BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0215.0029.p (254 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313172|ref|YP_003777264.1| short-chain alcohol dehydrogena... 483 e-138 gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herb... 126 1e-30 gi|300313109|ref|YP_003777201.1| short-chain alcohol dehydrogena... 118 5e-28 gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein... 113 1e-26 gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reduc... 113 1e-26 >gi|300313172|ref|YP_003777264.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 254 Score = 483 bits (1244), Expect = e-138, Method: Compositional matrix adjust. Identities = 237/254 (93%), Positives = 245/254 (96%) Query: 1 MSQSSGRLAGKTVLITAAAQGIGRASVELFAREGARVIATDISKQHLDELAGIANVETHL 60 MS S+GRLAGKTVLITAAAQGIGRAS ELFAREGARVIATDISK HLDELAGIA VETHL Sbjct: 1 MSASTGRLAGKTVLITAAAQGIGRASTELFAREGARVIATDISKPHLDELAGIAGVETHL 60 Query: 61 LDVTDDAAIKTLVAKIGAVDVLFNCAGYVAAGNILDCDDKAWDFSFNLNAKAMFHTIRAV 120 LDVTDDAAIK LVAKIG +DVLFNCAGYVAAGNIL+CDDKAWDFSFNLNAKAMFHTIRAV Sbjct: 61 LDVTDDAAIKALVAKIGTIDVLFNCAGYVAAGNILECDDKAWDFSFNLNAKAMFHTIRAV 120 Query: 121 LPGMLAKKSGSIVNIASAASSVKGVANRFAYGASKAAVVGLTKSVAADFVAQGVRCNAIC 180 LPGMLAKK+GSIVNIASAASSVKGVANRFAYGASKAAVVGLTKSVAADFVAQG+RCNAIC Sbjct: 121 LPGMLAKKAGSIVNIASAASSVKGVANRFAYGASKAAVVGLTKSVAADFVAQGIRCNAIC 180 Query: 181 PGTIESPSLNQRITTQAKETGKSEAEVRQAFVGRQPMGRIGKAEEVAALALYLASEESAF 240 PGTIESPSLNQRI+TQAKETGKSE EVR AFVGRQPMGRIGKAEEVAALALYLAS+ES F Sbjct: 181 PGTIESPSLNQRISTQAKETGKSEEEVRAAFVGRQPMGRIGKAEEVAALALYLASDESNF 240 Query: 241 TTGAIHMIDGGWSN 254 TTG+IHMIDGGWSN Sbjct: 241 TTGSIHMIDGGWSN 254 >gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 126 bits (317), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 87/256 (33%), Positives = 132/256 (51%), Gaps = 23/256 (8%) Query: 8 LAGKTVLITAAAQGIGRASVELFAREGARVIATDISKQHLDELAGIANVETHLLDV---- 63 L GK LIT A+ GIGRA+ LFAR GA +I T + L+++A + + V Sbjct: 4 LQGKVALITGASAGIGRATALLFARHGAALILTARRQDALEQVAALICAQGGRASVVAGD 63 Query: 64 -----TDDAAIKTLVAKIGAVDVLFNCAGYVAAGNIL-DCDDKAWDFSFNLNAKAMFHTI 117 T + + + + G +D+ N AG V A L + + W + + N + F Sbjct: 64 VGEAATHERGVAMALDEFGGLDIAINNAGAVGACKPLAEISPQQWQDTLHANLTSAFLGA 123 Query: 118 RAVLPGMLAKKSGSIVNIASAASSVKGVANRFAYGASKAAVVGLTKSVAADFVAQGVRCN 177 R +P MLA+ +G+IV +S S G+ AYGA+KA ++GL K +AAD+ QG+R N Sbjct: 124 RCQIPAMLARGAGAIVFTSSFVGSSAGLPGMAAYGAAKAGLMGLVKGIAADYAVQGIRAN 183 Query: 178 AICPGTIESPSLNQRITTQAKETGKSEAEVRQAFVGRQPMGRIGKAEEVAALALYLASEE 237 A+ PG +++ + G + + +Q G M RI + EE+AA AL+LAS Sbjct: 184 ALLPGGVDT------------DMG-GDPQQKQWAAGLHAMKRIAQPEEIAAAALFLASPM 230 Query: 238 SAFTTGAIHMIDGGWS 253 ++F TG DGG S Sbjct: 231 ASFVTGTALYADGGNS 246 >gi|300313109|ref|YP_003777201.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 266 Score = 118 bits (295), Expect = 5e-28, Method: Compositional matrix adjust. Identities = 81/260 (31%), Positives = 138/260 (53%), Gaps = 17/260 (6%) Query: 8 LAGKTVLITAAAQ-----GIGRASVELFAREGARVIATDISKQHLDELAGIANVETHLL- 61 + GK +++T A G G+A+ L+AREG RV+A D S + + I E + Sbjct: 5 MQGKAIIVTGAGSVGEGWGNGKAAAVLYAREGGRVLAVDRSIEAALQTQDIIRSEGGVCE 64 Query: 62 ----DVTDDAAIKTL----VAKIGAVDVLFNCAGYVAAGNILDCDDKAWDFSFNLNAKAM 113 DV+ + +K + + G VDVL N G G ++ +++W+ +N ++ Sbjct: 65 VLAADVSRNTEVKAIAEAAMDHFGRVDVLHNNVGIADVGGPVETSEESWNRLVAVNQTSL 124 Query: 114 FHTIRAVLPGMLAKKSGSIVNIASAASSVKGVANRFAYGASKAAVVGLTKSVAADFVAQG 173 F T + VLP M ++ G+IVNI+S A+ Y A+KAA++ TK+VA + G Sbjct: 125 FLTHKHVLPIMEKQRKGAIVNISSLAALRWIGFPYLGYTATKAAILAFTKNVAMQYAPMG 184 Query: 174 VRCNAICPGTIESPSLNQRITTQAKETGKSEAEVRQAFVGRQPMGRIGKAEEVAALALYL 233 +R N + PG +++P + + + +A G E ++R + PMG +G A +VA +L+L Sbjct: 185 IRANCVLPGMMDTPMIREPL--KASYGGDIE-QMRAKRNAQCPMGFMGDAWDVAHASLFL 241 Query: 234 ASEESAFTTGAIHMIDGGWS 253 AS+ + + TG ++DGG S Sbjct: 242 ASDHARYITGLDMIVDGGLS 261 >gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein] reductase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 113 bits (283), Expect = 1e-26, Method: Compositional matrix adjust. Identities = 84/264 (31%), Positives = 137/264 (51%), Gaps = 39/264 (14%) Query: 8 LAGKTVLITAAA--QGIGRASVELFAREGARVIATDISK------------QHLDELAGI 53 L K +IT A G+G ++ L A +GAR++ D+ + QHL +A Sbjct: 3 LKDKVAIITGGAGQNGLGFSTARLMAAQGARIVILDLERAEPAGAAARLGAQHLGLVA-- 60 Query: 54 ANVETHLLDVTDDAAIKT----LVAKIGAVDVLFNCAGYVAAGNILDCDDKAWDFSFNLN 109 DVTD AA + ++ + G +D+L N AG +L+ K +D +++ Sbjct: 61 --------DVTDKAACEAAAAAVLKQFGRIDILVNNAGVTQPAKLLEITGKDYDRILDVS 112 Query: 110 AKAMFHTIRAVLPGMLAKKSGSIVNIAS-AASSVKGVANRFAYGASKAAVVGLTKSVAAD 168 + + +AVLP M A++SGSIV I+S +A G+ Y A+KA V+GL +++A + Sbjct: 113 LRGTLYMSQAVLPAMQAQQSGSIVCISSVSAQRGGGILGGPHYSAAKAGVLGLARAMARE 172 Query: 169 FVAQGVRCNAICPGTIESPSLNQRITTQAKETGKSEAEVRQAFVGRQPMGRIGKAEEVAA 228 F QG+R N I PG I S +N+ I + K +AE+ Q P+GR+G ++V Sbjct: 173 FGPQGIRVNCITPGLI-STDINKDILSAEK-----KAEIAQTI----PLGRLGGPDDVGG 222 Query: 229 LALYLASEESAFTTGAIHMIDGGW 252 + ++LAS+ S + TG ++GG Sbjct: 223 VCVFLASDLSQYCTGVTLDVNGGM 246 >gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 261 Score = 113 bits (283), Expect = 1e-26, Method: Compositional matrix adjust. Identities = 88/255 (34%), Positives = 123/255 (48%), Gaps = 24/255 (9%) Query: 7 RLAGKTVLITAAAQGIGRASVELFAREGARVIATDISKQHLDELAGIANVE---THLLDV 63 R GK V++T AA GIG A+ F+ EGARV+ D L ++ E DV Sbjct: 11 RFEGKVVIVTGAASGIGEATARRFSDEGARVLLADRDAAALGKVFDSLPPERTAARETDV 70 Query: 64 TDDAAIKTLV----AKIGAVDVLFNCAGYVAAGNILDCDDKAWDFSFNLNAKAMFHTIRA 119 + ++ LV + G +DVL + AG A GN+ + + W N +F+ R Sbjct: 71 SHHEQVRQLVDFAIERFGQLDVLVSDAGVFAEGNVTEVSPEDWHRVQATNVNGVFYGARE 130 Query: 120 VLPGMLAKKSGSIVNIASAASSVKGVA---NRFAYGASKAAVVGLTKSVAADFVAQGVRC 176 LP L K G IVN+AS V G+A N AY ASK AV LT+++A DF +GVR Sbjct: 131 ALP-HLEKTRGCIVNVAS----VSGLAADWNLSAYNASKGAVCNLTRAMALDFGRKGVRI 185 Query: 177 NAICPGTIESPSLNQRITTQAKETGKSEAEVRQAFVGRQPMGRIGKAEEVAALALYLASE 236 NA+C PSL T + + F R +GR E+AA+ +LAS Sbjct: 186 NAVC------PSLTHTAMTADM---ADDPPLLDKFAERIALGRGADPLEIAAVITFLASP 236 Query: 237 ESAFTTGAIHMIDGG 251 +++F G +DGG Sbjct: 237 DASFVNGVNLPVDGG 251 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.129 0.362 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 659,451 Number of extensions: 23185 Number of successful extensions: 247 Number of sequences better than 1.0e-08: 48 Number of HSP's gapped: 163 Number of HSP's successfully gapped: 48 Length of query: 254 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 171 Effective length of database: 1,226,865 Effective search space: 209793915 Effective search space used: 209793915 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)