BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0201.0024.p (113 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309741|ref|YP_003773833.1| periplasmic protein TonB, links... 182 4e-48 >gi|300309741|ref|YP_003773833.1| periplasmic protein TonB, links inner and outer membranes [Herbaspirillum seropedicae SmR1] Length = 307 Score = 182 bits (463), Expect = 4e-48, Method: Compositional matrix adjust. Identities = 84/110 (76%), Positives = 97/110 (88%), Gaps = 1/110 (0%) Query: 2 YYKALQEKIEKIGTLNFPQKDGKKLYGELVIYIPVYQDGSIYDKEGGVRVERSSGNLALD 61 YYK LQ+KIEK+GTLNFP KDGKKLYGEL+I IPV+QDGSIY+++GGV V+ SSGN ALD Sbjct: 198 YYKTLQDKIEKVGTLNFPTKDGKKLYGELLIVIPVFQDGSIYERDGGVVVKSSSGNAALD 257 Query: 62 AAALTIVRRSAPFGRFPDNMRTTGGKDDIWEVITRFRFTRDETLQTDLSG 111 AA+ IVRRSAPFGRFPDNMR TGG+DD+WE+ITRFRFTRD+ LQ L G Sbjct: 258 QAAVAIVRRSAPFGRFPDNMR-TGGRDDVWEIITRFRFTRDQGLQAQLGG 306 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.138 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 361,246 Number of extensions: 13987 Number of successful extensions: 32 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 31 Number of HSP's successfully gapped: 1 Length of query: 113 Length of database: 1,619,870 Length adjustment: 73 Effective length of query: 40 Effective length of database: 1,274,215 Effective search space: 50968600 Effective search space used: 50968600 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 124 (52.4 bits)