BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0199.0006.p (442 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312616|ref|YP_003776708.1| major facilitator superfamily p... 143 4e-35 gi|300313879|ref|YP_003777971.1| major facilitator superfamily p... 139 6e-34 gi|300313868|ref|YP_003777960.1| major facilitator superfamily p... 69 6e-13 gi|300309357|ref|YP_003773449.1| major facilitator superfamily (... 69 6e-13 gi|300312423|ref|YP_003776515.1| proline/betaine transporter pro... 69 8e-13 >gi|300312616|ref|YP_003776708.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 414 Score = 143 bits (360), Expect = 4e-35, Method: Compositional matrix adjust. Identities = 124/403 (30%), Positives = 193/403 (47%), Gaps = 48/403 (11%) Query: 15 WNARLTPKHWKTLKGAFLGWIFDGYEALALIVVLSPLLNSLLTTSQAASKPFYAGLVIGI 74 W T +TL F G+ DG++ + + L P L + + S+A AG + Sbjct: 2 WLQETTTSERRTLTATFTGYAVDGFDYM-IYTFLIPTLLAAWSMSKAE-----AGYIATG 55 Query: 75 TLLGWGIGGLVGGVLADYIGRKTVMMWSVFLYALFTGLTAFSDTIVTLCVLRFITGLAMG 134 LL IGG GVLAD GR V+ W+V +A FT L+ F+ + L R + G MG Sbjct: 56 ALLTSAIGGWAAGVLADRYGRVRVLQWTVLWFAFFTFLSGFTTSFGQLFFTRAMQGFGMG 115 Query: 135 SEWSTGIALVAETWPPEAKAKGAGFLQSGF--GWGTLAAAFIWYVLQLIGPIGIDSWRVM 192 EW+ G L+AET + K +G +QS + GW A AF W V ++ P +W+V+ Sbjct: 116 GEWAVGSVLIAETISARHRGKASGLVQSSWAVGWAGSALAF-WGVYAVLPP--ETAWKVL 172 Query: 193 FVVGALPAFFVLYIRKDVDESEEWITALKSQEWGATEQSTEKGGKRPFTLKQVFTEREAV 252 F +G LPA LYIR+ + E ++ AT+ + G+R L+ +F R+ + Sbjct: 173 FWIGVLPALLTLYIRRAISEPAVYL---------ATQARLRESGQRGNFLR-IF-HRDLL 221 Query: 253 RRILLTSLLSVVSIAGWWAVSSWLTEYTRILSAAQGGQFHWDSYVSLLYTT--------G 304 R LL SLL+ +++V++WL + + + ++S+L T+ G Sbjct: 222 RTTLLASLLATGMQGAYYSVTTWLPTFLKT-----------ERHLSVLGTSTYLLVLIGG 270 Query: 305 AIVAYMASGFIADSIGRRWFLFLTFAGSLATTFLIYSYAS---TPMEILIFSPVNGFFTL 361 + Y+ S +++D+IGRR F+ FA L+ SY T +L V GFF Sbjct: 271 SFCGYLTSAWLSDTIGRR-RCFMLFAA--MGIVLVLSYTQMIITDAWMLALGFVLGFFLS 327 Query: 362 GCAFAWLAIYPSELFNTSVRSTAVSFVFNSARLLACLFPIAAG 404 G F+ + Y SELF + VR + F +N R + + P G Sbjct: 328 GI-FSGMGAYLSELFPSDVRGSGQGFCYNFGRAVGAICPALVG 369 >gi|300313879|ref|YP_003777971.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 413 Score = 139 bits (349), Expect = 6e-34, Method: Compositional matrix adjust. Identities = 131/443 (29%), Positives = 210/443 (47%), Gaps = 52/443 (11%) Query: 14 KWNARLTPKHWKTLKGAFLGWIFDGYEALALIVVLSPLLNSLLTTSQAASKPFYAGLVIG 73 W ++T K KT F GW D + + + + P L + + S+A AGL+ G Sbjct: 3 SWYRQVTAKERKTFWACFSGWALDALD-VQMFSLAIPALIAAFSLSKAD-----AGLISG 56 Query: 74 ITLLGWGIGGLVGGVLADYIGRKTVMMWSVFLYALFTGLTAFSDTIVTLCVLRFITGLAM 133 TL+ IGG G L+D GR + ++ ++ FT L+AF+ + L VL+ + G Sbjct: 57 ATLVASAIGGWFAGALSDRYGRVKTLQITIIWFSFFTFLSAFAQSFPQLLVLKALQGFGF 116 Query: 134 GSEWSTGIALVAETWPPEAKAKGAGFLQS--GFGWGTLAAAFIWYVLQLIGPIGIDSWRV 191 G EW+ G L+AET E + K G +QS GWG A ++ VL + P + +WRV Sbjct: 117 GGEWAAGAVLMAETIRAEHRGKAMGTVQSAWAVGWG--GAVLLFSVLFSLLPQEM-AWRV 173 Query: 192 MFVVGALPAFFVLYIRKDVDESEEWITALKSQEWGATEQSTEKGGKRPFTLKQVFTEREA 251 MFV+G LPA V+Y+R+ V E++ ++ Q TL +F + Sbjct: 174 MFVIGLLPALLVVYVRRSVQETDAFLAG----------QKAAASKPAASTLLGIF-RPDV 222 Query: 252 VRRILLTSLLSVVSIAGWWAVSSWLTEYTRILSAAQGGQFHWDSYVSLLYTTG----AIV 307 +R ++ SLL V + G++A+ +WL Y + + ++S+L T G IV Sbjct: 223 LRLTIIGSLLGVGAHGGYYALMTWLPTYLKT-----------EKHLSVLSTGGYLAVIIV 271 Query: 308 AY----MASGFIADSIGRRWFLFLTFAGSLATTFLIYSYAS-TPMEILIFSPVNGFFTLG 362 A+ +AS + D IGRR + FA + T L Y + + ++L+ GFF G Sbjct: 272 AFWCGCIASSQLLDRIGRRRTVAF-FALACVATVLCYIFLPLSDTQMLVCGFPLGFFAAG 330 Query: 363 CAFAWLAIYPSELFNTSVRSTAVSFVFNSARLLACLFPIAAGAMIKAFGGIAPTALAFG- 421 + A++ +EL+ + +R T V F +N R+ + FP+ G M + P A G Sbjct: 331 IPASMGALF-NELYPSGIRGTGVGFCYNFGRVASAGFPVLVGHMSDSM----PLGQAIGI 385 Query: 422 ---SIYVIGLIVPWFLPETVERS 441 Y + ++ LPET R Sbjct: 386 DAAIAYSLVVVAVLMLPETKGRK 408 >gi|300313868|ref|YP_003777960.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 478 Score = 69.3 bits (168), Expect = 6e-13, Method: Compositional matrix adjust. Identities = 53/198 (26%), Positives = 98/198 (49%), Gaps = 9/198 (4%) Query: 23 HWKTLKGAFLGWIFDGYEALALIVVLSPLLNSLLTTSQAASKPFYAGLVIGITLLGWGIG 82 HW+ + + W+ DG E + L+ + +L T A++ + G + + G +G Sbjct: 22 HWRVVIALGVAWVLDGLE-VTLVGSIGSVLERPDTLGLDAAQVGWTG---SLYIAGAVVG 77 Query: 83 GLVGGVLADYIGRKTVMMWSVFLYALFTGLTAFSDTIVTLCVLRFITGLAMGSEWSTGIA 142 LV G L D +GRK + M ++ LY T +AF+ + + R TGL +G E++ + Sbjct: 78 ALVFGRLTDRLGRKKLFMITLALYMAATLASAFATDFLWFALCRLATGLGIGGEYAAINS 137 Query: 143 LVAETWPPEAKAKGAGFLQSGFGWGTLAAAFIWYVL---QLIGPIGIDSWRVMFVVGALP 199 + E P + + + + F G A + V+ +++GP + WRV F++GA+ Sbjct: 138 AIDELVPARVRGRVNLVINASFWIGAALGAGLSLVILDERVLGP--VMGWRVCFLLGAVL 195 Query: 200 AFFVLYIRKDVDESEEWI 217 A ++ +R+ V ES W+ Sbjct: 196 AVAIVLVRRHVPESPRWL 213 >gi|300309357|ref|YP_003773449.1| major facilitator superfamily (MFS) transporter protein [Herbaspirillum seropedicae SmR1] Length = 437 Score = 69.3 bits (168), Expect = 6e-13, Method: Compositional matrix adjust. Identities = 92/372 (24%), Positives = 153/372 (41%), Gaps = 59/372 (15%) Query: 67 YAGLVIGITLLGWG-----IGGLVGGVLADYIGRKTVMMWSVFLYALFTGLTAFSDTIVT 121 Y L++ + G G +GG++ G+ AD GRK M + L L + AF+ T T Sbjct: 46 YTSLLVALATFGIGFFMRPVGGVLLGLYADRKGRKAAMQVIIVLMTLAIAIIAFTPTYAT 105 Query: 122 --------LCVLRFITGLAMGSEWSTGIALVAETWPPEAKAKGAGFLQSGFGWGTLAAAF 173 + V R + G A G E+S+ A + E+ P + + G A Sbjct: 106 IGIAAPIMIVVARMLQGFATGGEYSSSTAFLVESAPAHKRGLYGSWQLVGQCLAVFCGAG 165 Query: 174 IW-YVLQLIGPIGIDS--WRVMFVVGALPAFFVLYIRKDVDESEEWITALKSQEWGATEQ 230 + V + + P +DS WR+ FV+G L L+IR+ + E+++++ + Sbjct: 166 MGALVTRNLSPEALDSWGWRLPFVLGLLIGPVGLWIRRHMHETDDFL------------E 213 Query: 231 STEKGGKRPFTLKQVFTEREAVRRILLTSLLSVVS--IAGWWAVSSWLTEYTRILSAAQG 288 S K P L V R +L S+ V++ +A + + + T + L Sbjct: 214 SANKPNAAPIKLLDVVKTN---LRGVLVSMGQVINGTVAFYVVLVNMPTFANKQLGLPLD 270 Query: 289 GQFHWDSYVSLLYTTGAIVAYMASGFIADSIGRRWFLFLTFAGSLATTFLIYSY-----A 343 F L T A++ + +G ++D +GRR L + SLA ++Y A Sbjct: 271 QAFMVQMIAVALLT--AVIPF--AGALSDRLGRRTVLLYS---SLAFLLMVYPLFVWVSA 323 Query: 344 STPMEILIFSPVNGFFTLGCAFAWLAIYPSELFNTSVRSTAVSFVFNSARLLACLFPIAA 403 + + L+ V +G F + SE F T VRST ++ +N A +L Sbjct: 324 APSLPRLLVMQVLLCIVIGANFGPMPTALSEQFPTRVRSTGLAVAYNLAAML-------- 375 Query: 404 GAMIKAFGGIAP 415 FGG AP Sbjct: 376 ------FGGFAP 381 >gi|300312423|ref|YP_003776515.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 427 Score = 68.9 bits (167), Expect = 8e-13, Method: Compositional matrix adjust. Identities = 82/341 (24%), Positives = 155/341 (45%), Gaps = 51/341 (14%) Query: 73 GITLLGWGIGGLVGGVLADYIGRKTVMMWSVFLYALFTGLTAFSDTIVTLCVL------- 125 G++ +G +V G +D GRK +M S+ L + T + AF + ++ +L Sbjct: 67 GVSFFMRPLGAVVLGAYSDRAGRKAALMLSIMLMTVGTAMIAFMPSYASIGLLAPAGIAL 126 Query: 126 -RFITGLAMGSEWSTGIALVAETWPPEAKAKGAGFLQS----GFGWGTLAAAFIWYVLQ- 179 + I G + G E+ + A + E P GF S G L AA VL Sbjct: 127 GKMIQGFSAGGEFGSSTAFLVEHAPHRR-----GFFSSWQVASQGISLLLAALFGAVLNN 181 Query: 180 LIGPIGIDSW--RVMFVVGALPAFFVLYIRKDVDESEEWITALKSQEWGATEQSTEKGGK 237 ++ P + SW RV F+ G L A +YIR+ +DE+ E+ ++S+EK Sbjct: 182 MLTPEQLASWGWRVPFIFGLLIAPAGIYIRRHLDEAPEF------------KESSEKTNA 229 Query: 238 RPFTLKQVFTEREAVRRILLTSLLSVVSIAGWWAVSSWLTEYTRILSAAQGGQFHWDSYV 297 L+ F ++ R+L+ + +++ VS +L+ Y + Q G W S+ Sbjct: 230 ---PLRDTFAHQK--MRLLIGAGSVIMA-----TVSVYLSLYIPTYAVKQLGLPAWSSFA 279 Query: 298 SLLYTTGAIVAYMAS---GFIADSIGRRWFLFLTFAGSLATTFLIYSY-ASTP--MEILI 351 ++ + ++ ++ S G ++D IGR F+ + A + T+ ++ + ++P +++L+ Sbjct: 280 AM--SVAGLIMFIGSPLVGALSDKIGRTPFMITSSALYIVLTYPMFVFLTNSPGFLQLLL 337 Query: 352 FSPVNGFFTLGCAFAWLAIYPSELFNTSVRSTAVSFVFNSA 392 V G + FA + +++F + R T +S +N A Sbjct: 338 LQTVIGVL-MTMYFAAMPALLADIFPVATRGTGMSLAYNIA 377 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.138 0.440 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,271,594 Number of extensions: 47609 Number of successful extensions: 272 Number of sequences better than 1.0e-08: 20 Number of HSP's gapped: 257 Number of HSP's successfully gapped: 20 Length of query: 442 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 354 Effective length of database: 1,203,190 Effective search space: 425929260 Effective search space used: 425929260 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 132 (55.5 bits)