BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0190.0030.p (244 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314042|ref|YP_003778134.1| signal transduction protein con... 323 1e-89 gi|300311871|ref|YP_003775963.1| EAL domain-containing protein [... 84 1e-17 gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodies... 84 1e-17 gi|300311768|ref|YP_003775860.1| PAS/PAC sensor hybrid histidine... 81 7e-17 gi|300311036|ref|YP_003775128.1| signal transduction protein [He... 74 1e-14 >gi|300314042|ref|YP_003778134.1| signal transduction protein containing a membrane domain, an EAL and a GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 948 Score = 323 bits (827), Expect = 1e-89, Method: Compositional matrix adjust. Identities = 162/242 (66%), Positives = 190/242 (78%), Gaps = 10/242 (4%) Query: 1 LLAAAYLYTLSMRSRRIQRTVDERTAELRLTNVLLKEDIHARQQAEQALQLRQRAIEASA 60 LL AAY+ LSMRS RIQ+ V+ERTAELR TN LL +DI AR+ AE+AL+LR +AIEASA Sbjct: 342 LLGAAYMRALSMRSHRIQQLVEERTAELRTTNALLSDDIKARRLAERALRLRHQAIEASA 401 Query: 61 NAIIIMQATAPDYPIEYVNPAFERITGYSAGEVIGHSIRMLESDSGADAHAHGFEEIWSA 120 NAI+I+ A PDY IEYVNPAF RITGYS+ E +G SIR LE G D+ GFE +W A Sbjct: 402 NAILILSAQEPDYTIEYVNPAFARITGYSSTEAVGKSIRFLE---GRDSDPEGFETVWIA 458 Query: 121 MREQREGNVVLRNHNKSGAEVWNDLYIAPVKSEQGTVTHFVAALYDITSMKRYEAELEFQ 180 MREQRE NVVLRN++K G+E+WND YIAPV+ E G VTHFVAALYDITSMKRYEAELEFQ Sbjct: 459 MREQREHNVVLRNYHKDGSELWNDFYIAPVRDESGEVTHFVAALYDITSMKRYEAELEFQ 518 Query: 181 ANRDVLTGLVNRNMLRKVLEEAMAYAERYQRSLWVVFVDLDRFKFVNXGTPAHRRRFAHR 240 A+RD LTGL+NR+MLR LE+A+ AER +++LWVVFVDLDRFKFVN H+ Sbjct: 519 ASRDTLTGLINRHMLRHHLEQAIGVAERQKQALWVVFVDLDRFKFVND-------TLGHK 571 Query: 241 AG 242 AG Sbjct: 572 AG 573 >gi|300311871|ref|YP_003775963.1| EAL domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 1036 Score = 83.6 bits (205), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 64/192 (33%), Positives = 99/192 (51%), Gaps = 11/192 (5%) Query: 38 DIHARQQAEQALQLRQRAIEASANAIIIMQATAPDYPIEYVNPAFERITGYSAGEVIGHS 97 DI R+Q E L++ A ++ +I T + I VN AF ITGYSA E++G Sbjct: 463 DITDRKQIEAELRISAVAFDSREGMLI----TDANSKILRVNKAFTEITGYSAEEIVGKH 518 Query: 98 IRMLESDSGADAHAHGFEEIWSAMRE--QREGNVVLRNHNKSGAEVWNDLYIAPVKSEQG 155 +L SD A F+E+ ++R + +G + R N W L I+ VK++ Sbjct: 519 PNLLRSDRHGPAF---FQEMRESIRRFGKWQGEIWDRRKNGEVYPEW--LTISAVKNKDQ 573 Query: 156 TVTHFVAALYDITSMKRYEAELEFQANRDVLTGLVNRNMLRKVLEEAMAYAERYQRSLWV 215 VTH+V+ +DI+ K E + A D LT L NR +L L++A+A + R + + Sbjct: 574 QVTHYVSTHHDISDRKLAEERIRELAFFDALTRLPNRTLLLDRLKQAIALSLRTKTCAAL 633 Query: 216 VFVDLDRFKFVN 227 +F+DLD FK +N Sbjct: 634 LFIDLDHFKTLN 645 >gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodiesterase with PAS/PAC sensor domains protein [Herbaspirillum seropedicae SmR1] Length = 701 Score = 83.6 bits (205), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 66/193 (34%), Positives = 94/193 (48%), Gaps = 4/193 (2%) Query: 37 EDIHARQQAEQALQLRQRAIEAS-ANAIIIMQATAPDYPIEYVNPAFERITGYSAGEVIG 95 +D+ R++A QALQ +R + NAI + T PD VNPA RI GY + E + Sbjct: 122 QDVTERKEAAQALQEAERRYRSIFENAIEGVFQTTPDGTYIAVNPALARIYGYHSPEDLI 181 Query: 96 HSIRMLESDSGADAHAHGFEEIWSAMREQRE-GNVVLRNHNKSGAEVWNDLYIAPVKSEQ 154 +R + + +E M EQ N R + + G +W V E Sbjct: 182 VGLRDISHQLYVEPERR--QEFMRLMEEQGSVSNFESRVYRRDGEIIWISENARAVYDEA 239 Query: 155 GTVTHFVAALYDITSMKRYEAELEFQANRDVLTGLVNRNMLRKVLEEAMAYAERYQRSLW 214 G ++ + + IT K +EAE+ QA D LTGL NRNML + L+ A+ A + Sbjct: 240 GKLSCYEGTVEAITERKAHEAEMRHQATHDALTGLPNRNMLHEHLQRALRVARQKGALTA 299 Query: 215 VVFVDLDRFKFVN 227 V FVDLD+FKF+N Sbjct: 300 VAFVDLDQFKFIN 312 >gi|300311768|ref|YP_003775860.1| PAS/PAC sensor hybrid histidine kinase [Herbaspirillum seropedicae SmR1] Length = 552 Score = 81.3 bits (199), Expect = 7e-17, Method: Compositional matrix adjust. Identities = 44/122 (36%), Positives = 71/122 (58%), Gaps = 5/122 (4%) Query: 55 AIEASANAIIIMQATAPDYPIEYVNPAFERITGYSAGEVIGHSIRMLESDSGADAHAHGF 114 A+E + +I+ PD PI +VN AF +TGYS+ EV+G + R L+ G + Sbjct: 48 AVEMTRMPMIVSDPNRPDNPIVFVNNAFINMTGYSSAEVVGKNCRFLQ---GPETDRSVV 104 Query: 115 EEIWSAMREQREGNVVLRNHNKSGAEVWNDLYIAPVKSEQGTVTHFVAALYDITSMKRYE 174 E+ A+ E+RE L N+ K+G+ WN L+I+PV +QG + +F ++ DI+ +R + Sbjct: 105 AEVRRAVAERREIATELLNYRKNGSTFWNALFISPVYDQQGNLKYFFSSQLDIS--RRRD 162 Query: 175 AE 176 AE Sbjct: 163 AE 164 >gi|300311036|ref|YP_003775128.1| signal transduction protein [Herbaspirillum seropedicae SmR1] Length = 750 Score = 73.6 bits (179), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 63/202 (31%), Positives = 96/202 (47%), Gaps = 11/202 (5%) Query: 34 LLKEDIHARQQAEQALQLRQRAIEASANAIIIMQATAPD----YPIE----YVNPAFERI 85 LL +++ A +AE A+Q R R+ E ++ +PD Y +E + NPAF R Sbjct: 161 LLMQNLTALLKAEHAIQQRIRSSERQFRTLV---DNSPDVILRYDMEGRCVFANPAFTRE 217 Query: 86 TGYSAGEVIGHSIRMLESDSGADAHAHGFEEIWSAMREQREGNVVLRNHNKSGAEVWNDL 145 TG A + I + + D E + VVL + G V +++ Sbjct: 218 TGLMADQDINTILDRSANRRWDDESCSFGERVRQVAATGVADVVVLEWRHPQGHLVSHEM 277 Query: 146 YIAPVKSEQGTVTHFVAALYDITSMKRYEAELEFQANRDVLTGLVNRNMLRKVLEEAMAY 205 +I P + +A D+T K E +L QAN D L+GL NR +L +L+ +A Sbjct: 278 HIVPEYDADEQIFGTLAIGRDVTRRKEVEFKLRHQANYDPLSGLPNRRLLGDMLQREVAS 337 Query: 206 AERYQRSLWVVFVDLDRFKFVN 227 A+++ L VVF+DLDRFK VN Sbjct: 338 AKKHAYPLAVVFIDLDRFKDVN 359 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.132 0.375 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 621,663 Number of extensions: 22853 Number of successful extensions: 135 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 136 Number of HSP's successfully gapped: 8 Length of query: 244 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 161 Effective length of database: 1,226,865 Effective search space: 197525265 Effective search space used: 197525265 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 129 (54.3 bits)