BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0196.0002.p (163 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313157|ref|YP_003777249.1| long-chain-fatty-acid-CoA ligas... 308 1e-85 gi|300313115|ref|YP_003777207.1| long-chain-fatty-acid-CoA ligas... 113 9e-27 gi|300309365|ref|YP_003773457.1| acetyl-coenzyme A synthetase [H... 102 2e-23 gi|300313986|ref|YP_003778078.1| acyl-CoA synthetase/AMP-acid li... 97 5e-22 gi|300310257|ref|YP_003774349.1| acetyl-coenzyme A synthetase [H... 89 1e-19 >gi|300313157|ref|YP_003777249.1| long-chain-fatty-acid-CoA ligase [Herbaspirillum seropedicae SmR1] Length = 566 Score = 308 bits (790), Expect = 1e-85, Method: Compositional matrix adjust. Identities = 146/165 (88%), Positives = 155/165 (93%), Gaps = 2/165 (1%) Query: 1 VPLGKPGEIAIRGPQVMSGYWNRPDETEKVMTPDGFFKSGDIGIMDERGYTRIVDRKKDM 60 VPLG+PGEIAIRGPQVM GYW RPDET KVMTPDGFFK+GDIG+MDERGYT+IVDRKKDM Sbjct: 402 VPLGQPGEIAIRGPQVMPGYWQRPDETAKVMTPDGFFKTGDIGVMDERGYTKIVDRKKDM 461 Query: 61 ILVSGFNVYPNEIEGVVAAHPGVLECACVGVPDEHTGEAVKLFVVRKDKSLTAEQLMAYC 120 ILVSGFNVYPNE+EGVVA HPGVLECACVGVPDEHTGEAVKLFVVRKDK+L+A QLM YC Sbjct: 462 ILVSGFNVYPNEVEGVVAQHPGVLECACVGVPDEHTGEAVKLFVVRKDKTLSAAQLMDYC 521 Query: 121 KEQFTGYKKPKYIEFRDELPKTNVGKILRRELRDE--KKTEKKAA 163 KEQFTGYKKPKYIEFRDELPKTNVGKILRRELRDE K +K+AA Sbjct: 522 KEQFTGYKKPKYIEFRDELPKTNVGKILRRELRDEPAKAQQKQAA 566 >gi|300313115|ref|YP_003777207.1| long-chain-fatty-acid-CoA ligase [Herbaspirillum seropedicae SmR1] Length = 556 Score = 113 bits (282), Expect = 9e-27, Method: Compositional matrix adjust. Identities = 55/148 (37%), Positives = 86/148 (58%), Gaps = 2/148 (1%) Query: 7 GEIAIRGPQVMSGYWNRPDETEKVMTPDGFFKSGDIGIMDERGYTRIVDRKKDMILVSGF 66 GEI +RGP V GY N P+ K D +F +GD+G +D+ G+ I R DM + G Sbjct: 399 GEICVRGPAVFMGYHNNPEANAKAFKHD-WFHTGDLGHVDQDGFLYITGRSSDMYISGGS 457 Query: 67 NVYPNEIEGVVAAHPGVLECACVGVPDEHTGEA-VKLFVVRKDKSLTAEQLMAYCKEQFT 125 NVYP EIE + HP V E A +GVPD GE+ + + V + + + E L+A+ + + Sbjct: 458 NVYPREIEEALLTHPAVSEVAVLGVPDPKWGESGLAVIVCKAGQQVDGEALLAHLESRIG 517 Query: 126 GYKKPKYIEFRDELPKTNVGKILRRELR 153 YK P+ + D +PK+ GKI++++++ Sbjct: 518 KYKWPRRFIYWDSMPKSGYGKIVKKQIK 545 >gi|300309365|ref|YP_003773457.1| acetyl-coenzyme A synthetase [Herbaspirillum seropedicae SmR1] Length = 549 Score = 102 bits (254), Expect = 2e-23, Method: Composition-based stats. Identities = 61/161 (37%), Positives = 85/161 (52%), Gaps = 9/161 (5%) Query: 1 VPLGKPGEIAIRGPQ--VMSGYWNRPDETEKVMTPDGFFKSGDIGIMDERGYTRIVDRKK 58 +P G G IAIR P + YW + T + D F +GD+G MDE+GY R + R Sbjct: 388 LPHGTVGNIAIRAPDPVMFLRYWRNEEATREKFAGD-FLLTGDLGSMDEQGYIRYLGRND 446 Query: 59 DMILVSGFNVYPNEIEGVVAAHPGVLECACVGVPDEHTGEAVKLFVVRK-----DKSLTA 113 D+I +G+ + P IE + HP V A VGV D E VK FVV K D++L A Sbjct: 447 DVITSAGYRIGPAAIEECLMRHPAVRIAAVVGVKDALRTEVVKAFVVLKDGVTPDEALKA 506 Query: 114 EQLMAYCKEQFTGYKKPKYIEFRDELPKTNVGKILRRELRD 154 E L + + Q ++ P+ I F LP T GKI+R+ L++ Sbjct: 507 E-LQQHVRAQLAAHEYPRLISFVAALPTTATGKIMRKTLKE 546 >gi|300313986|ref|YP_003778078.1| acyl-CoA synthetase/AMP-acid ligase II protein [Herbaspirillum seropedicae SmR1] Length = 546 Score = 97.4 bits (241), Expect = 5e-22, Method: Compositional matrix adjust. Identities = 61/155 (39%), Positives = 85/155 (54%), Gaps = 3/155 (1%) Query: 7 GEIAIRGPQVMSGYWNRPDETEKVMTPDGFFKSGDIGIMDERGYTRIVDRKKDMILVSGF 66 GEI RG M GY T + G+F +GD+G+M+ GY +I DR KD+I+ G Sbjct: 390 GEIMFRGNICMKGYLKNDRATHEAFA-GGWFHTGDLGVMNADGYIKIKDRSKDIIISGGE 448 Query: 67 NVYPNEIEGVVAAHPGVLECACVGVPDEHTGEAVKLFV-VRKDKSLTAEQLMAYCKEQFT 125 N+ E+E V+ HP VL A V PDE GE FV +++ + A +L +C+ Sbjct: 449 NISSVEVEDVLYRHPAVLAAAVVAQPDEKWGETPCAFVELKEGAEVGAAELTEFCRSHLA 508 Query: 126 GYKKPKYIEFRDELPKTNVGKILRRELRDEKKTEK 160 G+K PK I F LPKT+ GKI + ELR K++ Sbjct: 509 GFKVPKAIYF-GPLPKTSTGKIQKFELRKRMKSDS 542 >gi|300310257|ref|YP_003774349.1| acetyl-coenzyme A synthetase [Herbaspirillum seropedicae SmR1] Length = 556 Score = 89.4 bits (220), Expect = 1e-19, Method: Composition-based stats. Identities = 53/157 (33%), Positives = 81/157 (51%), Gaps = 12/157 (7%) Query: 5 KPGEIAI---RGPQV-MSGYWNRPDETEKVMTPDGFFKSGDIGIMDERGYTRIVDRKKDM 60 +PGE+AI R P SGYW + + G++ +GD ++ G + R D+ Sbjct: 393 QPGELAIDIARSPLYWFSGYWKQ----DTPAIAHGYYSTGDNVELEPNGSISFIGRSDDV 448 Query: 61 ILVSGFNVYPNEIEGVVAAHPGVLECACVGVPDEHTGEAVKLFVVRKDKSLTA----EQL 116 I +G+ + P ++E + HP V + A +G+PD E VK FVV D+ + EQL Sbjct: 449 ITSAGYRIGPFDVESALLEHPAVADVAVIGLPDPERTEIVKAFVVLSDQFKASDALREQL 508 Query: 117 MAYCKEQFTGYKKPKYIEFRDELPKTNVGKILRRELR 153 + K + + + P+ IEF D LPKT GK+ R LR Sbjct: 509 AQHVKRRLSAHAYPRAIEFLDALPKTPSGKLQRFVLR 545 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.138 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 520,531 Number of extensions: 20405 Number of successful extensions: 80 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 70 Number of HSP's successfully gapped: 12 Length of query: 163 Length of database: 1,619,870 Length adjustment: 78 Effective length of query: 85 Effective length of database: 1,250,540 Effective search space: 106295900 Effective search space used: 106295900 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 127 (53.5 bits)