BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0201.0014.p (181 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309729|ref|YP_003773821.1| biotin carboxylase [Herbaspiril... 317 3e-88 gi|300313399|ref|YP_003777491.1| acyl-CoA carboxylase biotin sub... 150 7e-38 gi|300313973|ref|YP_003778065.1| acetyl/propionyl-CoA carboxylas... 147 6e-37 gi|300313753|ref|YP_003777845.1| urea amidolyase [Herbaspirillum... 129 1e-31 >gi|300309729|ref|YP_003773821.1| biotin carboxylase [Herbaspirillum seropedicae SmR1] Length = 459 Score = 317 bits (813), Expect = 3e-88, Method: Compositional matrix adjust. Identities = 157/161 (97%), Positives = 159/161 (98%) Query: 1 MFEKILIANRGEIALRIQRACREMGIKTVVVHSEADREAKYVKLADESVCIGPAPSALSY 60 MFEKILIANRGEIALRIQRACRE+GIKTVVVHSEADREAKYVKLADESVCIGPAPS LSY Sbjct: 1 MFEKILIANRGEIALRIQRACRELGIKTVVVHSEADREAKYVKLADESVCIGPAPSTLSY 60 Query: 61 LNMPAIISAAEVTDAQAIHPGYGFLSENADFAERVEKSGFVFIGPRPENIRMMGDKVSAK 120 LNMPAIISAAEVTDAQAIHPGYGFLSENADFAERVEKSGFVFIGPR ENIRMMGDKVSAK Sbjct: 61 LNMPAIISAAEVTDAQAIHPGYGFLSENADFAERVEKSGFVFIGPRAENIRMMGDKVSAK 120 Query: 121 QAMIRAGVPCVPGSEGALPDNPKEIVQIARKIGYPVIIKAA 161 QAMIRAGVPCVPGS+GALPDNPKEIVQIARKIGYPVIIKAA Sbjct: 121 QAMIRAGVPCVPGSDGALPDNPKEIVQIARKIGYPVIIKAA 161 >gi|300313399|ref|YP_003777491.1| acyl-CoA carboxylase biotin subunit [Herbaspirillum seropedicae SmR1] Length = 462 Score = 150 bits (379), Expect = 7e-38, Method: Compositional matrix adjust. Identities = 86/165 (52%), Positives = 113/165 (68%), Gaps = 1/165 (0%) Query: 2 FEKILIANRGEIALRIQRACREMGIKTVVVHSEADREAKYVKLADESVCIGPAPSALSYL 61 K+LIANRGEIA+RI RA + +GI TV S+AD ++ ++ADE IGPA + SYL Sbjct: 5 LRKLLIANRGEIAVRIIRAAQALGITTVAACSDADTDSLAARMADEVQRIGPARADRSYL 64 Query: 62 NMPAIISAAEVTDAQAIHPGYGFLSENADFAERVEKSGFVFIGPRPENIRMMGDKVSAKQ 121 N+ A++ AA + A A+HPGYGFLSENA FAE V +G +F+GP+ + IR MGDK A++ Sbjct: 65 NVDALLKAARDSGADALHPGYGFLSENAAFAEAVNAAGLIFVGPQADTIRRMGDKAEARR 124 Query: 122 AMIRAGVPCVPGSEGALPDNPKEIVQIARKIGYPVIIKAAGGGGG 166 AGVP VPGS G L D + A +IGYP++IKA+ GGGG Sbjct: 125 TAAAAGVPVVPGSAGELEDLATALA-CADEIGYPLLIKASAGGGG 168 >gi|300313973|ref|YP_003778065.1| acetyl/propionyl-CoA carboxylase subunit alpha [Herbaspirillum seropedicae SmR1] Length = 671 Score = 147 bits (370), Expect = 6e-37, Method: Compositional matrix adjust. Identities = 88/161 (54%), Positives = 107/161 (66%), Gaps = 1/161 (0%) Query: 1 MFEKILIANRGEIALRIQRACREMGIKTVVVHSEADREAKYVKLADESVCIGPAPSALSY 60 MF KILIANRGEIA R+ R +GI TV V+S+AD AK+V DESV +GPAP+ SY Sbjct: 1 MFTKILIANRGEIACRVAATARRLGIATVAVYSDADARAKHVAACDESVRLGPAPARESY 60 Query: 61 LNMPAIISAAEVTDAQAIHPGYGFLSENADFAERVEKSGFVFIGPRPENIRMMGDKVSAK 120 L II+AA T AQAIHPGYGFLSENA FA+ ++G VFIGP I MG K +AK Sbjct: 61 LCAEKIIAAALATGAQAIHPGYGFLSENAGFAQACAQAGLVFIGPPASAIEAMGSKAAAK 120 Query: 121 QAMIRAGVPCVPGSEGALPDNPKEIVQIARKIGYPVIIKAA 161 M +A VP VPG G ++ Q A +IGYPV++KA+ Sbjct: 121 ALMEQAAVPLVPGYHGEEQESGLLHSQ-ADRIGYPVLLKAS 160 >gi|300313753|ref|YP_003777845.1| urea amidolyase [Herbaspirillum seropedicae SmR1] Length = 1207 Score = 129 bits (324), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 74/166 (44%), Positives = 108/166 (65%), Gaps = 2/166 (1%) Query: 1 MFEKILIANRGEIALRIQRACREMGIKTVVVHSEADREAKYVKLADESVCIGPAPSALSY 60 MF+ +LIANRG IA RI R R + + V V+SEAD + +V AD ++ +G P+A +Y Sbjct: 1 MFDHLLIANRGAIACRILRTLRTLDVTGVCVYSEADLGSLHVLQADRAISLGEGPAAQTY 60 Query: 61 LNMPAIISAAEVTDAQAIHPGYGFLSENADFAERVEKSGFVFIGPRPENIRMMGDKVSAK 120 L + I++AA + AQAIHPGYGFLSENA FAE E +G F+GP P +R+ G K +A+ Sbjct: 61 LAVDKILAAARDSGAQAIHPGYGFLSENAAFAEACEAAGIAFVGPTPAQLRIFGLKHTAR 120 Query: 121 QAMIRAGVPCVPGSEGALPDNPKEIVQIARKIGYPVIIKAAGGGGG 166 GVP + G++ L ++ + + A ++GYPV++K+ GGGG Sbjct: 121 ALAREQGVPMLEGTD--LLESIDDALAAASRVGYPVMLKSTAGGGG 164 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.136 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 579,581 Number of extensions: 23243 Number of successful extensions: 97 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 93 Number of HSP's successfully gapped: 4 Length of query: 181 Length of database: 1,619,870 Length adjustment: 79 Effective length of query: 102 Effective length of database: 1,245,805 Effective search space: 127072110 Effective search space used: 127072110 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 127 (53.5 bits)