BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0207.0019.p (443 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312683|ref|YP_003776775.1| major facilitator superfamily p... 650 0.0 gi|300310856|ref|YP_003774948.1| major facilitator superfamily p... 259 4e-70 gi|300311040|ref|YP_003775132.1| major facilitator superfamily p... 242 4e-65 gi|300313188|ref|YP_003777280.1| major facilitator superfamily p... 224 9e-60 gi|300312017|ref|YP_003776109.1| multidrug resistance B (translo... 144 1e-35 >gi|300312683|ref|YP_003776775.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 498 Score = 650 bits (1676), Expect = 0.0, Method: Compositional matrix adjust. Identities = 338/444 (76%), Positives = 375/444 (84%), Gaps = 1/444 (0%) Query: 1 LWIVASGFFMQTLDTTIVNTALPSMAASLGEPVLAMHPVVVSYTLTMASLTPASGWLADR 60 LWIVASGFFMQTLDTTIVNTALP+MA SLGEPVL MHPVVV+YTLTMASLTPASGWLADR Sbjct: 55 LWIVASGFFMQTLDTTIVNTALPAMARSLGEPVLNMHPVVVAYTLTMASLTPASGWLADR 114 Query: 61 FGTRRVYFLAILLFVLGSLLCANAHSLPQLLLARVVQGVGGSMLLPIGRLAVLRSVPGAQ 120 FGTRR+YF AILLF LGSLLCA A SLP LL ARVVQG+GGSMLLP+GRLAVLR+VPGAQ Sbjct: 115 FGTRRMYFAAILLFSLGSLLCAMASSLPMLLAARVVQGLGGSMLLPVGRLAVLRAVPGAQ 174 Query: 121 YISALAFISIAGQLGPVVGPVTGGWLSQTIGWHWIFLINVPVGVIGMFAVHRFLPGEEIT 180 YI+ALA ISIAGQLGPVVGPV GGWL +++ WHWIFLIN+PVGV+GM AV FLP + + Sbjct: 175 YIAALASISIAGQLGPVVGPVLGGWLVESLSWHWIFLINLPVGVLGMGAVLYFLPDDSLA 234 Query: 181 SPPRFDMFGCALLSTCMVTFSLALDVPMDEHRTWWSAGLFGISVLTVWRYVIHARRDEQP 240 PRFD+ GC LLS CMV FSLALDVP D HRT WS LF ++ LTV Y HA +P Sbjct: 235 GTPRFDLLGCVLLSACMVAFSLALDVPFDSHRTGWSIALFVVAALTVLGYWRHASVHPRP 294 Query: 241 LFRLGLFNEPNFSVGLIGNLVCRIGSSAVPFLLPLLLQVKLGFSPLHAGLVLLPAAVAGT 300 LFRLGLF EPNFS+GL GNLVCRIGSSAVPFLLPLLLQV+LG+SPLHAG+VLLPAA+AGT Sbjct: 295 LFRLGLFGEPNFSIGLAGNLVCRIGSSAVPFLLPLLLQVRLGYSPLHAGMVLLPAAIAGT 354 Query: 301 IAKPWIAPLVRRYGYENFLLVNTIMVGAAIVSFALITPAWPIWIGIVQLAVFGVCNSMQF 360 IAKPWIAPLV+R+GY+ FLLVNT +VGAAI+SFALITP WP+ IVQLA+FG NSMQF Sbjct: 355 IAKPWIAPLVKRFGYDRFLLVNTAIVGAAIMSFALITPDWPLTAAIVQLAIFGASNSMQF 414 Query: 361 AAMNSVTLKGLDSKDAGSGNSLFSMVQMLALGLGVTIAGSMLNGLDG-VAWAREWSFTLT 419 AAMNSVTLKGLD +DAGSGNSLFSMVQMLA+GLGVTI G+M+N V A SF +T Sbjct: 415 AAMNSVTLKGLDKRDAGSGNSLFSMVQMLAIGLGVTIGGTMVNLFKSEVGTATNASFQIT 474 Query: 420 FVCVGIVTLLSALVFRRMDARYFN 443 F VG +TL SA VFR+MD RYF Sbjct: 475 FAIVGAITLASAWVFRKMDPRYFK 498 >gi|300310856|ref|YP_003774948.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 446 Score = 259 bits (661), Expect = 4e-70, Method: Compositional matrix adjust. Identities = 148/435 (34%), Positives = 242/435 (55%), Gaps = 16/435 (3%) Query: 13 LDTTIVNTALPSMAASLGEPVLAMHPVVVSYTLTMASLTPASGWLADRFGTRRVYFLAIL 72 +D+T+++T+LP++A + +++ + SY +++A P SGW+ADRFG+ ++ AI Sbjct: 1 MDSTVISTSLPALALDMNVDPISLKLALTSYLVSLAVFIPISGWMADRFGSLTIFRSAIC 60 Query: 73 LFVLGSLLCANAHSLPQLLLARVVQGVGGSMLLPIGRLAVLRSVPGAQYISALAFISIAG 132 +F+LGS+LC + +LPQ + AR +QG+GG+M++P+GRL +LRSV + AL++++I Sbjct: 61 VFMLGSILCGVSSTLPQFVAARFLQGIGGAMMVPVGRLVILRSVEKVNLVKALSYLTIPA 120 Query: 133 QLGPVVGPVTGGWLSQTIGWHWIFLINVPVGVIGMFAVHRFLPGEEITSPPRFDM----- 187 GPV+GP GG+++ W WIF IN+P+ ++G++ RF+P + D+ Sbjct: 121 LFGPVLGPPLGGFITTYFHWRWIFFINIPISILGLYLATRFIPDLKAEQVAPLDLKGFLL 180 Query: 188 --FGCALLSTCMVTFSLALDVPMDEHRTWWSAGLFGISVLTVWRYVIHARRDEQPLFRLG 245 GC+LL + T L V + T AGL ++ YV HARR PL L Sbjct: 181 SALGCSLLMMGLATAGRHL-VSGEVALTCVLAGLVFLAA-----YVWHARRASAPLIDLA 234 Query: 246 LFNEPNFSVGLIGNLVCRIGSSAVPFLLPLLLQVKLGFSPLHAGLVLLPAAVAGTIAKPW 305 L P G+ G R+G A+PFLLPL+LQ+ G++P H+G++ +A K Sbjct: 235 LLKIPTLRAGVFGGSFYRVGIGAIPFLLPLMLQLGFGYTPFHSGMLTCASAAGAMFMKTI 294 Query: 306 IAPLVRRYGYENFLLVNTIMVGAAIVSFALITPAWPIWIGIVQLAVFGVCNSMQFAAMNS 365 A +RRYG+ LLVN + + + A TP P + +V L + G S+QF ++N+ Sbjct: 295 AARFLRRYGFRRVLLVNGALGVLGLWAIATFTPQTPYLLMVVILLIGGCFRSLQFTSLNA 354 Query: 366 VTLKGLDSKDAGSGNSLFSMVQMLALGLGVTIAG---SMLNGLDGVAWAREWSFTLTFVC 422 ++ +D S+ S+ Q L++G+GV +AG M N + G A F F+ Sbjct: 355 ISYADVDKLRLSQATSMASVAQQLSIGMGVVLAGFALQMSNEIQGHATLVAQDFWPAFLV 414 Query: 423 VGIVTLLSALVFRRM 437 VG V + L+ R++ Sbjct: 415 VGAVAACALLMLRQL 429 >gi|300311040|ref|YP_003775132.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 441 Score = 242 bits (618), Expect = 4e-65, Method: Compositional matrix adjust. Identities = 138/423 (32%), Positives = 235/423 (55%), Gaps = 6/423 (1%) Query: 13 LDTTIVNTALPSMAASLGEPVLAMHPVVVSYTLTMASLTPASGWLADRFGTRRVYFLAIL 72 +D T++ T+LP++A L + + + + +Y + P SGW+AD+ G R+V+ A++ Sbjct: 1 MDATVLATSLPALARDLHQDPITLKLAMTAYVAALGVFIPISGWMADKLGARKVFSAAMI 60 Query: 73 LFVLGSLLCANAHSLPQLLLARVVQGVGGSMLLPIGRLAVLRSVPGAQYISALAFISIAG 132 +F+ GS+LCA + SL + AR +QG+GG+M++P+GR+ + RSV ++ + A++++++ Sbjct: 61 VFMTGSILCAVSGSLVTFVAARFLQGIGGAMMVPVGRVIIARSVDKSELVKAVSYLTLPA 120 Query: 133 QLGPVVGPVTGGWLSQTIGWHWIFLINVPVGVIGMFAVHRFLPGEEITSPPRFDMFGCAL 192 LGPVVGP+ GG+++ W W+FLIN+P +G++ RF+ + +P D+ G L Sbjct: 121 LLGPVVGPLLGGFITTYFHWRWVFLINIPFCFLGLYLARRFIQNFKEDNPAPLDVKGFVL 180 Query: 193 LSTCMVTFSLALDVPMDEHRTWWSA--GLFGISVLTVWRYVIHARRDEQPLFRLGLFNEP 250 S L L + H + + I + + Y H+ + E PL L P Sbjct: 181 TSVGAAMIMLGLSL-FGSHLVDGATPVAMCSIGAIALVMYYRHSTQIEFPLLDFRLLRIP 239 Query: 251 NFSVGLIGNLVCRIGSSAVPFLLPLLLQVKLGFSPLHAGLVLLPAAVAGTIAKPWIAPLV 310 ++G + RIG AVPFLLPL LQ LG + AG + +A + ++ Sbjct: 240 TLHASVVGGSLFRIGLGAVPFLLPLALQEGLGMTAFQAGSITCASAFGSLFIRLLAGRVL 299 Query: 311 RRYGYENFLLVNTIMVGAAIVSFALITPAWPIWIGIVQLAVFGVCNSMQFAAMNSVTLKG 370 RRYG+ L+VN + G A+ ++ + + P + + + + GV ++QF +NS+ Sbjct: 300 RRYGFRTVLMVNAVFAGIALAAYGIFSIHTPYLLIVSIVLMGGVFPALQFTCLNSIAYAD 359 Query: 371 LDSKDAGSGNSLFSMVQMLALGLGVTIAGSML---NGLDGVAWAREWSFTLTFVCVGIVT 427 +DS+DAG SL S++Q L+LGLGVT+AG +L N L G A E F F+ +G+ + Sbjct: 360 IDSRDAGRVTSLASVIQQLSLGLGVTVAGIVLQLSNHLQGHATIVEADFWPAFLVIGLFS 419 Query: 428 LLS 430 + S Sbjct: 420 VAS 422 >gi|300313188|ref|YP_003777280.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 498 Score = 224 bits (572), Expect = 9e-60, Method: Compositional matrix adjust. Identities = 146/451 (32%), Positives = 240/451 (53%), Gaps = 24/451 (5%) Query: 2 WIVASGFFMQTLDTTIVNTALPSMAASLGEPVLAMHPVVVSYTLTMASLTPASGWLADRF 61 +IVA+ FFM+ LDTTI+ TALP MA S G + + +Y LT+A P SGW+ADRF Sbjct: 34 YIVAATFFMEYLDTTIIATALPQMAQSFGVGPNELSLGMTAYMLTLAVFIPISGWVADRF 93 Query: 62 GTRRVYFLAILLFVLGSLLCANAHSLPQLLLARVVQGVGGSMLLPIGRLAVLRSVPGAQY 121 G+R V+ +AI +F L S+LC + ++ + ARV+QG+GG+M++P+GR+ V+RS + Sbjct: 94 GSRTVFGMAIAIFTLASILCGFSQNVWEFTAARVLQGLGGAMMVPVGRMIVVRSTGKDRL 153 Query: 122 ISALAFISIAGQLGPVVGPVTGGWLSQTIGWHWIFLINVPVGVIGMFAVHRFLPGEEITS 181 + A+A I+ + PVVGP GG+++ WHWIFL+NVP G+ AV + E S Sbjct: 154 MKAIATITWPAIVAPVVGPSIGGFITTYASWHWIFLMNVPFGLAAFTAVMMVVRNEHGVS 213 Query: 182 PPRFDMFGCALLSTCMVTFSLALDVPMD-EHRTWWSAGLFGISVL---TVWRYVIHARRD 237 D+ G L + + ++ E + G + V+ WR H +R Sbjct: 214 KRPLDLVGFLLSGAALTSLLYGTEIASHMESNIKVALGFVALGVVLGYGAWR---HMQRI 270 Query: 238 EQPLFRLGLFNEPNFSVGLIGNLVCRIGSSAVPFLLPLLLQVKLGFSPLHAGLVLLPAAV 297 E PL P +SV ++ + RIG AVP+L+PL+ Q+ G + +GL+LL A+ Sbjct: 271 EHPLLDFSNLRIPTYSVTVLTGSLTRIGVDAVPYLMPLMFQIGFGLTAFQSGLLLLATAL 330 Query: 298 AGTIAKPWIAPLVRRYGYENFLLVNTIMVGAAIVSFALITPAWPIWIGIVQLAVFGVCNS 357 K + +++R+G+ + + N + G I++ L+TPA P+ + + + +G+ S Sbjct: 331 GNLGMKAFTTRILQRFGFRSVAVNNAAIAGGLIIACGLLTPATPLPVILGVIFCYGLSRS 390 Query: 358 MQFAAMNSVTLKGLDSKDAGSGNSLFSMVQMLALGLGVTIAGSML--------NGLDGVA 409 MQF+ + ++ + S ++L+S+ Q + +G+G+ L +D Sbjct: 391 MQFSTLATLAYADISDARKSSASTLWSVAQQMTIGMGIAFGALALRIAAALRGEAID--- 447 Query: 410 WAREWSFTL-----TFVCVGIVTLLSALVFR 435 A FTL F G+VTL+S + +R Sbjct: 448 -ASGAHFTLGDFQWAFAMAGVVTLISVIGYR 477 >gi|300312017|ref|YP_003776109.1| multidrug resistance B (translocase) transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 524 Score = 144 bits (363), Expect = 1e-35, Method: Compositional matrix adjust. Identities = 129/431 (29%), Positives = 203/431 (47%), Gaps = 57/431 (13%) Query: 4 VASGFFMQTLDTTIVNTALPSMAASLGEPVLAMHPVVVSYTLTMASLTPASGWLADRFGT 63 V+ FM LD++I N A+P+++ +LG V V+ S+ A P +GWL R G Sbjct: 29 VSLAVFMNVLDSSIANVAIPTISGNLGVSVDEGTWVITSFAAANAISIPLTGWLTQRLGA 88 Query: 64 RRVYFLAILLFVLGSLLCANAHSLPQLLLARVVQGVGGSMLLPIGRLAVLRSVPGAQYIS 123 R++ ++LLFVL S LC A +LP LL ARV+QG+ ++P+ + +L + P ++ Sbjct: 89 VRLFVTSVLLFVLASWLCGLAPTLPILLAARVLQGLVAGPMVPLSQSLLLGAYPKSKSSF 148 Query: 124 ALAFISIAGQLGPVVGPVTGGWLSQTIGWHWIFLINVPVGVIG---MFAVHRFLPGEEIT 180 ALA + + PV GP GGW++ + W WIF IN+PVG+I A++R E T Sbjct: 149 ALALWGMTAVVAPVAGPALGGWITDSYTWPWIFYINIPVGLIAAGVTLAIYR--SRETPT 206 Query: 181 SPPRFDMFGCALLSTCMVTFSLALDVPMDEHRTWWSAG---LFGISVLTVWRY-VIHARR 236 D G ALL T + F + LD D W+++ GI L + Y V+ Sbjct: 207 HKLPIDKIGLALLVTWVAAFQIMLDKGKDLD--WFASPTIVTLGIIALIAFAYFVVWELN 264 Query: 237 DEQPLFRLGLFNEPNFSVGLI----------GNLVCRIGSSAVPFLLPLLLQVKLGFSPL 286 ++ P+ L LF NF+ G I GNLV +LP LQ LG+ + Sbjct: 265 NDHPVVDLRLFGRRNFAGGTIAISIGYGVFFGNLV----------ILPQWLQQYLGYRSI 314 Query: 287 HAGLVLLPAAVAGTIAKPWIAPLVRRYGYENFLLVNTIMVG-AAIVSFALITPAWPIW-- 343 +GL P + I + PL+ R+ LV+ V A+ + FA++ W Sbjct: 315 DSGLSTAPIGIFAVI----LMPLIGRF----LPLVDARKVATASFIGFAIV-----FWLR 361 Query: 344 ----IGIVQLAVF------GVCNSMQFAAMNSVTLKGLDSKDAGSGNSLFSMVQMLALGL 393 + + Q+ V G+ +M F + + L GL + + L S V++ + Sbjct: 362 SRYNLQVDQMTVILPTLLQGIPTAMFFVPLTVLLLSGLPPERIPAAAGLSSFVRVFCGAV 421 Query: 394 GVTIAGSMLNG 404 G +I+ + N Sbjct: 422 GTSISTTAWNN 432 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.141 0.448 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,388,870 Number of extensions: 59524 Number of successful extensions: 400 Number of sequences better than 1.0e-08: 17 Number of HSP's gapped: 377 Number of HSP's successfully gapped: 17 Length of query: 443 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 355 Effective length of database: 1,203,190 Effective search space: 427132450 Effective search space used: 427132450 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 132 (55.5 bits)