BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0195.0008.p (388 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312606|ref|YP_003776698.1| EAL domain transmembrane protei... 602 e-173 gi|300310189|ref|YP_003774281.1| transmembrane protein [Herbaspi... 328 4e-91 gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodies... 301 7e-83 gi|300314042|ref|YP_003778134.1| signal transduction protein con... 301 8e-83 gi|300311453|ref|YP_003775545.1| signal transduction containing ... 300 1e-82 >gi|300312606|ref|YP_003776698.1| EAL domain transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 695 Score = 602 bits (1551), Expect = e-173, Method: Compositional matrix adjust. Identities = 296/373 (79%), Positives = 336/373 (90%) Query: 1 LAVLTIALLTSLLDARLESRTAKLARSLAEANEELTQLVLHDNLTKLPNRTLLDDRLNQA 60 LA LTIALLTS+LDARLESRTAKLARSLA ANEELTQ+VLHD LT+LPNRTLL+DRLNQA Sbjct: 228 LAALTIALLTSVLDARLESRTAKLARSLAAANEELTQMVLHDQLTRLPNRTLLEDRLNQA 287 Query: 61 INKAAREQGHFALMFCDLDGFKAINDSLGHHVGDLMLVEVAQRVHSIMRSQDTVARLGGD 120 INKAAREQGHFALMF DLDGFKAINDSLGHHVGD +LVEVAQR+ S R+ DTVARLGGD Sbjct: 288 INKAAREQGHFALMFMDLDGFKAINDSLGHHVGDRLLVEVAQRLTSSSRANDTVARLGGD 347 Query: 121 EFVMLVDLKDPDDAVTVADKLVKIINQPFHIEKHELRVSASIGIAIYPEDGSSRHDLVIN 180 EFV+LVDL P+DA+ VA+KLV+ +NQPF +++HELRVSAS+GIAIYPEDG++RHDLVIN Sbjct: 348 EFVILVDLNTPEDAMGVAEKLVEAVNQPFKVDQHELRVSASVGIAIYPEDGATRHDLVIN 407 Query: 181 ADAAMYHTKRSGRNGYHFFEASMNVNAHNQLQWLQDLRLALERNEFLLHYQPKYNSPNGP 240 ADAAMYHTKRSGRNGYHFFE SMN NA QLQWLQDLR+ALER +F L YQPK++ P GP Sbjct: 408 ADAAMYHTKRSGRNGYHFFEPSMNANAQQQLQWLQDLRVALERGQFRLVYQPKFSLPAGP 467 Query: 241 VLGAEALLRWQHPVHGLVGPDEFIGLAERSGLIVPIGAWVLDEACRQMKTWYDIGHEDWK 300 VLGAEALLRW+HPVHGLV PD+FIGLAERSGLI+PIGAWVLDEACRQM+ W+ +G+ DW Sbjct: 468 VLGAEALLRWEHPVHGLVAPDQFIGLAERSGLILPIGAWVLDEACRQMREWFALGYVDWT 527 Query: 301 IAVNLSALQFAQIDLIDLVKETLARHDLPARCLTLEVTESTAMHDAESSLKTLQQIADLG 360 ++VNLSALQFA L+++V+ TL RH LP CLTLEVTESTAMHD E+SL+TL+QIADLG Sbjct: 528 MSVNLSALQFADPHLLEVVRTTLERHQLPPPCLTLEVTESTAMHDTEASLQTLRQIADLG 587 Query: 361 VDISIDDFGTGYS 373 V+ISIDDFGTGYS Sbjct: 588 VEISIDDFGTGYS 600 >gi|300310189|ref|YP_003774281.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 706 Score = 328 bits (842), Expect = 4e-91, Method: Compositional matrix adjust. Identities = 168/364 (46%), Positives = 229/364 (62%), Gaps = 2/364 (0%) Query: 20 RTAKLARSLAEANEELTQLVLHDNLTKLPNRTLLDDRLNQAINKAAREQGHFALMFCDLD 79 R L R + N++L HD LT LPNR LL DR+ A+ A R FA++F DLD Sbjct: 243 RNQVLKRIVGRTNDKLLHFATHDVLTGLPNRALLADRIQHAVEVARRNGKTFAVLFMDLD 302 Query: 80 GFKAINDSLGHHVGDLMLVEVAQRVHSIMRSQDTVARLGGDEFVMLV-DLKDPDDAVTVA 138 GFKAINDSLGH VGD +LV VAQR+ +R +D VAR+GGDEFV++V +L PD ++ Sbjct: 303 GFKAINDSLGHAVGDGLLVAVAQRIRQCLRGEDMVARIGGDEFVVVVGNLSSPDVVEQLS 362 Query: 139 DKLVKIINQPFHIEKHELRVSASIGIAIYPEDGSSRHDLVINADAAMYHTKRSGRNGYHF 198 + ++ + Q F I+ LRV++SIGIA+YP G S L+ NADAAMY K+SGRN Y F Sbjct: 363 ENILAALRQDFQIDDATLRVTSSIGIAVYPNSGDSVDALMKNADAAMYEAKQSGRNTYRF 422 Query: 199 FEASMNVNAHNQLQWLQDLRLALERNEFLLHYQPKYNSPNGPVLGAEALLRWQHPVHGLV 258 FE +M+ +A LQ Q L+ A++ +F LH+QPKY + + G EALLRW HP G + Sbjct: 423 FEPAMHASAMRHLQVRQALQQAIDEQQFRLHFQPKYRGASKELTGLEALLRWTHPQLGEM 482 Query: 259 GPDEFIGLAERSGLIVPIGAWVLDEACRQMKTWYDIGHEDWKIAVNLSALQFAQIDLIDL 318 P EFI +AERSG I+ IG WVL C Q+ W G + K+A+NLS LQ + DL+ Sbjct: 483 APTEFIPIAERSGQILCIGDWVLQAVCEQIARWDAAGMKPVKVALNLSPLQM-RTDLVAR 541 Query: 319 VKETLARHDLPARCLTLEVTESTAMHDAESSLKTLQQIADLGVDISIDDFGTGYSXKPFV 378 + E + + + L E+TE+ AM D E S + + ++ LG DI+IDDFGTGYS ++ Sbjct: 542 IVELVGAAGIAPQRLMFEITETAAMKDVERSQRVIGELQALGFDIAIDDFGTGYSSLAYL 601 Query: 379 PEHY 382 + + Sbjct: 602 AQFH 605 >gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodiesterase with PAS/PAC sensor domains protein [Herbaspirillum seropedicae SmR1] Length = 701 Score = 301 bits (770), Expect = 7e-83, Method: Compositional matrix adjust. Identities = 159/348 (45%), Positives = 218/348 (62%), Gaps = 5/348 (1%) Query: 34 ELTQLVLHDNLTKLPNRTLLDDRLNQAINKAAREQGHF-ALMFCDLDGFKAINDSLGHHV 92 E+ HD LT LPNR +L + L +A+ + AR++G A+ F DLD FK INDSLGH V Sbjct: 261 EMRHQATHDALTGLPNRNMLHEHLQRAL-RVARQKGALTAVAFVDLDQFKFINDSLGHQV 319 Query: 93 GDLMLVEVAQRVHSIMRSQDTVARLGGDEFVMLVDLKDPDDA--VTVADKLVKIINQPFH 150 GD +L VAQR+ + +R D VAR GGDEFV+++ + +A V +++ + +P+ Sbjct: 320 GDELLKTVAQRLQACLREADMVARQGGDEFVLVLQNQTEGEAGIAEVMQRILAAVARPWQ 379 Query: 151 IEKHELRVSASIGIAIYPEDGSSRHDLVINADAAMYHTKRSGRNGYHFFEASMNVNAHNQ 210 E +V+ASIG++ YP DG L+ +AD+AMY K GRN + FF M+ N+ Sbjct: 380 AGDREFQVTASIGVSRYPADGKDVESLLKHADSAMYRAKEQGRNNFQFFAPWMDSQVSNR 439 Query: 211 LQWLQDLRLALERNEFLLHYQPKYNSPNGPVLGAEALLRWQHPVHGLVGPDEFIGLAERS 270 L+ L +LR AL+++EF L+YQPK + +G ++GAEAL+RW+ P G+V PD FI AE + Sbjct: 440 LEMLINLRRALDQDEFKLYYQPKLSLQDGRLIGAEALIRWESPQQGMVPPDRFIPFAEEA 499 Query: 271 GLIVPIGAWVLDEACRQMKTWYDIGHEDWKIAVNLSALQFAQIDLIDLVKETLARHDLPA 330 GLIVPIG WVL ACRQ + W G +AVNLS Q Q L D V LA+ L A Sbjct: 500 GLIVPIGEWVLRTACRQNQAWQQAGLPPIPVAVNLSPRQLNQ-SLPDFVAGVLAQSGLAA 558 Query: 331 RCLTLEVTESTAMHDAESSLKTLQQIADLGVDISIDDFGTGYSXKPFV 378 L LE+TE+ M DAE S+ TL + LG+ IS+DDFGTGYS ++ Sbjct: 559 SWLELEITENVVMKDAEKSVATLHALKRLGLQISVDDFGTGYSSLSYL 606 >gi|300314042|ref|YP_003778134.1| signal transduction protein containing a membrane domain, an EAL and a GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 948 Score = 301 bits (770), Expect = 8e-83, Method: Compositional matrix adjust. Identities = 158/348 (45%), Positives = 215/348 (61%), Gaps = 1/348 (0%) Query: 27 SLAEANEELTQLVLHDNLTKLPNRTLLDDRLNQAINKAAREQGHFALMFCDLDGFKAIND 86 S+ EL D LT L NR +L L QAI A R++ ++F DLD FK +ND Sbjct: 507 SMKRYEAELEFQASRDTLTGLINRHMLRHHLEQAIGVAERQKQALWVVFVDLDRFKFVND 566 Query: 87 SLGHHVGDLMLVEVAQRVHSIMRSQDTVARLGGDEFVMLVDLKDPDDAVTVA-DKLVKII 145 +LGH GD +L VA R+ + +R DTVARLGGDEF+M++ +D T + +++ + Sbjct: 567 TLGHKAGDTLLRTVAGRLRAAVREADTVARLGGDEFIMILPEQDELTLDTRSLQRIMDAV 626 Query: 146 NQPFHIEKHELRVSASIGIAIYPEDGSSRHDLVINADAAMYHTKRSGRNGYHFFEASMNV 205 P + H L ++ S+G+A YP DG+ L+ +AD AMY K++GRN + F+ +MN Sbjct: 627 AAPVTLGGHTLFMTCSVGVACYPNDGTDGETLIKHADIAMYRGKQTGRNNFQFYTPAMNE 686 Query: 206 NAHNQLQWLQDLRLALERNEFLLHYQPKYNSPNGPVLGAEALLRWQHPVHGLVGPDEFIG 265 A +L+ DLR AL+ EFLLHYQPK + +G V+G+EAL+RWQHP G+V P FIG Sbjct: 687 QALERLRIEGDLRNALDHGEFLLHYQPKVDLASGRVIGSEALIRWQHPQLGMVSPARFIG 746 Query: 266 LAERSGLIVPIGAWVLDEACRQMKTWYDIGHEDWKIAVNLSALQFAQIDLIDLVKETLAR 325 LAE +GLI+PIGAWVL AC Q + W G+ D ++VNLS QF Q DL+ + + L Sbjct: 747 LAEETGLILPIGAWVLRAACTQCRQWQLGGYADISVSVNLSVGQFLQKDLVSSIADVLED 806 Query: 326 HDLPARCLTLEVTESTAMHDAESSLKTLQQIADLGVDISIDDFGTGYS 373 LP L LE+TES M D E ++ L + LGV +SIDDFGTGYS Sbjct: 807 TGLPPESLELELTESLMMTDVEHAIGILAGLKKLGVRLSIDDFGTGYS 854 >gi|300311453|ref|YP_003775545.1| signal transduction containing PAS/GGDEF/EAL domains protein [Herbaspirillum seropedicae SmR1] Length = 683 Score = 300 bits (768), Expect = 1e-82, Method: Compositional matrix adjust. Identities = 158/344 (45%), Positives = 212/344 (61%), Gaps = 4/344 (1%) Query: 38 LVLHDNLTKLPNRTLLDDRLNQAINKAAREQGHFALMFCDLDGFKAINDSLGHHVGDLML 97 L +D LT LPNRTLL ++QA+ +A E H +MF DL+ FK IND+LGH GD++L Sbjct: 243 LAYYDALTGLPNRTLLTRTVDQALEEARHENLHGCVMFIDLNRFKPINDTLGHVAGDMLL 302 Query: 98 VEVAQRVHSIMRSQDTVARLGGDEFVMLVDLKDPDDAVTVADKLVKIINQPFHIEKHELR 157 VEVA R+ +R QD VARLG DEF + + D D VA KL+ + + PF I+ HELR Sbjct: 303 VEVAMRLRRALREQDVVARLGADEFAVALFGIDKDSPAYVAQKLLALFDDPFFIDGHELR 362 Query: 158 VSASIGIAIYPEDGSSRHDLVINADAAMYHTKRSGRN---GYHFFEASMNVNAHNQLQWL 214 V ASIG+++YPE+ + L+ AD AMY K+ G N GY + MN N + L+ Sbjct: 363 VGASIGVSLYPENATDTETLLRLADIAMYRAKQGGENNEGGYACYSEEMNRNTLDLLRIE 422 Query: 215 QDLRLALERNEFLLHYQPKYNSPNGPVLGAEALLRWQHPVHGLVGPDEFIGLAERSGLIV 274 L A E NE LL+YQPK + + GAEAL+RWQHP GL+ P EFI +AE +GLIV Sbjct: 423 TGLLHAFEYNELLLYYQPKVDMQTLAITGAEALVRWQHPERGLLLPGEFIPIAEETGLIV 482 Query: 275 PIGAWVLDEACRQMKTWYDIGHEDWKIAVNLSALQFAQIDLIDLVKETLARHDLPARCLT 334 + WVLD CRQ + W D G +IA+N++A +F + L D V L RH L L Sbjct: 483 QLSDWVLDAVCRQARAWKDAGLPPIRIALNVTAREFTR-SLPDRVGAALERHMLSGEWLE 541 Query: 335 LEVTESTAMHDAESSLKTLQQIADLGVDISIDDFGTGYSXKPFV 378 LE+TES MH + + +++I LG+ +S+DDFGTGYS ++ Sbjct: 542 LEITESMLMHSTDRVISIMEKICGLGITVSLDDFGTGYSSLSYL 585 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.136 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,203,567 Number of extensions: 50097 Number of successful extensions: 275 Number of sequences better than 1.0e-08: 44 Number of HSP's gapped: 206 Number of HSP's successfully gapped: 44 Length of query: 388 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 301 Effective length of database: 1,207,925 Effective search space: 363585425 Effective search space used: 363585425 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)