BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0204.0004.p (615 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311228|ref|YP_003775320.1| DNA translocase FtsK2 transmemb... 457 e-129 gi|300310771|ref|YP_003774863.1| DNA translocase FtsK 1 protein ... 252 7e-68 >gi|300311228|ref|YP_003775320.1| DNA translocase FtsK2 transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 780 Score = 457 bits (1176), Expect = e-129, Method: Compositional matrix adjust. Identities = 269/444 (60%), Positives = 300/444 (67%), Gaps = 71/444 (15%) Query: 237 SPGGVLGEMIGSAAQTSLGFTGATLLLLLLFGLGFSLFFHVSWLAAVERIGGAIEDGIFW 296 S GGVLGEMIGSA Q + GFTG+TLLLL+LFGLGFSL FHVSWLAAVERIGG IEDG+FW Sbjct: 156 SSGGVLGEMIGSAVQPTFGFTGSTLLLLMLFGLGFSLLFHVSWLAAVERIGGLIEDGLFW 215 Query: 297 IRNFYSAREDRKVGHVAAVKREEVVVQERAKIDEAPPIRIEPQVVAVPKSERIEKERQTS 356 +R+F++AR DRK G AAVKREE VVQERAKI EAPPIRIEPQ+V V KS+R++KE+QTS Sbjct: 216 VRDFFAARADRKAGQEAAVKREETVVQERAKIVEAPPIRIEPQIVEVQKSDRVQKEKQTS 275 Query: 357 LFQDIDSSLPPLSLLDEAPPAQQTVSVETLEFTSRLIEKKLSDFGVEVKVVAAYPGPVIT 416 LF D+ + LPPLSLLDEAPP+QQTVSVETLEFTSRLIEKKLSDFGVEVKVVAAYPGPVIT Sbjct: 276 LFDDVSTDLPPLSLLDEAPPSQQTVSVETLEFTSRLIEKKLSDFGVEVKVVAAYPGPVIT 335 Query: 417 RYEIEPATGVKGSQIVGLARDLGLPTGLIKANV----------------PTRIAFQVSSK 460 RYEIEPATGVKGSQIV LARDL L V P R +++ Sbjct: 336 RYEIEPATGVKGSQIVNLARDLARSLSLTSIRVVEVIQGKNYMGLELPNPKRQIVRLTEI 395 Query: 461 IDSRTILDQMSIYE-----------------GIPHLL--------------APVXS-IYE 488 + S+ D S +PHLL A + S +Y+ Sbjct: 396 LGSKVYNDSHSSLTVALGKDIAGNPVVADLAKMPHLLVAGTTGSGKSVGINATILSLLYK 455 Query: 489 GIPHLLAPVVTD----------------------MRQAGHALNWAVAEMERRYKRMSKLG 526 P + ++ D MRQAGHALNWAV EMERRYK+MSKLG Sbjct: 456 STPRQVRLILIDPKMLELSIYEGIPHLLAPVVTDMRQAGHALNWAVEEMERRYKKMSKLG 515 Query: 527 VRNLAGYNQKIIDADKRGEKIPNPFSLTPDAPEPLEQLETIVIIIDELADLMMVXRPDGR 586 VRNLAGYNQKI DA+KRGEKIPNPFSLTPDAPEPLEQLETIVIIIDELADLMMV Sbjct: 516 VRNLAGYNQKIADAEKRGEKIPNPFSLTPDAPEPLEQLETIVIIIDELADLMMVVGKKVE 575 Query: 587 GSAARHGRHAVHAAGHR-LADSRP 609 AR + A A H LA RP Sbjct: 576 ELIARIAQKARAAGIHLILATQRP 599 Score = 319 bits (818), Expect = 4e-88, Method: Compositional matrix adjust. Identities = 159/167 (95%), Positives = 163/167 (97%) Query: 75 ILGLARDLARSLSLTSIRVVEVIQGKNYMGLELPNPKRQIVRLTEILGSKVYNDSHSTLT 134 I+ LARDLARSLSLTSIRVVEVIQGKNYMGLELPNPKRQIVRLTEILGSKVYNDSHS+LT Sbjct: 350 IVNLARDLARSLSLTSIRVVEVIQGKNYMGLELPNPKRQIVRLTEILGSKVYNDSHSSLT 409 Query: 135 VALGKDIAGNPVVADLAKMPHLLVAGTTGSGKSVGINATILSLLYKSTPKQVRLILIDPK 194 VALGKDIAGNPVVADLAKMPHLLVAGTTGSGKSVGINATILSLLYKSTP+QVRLILIDPK Sbjct: 410 VALGKDIAGNPVVADLAKMPHLLVAGTTGSGKSVGINATILSLLYKSTPRQVRLILIDPK 469 Query: 195 MLELSIYEGIPHLLAPVVTDMRQAGHALNWAVAEMERRYKRMSPGGV 241 MLELSIYEGIPHLLAPVVTDMRQAGHALNWAV EMERRYK+MS GV Sbjct: 470 MLELSIYEGIPHLLAPVVTDMRQAGHALNWAVEEMERRYKKMSKLGV 516 Score = 148 bits (374), Expect = 1e-36, Method: Compositional matrix adjust. Identities = 74/76 (97%), Positives = 76/76 (100%) Query: 1 VRLTEILGSKVYNDSHSTLTVALGKDIAGNPVVADLAKMPHLLVAGTTGSGKSVGINATI 60 VRLTEILGSKVYNDSHS+LTVALGKDIAGNPVVADLAKMPHLLVAGTTGSGKSVGINATI Sbjct: 390 VRLTEILGSKVYNDSHSSLTVALGKDIAGNPVVADLAKMPHLLVAGTTGSGKSVGINATI 449 Query: 61 LSLLYKSTPKQVRLIL 76 LSLLYKSTP+QVRLIL Sbjct: 450 LSLLYKSTPRQVRLIL 465 Score = 60.8 bits (146), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 29/29 (100%), Positives = 29/29 (100%) Query: 442 TGLIKANVPTRIAFQVSSKIDSRTILDQM 470 TGLIKANVPTRIAFQVSSKIDSRTILDQM Sbjct: 605 TGLIKANVPTRIAFQVSSKIDSRTILDQM 633 >gi|300310771|ref|YP_003774863.1| DNA translocase FtsK 1 protein [Herbaspirillum seropedicae SmR1] Length = 524 Score = 252 bits (643), Expect = 7e-68, Method: Compositional matrix adjust. Identities = 121/167 (72%), Positives = 143/167 (85%) Query: 75 ILGLARDLARSLSLTSIRVVEVIQGKNYMGLELPNPKRQIVRLTEILGSKVYNDSHSTLT 134 ++ L +DL+R+L LTSIRVVE I GK MGLELPN +RQ+++L+EI+ S+ Y S S LT Sbjct: 96 VVNLMKDLSRALGLTSIRVVETIPGKTCMGLELPNARRQMIKLSEIVHSQAYRKSASHLT 155 Query: 135 VALGKDIAGNPVVADLAKMPHLLVAGTTGSGKSVGINATILSLLYKSTPKQVRLILIDPK 194 +A+GKDI G PVV DLA+ PH+LVAGTTGSGKSV INA ILSLLYK+TP++VRLI+IDPK Sbjct: 156 IAMGKDITGTPVVTDLARAPHMLVAGTTGSGKSVAINAMILSLLYKATPEEVRLIMIDPK 215 Query: 195 MLELSIYEGIPHLLAPVVTDMRQAGHALNWAVAEMERRYKRMSPGGV 241 MLELSIYEGIPHLLAPVVTDMR+A HALNWAV EMERRYK+MS GV Sbjct: 216 MLELSIYEGIPHLLAPVVTDMREAAHALNWAVDEMERRYKKMSKLGV 262 Score = 181 bits (458), Expect = 2e-46, Method: Compositional matrix adjust. Identities = 93/126 (73%), Positives = 100/126 (79%), Gaps = 1/126 (0%) Query: 485 SIYEGIPHLLAPVVTDMRQAGHALNWAVAEMERRYKRMSKLGVRNLAGYNQKIIDADKRG 544 SIYEGIPHLLAPVVTDMR+A HALNWAV EMERRYK+MSKLGVRNLAGYNQK+ +A RG Sbjct: 220 SIYEGIPHLLAPVVTDMREAAHALNWAVDEMERRYKKMSKLGVRNLAGYNQKLDEAAARG 279 Query: 545 EKIPNPFSLTPDAPEPLEQLETIVIIIDELADLMMVXRPDGRGSAARHGRHAVHAAGHR- 603 EKIPNPFSLTPDAPEPLE+L TIVI+IDELADLMMV AR + A A H Sbjct: 280 EKIPNPFSLTPDAPEPLEKLPTIVIVIDELADLMMVVGKKVEELIARLAQKARAAGIHLI 339 Query: 604 LADSRP 609 LA RP Sbjct: 340 LATQRP 345 Score = 108 bits (270), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 51/76 (67%), Positives = 64/76 (84%) Query: 1 VRLTEILGSKVYNDSHSTLTVALGKDIAGNPVVADLAKMPHLLVAGTTGSGKSVGINATI 60 ++L+EI+ S+ Y S S LT+A+GKDI G PVV DLA+ PH+LVAGTTGSGKSV INA I Sbjct: 136 IKLSEIVHSQAYRKSASHLTIAMGKDITGTPVVTDLARAPHMLVAGTTGSGKSVAINAMI 195 Query: 61 LSLLYKSTPKQVRLIL 76 LSLLYK+TP++VRLI+ Sbjct: 196 LSLLYKATPEEVRLIM 211 Score = 87.0 bits (214), Expect = 4e-18, Method: Compositional matrix adjust. Identities = 65/181 (35%), Positives = 91/181 (50%), Gaps = 12/181 (6%) Query: 333 PIRIEPQVVAVPKSERIEKERQTSLFQDIDSSLPPLSLLDEAPPAQQTVSVETLEFTSRL 392 P EP V++ + +++ R ++ LP L LLDE P ++ V E L+ T RL Sbjct: 5 PTAPEPAPVSMAYPDYVDEPR-------VEIPLPSLDLLDEVPESRIEVDTEQLQETGRL 57 Query: 393 IEKKLSDFGVEVKVVAAYPGPVITRYEIEPATGVKGSQIVGLARDLGLPTGLIKANVPTR 452 IE++L +F V V V+ A GPVITR+E+EPA GV+G+Q+V L +DL GL V Sbjct: 58 IEQRLKEFKVPVTVLGAEAGPVITRFEVEPAQGVRGAQVVNLMKDLSRALGLTSIRVVET 117 Query: 453 IAFQVSSKIDSRTILDQMSIYEGIPHLLAPVXS-----IYEGIPHLLAPVVTDMRQAGHA 507 I + ++ QM I H A S I G PVVTD+ +A H Sbjct: 118 IPGKTCMGLELPNARRQMIKLSEIVHSQAYRKSASHLTIAMGKDITGTPVVTDLARAPHM 177 Query: 508 L 508 L Sbjct: 178 L 178 Score = 59.7 bits (143), Expect = 7e-10, Method: Compositional matrix adjust. Identities = 26/29 (89%), Positives = 29/29 (100%) Query: 442 TGLIKANVPTRIAFQVSSKIDSRTILDQM 470 TGLIKAN+PTR+AFQVSSKIDSRT+LDQM Sbjct: 351 TGLIKANIPTRVAFQVSSKIDSRTVLDQM 379 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,720,603 Number of extensions: 69989 Number of successful extensions: 329 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 327 Number of HSP's successfully gapped: 10 Length of query: 615 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 524 Effective length of database: 1,188,985 Effective search space: 623028140 Effective search space used: 623028140 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)