BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0214.0016.p (229 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313568|ref|YP_003777660.1| glutathione S-transferase [Herb... 152 2e-38 gi|300313567|ref|YP_003777659.1| tRNA nucleotidyltransferase [He... 110 1e-25 gi|300309973|ref|YP_003774065.1| glutathione S-transferase [Herb... 65 5e-12 >gi|300313568|ref|YP_003777660.1| glutathione S-transferase [Herbaspirillum seropedicae SmR1] Length = 233 Score = 152 bits (384), Expect = 2e-38, Method: Compositional matrix adjust. Identities = 73/121 (60%), Positives = 88/121 (72%), Gaps = 5/121 (4%) Query: 76 LEKAGKNAITLVIGNKNYSSWSMRPWVVLTAFDIPFREIRICLDQPDTASKIAEYSAAGR 135 L+KAG++ TLVI NKNYSSWSMRPWV + AFDIPF+E+R+ LDQ DTA++IA Y+ +GR Sbjct: 14 LQKAGRHLPTLVIANKNYSSWSMRPWVAMVAFDIPFKEVRLLLDQADTAAEIARYTGSGR 73 Query: 136 VPVLLDGQTHIWDSLAICEYLAEQFPDRALWPQETAAXFPDRALWPQERARAQHHGRNAF 195 VPVLLDG IWDSL+ICEYLAEQFPDR LWP T A R++ + H G Sbjct: 74 VPVLLDGDIAIWDSLSICEYLAEQFPDRHLWPARTDARAIARSICAE-----MHSGFTGL 128 Query: 196 R 196 R Sbjct: 129 R 129 >gi|300313567|ref|YP_003777659.1| tRNA nucleotidyltransferase [Herbaspirillum seropedicae SmR1] Length = 410 Score = 110 bits (275), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 53/63 (84%), Positives = 56/63 (88%) Query: 1 MKIYVVGGAVRDELLGLPVQDHDYVVVGATPEDMLAQGFRPVGKDFPVFLHPDTQEEYAL 60 MK Y VGGAVRD LLGLPV+D D+VVVGATPE +LA GFRPVGKDFPVFLHP TQEEYAL Sbjct: 1 MKTYTVGGAVRDGLLGLPVKDRDHVVVGATPEQLLALGFRPVGKDFPVFLHPHTQEEYAL 60 Query: 61 ART 63 ART Sbjct: 61 ART 63 >gi|300309973|ref|YP_003774065.1| glutathione S-transferase [Herbaspirillum seropedicae SmR1] Length = 218 Score = 65.1 bits (157), Expect = 5e-12, Method: Compositional matrix adjust. Identities = 43/122 (35%), Positives = 60/122 (49%), Gaps = 5/122 (4%) Query: 85 TLVIGNKNYSSWSMRPWVVLTAFDIPFREIRICLDQPDTASKIAEYSAAGRVPVLLDGQT 144 TL I +KNYSSWS+R W++ + F E + D P ++I S++ VP L G Sbjct: 5 TLRITSKNYSSWSLRGWLMARFAGLSFEEDIVPPDDPSARAEILLLSSSILVPSLHHGDV 64 Query: 145 HIWDSLAICEYLAEQFPDRALWPQETAAXFPDRALWPQERARAQHHGRNAFRLHRPAQRH 204 +WD+LAI EYL E P L P + AA R++ + H G +A R P Sbjct: 65 LVWDTLAIAEYLNEIRPAAKLLPADLAARAHCRSVCGE-----MHSGFSALRAALPMNLK 119 Query: 205 EH 206 H Sbjct: 120 GH 121 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.137 0.436 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 861,606 Number of extensions: 37769 Number of successful extensions: 110 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 111 Number of HSP's successfully gapped: 3 Length of query: 229 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 147 Effective length of database: 1,231,600 Effective search space: 181045200 Effective search space used: 181045200 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 129 (54.3 bits)