BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0214.0014.p
         (407 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300313567|ref|YP_003777659.1| tRNA nucleotidyltransferase [He...   639   0.0  
gi|300309964|ref|YP_003774056.1| tRNA nucleotidyltransferase/pol...    70   4e-13

>gi|300313567|ref|YP_003777659.1| tRNA nucleotidyltransferase
           [Herbaspirillum seropedicae SmR1]
          Length = 410

 Score =  639 bits (1648), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 309/404 (76%), Positives = 344/404 (85%), Gaps = 1/404 (0%)

Query: 1   MKIYVVGGAVRDELLGLPVQDHDYVVVGATPEDMLAQGFRPVGKDFPVFLHPDTQEEYAL 60
           MK Y VGGAVRD LLGLPV+D D+VVVGATPE +LA GFRPVGKDFPVFLHP TQEEYAL
Sbjct: 1   MKTYTVGGAVRDGLLGLPVKDRDHVVVGATPEQLLALGFRPVGKDFPVFLHPHTQEEYAL 60

Query: 61  ARTERKTAPGYKGFVFHTDSAVTLEQDLARRDLTINAIAKGEDGVLVDPYDGRGDIGRRV 120
           ARTERKTAPGYKGF FH    VTLE DLARRDLTINAIA+ EDG LVDPY GR DI  RV
Sbjct: 61  ARTERKTAPGYKGFAFHAAPDVTLEDDLARRDLTINAIAQDEDGTLVDPYHGRADIAARV 120

Query: 121 FRHVSDAFIEDPVRILRLARFAARFPDFTVAPETNALMQKMVAAGEVDALVPERVWQELA 180
           FRHVSDAFIEDPVRILR+ARFAARF DFTVAPETNALMQ+MV  GEVDALVPERVWQEL+
Sbjct: 121 FRHVSDAFIEDPVRILRVARFAARFADFTVAPETNALMQRMVEQGEVDALVPERVWQELS 180

Query: 181 RGMMEQTPSRMFNVLRECGALARILPELDALWGVPQPARYHPEVDTGVHVMMVLDYAAGR 240
           RG+MEQ PSRMF VLR CGAL RILPELDALWGVPQP ++HPE+DTGVHVM+V+D+AA +
Sbjct: 181 RGLMEQKPSRMFEVLRACGALQRILPELDALWGVPQPEKWHPEIDTGVHVMLVVDWAAAQ 240

Query: 241 SYSLGVRFAALMHDLGKGTTPPELWPSHHGHEGRSVELVQGICDRLKVPNDCRDLAVITA 300
            ++L +RFAALMHDLGKGTTPPE+WP HHGHE RS  L + +C RLKVP +CRDLA++TA
Sbjct: 241 GFTLPIRFAALMHDLGKGTTPPEMWPRHHGHEARSERLAEQVCARLKVPGECRDLALMTA 300

Query: 301 REHGNVGRAFELRPATIVNLLARCDGFRKPERFHDMLQASECDRRGRTGFEDVAFPQLDY 360
           REHGNVGRAFE+RP T+V   +RCD FRKP+RF DML+A+ECD RGRTG E   FPQ  Y
Sbjct: 301 REHGNVGRAFEMRPDTLVKFFSRCDAFRKPQRFIDMLRAAECDHRGRTGLEQQPFPQAAY 360

Query: 361 LMGALKAAQGVDAGAIAARYREQPQRIPEAILAARTEAVAEYAA 404
           L GAL+AAQ +DAGAI A+ + QPQRIPEAI AARTE VA + A
Sbjct: 361 LEGALQAAQQIDAGAI-AQAQSQPQRIPEAIQAARTEQVAAFVA 403


>gi|300309964|ref|YP_003774056.1| tRNA
           nucleotidyltransferase/poly(A) polymerase
           [Herbaspirillum seropedicae SmR1]
          Length = 534

 Score = 69.7 bits (169), Expect = 4e-13,   Method: Compositional matrix adjust.
 Identities = 66/195 (33%), Positives = 103/195 (52%), Gaps = 18/195 (9%)

Query: 2   KIYVVGGAVRDELLGLPVQDHDYVVVGATPEDMLAQGFRP---VGKDFPVFLHPDTQEEY 58
           K ++VGGAVRD LLG+  +D D V   ATPE  + Q FR    +G+ F + +H    +E 
Sbjct: 75  KAFIVGGAVRDLLLGIKPKDFD-VATNATPE-QVKQLFRRAFIIGRRFQI-VHVMFGQEL 131

Query: 59  ALARTER----KTAPGYK-GFVFHTDSAVTLEQDLARRDLTINAIAKG-EDGVLVDPYDG 112
               T R    + AP  + G V   ++     +D  RRD TINA+        ++D + G
Sbjct: 132 IEVTTFRGASAEAAPKDEHGRVLRDNTFGEQHEDATRRDFTINAMYYDPASQAVLDYHGG 191

Query: 113 RGDIGRRVFRHVSDA---FIEDPVRILRLARFAARFPDFTVAPETNALMQKMVAAGEVDA 169
             DI  +  R + +    + EDPVR+LR+ RFAA+   FT+ P ++A ++ M  A  +D 
Sbjct: 192 IADIRDKKLRIIGEPEARYREDPVRMLRVVRFAAKL-KFTIDPASSAPIRVM--APLIDN 248

Query: 170 LVPERVWQELARGMM 184
           +   RV+ E+ + +M
Sbjct: 249 VPAARVFDEMLKLLM 263


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.323    0.139    0.419 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,313,978
Number of extensions: 57620
Number of successful extensions: 153
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 151
Number of HSP's successfully gapped: 2
Length of query: 407
Length of database: 1,619,870
Length adjustment: 87
Effective length of query: 320
Effective length of database: 1,207,925
Effective search space: 386536000
Effective search space used: 386536000
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 132 (55.5 bits)