BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0204.0012.p (607 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311236|ref|YP_003775328.1| hypothetical protein Hsero_1911... 1059 0.0 gi|300313035|ref|YP_003777127.1| serine phosphatase, regulator o... 101 2e-22 gi|300309883|ref|YP_003773975.1| GGDEF domain/EAL domain signal ... 100 5e-22 gi|300311038|ref|YP_003775130.1| hypothetical protein Hsero_1709... 92 1e-19 gi|300312012|ref|YP_003776104.1| phosphodiesterase transmembrane... 84 3e-17 >gi|300311236|ref|YP_003775328.1| hypothetical protein Hsero_1911 [Herbaspirillum seropedicae SmR1] Length = 600 Score = 1059 bits (2738), Expect = 0.0, Method: Compositional matrix adjust. Identities = 494/590 (83%), Positives = 546/590 (92%) Query: 18 LLSHLHEIIAQRSLTALFQPIIRMRSGEIIGYEGLIRGPSNSPLHSPLNLFKVARANKLS 77 +LS LHEIIAQR LTALFQPI+RMR+GEI+GYEGLIRGPSN+PLHSPL LFKVARA+ +S Sbjct: 8 MLSLLHEIIAQRKLTALFQPIVRMRNGEILGYEGLIRGPSNTPLHSPLALFKVARAHNMS 67 Query: 78 VQVEHLCRHIVMAQFAQSGLPGNLFLNVSPGALVQPEAIQGETLRHLEKLGINPQRIIIE 137 VQ+E+LCRHIV+ +FAQ GLPGNLFLNVSPGAL+QPEAIQGETLR+L+ LG+NPQRIIIE Sbjct: 68 VQMEYLCRHIVLTRFAQLGLPGNLFLNVSPGALMQPEAIQGETLRYLDTLGVNPQRIIIE 127 Query: 138 LTENEPTYDYTLLRNAAMHYRDMGFQIAIDDLGEGFSSLRLWSELQPEYVKVDMHFIQGI 197 LTENEPTYDY LRNAAMHYRDMGFQIAIDDLGEGFSSLRLWSELQPEYVKVDMHF+QGI Sbjct: 128 LTENEPTYDYAQLRNAAMHYRDMGFQIAIDDLGEGFSSLRLWSELQPEYVKVDMHFVQGI 187 Query: 198 NNDPMKLQFVRSIQEIAEKSGSIVIAEGIETQAELLTIRDLGIACGQGYHIARPHANPKT 257 N+DPMKLQFVRSIQEIAEKSGSIVIAEGIE+QAELLTIRDLGIACGQGYHI+RP P Sbjct: 188 NHDPMKLQFVRSIQEIAEKSGSIVIAEGIESQAELLTIRDLGIACGQGYHISRPLPEPPV 247 Query: 258 VVADDISKILRRDKVPLTTQKYGPTKKSATVFKLLRESQYVAPDVSNDEVYNLFVNDPQL 317 ++DD++K LRRDKVPL +KYGP K S TVFKLLRE++YV+P+VSND VY++FVNDPQL Sbjct: 248 SISDDVTKTLRRDKVPLDAKKYGPGKHSTTVFKLLRETRYVSPEVSNDTVYDMFVNDPQL 307 Query: 318 QALPVVSNGIPVGLINRYSMVERFARLYGRELYGKKSCTFFMDPNPLLTEQDTSLQDLSH 377 QALPVVSNG+P+GLINRYSMVERFARLYGRELYGKKSC FFMDPNP+LTEQ TSLQDLS+ Sbjct: 308 QALPVVSNGLPIGLINRYSMVERFARLYGRELYGKKSCAFFMDPNPILTEQSTSLQDLSN 367 Query: 378 ILVQAESHHLSNGFIIVDQGQYVGMGTGHDLLREITQMQIEAARYANPLTQLPGNVPINE 437 +LV AESHHLSNGFII D+GQY+G+GTGHDLLREITQMQI+AAR+ANPLTQLPGNVPIN+ Sbjct: 368 LLVHAESHHLSNGFIITDRGQYMGLGTGHDLLREITQMQIDAARHANPLTQLPGNVPIND 427 Query: 438 HIEFLLQSGVEFCICYCDLDNFKPFNDVYGYRKGDDMIQLTGRTLSQFCDTQLDFIGHIG 497 HIE+LLQSGVEFCICYCDLD FKPFNDVYGYRKGDDMIQ TGR L Q CD +LDFIGHIG Sbjct: 428 HIEYLLQSGVEFCICYCDLDQFKPFNDVYGYRKGDDMIQATGRVLQQACDPELDFIGHIG 487 Query: 498 GDDFLIMFQSEDWEHRCNAMLDAFAVASQNFYYVEDKERGGYYSEDRQGNRVLHPLVSLS 557 GDDFLI+FQSEDWE RC +MLD F ++ FYY ED ERGGYY+EDRQGNRV H LVS+S Sbjct: 488 GDDFLILFQSEDWEKRCRSMLDNFDAETRGFYYDEDVERGGYYTEDRQGNRVFHKLVSVS 547 Query: 558 LGAVKIEPSQYSSPHQIASAATKAKKQAKKTPGNSLFIERRVPATFSWAM 607 LGAVKIEPSQYSSPHQIASAAT+AKKQAKK PGNSLFIERR+PATFSWA+ Sbjct: 548 LGAVKIEPSQYSSPHQIASAATRAKKQAKKIPGNSLFIERRIPATFSWAL 597 >gi|300313035|ref|YP_003777127.1| serine phosphatase, regulator of sigma subunit [Herbaspirillum seropedicae SmR1] Length = 422 Score = 101 bits (251), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 48/121 (39%), Positives = 77/121 (63%) Query: 301 DVSNDEVYNLFVNDPQLQALPVVSNGIPVGLINRYSMVERFARLYGRELYGKKSCTFFMD 360 D +N V +F +L +LPVV P+GLI+R+ + + ++ Y RELY +KSC FMD Sbjct: 33 DQNNAAVLEIFDQHKELVSLPVVEQQRPIGLISRHIFMSQMSKPYHRELYERKSCIAFMD 92 Query: 361 PNPLLTEQDTSLQDLSHILVQAESHHLSNGFIIVDQGQYVGMGTGHDLLREITQMQIEAA 420 +PL+ + DT ++ L + V + L++GF+IV+QGQ G+G+G L+RE+ +Q+E Sbjct: 93 KDPLIVDADTPIETLGALAVASGDKTLADGFMIVEQGQLNGLGSGLALMRELVDLQVEKN 152 Query: 421 R 421 R Sbjct: 153 R 153 >gi|300309883|ref|YP_003773975.1| GGDEF domain/EAL domain signal transduction protein [Herbaspirillum seropedicae SmR1] Length = 650 Score = 99.8 bits (247), Expect = 5e-22, Method: Compositional matrix adjust. Identities = 75/248 (30%), Positives = 112/248 (45%), Gaps = 22/248 (8%) Query: 28 QRSLTALFQPIIRMRSGEIIGYEGLIRGPSNSP-LHSPLNLFKVAR----ANKLSVQVEH 82 +R L+ FQPI+R+ G++I +E L R S SP+ A N+LSV + Sbjct: 407 ERELSVFFQPIVRLDDGQVIAFEALARWHSTVKGAVSPVEFIPAAERMGIINQLSVILLR 466 Query: 83 LCRHIVMAQFAQSGLPGNLF---LNVSPGALVQPEAIQGETLRHLEKLGINPQRIIIELT 139 + AQ L NL +N S G E ++ + + G P R+ +E+T Sbjct: 467 KALAAAASWPAQIRLSFNLSANDINSSEG--------MAEIIKLIRESGFCPSRLDLEIT 518 Query: 140 ENEPTYDYTLLRNAAMHYRDMGFQIAIDDLGEGFSSLRLWSELQPEYVKVDMHFIQGINN 199 E D R R +G I +DD G G++SL L +K+D F+ I Sbjct: 519 ETALLQDLEKTRQLIHSLRQLGCGITLDDFGTGYASLSHLHALPLTRIKIDKSFVADIGQ 578 Query: 200 DPMKLQFVRSIQEIAEKSGSIVIAEGIETQAELLTIRDLGIACGQGYHIARPHANPKTVV 259 D + L+ V+S+ + + EG+ET L +R+LG GQGY ARP A Sbjct: 579 DAVSLKIVKSLLALTLDMDLACVVEGLETPQHLAVLRELGAMYGQGYLYARPMA------ 632 Query: 260 ADDISKIL 267 ADD++ L Sbjct: 633 ADDVAPWL 640 >gi|300311038|ref|YP_003775130.1| hypothetical protein Hsero_1709 [Herbaspirillum seropedicae SmR1] Length = 598 Score = 92.0 bits (227), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 70/226 (30%), Positives = 111/226 (49%), Gaps = 15/226 (6%) Query: 35 FQPIIRMRSGEIIGYEGLIRGPSNSPLHS--PLNLF-----KVARANKLSVQVEHLCRHI 87 FQP +R+R G +G E LIR + P+H P ++F K + +++ V R + Sbjct: 365 FQPKVRLRDGACVGVEALIRW--HHPIHGNIPPDVFIPLAEKTGQIRQVTAWV---LRQV 419 Query: 88 V--MAQFAQSGLPGNLFLNVSPGALVQPEAIQGETLRHLEKLGINPQRIIIELTENEPTY 145 V +A++ +SG + N S E ++ + L+ + P I IE TE+ Sbjct: 420 VAQVARWLKSGRALPVAFNASARDFDSDE-LRELLAQQLKLHKVPPALIEIEFTEHAMAN 478 Query: 146 DYTLLRNAAMHYRDMGFQIAIDDLGEGFSSLRLWSELQPEYVKVDMHFIQGINNDPMKLQ 205 +R R++G ++AIDD G GFS+L + + +K+D FI + +D Sbjct: 479 HPQQVRQRVQALRELGVKVAIDDFGAGFSNLSYLKNIPADTMKIDRSFIHRLLDDEADQL 538 Query: 206 FVRSIQEIAEKSGSIVIAEGIETQAELLTIRDLGIACGQGYHIARP 251 V SI +A G V+AEG+E++ +R LG GQGY IARP Sbjct: 539 IVPSILHLAHNLGFSVVAEGLESERTATLLRHLGCDYGQGYAIARP 584 >gi|300312012|ref|YP_003776104.1| phosphodiesterase transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 797 Score = 84.0 bits (206), Expect = 3e-17, Method: Compositional matrix adjust. Identities = 63/236 (26%), Positives = 106/236 (44%), Gaps = 35/236 (14%) Query: 35 FQPIIRMRSGEIIGYEGLIR---------GPSN-------SPLHSPLNLFKVARANKLSV 78 QP + + SG+++G E L+R GP N + L L+ + + +LS Sbjct: 551 LQPKLELCSGQVVGAEALVRWIHPEKGFIGPDNFIPFAETTGFIRMLTLWMMEQVARLSE 610 Query: 79 QVEHLCRHIVMAQFAQSGLPGNLFLNVSPGALVQ---PEAIQGETLRHLEKLGINPQRII 135 Q++ Q G+ +N+S L+ P A RH + P + Sbjct: 611 QLQ------------QQGVQLKFSVNLSTRDLLDQELPTAFAAILQRH----RLQPAALC 654 Query: 136 IELTENEPTYDYTLLRNAAMHYRDMGFQIAIDDLGEGFSSLRLWSELQPEYVKVDMHFIQ 195 +E+TE+ D +N +MGF ++IDD G G+SSL L + +K+D F+ Sbjct: 655 LEITESAIMDDPARAQNTLERLSNMGFALSIDDFGTGYSSLAYLKRLPVDELKIDKSFVM 714 Query: 196 GINNDPMKLQFVRSIQEIAEKSGSIVIAEGIETQAELLTIRDLGIACGQGYHIARP 251 + D + VRS ++ G V+AEG+E++ +R +G GQGY + +P Sbjct: 715 KMAQDQDDAKIVRSTVDLGHNLGLRVVAEGVESEEVWFLLRQMGCDQGQGYFMGKP 770 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.137 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,882,989 Number of extensions: 78817 Number of successful extensions: 269 Number of sequences better than 1.0e-08: 21 Number of HSP's gapped: 261 Number of HSP's successfully gapped: 21 Length of query: 607 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 516 Effective length of database: 1,188,985 Effective search space: 613516260 Effective search space used: 613516260 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)