEAEKSRFLRHVSHELKTPLAALCEGAALLDD VAG+LT Sbjct: 245 GPADIRRLGQQLDWLRQRLADLEAEKSRFLRHVSHELKTPLAALCEGAALLDDGVAGQLT 304 Query: 121 DSQREISRILRQNTQALQTQIEDLLRYNEVAFDAQHINRLPVDLRALLYKVIDDQRLQWV 180 DSQREI+RILRQNTQ+LQTQIEDLLRYNEV+FDAQ I+ +PVDLRALL+KVIDDQRLQW+ Sbjct: 305 DSQREIARILRQNTQSLQTQIEDLLRYNEVSFDAQRIHPVPVDLRALLHKVIDDQRLQWL 364 Query: 181 ARDLKVEVEGGARTAVLDPDKFAIVLANLLSNAVRFSPRGGTIRFVLSGHAGRVRLDCID 240 AR+LKVE+EG ART V+DP+K AIVLANLLSNAVRFSP+GG IRF+LS G VR++C+D Sbjct: 365 ARELKVEIEGAARTVVVDPEKMAIVLANLLSNAVRFSPQGGFIRFLLSEAPGLVRVECVD 424 Query: 241 QGPGVAATDAARIFDPFYQGLNQPQGARHGNGIGLSIVREYVLAHNGEVQLVAGPGGAHF 300 QGPGVA TDAARIF+PFYQGLNQP AR GNGIGLS+VREYV AH+G+V LV GGAHF Sbjct: 425 QGPGVAPTDAARIFEPFYQGLNQPTAARRGNGIGLSVVREYVQAHSGKVYLVPREGGAHF 484 Query: 301 RIELPDES 308 RIELPDE Sbjct: 485 RIELPDEK 492 >gi|300313666|ref|YP_003777758.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 479 Score = 93.2 bits (230), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 84/288 (29%), Positives = 130/288 (45%), Gaps = 27/288 (9%) Query: 21 VLAVLLAFWFGLWLS-RPLARIELAISRLGENRFDQPIVVKGPADLRRLGQQLDWLRQRL 79 + AV+L W + L+ +PL + A +LG N P+ GP ++ Q + +++R+ Sbjct: 211 IFAVVLIAWMAVRLAMKPLQVMAEAAEKLGGNIRSAPLATDGPLEVSTAAQAFNAMQRRI 270 Query: 80 ADLEAEKSRFLRHVSHELKTPLAALCEGAALLDDEVAG-KLTDSQREISRILRQNTQALQ 138 D AE++RFL VSH+L++P+ + +L DE K E+ ++ +Q Sbjct: 271 IDNIAERTRFLAAVSHDLRSPITRMKLRTEMLPDERGKEKFRKDLDEMDALISATLNVVQ 330 Query: 139 TQIEDLLRYNEVAFDAQHINRLPVDLRALLYKVIDDQRLQWVARDLKVEVEGGARTAVLD 198 D Q +L +DL +LL + DQ ++ EV A L Sbjct: 331 D------------IDVQERPQL-IDLDSLLESLRTDQ------AEVGGEVTLSGHAAPLQ 371 Query: 199 --PDKFAIVLANLLSNAVRFSPRGGTIRFVLSGHAGRVRLDCIDQGPGVAATDAARIFDP 256 P L NL+ NAVR+ R R L A +V + D GPG+ R+ +P Sbjct: 372 GYPRSLRRCLQNLVENAVRYGQRA---RVQLQDDADQVCIRVDDDGPGIPEALLERVQEP 428 Query: 257 FYQGLNQPQGARHGNGIGLSIVREYVLAHNGEVQLVAGPGGAHFRIEL 304 FY+ G G+GLSI V AH G ++LV PGG R+E+ Sbjct: 429 FYRLERSRNAGTGGFGLGLSIAATVVQAHRGVMKLVNRPGGG-LRVEI 475 >gi|300311808|ref|YP_003775900.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 289 Score = 89.0 bits (219), Expect = 4e-19, Method: Compositional matrix adjust. Identities = 71/229 (31%), Positives = 110/229 (48%), Gaps = 14/229 (6%) Query: 86 KSRFLRHVSHELKTPLAALCEGAA-LLDDEVAGKLTDSQREIS--RILRQNTQALQTQIE 142 K FL +SHEL+TPL A+ LL+D +D+ R + +++ +N + +E Sbjct: 50 KDEFLATLSHELRTPLNAIVGWTQYLLNDH-----SDADRLVKGLQVIERNARLQAQMVE 104 Query: 143 DLLRYNEVAFDAQHINRLPVDLRALLYKVIDDQRLQWVARDLKVEVEGGARTAVL-DPDK 201 DLL + + + P+DL ++ + I R AR +++ G +L DP + Sbjct: 105 DLLDMSRMLAGKLRLETRPMDLASVAEEAIAAVRPAAEARQIELHPALGQGGVILGDPAR 164 Query: 202 FAIVLANLLSNAVRFSPRGGTIRFVLSGHAGRVRLDCIDQGPGVAATDAARIFDPFYQ-- 259 V+ NLL NA++F+PR G + L H V L+ D G G+ +FD F Q Sbjct: 165 LQQVVWNLLMNAIKFTPRQGRVSLSLRQHDDTVALEVSDNGRGIKGEFLGSVFDRFTQED 224 Query: 260 -GLNQPQGARHGNGIGLSIVREYVLAHNGEVQLV-AGPG-GAHFRIELP 305 G G+GL+I R V H G+++ AGPG GA F + LP Sbjct: 225 VSTGSTGSTASGLGLGLAIARHLVELHGGQIRAASAGPGMGAAFTVTLP 273 >gi|300313895|ref|YP_003777987.1| two-component system sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 475 Score = 88.6 bits (218), Expect = 6e-19, Method: Compositional matrix adjust. Identities = 87/307 (28%), Positives = 132/307 (42%), Gaps = 9/307 (2%) Query: 4 DLEQQRKMLSFQVIGAIVLAVLLAFW---FGLWLSRPLARIELAISRLGENRFDQPIVVK 60 DL Q+ + Q+I A+V +L AF L+RPL R+ A RL + + V+ Sbjct: 163 DLRFQQSQFAAQIIIAVVAVMLAAFLVVRISGVLTRPLTRVARATHRLASGDYAIRVEVE 222 Query: 61 GPADLRRLGQQLDWLRQRLADLEAEKSRFLRHVSHELKTPLAALCEGAALLDDEVAGKLT 120 ++ L Q + L L E + F+ VSHEL+TPLA L L+D V Sbjct: 223 SGDEVGMLAQDFNQLAMTLERNEKLRREFMADVSHELRTPLAILNGQLEALEDGV----L 278 Query: 121 DSQREISRILRQNTQALQTQIEDLLRYNEVAFDAQHINRLPVDLRALLYKVIDDQRLQWV 180 + R L+ + L + DL + A R +DL LL ++ Sbjct: 279 QPDAQTLRSLKSEVEHLNKLVGDLYDLSLADAGALTYRRADIDLAPLLRDSAALYSDRFS 338 Query: 181 ARDLKVEVEGGARTAVL-DPDKFAIVLANLLSNAVRFSPRGGTIRFVLSGHAGRVRLDCI 239 R +++ E A V D + +L+NL N++R++ GG ++ L Sbjct: 339 QRGMRLSCELPASMPVHGDAARLQQLLSNLFENSLRYTDAGGRLQVHAHLQQQHWHLVFD 398 Query: 240 DQGPGVAATDAARIFDPFYQGLNQPQGARHGNGIGLSIVREYVLAHNGEVQLVAGP-GGA 298 D PGV AR+F+ F++ A G G+GL+I R V AH G + A P GG Sbjct: 399 DSEPGVPPEVMARLFERFFRVEASRNRASGGAGLGLAICRRIVDAHQGTIGASASPLGGL 458 Query: 299 HFRIELP 305 + LP Sbjct: 459 RVVVVLP 465 >gi|300311751|ref|YP_003775843.1| signal transduction histidine kinase [Herbaspirillum seropedicae SmR1] Length = 446 Score = 87.8 bits (216), Expect = 9e-19, Method: Compositional matrix adjust. Identities = 77/250 (30%), Positives = 122/250 (48%), Gaps = 21/250 (8%) Query: 37 PLARIELAISRLGENRFDQPIVVKG-PADLRRLGQQLDWLRQRLADLEAEKSRFLRHVSH 95 PL + I GE+ QPI + P +++ ++ L R+ A + RF+ +H Sbjct: 179 PLQALRQEIDSRGEDDL-QPIAERLLPREVQAFVAAINGLLARVEAGMAAQRRFVADAAH 237 Query: 96 ELKTPLAALCEGAALLDDEVAGKLTDSQREISRILR-----QNTQALQTQIEDLLRYNEV 150 EL+TPL AL L E G+ S+ R+ R Q + L TQ+ L R Sbjct: 238 ELRTPLTALS-----LQAERLGRAPMSETARERLTRLETGLQRARQLVTQLLALARAQTP 292 Query: 151 AFDAQHINRLPVDLRALLYKVIDDQRLQWVARDLKVEVEGGARTAVL-DPDKFAIVLANL 209 A A P L +L +VI++ + + + ++G VL P + A +L NL Sbjct: 293 AGAALQ----PCSLHQVLREVIEELLPLAQCKQIDLGLDGERDACVLTQPQELAQLLRNL 348 Query: 210 LSNAVRFSPRGGTIRFVLSGHAGRVRLDCIDQGPGVAATDAARIFDPFYQGLNQPQGARH 269 + NA+ ++P GG + + HA V L+ D GPG+AA++ R+F PFY+ P G Sbjct: 349 IDNAIAYTPAGGAVSLRMERHAQWVALEICDTGPGIAASERERVFQPFYR--IDPSG--Q 404 Query: 270 GNGIGLSIVR 279 G+G+GL+IV+ Sbjct: 405 GSGLGLAIVQ 414 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.140 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,030,667 Number of extensions: 45138 Number of successful extensions: 311 Number of sequences better than 1.0e-08: 37 Number of HSP's gapped: 251 Number of HSP's successfully gapped: 37 Length of query: 308 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 223 Effective length of database: 1,217,395 Effective search space: 271479085 Effective search space used: 271479085 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)