BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0211.0008.p (214 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312232|ref|YP_003776324.1| signal transduction protein [He... 329 1e-91 gi|300311938|ref|YP_003776030.1| hypothetical protein Hsero_2626... 187 5e-49 gi|300311595|ref|YP_003775687.1| two component response regulato... 64 1e-11 gi|300310564|ref|YP_003774656.1| regulator of the two-component ... 61 9e-11 >gi|300312232|ref|YP_003776324.1| signal transduction protein [Herbaspirillum seropedicae SmR1] Length = 274 Score = 329 bits (844), Expect = 1e-91, Method: Compositional matrix adjust. Identities = 159/188 (84%), Positives = 171/188 (90%) Query: 1 MNLEALFQQQAALPSIPKVVQEVIESFNNDNVSIEDIAKKLSADQVLSAKLLRLANSSYY 60 MNLEALFQQQ ALPSIPKVVQEVIESFNN++VSIE+IAKKL+ADQVLSAK+LRLANSSYY Sbjct: 1 MNLEALFQQQTALPSIPKVVQEVIESFNNESVSIEEIAKKLAADQVLSAKILRLANSSYY 60 Query: 61 HVSRSIGTVDDAVLMLGFMTVRTLVVSTGLTGSFKSMSGVDLKLFWRYSLHTAIVAKWLA 120 HVSR+IGTVDDAVLMLGFMTVRTLVVSTG+ GSFK + GVDLKLFWRYSL TA+VAKWLA Sbjct: 61 HVSRTIGTVDDAVLMLGFMTVRTLVVSTGMAGSFKKLPGVDLKLFWRYSLDTALVAKWLA 120 Query: 121 KKQGLNSDLAFTVGLMHAIGQLVMHAAMPEQTRQVDKIASTIDARRLDVEHTSFGYDFAD 180 K+ LNSD AFTVGLMHAIGQLVMHA MPEQ QVDK+A +DARRLDVE SFGYDFAD Sbjct: 121 KRGKLNSDFAFTVGLMHAIGQLVMHAGMPEQALQVDKVAGPLDARRLDVERKSFGYDFAD 180 Query: 181 VGAGLANR 188 VGA LA R Sbjct: 181 VGAELARR 188 >gi|300311938|ref|YP_003776030.1| hypothetical protein Hsero_2626 [Herbaspirillum seropedicae SmR1] Length = 290 Score = 187 bits (475), Expect = 5e-49, Method: Compositional matrix adjust. Identities = 87/190 (45%), Positives = 134/190 (70%), Gaps = 4/190 (2%) Query: 1 MNLEALFQQQAALPSIPKVVQEVIESFNNDNVSIEDIAKKLSADQVLSAKLLRLANSSYY 60 M L++L Q LP++PKVVQ+++ SFN +++S +IA++++ D LSAK+LRLANS+++ Sbjct: 1 MELKSLVDQPGKLPTVPKVVQQLMASFNAEDISAHEIAQQIATDPALSAKVLRLANSAFF 60 Query: 61 HVSRSIGTVDDAVLMLGFMTVRTLVVSTGLTGSFKSMSGVDLKLFWRYSLHTAIVAKWLA 120 H SR+IGTVDDA+ +LGF+ VR LV+ G+ +FK+ G+DL FWR++L+TA+ A+WLA Sbjct: 61 HASRTIGTVDDALRILGFIMVRNLVLGNGMVAAFKNTRGMDLHQFWRHTLYTAVAARWLA 120 Query: 121 KKQGL----NSDLAFTVGLMHAIGQLVMHAAMPEQTRQVDKIASTIDARRLDVEHTSFGY 176 G+ N D FTVG+MH IGQL +HA +D+ ++ +D+ R + E ++G+ Sbjct: 121 MHAGMVGEDNGDAVFTVGMMHGIGQLQLHAVAANAVAPLDRHSNVLDSDRAEREKEAWGF 180 Query: 177 DFADVGAGLA 186 +ADV A LA Sbjct: 181 HYADVSAELA 190 >gi|300311595|ref|YP_003775687.1| two component response regulator protein [Herbaspirillum seropedicae SmR1] Length = 310 Score = 63.5 bits (153), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 46/183 (25%), Positives = 91/183 (49%), Gaps = 5/183 (2%) Query: 7 FQQQAALPSIPKVVQEVIESFNNDNVSIEDIAKKLSADQVLSAKLLRLANSSYYHVSRSI 66 Q LP+ V ++++ +++++ ++ ++ D VL+ +L+++AN + R + Sbjct: 13 MQDSEVLPAPSGVRLKIMQMCQQEDIALPELVAQVQGDPVLAGRLIQIANIPNINKGRMV 72 Query: 67 GTVD-DAVLMLGFMTVRTLVVSTGLTGSFK--SMSGVDLKLFWRYSLHTAIVAKWLAKK- 122 +V+ + +LM+G VR + ++ LT S + + D FW S A + L Sbjct: 73 ASVNVELLLMVGLHAVRQMALAISLTESARRGDCAEFDYDAFWSRSTAMACAGQALGDLL 132 Query: 123 QGLNSDLAFTVGLMHAIGQLVMHAAMPEQTRQVDKIASTIDARRLDVEHTSFGYDFADVG 182 Q FT+GL+ IGQL + +A P++ ++ +A DA+ E SFG+D + Sbjct: 133 QVAPPAEMFTIGLLAGIGQLGLASARPKRYGEL-LLAGAKDAQLRVQERESFGFDHLALA 191 Query: 183 AGL 185 A + Sbjct: 192 AAM 194 >gi|300310564|ref|YP_003774656.1| regulator of the two-component system protein [Herbaspirillum seropedicae SmR1] Length = 317 Score = 60.8 bits (146), Expect = 9e-11, Method: Compositional matrix adjust. Identities = 52/184 (28%), Positives = 84/184 (45%), Gaps = 17/184 (9%) Query: 12 ALPSIPKVVQEVIESFNNDNVSIEDIAKKLSADQVLSAKLLRLANSSYYHVSRSIGTVD- 70 ALPS V ++ DN + ++ + AD LS +++R+ N R I +V Sbjct: 18 ALPSPGGVRLTIMRLCAQDNPDMAELIHLVQADPALSGRIIRIVNVLNAQRVRPIASVSM 77 Query: 71 DAVLMLGFMTVRTLVVSTGL--TGSFKSMSGVDLKLFWRYSLHTAIVAKWLAKKQGLNSD 128 D++L++G VR L ++ L T + S + L FW +S A A Q + S Sbjct: 78 DSLLLVGLQAVRQLALALSLAETRAPASCANFSLPAFWAHSFAMACSA------QAVTSQ 131 Query: 129 L-------AFTVGLMHAIGQLVMHAAMPEQTRQVDKIASTIDARRLDVEHTSFGYDFADV 181 L FT GL+ IG+L + +A P + A ARR +E + FG++ ++ Sbjct: 132 LRVAPLGEMFTTGLLANIGKLTLASAQPTGYSALLTRALDAPARR-AMEQSLFGFNHLNI 190 Query: 182 GAGL 185 A L Sbjct: 191 AAAL 194 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.132 0.371 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 620,037 Number of extensions: 22984 Number of successful extensions: 110 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 109 Number of HSP's successfully gapped: 4 Length of query: 214 Length of database: 1,619,870 Length adjustment: 81 Effective length of query: 133 Effective length of database: 1,236,335 Effective search space: 164432555 Effective search space used: 164432555 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 128 (53.9 bits)