BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0202.0002.p (338 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310936|ref|YP_003775028.1| transcription regulator protein... 217 1e-57 gi|300310937|ref|YP_003775029.1| heavy metal translocating P-typ... 97 1e-21 gi|300310933|ref|YP_003775025.1| metal-transporting P-type ATPas... 71 1e-13 gi|300310934|ref|YP_003775026.1| Pb-specific transcription regul... 64 2e-11 >gi|300310936|ref|YP_003775028.1| transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 141 Score = 217 bits (552), Expect = 1e-57, Method: Compositional matrix adjust. Identities = 99/129 (76%), Positives = 113/129 (87%) Query: 204 MNIGEAAQASGVSAKMIRHYEDIGLVPKAARTDAGYRIYGERDVHLLRFIRQGRVLGFSM 263 MNIGEAA ASGVSAKMIRHYE+ GL+P+A+RTDAGYR+YG+RDVHLLRFIRQ R LGFSM Sbjct: 1 MNIGEAASASGVSAKMIRHYEETGLIPRASRTDAGYRVYGQRDVHLLRFIRQARQLGFSM 60 Query: 264 KQIGDLMGLWLDQSRPSRKVKQLANDHIAELDVKIRELQAMKSTLQQLAHDCHGDNRPDC 323 Q+ DL+GLWLDQSRPSRKVKQLA HIAEL+ +I ELQ MK+TL+QL CHGDNRPDC Sbjct: 61 AQVSDLIGLWLDQSRPSRKVKQLAQTHIAELEQRISELQGMKATLEQLVQGCHGDNRPDC 120 Query: 324 PILDALSAE 332 PIL+AL + Sbjct: 121 PILEALGGD 129 >gi|300310937|ref|YP_003775029.1| heavy metal translocating P-type ATPase [Herbaspirillum seropedicae SmR1] Length = 744 Score = 97.4 bits (241), Expect = 1e-21, Method: Compositional matrix adjust. Identities = 96/268 (35%), Positives = 125/268 (46%), Gaps = 48/268 (17%) Query: 19 QRNSEHPLAHAVMAAAAQRHLTPAAADDSKAVPGRGTQALIGNRLSYLAQELGATPDQDX 78 Q +SEHPL AV AAAQ A+ ++V G G + ++ R L Sbjct: 461 QAHSEHPLGRAVREAAAQSAQAARQAEQVRSVAGLGIEGVVEGRQLMLG---------SS 511 Query: 79 RWMQELGATPDQHA-ALAGIAKTLEQQGRTISWLAQSEADGGQVALLGLLAFGDTLKNSA 137 RWMQ+L A HA AL A+ L+ QGRT+SWLA+ G V +LGLLAFGD +K A Sbjct: 512 RWMQQLQA----HAPALQARAEQLQAQGRTLSWLARRNDSG--VEVLGLLAFGDQIKPGA 565 Query: 138 QQAVNSLHALGVRTVMLSGDNYGAAAHVAXRRGDGDIE---------------RERGQQC 182 Q A+ +LH +GVR+ MLSGDN GAA VA G ++ R+ G Q Sbjct: 566 QAAIATLHRMGVRSAMLSGDNLGAAQQVASELGIQEVRAEVLPADKAEAVTALRQGGAQV 625 Query: 183 TAAQALETKGVPAIPGS-TDMTMNIGE--AAQASGVSAKMIRHYEDIGLVPKAARTDAGY 239 A PA+ + M M+ G A QA+GV+ + D GLV A D Sbjct: 626 VAMVGDGVNDAPALAAADVGMAMSTGTDVAMQAAGVTLM----HGDPGLVADA--IDISR 679 Query: 240 RIYGERDVHLLRFIRQGRVLGFSMKQIG 267 R Y + IRQ F +G Sbjct: 680 RTYAK--------IRQNLFWAFIYNLVG 699 >gi|300310933|ref|YP_003775025.1| metal-transporting P-type ATPase transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 778 Score = 71.2 bits (173), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 61/176 (34%), Positives = 88/176 (50%), Gaps = 26/176 (14%) Query: 7 DADRLLALAAALQRNSEHPLAHAVMAAAAQRHLTPAAADDSKAVPGRGTQALIGNRLSYL 66 D LL LA +L S+HP++ A+ AAQ + + A+ GRG Q I +L +L Sbjct: 490 DQQALLQLAVSLAARSDHPVSRAI---AAQEEIATVEVSEFAALAGRGVQGRINGQLLHL 546 Query: 67 AQELGATPDQDXRWMQELG-ATPDQHAALAGIAKTLEQQGRTISWLAQSEADGGQVALLG 125 + R + ELG +P+ A L + E+QG+T + L GQ LL Sbjct: 547 G---------NHRLVHELGQCSPELEARL----QAFEKQGKTTTVLCLD----GQPQLL- 588 Query: 126 LLAFGDTLKNSAQQAVNSLHALGVRTVMLSGDNYGAAAHVAXRRGDGDIERERGQQ 181 +A DT++ S++QAV L ALGV +ML+GDN A +A + G D RG Q Sbjct: 589 -VAVADTVRPSSRQAVAELRALGVEVIMLTGDNSHTAQAIAAQTGISD---ARGDQ 640 >gi|300310934|ref|YP_003775026.1| Pb-specific transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 150 Score = 63.5 bits (153), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 32/96 (33%), Positives = 52/96 (54%) Query: 203 TMNIGEAAQASGVSAKMIRHYEDIGLVPKAARTDAGYRIYGERDVHLLRFIRQGRVLGFS 262 T+ IGE A + + +R+YE L+P+ R+ A YR+YGER V L+FIR R L + Sbjct: 3 TLKIGELAAKADCPVETVRYYEKEALLPEPTRSQANYRLYGERHVERLQFIRHCRSLDMT 62 Query: 263 MKQIGDLMGLWLDQSRPSRKVKQLANDHIAELDVKI 298 + ++ +L+ L + V L + HI + +I Sbjct: 63 LDEVRELLRLRDAPEQDCGGVNALLDKHIGHVAERI 98 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.131 0.378 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 997,526 Number of extensions: 40385 Number of successful extensions: 173 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 171 Number of HSP's successfully gapped: 6 Length of query: 338 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 252 Effective length of database: 1,212,660 Effective search space: 305590320 Effective search space used: 305590320 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)