BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0212.0006.p (358 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313918|ref|YP_003778010.1| transmembrane protein [Herbaspi... 290 2e-79 gi|300313329|ref|YP_003777421.1| transmembrane protein [Herbaspi... 277 9e-76 gi|300313782|ref|YP_003777874.1| transmembrane protein [Herbaspi... 220 1e-58 gi|300312843|ref|YP_003776935.1| response regulator containing a... 74 1e-14 gi|300310865|ref|YP_003774957.1| response regulator containing a... 70 3e-13 >gi|300313918|ref|YP_003778010.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 515 Score = 290 bits (741), Expect = 2e-79, Method: Compositional matrix adjust. Identities = 148/307 (48%), Positives = 199/307 (64%), Gaps = 9/307 (2%) Query: 6 MVFGLFGWMLYTSHREASQYAVETSQNITLMAQRDIVRNIEILSLSLDALANRYQSPM-F 64 MV + LY S+++ Q AV + N+TL++QR I RN+E+LSLSLD LA R+Q P+ Sbjct: 39 MVMAISASTLYQSYQQTRQSAVSNASNVTLVSQRAISRNLEMLSLSLDTLAYRFQHPLSA 98 Query: 65 RQLTPNQQYSYLFGGVEAAKHVVVMAVLNTVGGVVVSSL--PGTPANTYGERIYFTIQRD 122 R+L Q+Y+YLFG H+ VMAVL+ G V VSS G A Y +R YF RD Sbjct: 99 RRLNDAQRYAYLFGSTTNVSHIAVMAVLDADGRVQVSSRLGRGNGAPHYADRAYFIAHRD 158 Query: 123 QPETGLYISPPINAKFDRDLQVVVLSKRLSNRDGSFAGIVVMALDLAYFRELFSGIMLGE 182 P GLY+S P+ A +LQVVVLSKRLSN DGSFAG+VVMALDLAYFRELF G+ LG+ Sbjct: 159 SPTVGLYVSRPMQASLGNELQVVVLSKRLSNEDGSFAGVVVMALDLAYFRELFEGLALGD 218 Query: 183 DGVMSLYSRSGVVYMRVPYSAAMIGRDISTTAXSLSLSLSLKIKSLIQQNSGSFFAQAVN 242 +G++SLYS G+ YMRVPY +IGRDIS T ++++++ + GSFFA A N Sbjct: 219 EGIISLYSDEGIAYMRVPYRETIIGRDISQTGN------FQRLRAMLSEEEGSFFAHATN 272 Query: 243 DGIKRLYAFRNVPGTQLVVFAGRSEKHIFRHWTENLYTLGFMMLFFMTVCVALFHILRNE 302 DG++RLY FR +PG+ L+VF G +E+ IF+ W + Y + + F + L +R E Sbjct: 273 DGVRRLYTFRRIPGSPLIVFVGYAEEAIFKTWRKTFYAVLVSLAIFGLLTAWLLLHVRQE 332 Query: 303 LQKRKNA 309 L++R A Sbjct: 333 LRRRVTA 339 >gi|300313329|ref|YP_003777421.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 501 Score = 277 bits (709), Expect = 9e-76, Method: Compositional matrix adjust. Identities = 140/313 (44%), Positives = 201/313 (64%), Gaps = 12/313 (3%) Query: 2 LLMAMVFGLFGWMLYTSHREASQYAVETSQNITLMAQRDIVRNIEILSLSLDALANRYQS 61 L++ MV + G LY +R+ A+ + N+TL+ QR I RN+EILSL++D LA RYQ Sbjct: 14 LIIMMVLSICGASLYEGYRQTRISAITEANNVTLIVQRAISRNMEILSLAMDTLAYRYQH 73 Query: 62 PM-FRQLTPNQQYSYLFGGVEAAKHVVVMAVLNTVGGVVVSSL----PGTPANTYGERIY 116 P+ + ++Y+YLFG AA H+ +A+L+ G V V+S P N Y ER Y Sbjct: 74 PLDLTHASDEERYNYLFGSTAAAHHITALAILDADGYVRVTSRRSASPSVVVN-YSERTY 132 Query: 117 FTIQRDQPETGLYISPPINAKFDRDLQVVVLSKRLSNRDGSFAGIVVMALDLAYFRELFS 176 FT+ RD + GL+IS PI A +L VVVLSKRLS +DGSF G+V+MALD++YFR+LF+ Sbjct: 133 FTVHRDSADAGLFISRPIKANLGNELPVVVLSKRLSRKDGSFDGVVLMALDMSYFRDLFT 192 Query: 177 GIMLGEDGVMSLYSRSGVVYMRVPYSAAMIGRDISTTAXSLSLSLSLKIKSLIQQNSGSF 236 G+ LGEDG+MSLYS GV YMRVPY + IGR+I+ ++++++ N G+F Sbjct: 193 GLTLGEDGIMSLYSDDGVTYMRVPYHPSSIGREITNNPN------FQRLRAMLVDNDGNF 246 Query: 237 FAQAVNDGIKRLYAFRNVPGTQLVVFAGRSEKHIFRHWTENLYTLGFMMLFFMTVCVALF 296 FA+A DG++RLY FR +P L+V GRSE+ IF+ W + LY++ +++ F + L Sbjct: 247 FARAHTDGVERLYTFRRIPDAPLIVLLGRSERFIFKTWYDTLYSVLLLLIIFTALAGVLL 306 Query: 297 HILRNELQKRKNA 309 ++R EL KR A Sbjct: 307 MLVRRELLKRVAA 319 >gi|300313782|ref|YP_003777874.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 475 Score = 220 bits (561), Expect = 1e-58, Method: Compositional matrix adjust. Identities = 128/303 (42%), Positives = 178/303 (58%), Gaps = 21/303 (6%) Query: 14 MLYTSHREASQYAVETSQNITLMAQRDIVRNIEILSLSLDALANRYQSPMFRQLTPNQQY 73 +LY SHR+ + A+E NI +AQR I RN+E+L+L+LD+L RY+ ++ Q+ Sbjct: 9 VLYQSHRQVERAAIEHGANIARIAQRSISRNVELLTLALDSLTWRYRHTQLHEMPLQQKM 68 Query: 74 SYLFGGVEAAKHVVVMAVLNTVGGVVVSS------LPGTPANTYGERIYFTIQRD---QP 124 +L G A++V + +++T G +VV S LPG Y + +FT + Q Sbjct: 69 EFLLGENIEARYVAALGIVDTDGELVVGSAALSQILPGP----YAQSDFFTAHQTHHAQG 124 Query: 125 ETGLYISPPINAKFDRDLQVVVLSKRLSNRDGSFAGIVVMALDLAYFRELFSGIMLGEDG 184 +TGLYIS P+ + +R +QVVVLSKRL+ DGSFAGI +M L L YFR+LF G+ L DG Sbjct: 125 DTGLYISAPLTVRLERRMQVVVLSKRLAQPDGSFAGISLMVLYLDYFRDLFQGLTLENDG 184 Query: 185 VMSLYSRSGVVYMRVPYSAAMIGRDISTTAXSLSLSLSL-KIKSLIQQNSGSFFAQAVND 243 V+SLYS G+ YMR+PY+ A IG L++S L +I S GS+FA+A D Sbjct: 185 VISLYSADGLAYMRIPYNEAFIG-------SRLNISEDLERIFSPSGPAQGSYFARAQQD 237 Query: 244 GIKRLYAFRNVPGTQLVVFAGRSEKHIFRHWTENLYTLGFMMLFFMTVCVALFHILRNEL 303 G + LY F VPGT VF GR+ IFR W LY + F+M F + L +R EL Sbjct: 238 GPRWLYTFARVPGTGWTVFIGRTGARIFREWYNMLYAVLFLMASFCIASIYLIRRVRVEL 297 Query: 304 QKR 306 Q+R Sbjct: 298 QRR 300 >gi|300312843|ref|YP_003776935.1| response regulator containing a CheY-like receiver and a GGDEF domains protein [Herbaspirillum seropedicae SmR1] Length = 525 Score = 74.3 bits (181), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 46/191 (24%), Positives = 90/191 (47%), Gaps = 10/191 (5%) Query: 101 SSLPGTPANTYGERIYFTIQRDQPETGLYISPPINAKFDRDLQVVVLSKRLSNRDGSFAG 160 S+ P PAN +R YF RD + G ++ + ++ ++ +S+RL++ DGSFAG Sbjct: 127 SAPPLAPANN-SDRDYFIWHRDHDDVGPHVGKALLSR-STGRWIIPMSRRLNHPDGSFAG 184 Query: 161 IVVMALDLAYFRELFSGIMLGEDGVMSLYSRSGVVYMRVPYSAAMIGRDISTTAXSLSLS 220 +++ LD+ +F +L+ + +G+ G ++L R G + R+P+ I RD S Sbjct: 185 VLLATLDINHFNQLYRTVEIGKGGSIALLLRDGTLLTRMPFDPGYINRDFSQG------- 237 Query: 221 LSLKIKSLIQQNSGSFFAQAVNDGIKRLYAFRNVPGTQLVVFAGRSEKHIFRHWTENLYT 280 L ++ L G + D +R +++ V LV+ + + W + + Sbjct: 238 -RLFLEGLPHSPFGYLEVVSPLDARQRFLSYQAVDKYPLVLVVTLAREEALADWKQQTFV 296 Query: 281 LGFMMLFFMTV 291 G +L + + Sbjct: 297 YGAAVLLLLAI 307 >gi|300310865|ref|YP_003774957.1| response regulator containing a CheY-like receiver domain and a GGDEF domain transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 554 Score = 70.1 bits (170), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 52/226 (23%), Positives = 103/226 (45%), Gaps = 19/226 (8%) Query: 73 YSYLFGGVEAAKHVVVMAVLNTVGGVVVSSL-----PGTPANTYGERIYFTIQRDQPETG 127 + ++ V + + + V++ G V SS G+P YF + + Sbjct: 103 HDFMIRSVASLPQLAQLTVVDEQGRTVASSQQRMVQTGSPVQDD----YFQYHQHRRSDR 158 Query: 128 LYISPPINAKFDRDLQVVVLSKRLSNRDGSFAGIVVMALDLAYFRELFSGIMLGEDGVMS 187 +I P+ + + + +S+R+++ DGSFAG++V +DLAYF+ + +G GV+ Sbjct: 159 PFIGQPMRSHVT-GQRTLTVSRRINHVDGSFAGVIVAEIDLAYFQRYYEQFNIGPHGVIL 217 Query: 188 LYSRSGVVYMRVPYSAAMIGRDISTTAXSLSLSLSLKIKSLIQQNSGSFFAQAVNDGIKR 247 G + +R P A M R S ++S +L+ K + Q GSF ++ DG++R Sbjct: 218 FGLTGGSLILRRPLLADMTDR-------SPAMSEALR-KHMEQARRGSFAMRSERDGVER 269 Query: 248 LYAFRNVPGTQLVVFAGRSEKHIFRHWTENLYTLGF-MMLFFMTVC 292 + F+ + ++ I W+ +++ G ++L +C Sbjct: 270 ISCFQQGEAYPIFTVVALAKDDILEDWSRHVWVRGIGVVLLLAVIC 315 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.324 0.137 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 984,828 Number of extensions: 37464 Number of successful extensions: 166 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 156 Number of HSP's successfully gapped: 6 Length of query: 358 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 272 Effective length of database: 1,212,660 Effective search space: 329843520 Effective search space used: 329843520 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 131 (55.1 bits)