BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0207.0015.p (550 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312681|ref|YP_003776773.1| TonB-dependent siderophore rece... 654 0.0 gi|300309529|ref|YP_003773621.1| TonB dependent ferrisiderophore... 161 2e-40 gi|300313664|ref|YP_003777756.1| tonB-dependent receptor for iro... 151 1e-37 gi|300312552|ref|YP_003776644.1| TonB-dependent outer membrane i... 150 3e-37 gi|300310762|ref|YP_003774854.1| outer membrane ferric sideropho... 129 8e-31 >gi|300312681|ref|YP_003776773.1| TonB-dependent siderophore receptor protein [Herbaspirillum seropedicae SmR1] Length = 738 Score = 654 bits (1688), Expect = 0.0, Method: Compositional matrix adjust. Identities = 333/437 (76%), Positives = 369/437 (84%), Gaps = 13/437 (2%) Query: 1 MNRLTPIAAALVAVFAAPVAVHAQQ---TSSPAAAPAPANAAAPSSSLKEVVVEGKRDDF 57 MNRLTPIAAALVA FAAP+++HAQQ T++ A PA AP V V+G+RDDF Sbjct: 1 MNRLTPIAAALVATFAAPLSLHAQQVSPTTTSAGKPAADGQLAP------VTVQGQRDDF 54 Query: 58 NTATTALTKLPADLHDVPQSVVVVNKALLQSQGATSLSDALRNVAGITLGGAEGGQIGNN 117 + TALTKLPADL DVPQSV VVNKA +QSQGA+SLSDALRNV+GITLGGAEGGQIGNN Sbjct: 55 ASTGTALTKLPADLRDVPQSVTVVNKAQMQSQGASSLSDALRNVSGITLGGAEGGQIGNN 114 Query: 118 INLNGFSARTDIYLDGFRDRAQYYRDTFAMDEIEVLMGPSSMLFGRGSTGGIINQVTKKA 177 INLNGFSARTDIYLDGFRDR QYYRDTFA+DE+EVLMGPSSMLFGRGSTGGIINQV+KKA Sbjct: 115 INLNGFSARTDIYLDGFRDRGQYYRDTFAIDEVEVLMGPSSMLFGRGSTGGIINQVSKKA 174 Query: 178 NLKESTEVSASVTTNGLVRTTVDHNQPLSDTSAFRISAMAQDGAASTRNQINVQDFGLAA 237 NLK +TE+S S+TTNGLVR+TVD N+PLSDTSA RI AMAQ+GAASTR Q +VQDFGLA Sbjct: 175 NLKAATEISGSITTNGLVRSTVDVNRPLSDTSALRIEAMAQNGAASTRKQTDVQDFGLAG 234 Query: 238 SYVTGLGTSTEITLSALIQHNHDQPDYGLPPLNGHPVGVDRDTAYGLNSDRTDQDVVSLN 297 SYV G+GT TEITLSAL+QHN DQPDYGLPPLNGHP V RDTAYGLNSDRT QDV S N Sbjct: 235 SYVWGIGTPTEITLSALLQHNQDQPDYGLPPLNGHPANVGRDTAYGLNSDRTIQDVASFN 294 Query: 298 AGIKHKLTPDFTIRNQTQFNYVHTNAVETAPNTIGTVSGAGFTALTGAASSLPLSSLYVR 357 G+KHK+ PD T+RNQTQFNYVHT+AVETAPNTIGTVSGAGFT L A+S+LPLSSL+VR Sbjct: 295 VGVKHKIAPDVTLRNQTQFNYVHTDAVETAPNTIGTVSGAGFTQLVNASSNLPLSSLFVR 354 Query: 358 AQSHDRDIHDYSIFNQTELTGKIG---FKHDLVVGLEVGHDGYDNQAYYRNGSCNGVALT 414 QSHDR I DYSIFNQTELT K KHD VVG+EVGHDGYDNQ YYRNGSCNG AL Sbjct: 355 GQSHDRVITDYSIFNQTELTAKFATGSIKHDAVVGVEVGHDGYDNQNYYRNGSCNGTALN 414 Query: 415 GAATPTSGYADCIPVVS 431 A T+GY DC+P+++ Sbjct: 415 -ANGATTGYVDCVPLLN 430 Score = 78.6 bits (192), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 34/50 (68%), Positives = 40/50 (80%), Gaps = 1/50 (2%) Query: 446 FKHDLVVGLEVGHDGYDNQAYYRNGSCNGVALTGAATPTSGYADCIPVVS 495 KHD VVG+EVGHDGYDNQ YYRNGSCNG AL A T+GY DC+P+++ Sbjct: 382 IKHDAVVGVEVGHDGYDNQNYYRNGSCNGTALN-ANGATTGYVDCVPLLN 430 >gi|300309529|ref|YP_003773621.1| TonB dependent ferrisiderophore receptor protein [Herbaspirillum seropedicae SmR1] Length = 704 Score = 161 bits (407), Expect = 2e-40, Method: Compositional matrix adjust. Identities = 124/411 (30%), Positives = 208/411 (50%), Gaps = 48/411 (11%) Query: 1 MN-RLTPIAAALVAVFA-APVAVHAQQTSSPAAAPAPANAAAPSSSLKEVVVEGKR-DDF 57 MN RL+P+ A++A + +P+A+ PA A +++L + V G R +D Sbjct: 1 MNFRLSPLRLAVLATCSLSPLAI------------LPACAQQDTTALPAIEVSGARANDG 48 Query: 58 NTATTAL--TKLPADLHDVPQSVVVVNKALLQSQGATSLSDALRNVAGITLGGAEGGQIG 115 A TA+ TK A L DVPQ++ VV +++ Q A+SL D L+NV G+ L +G + Sbjct: 49 YVAPTAVSGTKSEAPLRDVPQTINVVPAQVIRDQNASSLQDVLKNVPGLGLSTGDGQR-- 106 Query: 116 NNINLNGFSARTDIYLDGFRDRAQYYRDTFAMDEIEVLMGPSSMLFGRGSTGGIINQVTK 175 + + + GF+A D ++DGFRD A Y+RD ++ +EV+ GP+++L+GRGS+GG+IN+VTK Sbjct: 107 DQVFIRGFTAIGDQFVDGFRDDALYFRDLSNVESVEVIKGPAAVLYGRGSSGGLINRVTK 166 Query: 176 KANLKESTEVSASVTTNGLVRTTVDHNQPLSDTSAFRISAMAQDGAASTRNQINVQDFGL 235 K + + ++VS + + R +D + ++R++ + G S R Q + + Sbjct: 167 KPGV-DVSDVSFTANSTAGRRAELDLGRAGEGALSWRLTGAVERG-DSFRAQQFIDRTAI 224 Query: 236 AASYVTGLGTSTEITLSALIQHNHDQPDYGLPPLNGHPVGVDRDTAYG----LNSDRTDQ 291 A S T++ L + D+G+P G PV VD T YG ++D Sbjct: 225 APSVALRFSADTKLLLQMDYLEDRRMMDFGIPSYRGRPVDVDPGTTYGARNARDADFVQS 284 Query: 292 DVVSLNAGIKHKLTPDFTIRNQTQFNYVHTNAVETAPNTIGTVSGAGFTALTGAASSLPL 351 V S A H+ +F+ RN ++ H N T T+ + A ++L Sbjct: 285 RVASTTATFTHRFNDEFSFRNGLRYYDYHLNRNNTNGGTVNEAT---------ATTTL-- 333 Query: 352 SSLYVRAQSHDRDIHDYS-IFNQTELTGKI---GFKHDLVVGLEVGHDGYD 398 +H + + D + FNQ+ELT K+ +H+++ GLE+G D Sbjct: 334 --------NHAKLVRDENGWFNQSELTQKLQLGSIRHEILYGLELGSQSKD 376 >gi|300313664|ref|YP_003777756.1| tonB-dependent receptor for iron transport protein [Herbaspirillum seropedicae SmR1] Length = 798 Score = 151 bits (381), Expect = 1e-37, Method: Compositional matrix adjust. Identities = 127/393 (32%), Positives = 185/393 (47%), Gaps = 34/393 (8%) Query: 38 AAAP---SSSLKEVVVEGKRD--DFNTATTALTKLPADLHDVPQSVVVVNKALLQSQGAT 92 A AP S LKEVVV R+ ++ + K L D+PQS+ VV K +L Q A Sbjct: 51 AVAPPEDKSGLKEVVVTAPREAPEYKAQAVSSPKFTQPLLDLPQSITVVPKEVLTQQNAQ 110 Query: 93 SLSDALRNVAGITLGGAEGGQ-IGNNINLNGFSARTDIYLDGFRDRAQYYR-DTFAMDEI 150 +L D L+NV GIT EG G+ + GFS+ + +DG RD R DTF ++++ Sbjct: 111 NLQDILKNVPGITFTSGEGNLGWGDMFTIRGFSSEQSLTVDGVRDAGMSSRNDTFNLEQV 170 Query: 151 EVLMGPSSMLFGRGSTGGIINQVTKKANLKESTEVSASVTTNGLVRTTVDHNQPLSDTSA 210 EV G S+ G + GG +N V+K+A L + S V + G R T D N L TSA Sbjct: 171 EVFKGTGSIESGVSAIGGSVNLVSKEARLDDFYNTSFGVGSAGYTRFTADLNHQLDATSA 230 Query: 211 FRISAMAQDGAASTRNQINVQDFGLAASYVTGLGTSTEITLSALIQHNHDQPDYGLPPLN 270 R++ M Q + R+ + G+AAS GLGT T I Q + + PD GLP Sbjct: 231 LRLNLMKQQNHVAERDVVKYDRQGIAASLGLGLGTRTRIFFDVFHQDDDNIPDTGLPIQR 290 Query: 271 G-----HPVGVDRDTAYGLNSDRTDQDVVSLNAGIKHKLTPDFTIRNQTQFNY------- 318 G P V R+ YG + +L A ++H + IRNQT++ Sbjct: 291 GTGGSVMPY-VSRNAWYGSTTYTQQTTTDALTARVEHDFSDAVKIRNQTRWERADNLSVL 349 Query: 319 --VHTNAVETAPNTIGTVSG-AGFTALTGAASSLPLSSL------YVRAQSHDRDI-HDY 368 NA +A ++GT G AG +LT A+ L S Y + +D Y Sbjct: 350 SPARFNAANSAGASLGTSLGYAGSGSLTAASGILSYSDFTNTANRYGVLRGNDFGTSKRY 409 Query: 369 SIF-NQTELTGKI---GFKHDLVVGLEVGHDGY 397 +I NQ++L+ K +H+L G+++ + Y Sbjct: 410 TILDNQSDLSLKFDTGAIRHELSTGVDLYRETY 442 >gi|300312552|ref|YP_003776644.1| TonB-dependent outer membrane iron monomeric catechols receptor protein [Herbaspirillum seropedicae SmR1] Length = 799 Score = 150 bits (379), Expect = 3e-37, Method: Compositional matrix adjust. Identities = 130/431 (30%), Positives = 196/431 (45%), Gaps = 42/431 (9%) Query: 5 TPIAAALVAVFAAPVAVHAQQTSSPAAAPAPANAAAPSSSLKEVVVEGKRDDFNTATTAL 64 T ++AA+ + P A+ AQ S ANAA PS V + + + Sbjct: 46 TSLSAAVASALLLP-AIGAQAQS--------ANAALPSVE----VTASQEPAYKADKASS 92 Query: 65 TKLPADLHDVPQSVVVVNKALLQSQGATSLSDALRNVAGI---TLGGAEGGQIGNNINLN 121 K L D PQ++ V+ + L+ QGAT+L++ALRN G+ +LG G+ I + Sbjct: 93 AKYTQPLVDTPQTLTVIKRQLIDQQGATTLTEALRNSPGVGTFSLGENGATNTGDAIYMR 152 Query: 122 GFSARTDIYLDGFRDRAQYYRDTFAMDEIEVLMGPSSMLFGRGSTGGIINQVTKKANLKE 181 GF + + I++D RD RD F + ++EVL GP+ GRGS G IN VTK+A + Sbjct: 153 GFDSSSAIFVDSVRDLGSVSRDMFNIQQVEVLKGPAGTDTGRGSPTGSINLVTKQAETTD 212 Query: 182 STEVSASVTTNGLVRTTVDHNQPLSDT--SAFRISAMAQDGAASTRNQINVQDFGLAASY 239 + SA + R T D N+ L+ +A R++ M QD R+++ GLA S Sbjct: 213 AFSASAMYGSWNQKRVTADWNKVLNAETGTAMRLNLMKQDSGVPGRHEVKNDRTGLALSL 272 Query: 240 VTGLGTSTEITLSALIQHNHDQPDYGLPP--LNGHPV------------GVDRDTAYGLN 285 GLG+ T + L L D PD G+P L G+ VD YGL Sbjct: 273 AHGLGSDTRVFLDYLHMEQDDVPDGGVPTIGLPGYTTPDATRRFLSSAGRVDSSNFYGLT 332 Query: 286 SDRTDQDVVSLNAGIKHKLTPDFTIRNQTQFNYVHTNAVETAPNTIGTVSGAGFTALTGA 345 SD + L ++H +PD ++N T+ H N + TA S T Sbjct: 333 SDYNQVNTDRLTLRVEHDFSPDLKLQNTTRIGKTHQNYLLTA---FMASSANLLTPNAND 389 Query: 346 ASSLPLSSLYVRAQSHDRDIHDYSIFNQTELTGKI---GFKHDLVVGLEVGHDGYDNQAY 402 ASS L+ R+ ++ + I NQT LT K+ +H L+ G+E+ + +N Sbjct: 390 ASSWTLA----RSLPTIKNQTNTIITNQTYLTAKLKSGSLEHTLMGGVELTQERQNNSGV 445 Query: 403 YRNGSCNGVAL 413 +G+ V L Sbjct: 446 LTSGTLPAVNL 456 >gi|300310762|ref|YP_003774854.1| outer membrane ferric siderophore receptor protein [Herbaspirillum seropedicae SmR1] Length = 717 Score = 129 bits (323), Expect = 8e-31, Method: Compositional matrix adjust. Identities = 123/415 (29%), Positives = 196/415 (47%), Gaps = 60/415 (14%) Query: 5 TPIAAALVAVFAAPVAVHAQQTSSPAAAPAPANAAAPS--SSLKEVVVEGKR---DDFNT 59 +PI A V A + + + AA N PS SSL V V +R D Sbjct: 6 SPIPCACVRRLPATLCLAFLALPAYAAERDGGNEGEPSGESSLPVVEVRDQRRSPDPRAR 65 Query: 60 ATTALTKLPADLHDVPQSVVVVNKALLQSQGATSLSDALRNVAGITLGGAEGGQIGNNIN 119 T+ AD +VPQS+ V+ + GA +L+ AL V GI+ + + Sbjct: 66 RVGTATRSAADPMEVPQSIQSVSVEQVSVYGARTLAGALLGVPGIS---SISDTRFDAFR 122 Query: 120 LNGFSARTDIYLDGFRDRAQYYRDTFAMDEIEVLMGPSSMLFGRGSTGGIINQVTKKANL 179 + GFS+ D+ LDG RD QY R ++ +EVL GP+S+L+GRGS GG+IN+++K+ Sbjct: 123 IRGFSSANDLLLDGMRDDPQYVRGLGNIERVEVLRGPASVLYGRGSGGGVINRISKQPGQ 182 Query: 180 KESTEVSASVTTNGLVRTTVDHNQPLSDTSAFRISAMAQDGAASTRNQIN--VQDFGLAA 237 +++ + S+ + GL+ +D N+PL+ A RI+ ++ A S R+ +N Q F A Sbjct: 183 DVASQATLSLGSAGLLGAALDWNRPLAPHWALRINT-GREHAGSFRDTVNGTRQYFAPAL 241 Query: 238 SYVTGLGTSTEITLSALIQHNHDQ----PDYGLP-----PLNGHPVGVDRDTAYGLNSDR 288 + +G S L+Q +D+ PD G+P NG P TAY L S Sbjct: 242 KWESGRD-------SWLLQAEYDEYERVPDRGIPGRAIATANGEP------TAYALPSAG 288 Query: 289 TDQ-------DVV-----SLNAGIKHKLTPDFTIRNQTQFNYVHTNAVETAPNTIGTVSG 336 +Q DV+ SL + + L+ D+T+R+ +++N Sbjct: 289 HEQFFGAAGRDVIRDIHMSLRSTLTRALSQDWTLRHALALMDLNSNFDN----------- 337 Query: 337 AGFTALTGAASSLPLSSLYVRAQSHDRDIHDYSIFNQTELTGKIGFKHDLVVGLE 391 T +T A S PL V+ + +++ S+ N EL G++G HDL++G+E Sbjct: 338 ---TFVTQAYQSAPLDLDRVQRTRYQQNLQQRSVQNNLELLGQLG-AHDLLLGVE 388 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.313 0.129 0.371 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,531,785 Number of extensions: 61296 Number of successful extensions: 253 Number of sequences better than 1.0e-08: 16 Number of HSP's gapped: 231 Number of HSP's successfully gapped: 17 Length of query: 550 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 460 Effective length of database: 1,193,720 Effective search space: 549111200 Effective search space used: 549111200 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 133 (55.8 bits)