BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0215.0007.p (134 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313538|ref|YP_003777630.1| hypothetical protein Hsero_4253... 162 6e-42 >gi|300313538|ref|YP_003777630.1| hypothetical protein Hsero_4253 [Herbaspirillum seropedicae SmR1] Length = 181 Score = 162 bits (411), Expect = 6e-42, Method: Compositional matrix adjust. Identities = 73/132 (55%), Positives = 92/132 (69%) Query: 3 NLVKNWDNKAQPVLCALIRSQADWDYWFQPATTMRSQRALTPSVEYFETRQLLLATRVIQ 62 VKNWD++ QPV C LIRS+A+WD W QPA TM +R PS ++FE +Q LL R + Sbjct: 50 GFVKNWDDQRQPVFCTLIRSRAEWDEWMQPAPTMGDRRPTGPSSDHFERKQYLLVARTMT 109 Query: 63 APDAAERSQIFAADKVTMRNGVLKLEYRFVKPVSGATYQVKDFLLVEIPKDRYTTGPVEF 122 APDAAER +F V +R+G L+L Y + P SGA+Y++KD+L+V IPKDRYTTGPV F Sbjct: 110 APDAAERPHVFTPLSVRLRDGTLRLNYLYRAPASGASYRIKDYLMVSIPKDRYTTGPVVF 169 Query: 123 IENGSKVCRIAR 134 EN KVC I R Sbjct: 170 YENDRKVCEINR 181 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.134 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 385,938 Number of extensions: 13464 Number of successful extensions: 22 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 22 Number of HSP's successfully gapped: 1 Length of query: 134 Length of database: 1,619,870 Length adjustment: 76 Effective length of query: 58 Effective length of database: 1,260,010 Effective search space: 73080580 Effective search space used: 73080580 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 125 (52.8 bits)