BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0214.0006.p (224 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313562|ref|YP_003777654.1| polypeptide chain release facto... 198 3e-52 >gi|300313562|ref|YP_003777654.1| polypeptide chain release factors methylase [Herbaspirillum seropedicae SmR1] Length = 277 Score = 198 bits (503), Expect = 3e-52, Method: Compositional matrix adjust. Identities = 113/157 (71%), Positives = 126/157 (80%) Query: 9 AGASLGDALKNAPLDTLENRILLGHALQLSRIQLITQSERLLTDDEAVRLTALYRRRLQG 68 AG +L LK APLD LENRILL HAL+L+R+QLITQSER L+ EA L AL RRL+G Sbjct: 8 AGCTLATVLKTAPLDPLENRILLCHALRLTRVQLITQSERQLSAAEAETLAALLARRLRG 67 Query: 69 EPIAYIVGEREFFGLAMAVTPDVLIPRPDTELLVELALERLPQQGKALDMGTGSGAIAVA 128 EPIAYIVG+REF+GL + V+PDVLIPRPDTELLVELALERLPQ G ALDMGTGSGAIAVA Sbjct: 68 EPIAYIVGQREFYGLDLRVSPDVLIPRPDTELLVELALERLPQGGSALDMGTGSGAIAVA 127 Query: 129 IAHTRPDADMTALDVSEGALKVAASNAQRHGAKVRLF 165 IAHTRPDA +TALD S AL +A NA H +VRL Sbjct: 128 IAHTRPDAQVTALDASPAALAIARENASTHQVRVRLL 164 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.136 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 686,457 Number of extensions: 27394 Number of successful extensions: 132 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 132 Number of HSP's successfully gapped: 1 Length of query: 224 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 142 Effective length of database: 1,231,600 Effective search space: 174887200 Effective search space used: 174887200 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)