BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0210.0019.p
         (259 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300311855|ref|YP_003775947.1| sphingosine kinase/eukaryotic d...   332   1e-92

>gi|300311855|ref|YP_003775947.1| sphingosine kinase/eukaryotic
           diacylglycerol kinase [Herbaspirillum seropedicae SmR1]
          Length = 339

 Score =  332 bits (852), Expect = 1e-92,   Method: Compositional matrix adjust.
 Identities = 165/227 (72%), Positives = 186/227 (81%), Gaps = 12/227 (5%)

Query: 6   QAAFGVLPLGTLNHFVKDLNIPLALDDAIRNIVEGRRLAIDTGEVNGVLFINNSSLGLYP 65
           Q+A GVLPLGTLNHF KDLNIPL L+ AI NI  GRRL +D+ EVNGV FINNSSLGLYP
Sbjct: 100 QSALGVLPLGTLNHFAKDLNIPLTLEGAIANIATGRRLRVDSAEVNGVAFINNSSLGLYP 159

Query: 66  DIVRDREKQQSRLGRGKWLAFCWAAMGALRRYPFLDVRMDIRDDGDNAESHRRRTPFVFI 125
           DIVR+REKQQ+RLGRGKWLAF WAAMGALRRYPFL VR+ I     + + H RRTPFVFI
Sbjct: 160 DIVREREKQQARLGRGKWLAFTWAAMGALRRYPFLRVRLSI-----DGQEHWRRTPFVFI 214

Query: 126 GNNEYLMDGFNIGKRNSLSEGKLSLYVCHRTGRLGLVRLALHALFGRLEQARDFDILSAA 185
           GNN YLM G +IGKR+SL+EG LSLYVCH TGR GL+RLALHALFGRL QA DFD+L+A 
Sbjct: 215 GNNPYLMSGLDIGKRSSLTEGCLSLYVCHHTGRWGLLRLALHALFGRLRQAHDFDMLTAT 274

Query: 186 EIEIDTRHGHGRHRVKRVRVATDGEVTIMQTPLVYRIRPASLTVIVP 232
           +I I+T      H+ +R+RVATDGEV +MQTPL YRIRPASL VIVP
Sbjct: 275 DIHIET------HK-RRMRVATDGEVQLMQTPLSYRIRPASLEVIVP 314


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.322    0.139    0.408 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 768,700
Number of extensions: 30376
Number of successful extensions: 66
Number of sequences better than 1.0e-08: 1
Number of HSP's gapped: 63
Number of HSP's successfully gapped: 1
Length of query: 259
Length of database: 1,619,870
Length adjustment: 83
Effective length of query: 176
Effective length of database: 1,226,865
Effective search space: 215928240
Effective search space used: 215928240
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 129 (54.3 bits)