BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0199.0010.p
         (323 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300312596|ref|YP_003776688.1| 3-isopropylmalate dehydratase l...   350   7e-98
gi|300311598|ref|YP_003775690.1| aconitate hydratase [Herbaspiri...    75   7e-15
gi|300312283|ref|YP_003776375.1| aconitate hydratase [Herbaspiri...    69   6e-13

>gi|300312596|ref|YP_003776688.1| 3-isopropylmalate dehydratase
           large subunit [Herbaspirillum seropedicae SmR1]
          Length = 466

 Score =  350 bits (899), Expect = 7e-98,   Method: Compositional matrix adjust.
 Identities = 160/283 (56%), Positives = 204/283 (72%)

Query: 1   LQALEENCSQFDLTYFSLNDPRQGILHVVAPELGDTLPGTTIACGDSHTTTHGAFAALAF 60
           ++ L+ N  ++ LTYF +ND RQGI+HV+ PE G TLPG T+ CGDSHT+THGAFAALA 
Sbjct: 83  VETLDGNAKEYSLTYFGMNDKRQGIVHVIGPEQGATLPGMTVVCGDSHTSTHGAFAALAH 142

Query: 61  GVGTSDIEQVLATQTLTVPKLKSLRVDIEGTLPAGVYAKDVALGFIREFGTDCAVGHVLE 120
           G+GTS++E VLATQTL   K K++ V ++G LPAGV AKD+ L  I + GT    G+ +E
Sbjct: 143 GIGTSEVEHVLATQTLLARKSKAMLVQVDGALPAGVTAKDIVLAVIGKIGTAGGTGYAIE 202

Query: 121 FAGSVVSGLSMEARMTLCNMATEAGARAGLIAVDDTTINYLQGRPLAPTGEQWTAAVAYW 180
           FAGS +  LSME RMT+CNMA EAGARAG++A DDTT+ YL+GRP +P+G  W  AV+YW
Sbjct: 203 FAGSTIRSLSMEGRMTVCNMAIEAGARAGMVAFDDTTLEYLKGRPFSPSGPHWERAVSYW 262

Query: 181 KTLRSDPGARYDKVLRFNVDTLTPQVTWGTTPAMSVGINESIPSLASFSDPAARKAAENA 240
           ++L SDPGA++D V+  N   + PQVTWGT+P M V ++  +P      DP  R   E A
Sbjct: 263 RSLHSDPGAKFDMVVTLNAAEIKPQVTWGTSPEMVVAVDARVPDPDKEKDPTKRDGMEKA 322

Query: 241 LKYMGLTGGTPIRSLRLDKIFIGSCTNSRIEDLRVAAGIVEGK 283
           L YM L   TPI  +R+DK+FIGSCTNSRIEDLR AA +V GK
Sbjct: 323 LAYMALKPNTPIEDIRIDKVFIGSCTNSRIEDLREAAKVVRGK 365


>gi|300311598|ref|YP_003775690.1| aconitate hydratase
           [Herbaspirillum seropedicae SmR1]
          Length = 873

 Score = 75.1 bits (183), Expect = 7e-15,   Method: Compositional matrix adjust.
 Identities = 64/215 (29%), Positives = 98/215 (45%), Gaps = 20/215 (9%)

Query: 21  PRQGILH---------VVAPELGDTLPGTTIACGDSHTTTHGAFAALAFGVGTSDIEQVL 71
           P  GI+H         V+  + G   P T +   DSHT    A   +A GVG  + E V+
Sbjct: 167 PGNGIMHQINLEKMSPVIHAKDGVAFPDTLVGT-DSHTPHVDALGVIAIGVGGLEAENVM 225

Query: 72  ATQTLTVPKLKSLRVDIEGTLPAGVYAKDVALGFIREFGTDCAVGHVLEFAGSVVSGLSM 131
             +   +     + V++ G    G+ A D+ L           VG  LEF G   + L++
Sbjct: 226 LGRASWMRLPDIIGVELTGRRQPGITATDIVLSLTEFLRKQKVVGAYLEFYGDGAASLTL 285

Query: 132 EARMTLCNMATEAGARAGLIAVDDTTINYLQ--GRPLAPTGEQWTAAVAYWKT--LRSDP 187
             R T+ NMA E GA A + ++D  TI+YL+  GR      EQ      Y K   L SD 
Sbjct: 286 GDRATISNMAPEYGATAAMFSIDQQTIDYLKLTGR----EDEQVKLVETYAKEAGLWSDT 341

Query: 188 GAR--YDKVLRFNVDTLTPQVTWGTTPAMSVGINE 220
            A+  Y++VLRF++ T+   +   + P   + ++E
Sbjct: 342 LAKVEYERVLRFDLSTVVRTLAGPSNPHKRLPVSE 376


>gi|300312283|ref|YP_003776375.1| aconitate hydratase
           [Herbaspirillum seropedicae SmR1]
          Length = 903

 Score = 68.6 bits (166), Expect = 6e-13,   Method: Compositional matrix adjust.
 Identities = 90/326 (27%), Positives = 133/326 (40%), Gaps = 33/326 (10%)

Query: 2   QALEENCSQFDLTYFSLNDPRQGILHVVAPEL---------GDTLPGTTIACGDSHTTTH 52
           Q ++     FD   F +  P  GI+H V  E          G   P T +   DSHTT  
Sbjct: 159 QFMKWGMQAFDT--FGVVPPGFGIVHQVNLEYLARGVHNKTGVYYPDTLVGT-DSHTTMI 215

Query: 53  GAFAALAFGVGTSDIEQVLATQTLTVPKLKSLRVDIEGTLPAGVYAKDVALGFIREFGTD 112
                + +GVG  + E  +  Q +       + V++ G L  GV A D+ L         
Sbjct: 216 NGIGVVGWGVGGIEAEAGMLGQPVYFLTPDVVGVNLTGALREGVTATDLVLTITELLRQT 275

Query: 113 CAVGHVLEFAGSVVSGLSMEARMTLCNMATEAGARAGLIAVDDTTINYLQGRPLAPTGEQ 172
             VG  +EF G     LS+  R T+ NMA E GA  G   VDD TI Y +G     T  +
Sbjct: 276 KVVGKFVEFFGEGTESLSLTDRATIANMAPEYGATMGFFPVDDATIEYFKGT--GRTKAE 333

Query: 173 WTAAVAYWKT--LRSDPGA---RYDKVLRFNVDTLTPQVTWGTTPAMSVGINESIPSLAS 227
             A  AY+K   L   P A    Y + +  ++ T+ P +     P   + I       ++
Sbjct: 334 IDAFEAYFKAQELYGVPKAGQIDYTQEVSLDLGTVAPSLAGPKRPQDRIEIGN---VKST 390

Query: 228 FSDPAARKAAENALKYMGLTGGTPIRS-----LRLDKIFIG---SCTNSRIEDLRVAAGI 279
           FSD   +  AEN         G   ++     L    + I    SCTN+    + +AAG+
Sbjct: 391 FSDLFTKPTAENGFNKKAEDLGASYKNADGVDLHNGDVLIAAITSCTNTSNPSVLLAAGL 450

Query: 280 VEGKKKKXKSGRQYPPGPDRSRLRPG 305
           +   KK  ++G +  P   ++ L PG
Sbjct: 451 L--AKKAVEAGLKVAPHI-KTSLAPG 473


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.318    0.134    0.397 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,099,762
Number of extensions: 48143
Number of successful extensions: 129
Number of sequences better than 1.0e-08: 3
Number of HSP's gapped: 127
Number of HSP's successfully gapped: 3
Length of query: 323
Length of database: 1,619,870
Length adjustment: 85
Effective length of query: 238
Effective length of database: 1,217,395
Effective search space: 289740010
Effective search space used: 289740010
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 131 (55.1 bits)