BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0195.0009.p (157 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312336|ref|YP_003776428.1| methyl-accepting chemotaxis tra... 167 4e-43 gi|300310594|ref|YP_003774686.1| methyl-accepting chemotaxis pro... 139 2e-34 gi|300310668|ref|YP_003774760.1| methyl-accepting chemotaxis tra... 128 2e-31 gi|300313938|ref|YP_003778030.1| methyl-accepting chemotaxis tra... 93 1e-20 gi|300311974|ref|YP_003776066.1| methyl-accepting chemotaxis I p... 54 5e-09 >gi|300312336|ref|YP_003776428.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 536 Score = 167 bits (422), Expect = 4e-43, Method: Compositional matrix adjust. Identities = 80/146 (54%), Positives = 106/146 (72%) Query: 1 MNVIGNMKIGKRLGLGFAIILALSVLIAVIGVWRLQSVARATQAMMDVPLAKERMIGDWY 60 MN+IGNM IG+RL LGFA+IL S++IA IG+WRL VA+AT+ MM PL ER++ DWY Sbjct: 1 MNIIGNMNIGRRLTLGFALILTFSIIIAGIGLWRLDHVAQATREMMAEPLKTERLMADWY 60 Query: 61 RHTYAGIRRTIAVAKSSDPSLSAFFKPETDEATRISGDLQKKIAELIASDEEKKLYDKIM 120 + AGIRRTIA+AKSSDP+L +F E +++ SG+ QK++ L+++ EEK L+ KI Sbjct: 61 TNLVAGIRRTIAIAKSSDPALGPYFAAEVAASSKSSGEYQKQVEALLSTPEEKALFQKIG 120 Query: 121 ALRKVYLSSRDIIYKAKNEPGADPAM 146 LRK+YLSSRD I KAK + AM Sbjct: 121 ELRKLYLSSRDEINKAKAAGNTEEAM 146 >gi|300310594|ref|YP_003774686.1| methyl-accepting chemotaxis protein I [Herbaspirillum seropedicae SmR1] Length = 541 Score = 139 bits (349), Expect = 2e-34, Method: Compositional matrix adjust. Identities = 72/145 (49%), Positives = 97/145 (66%) Query: 1 MNVIGNMKIGKRLGLGFAIILALSVLIAVIGVWRLQSVARATQAMMDVPLAKERMIGDWY 60 M NMKIG+RL LGFA+ILA + ++ + +WRLQ +A AT MM PL KER++ DWY Sbjct: 1 MRYFANMKIGRRLALGFAVILAAASVVVALSIWRLQGIAAATDEMMHEPLTKERLVSDWY 60 Query: 61 RHTYAGIRRTIAVAKSSDPSLSAFFKPETDEATRISGDLQKKIAELIASDEEKKLYDKIM 120 R + +RRT A+AKSSDPSL+AFF + A+R+S + QK I LI SD+EK ++ K+ Sbjct: 61 RTIHTSVRRTTAIAKSSDPSLAAFFADDAAAASRMSTEQQKAIEALIESDQEKAVFTKLG 120 Query: 121 ALRKVYLSSRDIIYKAKNEPGADPA 145 +RK Y+ RD I KAK E + A Sbjct: 121 EVRKDYIKYRDAISKAKAEGRTEEA 145 >gi|300310668|ref|YP_003774760.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 515 Score = 128 bits (321), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 61/140 (43%), Positives = 89/140 (63%) Query: 3 VIGNMKIGKRLGLGFAIILALSVLIAVIGVWRLQSVARATQAMMDVPLAKERMIGDWYRH 62 +I +++IG RLG GFA++L S+++A +G+WRL++VA TQ MMDVPL ER++ W Sbjct: 1 MIKDIRIGSRLGAGFAVVLVFSMVVAAVGIWRLKTVAVQTQQMMDVPLKTERLVSQWNTL 60 Query: 63 TYAGIRRTIAVAKSSDPSLSAFFKPETDEATRISGDLQKKIAELIASDEEKKLYDKIMAL 122 I+RT V KS DP L AF E +++ S K + +L+AS+EEKK++ I Sbjct: 61 LLIAIQRTTTVVKSHDPELEAFLAKEAAASSKESAQTLKDVEQLLASEEEKKMFAAINVA 120 Query: 123 RKVYLSSRDIIYKAKNEPGA 142 RK +L+ RD IY AK + A Sbjct: 121 RKQFLTIRDQIYAAKKQGDA 140 >gi|300313938|ref|YP_003778030.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 533 Score = 92.8 bits (229), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 52/134 (38%), Positives = 76/134 (56%), Gaps = 2/134 (1%) Query: 6 NMKIGKRLGLGFAIILALSVLIAVIGVWRLQSVARATQAMMDVPLAKERMIGDWYRHTYA 65 N+KIG RL +GF +L L ++ +GVWRL S+ AT M + L KER W+ Sbjct: 3 NLKIGVRLAIGFIALLMLMAVMVALGVWRLGSIGDATDDMTRIALKKERDAVQWHAAIKE 62 Query: 66 GIRRTIAVAKSSDPSLSAFFKPETD-EATRISGDLQKKIAELIASDEEKKLYDKIMALRK 124 RT AV KS D + A+F+ + D ++ RIS LQK++ I EEK+L+D++ LR Sbjct: 63 NGVRTFAVMKSDDDAFQAYFQKQIDAQSARIS-QLQKEVEAAITDAEEKRLFDEVGKLRA 121 Query: 125 VYLSSRDIIYKAKN 138 Y ++R I+ K Sbjct: 122 AYRATRQSIFDVKT 135 >gi|300311974|ref|YP_003776066.1| methyl-accepting chemotaxis I protein [Herbaspirillum seropedicae SmR1] Length = 517 Score = 54.3 bits (129), Expect = 5e-09, Method: Compositional matrix adjust. Identities = 35/145 (24%), Positives = 75/145 (51%), Gaps = 4/145 (2%) Query: 1 MNVIGNMKIGKRLGLGFAIILALSVLIAVIGVWRLQSVARATQAMMDVPLAKERMIGDWY 60 M+++ + +G+RL LGF +I+ L + + + + +L+ V +A Q + + + +W Sbjct: 1 MSLLNRLTVGRRLALGFVVIIGLMLAMLLAMLAQLKQVEQANQILQKEQAERLSLAKEWR 60 Query: 61 RHTYAGIRRTIAVAKSSDPSLSAFFKPETDEATRISGDLQKKIAELIASDEEKKLYDKIM 120 + + R +A+ +++ +L FF + + +QK+ AEL ++E K + +++ Sbjct: 61 ENIVVNVARALALGVTANETLLKFFADDMAGLVTRTTVIQKRYAELDDTEEGKTILERMA 120 Query: 121 ALRKVYLSSRDIIYKAKNEPGADPA 145 RK YL ++ + E GAD A Sbjct: 121 KERKRYL----VVRQQMEEVGADAA 141 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.134 0.378 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 396,745 Number of extensions: 12414 Number of successful extensions: 68 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 69 Number of HSP's successfully gapped: 5 Length of query: 157 Length of database: 1,619,870 Length adjustment: 78 Effective length of query: 79 Effective length of database: 1,250,540 Effective search space: 98792660 Effective search space used: 98792660 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 126 (53.1 bits)