BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0200.0003.p (346 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310272|ref|YP_003774364.1| 2-isopropylmalate synthase [Her... 268 3e-73 gi|300312728|ref|YP_003776820.1| 2-isopropylmalate synthase [Her... 228 8e-61 >gi|300310272|ref|YP_003774364.1| 2-isopropylmalate synthase [Herbaspirillum seropedicae SmR1] Length = 575 Score = 268 bits (686), Expect = 3e-73, Method: Compositional matrix adjust. Identities = 123/177 (69%), Positives = 144/177 (81%) Query: 1 MIAHPHTKYRALPAIDLPDRRWPSRTIEKAPVWLSTDLRDGNQALFEPMNSERKLTLFHE 60 M+ P KYRA+ +DLPDRRWPSRT+ +AP WLSTDLRDGNQALFEPMN ERKL LFHE Sbjct: 1 MLTDPSQKYRAIAPVDLPDRRWPSRTLRQAPTWLSTDLRDGNQALFEPMNRERKLRLFHE 60 Query: 61 LVRLGFKEIEVGFPAGSQTDFDVVRTLITGRHIPADVTPMVMTQLREEQIVRTIESLQGA 120 LVR+GFKEIEVGFPA S+ DF+ VR LI IP DVTPMVMTQLR + I T++S+ GA Sbjct: 61 LVRIGFKEIEVGFPAASRIDFETVRHLIDEHLIPDDVTPMVMTQLRADLITETVKSVAGA 120 Query: 121 PRAIVHFYNAVAPLWREVVFGLSVPQVMQLIERNVRLLKDLTAQHPETEWILQYSPE 177 R IVHFYNA+AP WRE+VFG+SVPQ++ L+E ++ L + LTA HPETEWILQYSPE Sbjct: 121 RRVIVHFYNAIAPAWREIVFGMSVPQIITLVEDHIALFRRLTAVHPETEWILQYSPE 177 Score = 165 bits (417), Expect = 6e-42, Method: Compositional matrix adjust. Identities = 113/236 (47%), Positives = 133/236 (56%), Gaps = 58/236 (24%) Query: 157 LLKDLTAQHPETEWILQYSP-------------------EXKGGIAFLLERDHGIVMPRR 197 + K AQ P+ W + Y P KGGIAFLL+RDH I MPRR Sbjct: 342 IAKGFAAQRPDAPWRVPYLPIDPTDLGRTYDSIVRVNSQSGKGGIAFLLQRDHHITMPRR 401 Query: 198 MQVEFSAIVQKHADASETEISSAALWNLFERTY---------------LASSTQNGRVRY 242 MQVEFSAIVQ ADASETE++S LW++FERTY LA + + R Y Sbjct: 402 MQVEFSAIVQALADASETELTSEHLWDVFERTYLAPVQASPDFSDRTSLAPALEQRRFIY 461 Query: 243 LSHQL-SNPDATQEILLELA-VDGIAIHLNGTGNGPIAATVNALRSGLAGLAGLEGLRID 300 SH L +PD + I+LELA +G + G+GNGPIAATV AL GLA LRID Sbjct: 462 RSHCLHPHPDG-ERIVLELADTEGNVRTVEGSGNGPIAATVAAL--GLA-------LRID 511 Query: 301 SYEERS--IGSGAQAQAFX----------GTRFGAARHVNIVTASILAVLSAANRL 344 SYEERS +G GA AQA G+RFG RH NI TASI+AVLSAA+R Sbjct: 512 SYEERSLGVGVGADAQALAIVEAALPTVPGSRFGVGRHENITTASIMAVLSAASRF 567 >gi|300312728|ref|YP_003776820.1| 2-isopropylmalate synthase [Herbaspirillum seropedicae SmR1] Length = 567 Score = 228 bits (580), Expect = 8e-61, Method: Compositional matrix adjust. Identities = 105/177 (59%), Positives = 130/177 (73%) Query: 1 MIAHPHTKYRALPAIDLPDRRWPSRTIEKAPVWLSTDLRDGNQALFEPMNSERKLTLFHE 60 M+++P KYR PAIDLPDR WPS+ I P+W+STDLRDGNQAL EPMN+ERKL F Sbjct: 2 MLSNPAAKYRPFPAIDLPDRTWPSKVINTPPIWMSTDLRDGNQALIEPMNAERKLRFFEL 61 Query: 61 LVRLGFKEIEVGFPAGSQTDFDVVRTLITGRHIPADVTPMVMTQLREEQIVRTIESLQGA 120 L++ G KEIEVGFP+ SQTDFD VR LI IP DVT +V+TQ REE I RTIESL+GA Sbjct: 62 LLKTGLKEIEVGFPSASQTDFDFVRKLIVENRIPDDVTIIVLTQSREELIRRTIESLEGA 121 Query: 121 PRAIVHFYNAVAPLWREVVFGLSVPQVMQLIERNVRLLKDLTAQHPETEWILQYSPE 177 +AIVH YN+VAP +R++VF +S ++ + RL+K+LT P TEW +YSPE Sbjct: 122 KKAIVHLYNSVAPAFRKIVFNMSREEIKNIAVTGTRLVKELTDARPGTEWRFEYSPE 178 Score = 106 bits (264), Expect = 3e-24, Method: Compositional matrix adjust. Identities = 74/219 (33%), Positives = 114/219 (52%), Gaps = 49/219 (22%) Query: 159 KDLTAQHPETEWILQYSP-------------------EXKGGIAFLLERDHGIVMPRRMQ 199 K Q P+ W + Y P KGG+A+LLE+++G+ +PRR+Q Sbjct: 345 KGFAKQQPDAIWEVPYLPIDPADLGRSYDAVIRVNSQSGKGGMAYLLEQEYGLALPRRLQ 404 Query: 200 VEFSAIVQKHADASETEISSAALWNLFERTYLASSTQNGRVR---YLSHQLSNPDATQE- 255 +EFS +Q+ ADA+ EI+++ + +F+R YL RV Y +H++S + E Sbjct: 405 IEFSRAIQREADATGKEIAASDIHAIFQREYLE------RVEPYVYRAHRMSEDSSKAES 458 Query: 256 --ILLELAVDGIAIHLNGTGNGPIAATVNALRSGLAGLAGLEGLRIDSYEERSIGSGAQA 313 I +++ +G + + G+GNGPI A V+AL GL+ +++ + E +IG+GA A Sbjct: 459 INIEVDIVRNGQPVTVRGSGNGPIDAFVHAL--------GLD-IKLMDFHEHAIGAGADA 509 Query: 314 QAFX---------GTRFGAARHVNIVTASILAVLSAANR 343 +A T FG NIVTAS AVLSA NR Sbjct: 510 KAASYIELRLNEAPTGFGVGIDANIVTASFKAVLSAVNR 548 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.134 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,032,422 Number of extensions: 40771 Number of successful extensions: 126 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 118 Number of HSP's successfully gapped: 4 Length of query: 346 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 260 Effective length of database: 1,212,660 Effective search space: 315291600 Effective search space used: 315291600 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)