BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0200.0009.p (264 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311040|ref|YP_003775132.1| major facilitator superfamily p... 352 2e-98 gi|300310856|ref|YP_003774948.1| major facilitator superfamily p... 254 8e-69 gi|300313188|ref|YP_003777280.1| major facilitator superfamily p... 134 7e-33 gi|300312683|ref|YP_003776775.1| major facilitator superfamily p... 103 2e-23 >gi|300311040|ref|YP_003775132.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 441 Score = 352 bits (904), Expect = 2e-98, Method: Compositional matrix adjust. Identities = 169/238 (71%), Positives = 207/238 (86%) Query: 1 MKGFLLTACGGGVAMLGFSLIGNHLVPQAWPIIMCVAGSVALFFYFRHAMRTEYPLLDLR 60 +KGF+LT+ G + MLG SL G+HLV A P+ MC G++AL Y+RH+ + E+PLLD R Sbjct: 175 VKGFVLTSVGAAMIMLGLSLFGSHLVDGATPVAMCSIGAIALVMYYRHSTQIEFPLLDFR 234 Query: 61 LLKIPTLRASVLGGSLFRVGLGAVPFLLPLALQEGLGLNPFEAGTITCASAFGAMFMKAL 120 LL+IPTL ASV+GGSLFR+GLGAVPFLLPLALQEGLG+ F+AG+ITCASAFG++F++ L Sbjct: 235 LLRIPTLHASVVGGSLFRIGLGAVPFLLPLALQEGLGMTAFQAGSITCASAFGSLFIRLL 294 Query: 121 ASMVLRRYGFRTVLMGNAVLAGMAIASYGIFSSHTPYYVMLLVVLLGGFFPSLQFTCLNS 180 A VLRRYGFRTVLM NAV AG+A+A+YGIFS HTPY +++ +VL+GG FP+LQFTCLNS Sbjct: 295 AGRVLRRYGFRTVLMVNAVFAGIALAAYGIFSIHTPYLLIVSIVLMGGVFPALQFTCLNS 354 Query: 181 IAYADISNRDAGRATSLASVVQQLSLGMGVTIAGLVLQISNYLQGHATIVEADFWPAF 238 IAYADI +RDAGR TSLASV+QQLSLG+GVT+AG+VLQ+SN+LQGHATIVEADFWPAF Sbjct: 355 IAYADIDSRDAGRVTSLASVIQQLSLGLGVTVAGIVLQLSNHLQGHATIVEADFWPAF 412 >gi|300310856|ref|YP_003774948.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 446 Score = 254 bits (648), Expect = 8e-69, Method: Compositional matrix adjust. Identities = 131/238 (55%), Positives = 167/238 (70%) Query: 1 MKGFLLTACGGGVAMLGFSLIGNHLVPQAWPIIMCVAGSVALFFYFRHAMRTEYPLLDLR 60 +KGFLL+A G + M+G + G HLV + +AG V L Y HA R PL+DL Sbjct: 175 LKGFLLSALGCSLLMMGLATAGRHLVSGEVALTCVLAGLVFLAAYVWHARRASAPLIDLA 234 Query: 61 LLKIPTLRASVLGGSLFRVGLGAVPFLLPLALQEGLGLNPFEAGTITCASAFGAMFMKAL 120 LLKIPTLRA V GGS +RVG+GA+PFLLPL LQ G G PF +G +TCASA GAMFMK + Sbjct: 235 LLKIPTLRAGVFGGSFYRVGIGAIPFLLPLMLQLGFGYTPFHSGMLTCASAAGAMFMKTI 294 Query: 121 ASMVLRRYGFRTVLMGNAVLAGMAIASYGIFSSHTPYYVMLLVVLLGGFFPSLQFTCLNS 180 A+ LRRYGFR VL+ N L + + + F+ TPY +M++++L+GG F SLQFT LN+ Sbjct: 295 AARFLRRYGFRRVLLVNGALGVLGLWAIATFTPQTPYLLMVVILLIGGCFRSLQFTSLNA 354 Query: 181 IAYADISNRDAGRATSLASVVQQLSLGMGVTIAGLVLQISNYLQGHATIVEADFWPAF 238 I+YAD+ +ATS+ASV QQLS+GMGV +AG LQ+SN +QGHAT+V DFWPAF Sbjct: 355 ISYADVDKLRLSQATSMASVAQQLSIGMGVVLAGFALQMSNEIQGHATLVAQDFWPAF 412 >gi|300313188|ref|YP_003777280.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 498 Score = 134 bits (337), Expect = 7e-33, Method: Compositional matrix adjust. Identities = 74/165 (44%), Positives = 110/165 (66%) Query: 46 FRHAMRTEYPLLDLRLLKIPTLRASVLGGSLFRVGLGAVPFLLPLALQEGLGLNPFEAGT 105 +RH R E+PLLD L+IPT +VL GSL R+G+ AVP+L+PL Q G GL F++G Sbjct: 264 WRHMQRIEHPLLDFSNLRIPTYSVTVLTGSLTRIGVDAVPYLMPLMFQIGFGLTAFQSGL 323 Query: 106 ITCASAFGAMFMKALASMVLRRYGFRTVLMGNAVLAGMAIASYGIFSSHTPYYVMLLVVL 165 + A+A G + MKA + +L+R+GFR+V + NA +AG I + G+ + TP V+L V+ Sbjct: 324 LLLATALGNLGMKAFTTRILQRFGFRSVAVNNAAIAGGLIIACGLLTPATPLPVILGVIF 383 Query: 166 LGGFFPSLQFTCLNSIAYADISNRDAGRATSLASVVQQLSLGMGV 210 G S+QF+ L ++AYADIS+ A++L SV QQ+++GMG+ Sbjct: 384 CYGLSRSMQFSTLATLAYADISDARKSSASTLWSVAQQMTIGMGI 428 >gi|300312683|ref|YP_003776775.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 498 Score = 103 bits (256), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 76/229 (33%), Positives = 116/229 (50%), Gaps = 12/229 (5%) Query: 5 LLTACGGGVAMLGFSLIGNHLVP-----QAWPIIMCVAGSVALFFYFRHAMRTEYPLLDL 59 LL+AC M+ FSL + VP W I + V ++ + Y+RHA PL L Sbjct: 246 LLSAC-----MVAFSLALD--VPFDSHRTGWSIALFVVAALTVLGYWRHASVHPRPLFRL 298 Query: 60 RLLKIPTLRASVLGGSLFRVGLGAVPFLLPLALQEGLGLNPFEAGTITCASAFGAMFMKA 119 L P + G + R+G AVPFLLPL LQ LG +P AG + +A K Sbjct: 299 GLFGEPNFSIGLAGNLVCRIGSSAVPFLLPLLLQVRLGYSPLHAGMVLLPAAIAGTIAKP 358 Query: 120 LASMVLRRYGFRTVLMGNAVLAGMAIASYGIFSSHTPYYVMLLVVLLGGFFPSLQFTCLN 179 + +++R+G+ L+ N + G AI S+ + + P ++ + + G S+QF +N Sbjct: 359 WIAPLVKRFGYDRFLLVNTAIVGAAIMSFALITPDWPLTAAIVQLAIFGASNSMQFAAMN 418 Query: 180 SIAYADISNRDAGRATSLASVVQQLSLGMGVTIAGLVLQISNYLQGHAT 228 S+ + RDAG SL S+VQ L++G+GVTI G ++ + G AT Sbjct: 419 SVTLKGLDKRDAGSGNSLFSMVQMLAIGLGVTIGGTMVNLFKSEVGTAT 467 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.329 0.142 0.432 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 745,641 Number of extensions: 27364 Number of successful extensions: 153 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 154 Number of HSP's successfully gapped: 4 Length of query: 264 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 181 Effective length of database: 1,226,865 Effective search space: 222062565 Effective search space used: 222062565 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 130 (54.7 bits)