BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0201.0010.p (419 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309727|ref|YP_003773819.1| transmembrane protein [Herbaspi... 176 4e-45 >gi|300309727|ref|YP_003773819.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 429 Score = 176 bits (445), Expect = 4e-45, Method: Compositional matrix adjust. Identities = 89/180 (49%), Positives = 126/180 (70%) Query: 239 EEAGRKKLADAEEEEVEEDEAGSDEDDENDEDAPAFVQKAERRERLGRIARVFMIVGAVV 298 E+ + + E +++ + + +D E+D + P FV++ ERRER GR+ +V M++ A V Sbjct: 224 EDVHHLRGHEDEPDQLSAADHSAADDSEDDAELPGFVRRVERRERAGRVLKVIMMILAGV 283 Query: 299 LGIALLLQATYVWRNTIAAWLPSTRPLLAAACAPLHCSVGLPTSIDQLVLESSELQLVPP 358 + LLLQA Y WRN +A LP RP+L C LHCSVGLP ID+L LE++ELQ+VPP Sbjct: 284 MVPVLLLQALYYWRNPLAEALPPVRPMLNGMCVALHCSVGLPAEIDRLSLEANELQVVPP 343 Query: 359 NQNIYTLSALLRNRSYGAQAWPYLELTLNDGDEKAITRRVFTPREYLGSAKLVXTCLAAN 418 NQN+Y L+ ++RNR GAQAWP+LELTLN+ DEKA+ RRVF PR+YL +A+ + +AA+ Sbjct: 344 NQNVYALTVVMRNRGPGAQAWPHLELTLNNDDEKAVVRRVFRPRDYLPNARQIDRGIAAD 403 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.312 0.127 0.378 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 811,806 Number of extensions: 22276 Number of successful extensions: 161 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 162 Number of HSP's successfully gapped: 4 Length of query: 419 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 331 Effective length of database: 1,203,190 Effective search space: 398255890 Effective search space used: 398255890 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 132 (55.5 bits)