BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0212.0003.p (400 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309878|ref|YP_003773970.1| 2-polyprenyl-6-methoxyphenol hy... 99 6e-22 gi|300313232|ref|YP_003777324.1| 3-(3-hydroxyphenyl) propionate ... 91 2e-19 gi|300310882|ref|YP_003774974.1| 2-polyprenyl-6-methoxyphenol hy... 84 2e-17 >gi|300309878|ref|YP_003773970.1| 2-polyprenyl-6-methoxyphenol hydroxylase [Herbaspirillum seropedicae SmR1] Length = 589 Score = 99.0 bits (245), Expect = 6e-22, Method: Compositional matrix adjust. Identities = 105/365 (28%), Positives = 158/365 (43%), Gaps = 61/365 (16%) Query: 19 VIAGAGPVGLFLSCELRLAGLSVLVLEQAANPHSPLKQLPFGIRGLSIPTVEAFHRRGLL 78 VI GAGPVGL + +L G++V++++ + + + F R L I F R G Sbjct: 34 VIIGAGPVGLACAIDLARQGIAVVLVDDDDTLSTGSRAICFAKRTLDI-----FDRIGCG 88 Query: 79 DAIKAPPLAVNAAASTPHWMQQQRRAGGHFAGMQFHLDRIDRTKWPYHLPGGPPPPPA-- 136 + + N +++ Q A +F L LP PA Sbjct: 89 QRMVDKGVGWNRGKV---FLRDQ-------AVYEFDL-----------LPESGHARPAFI 127 Query: 137 -RGRQHIESVLAARAHAMG-VEIMRGLGVDGFQQSDEH----VDIQAGGQTYRARWLLGC 190 + ++E L +A +EI V ++H VD G T +AR+++ Sbjct: 128 NLQQYYVEGYLLEQAQQWPHIEIRWKNKVSAIAHHEDHEALTVDTPQGSYTLKARYVVAA 187 Query: 191 DGGRSTVRRIGGFAFTGTEPEFTGYSVEIEMADPQLLAPGRHHTAAGMYTFQPP------ 244 DG RS +R++ G E + + +AD ++ AP A + F PP Sbjct: 188 DGSRSPLRKM-----MGLESQGHTFRDRFLIADVKMDAP---FPAERWFWFDPPFHPQQS 239 Query: 245 -------GTVAMVDFDGGAFHRTEAVTREHVESVLRRVS---GIDVSVTSLRLAATWTDR 294 V +DF G + V + E V+ RV G +VS SL + +T Sbjct: 240 VLLHRQPDDVWRIDFQLG--WDADPVLEKTPERVIPRVRALLGEEVSF-SLEWVSVYTFS 296 Query: 295 AFQAVAYRQGRVLLAGDAAHVHSPLGGQGLNLGLGDAMNLGWKLAATIRGEAPEGLLDSY 354 + +R GRVL GDAAH SP G +G N G+ DA NL WKLA ++G+AP+ LLDSY Sbjct: 297 CVRMERFRHGRVLFVGDAAHCVSPFGARGANSGIQDAENLAWKLAHVLQGKAPDSLLDSY 356 Query: 355 HAERH 359 +ER Sbjct: 357 ASERE 361 >gi|300313232|ref|YP_003777324.1| 3-(3-hydroxyphenyl) propionate hydroxylase [Herbaspirillum seropedicae SmR1] Length = 544 Score = 90.9 bits (224), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 112/374 (29%), Positives = 158/374 (42%), Gaps = 57/374 (15%) Query: 7 PSASSAPSTYDAVIAGAGPVGLFLSCELRLAGLSVLVLEQAANPHSPLKQLPFGIRGLSI 66 P+ + +I GAGPVG+ L L G+S L++E+ + + P + G+ Sbjct: 8 PAVPAQVMQAQVLIVGAGPVGVTLGNLLGTYGISTLLIERNLD----VLDYPRAV-GIDD 62 Query: 67 PTVEAFHRRGLLDAIKAPPLAVNAAASTPHWMQQ-QRRAGGHFAGMQFHLDRIDRTKWPY 125 + F G+ DAI + + P M RR R + Sbjct: 63 EALRTFQSAGMADAI-----VRDVIQNVPMRMYTADRRCFAEIQPTTREFGWYRRNLFSQ 117 Query: 126 HLPGGPPPPPARGRQHIESVLAARAHAMGVEIMRGLGVD-GFQQS--DEHVDIQAGGQ-T 181 L R +H+ + GVE++ + + G Q S DEH G Q T Sbjct: 118 PLGEATLRLGLRRFEHV-------SLRSGVELLDLVQDEAGVQASIIDEH-----GAQAT 165 Query: 182 YRARWLLGCDGGRSTVR-RIGGFAFTGTEPEFTGYSVEIEMADPQLLAPGRHHTAAGMYT 240 RA++++GCDGGRSTVR +I + G +E + DP L AP +TA Sbjct: 166 LRAQYVVGCDGGRSTVREKILKLPYEGKTHPAKWVVIECD-GDP-LDAP---YTALHCEP 220 Query: 241 FQP------PGTVAMVDF------DGGAF---HRTEAVTREHVESVLRRVSGIDVSVTSL 285 +P P V +F DG + + REHV DV ++ Sbjct: 221 QRPYVCLALPYGVRRWEFMLFPGEDGEQMLAPEKVRELLREHVR---------DVDALNI 271 Query: 286 RLAATWTDRAFQAVAYRQGRVLLAGDAAHVHSPLGGQGLNLGLGDAMNLGWKLAATIRGE 345 A +T + A + GR+ LAGDAAH+ P GQGLN GL DA NL WKLA ++G Sbjct: 272 IRARVYTHNSRVAGSLVCGRICLAGDAAHITPPWIGQGLNAGLRDAFNLAWKLAWVLQGR 331 Query: 346 APEGLLDSYHAERH 359 LL SYH ERH Sbjct: 332 MKPELLASYHEERH 345 >gi|300310882|ref|YP_003774974.1| 2-polyprenyl-6-methoxyphenol hydroxylase [Herbaspirillum seropedicae SmR1] Length = 547 Score = 84.3 bits (207), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 95/365 (26%), Positives = 146/365 (40%), Gaps = 51/365 (13%) Query: 13 PSTYDAVIAGAGPVGLFLSCELRLAGLSVLVLEQAANPHSPLKQLPFGIRGLSIPTVEAF 72 PS + V+ GAGP+GL + E GL V+VL+ + + + R L I + Sbjct: 23 PSRHPVVVIGAGPIGLTAALECAARGLPVVVLDDNDTVSIGSRAVCYAKRPLEI-----W 77 Query: 73 HRRGLLDAIKAPPLAVNAAASTPHWMQQQRRAGGHFAGMQFHLDRIDRTKWPYHLPGGPP 132 R G+ AP + + + + R F + H ++ P Sbjct: 78 DRLGV-----APRMVEKGVSWKLGKVFFEEREVYRFDLLPEHAHKM-------------P 119 Query: 133 PPPARGRQHIESVLAARAHAMGVEIMRGL-GVDGFQQSDEH--VDIQAGGQTYR--ARWL 187 + ++E L A + +R + Q D H +D++ +R A W+ Sbjct: 120 AMINLQQYYLEQYLVEACQAQPLIDLRWKHKMISLHQDDTHATLDVETPDGIFRMEADWV 179 Query: 188 LGCDGGRSTVRRIGGFAFTGTEPEFTGYSVEIEMADPQLLAPGRHHTAAGMYTFQPP--- 244 + CDG S R++ G F+G +F + +AD + A A + F PP Sbjct: 180 IACDGANSDTRKMVGAEFSG---QF--FQDRFLIADVIMKA---DFPAERWFWFDPPFHR 231 Query: 245 ----------GTVAMVDFDGGAFHRTEAVTR-EHVESVLRRVSGIDVSVTSLRLAATWTD 293 V +DF G E + E V ++ + G +V L + + Sbjct: 232 GQSVLLHKQCDNVWRIDFQLGWEADPEEEKKPEKVIPRIQAMLGKEVEF-ELEWVSVYQF 290 Query: 294 RAFQAVAYRQGRVLLAGDAAHVHSPLGGQGLNLGLGDAMNLGWKLAATIRGEAPEGLLDS 353 + R RVL AGDAAH SP G +G N G+ D NL WKL I G+AP +DS Sbjct: 291 ACRRIDKMRYQRVLFAGDAAHQVSPFGARGANTGVQDIDNLVWKLKLVIDGKAPPSFIDS 350 Query: 354 YHAER 358 YH ER Sbjct: 351 YHEER 355 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.137 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,290,267 Number of extensions: 55891 Number of successful extensions: 229 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 228 Number of HSP's successfully gapped: 3 Length of query: 400 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 313 Effective length of database: 1,207,925 Effective search space: 378080525 Effective search space used: 378080525 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)