BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0214.0023.p (85 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313572|ref|YP_003777664.1| 5,10-methylenetetrahydrofolate ... 155 5e-40 >gi|300313572|ref|YP_003777664.1| 5,10-methylenetetrahydrofolate reductase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 282 Score = 155 bits (392), Expect = 5e-40, Method: Compositional matrix adjust. Identities = 70/84 (83%), Positives = 77/84 (91%) Query: 1 VPGIMPITNYTQLMRFSDMCGTEIPRWIRLKLASYGDDADSIRAFGLDVVTRLCEQLLEG 60 + GIMPITNY+QLMRFSDMCGTEIPRWIRLKLASYGDD +SIRAFGLDVVT+LCE+LL G Sbjct: 198 IAGIMPITNYSQLMRFSDMCGTEIPRWIRLKLASYGDDTESIRAFGLDVVTQLCERLLAG 257 Query: 61 GAPGLHFYTLNQPAATMAIWQRLV 84 GAPGLHFY+LNQ T AIWQ+LV Sbjct: 258 GAPGLHFYSLNQAGPTQAIWQQLV 281 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.141 0.449 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 288,735 Number of extensions: 9913 Number of successful extensions: 35 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 35 Number of HSP's successfully gapped: 1 Length of query: 85 Length of database: 1,619,870 Length adjustment: 55 Effective length of query: 30 Effective length of database: 1,359,445 Effective search space: 40783350 Effective search space used: 40783350 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 123 (52.0 bits)