BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0188.0001.p (316 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310958|ref|YP_003775050.1| nicotinate phosphoribosyltransf... 236 2e-63 gi|300313859|ref|YP_003777951.1| nicotinic acid phosphoribosyltr... 155 3e-39 gi|300310957|ref|YP_003775049.1| branched-chain amino acid ABC t... 97 2e-21 gi|300311772|ref|YP_003775864.1| branched-chain amino acid ABC t... 78 1e-15 >gi|300310958|ref|YP_003775050.1| nicotinate phosphoribosyltransferase [Herbaspirillum seropedicae SmR1] Length = 402 Score = 236 bits (601), Expect = 2e-63, Method: Compositional matrix adjust. Identities = 110/134 (82%), Positives = 117/134 (87%) Query: 67 FSMSAGPAXHFPGAQVEYRFKCRNDNVDLTPYIDEIKAEIAALCRLRFRESELAYLRSFR 126 F+M HFPGAQVEYRFKCRN+NVDL PY+DEI+AEIAALCRLRF+E EL YLRS R Sbjct: 25 FTMMQVVLHHFPGAQVEYRFKCRNENVDLRPYVDEIRAEIAALCRLRFKEDELNYLRSLR 84 Query: 127 FIKSDFVDFLDLFHLNEKYISVTPSPVGNGEIEIQVKGPWLHTILFEVPVLAIVNEVYFR 186 FIKSDFVDFLDLFHLNEKYI+V P P GNGEIEI VKGPWLHTI+FEVPVLAIVNEVYFR Sbjct: 85 FIKSDFVDFLDLFHLNEKYITVQPDPAGNGEIEISVKGPWLHTIMFEVPVLAIVNEVYFR 144 Query: 187 ATQRDPDYETGRAR 200 ATQR PD E GRAR Sbjct: 145 ATQRSPDLEEGRAR 158 Score = 215 bits (547), Expect = 5e-57, Method: Compositional matrix adjust. Identities = 106/138 (76%), Positives = 117/138 (84%), Gaps = 1/138 (0%) Query: 180 VNEVY-FRATQRDPDYETGRARAXARHDSGDPFEWGERLLKHYDNNRVDSKSKVLVFSDG 238 +++VY A RD D + ARHDSGDPFEWGERL++HY +NRVD SKVLVFSD Sbjct: 264 LSDVYGMDAFLRDYDMYFCKLFDGARHDSGDPFEWGERLIQHYRDNRVDPSSKVLVFSDS 323 Query: 239 LDFDKVVSLYLRFKDRAKVAFGVGTNLTNDLGYTPLQVVMKMVRCNGQPVAKLSDTPAKN 298 LDFDKV +LYLRFKDRAKVAFGVGTNLTNDLGYTPLQVVMKMVRCNGQPVAKLSDTP+KN Sbjct: 324 LDFDKVRALYLRFKDRAKVAFGVGTNLTNDLGYTPLQVVMKMVRCNGQPVAKLSDTPSKN 383 Query: 299 MCDDEAYVKYLRQVFEIQ 316 MCDD AYV YLRQVF+I+ Sbjct: 384 MCDDPAYVNYLRQVFQIK 401 >gi|300313859|ref|YP_003777951.1| nicotinic acid phosphoribosyltransferase [Herbaspirillum seropedicae SmR1] Length = 400 Score = 155 bits (393), Expect = 3e-39, Method: Compositional matrix adjust. Identities = 68/112 (60%), Positives = 83/112 (74%) Query: 204 RHDSGDPFEWGERLLKHYDNNRVDSKSKVLVFSDGLDFDKVVSLYLRFKDRAKVAFGVGT 263 RHDSGDPFEWGE+ L HY R+D+ +K LVFSDGLD + + LY F DRA FG+GT Sbjct: 282 RHDSGDPFEWGEKALAHYRKLRLDANTKRLVFSDGLDLPQALRLYRHFSDRAMTGFGIGT 341 Query: 264 NLTNDLGYTPLQVVMKMVRCNGQPVAKLSDTPAKNMCDDEAYVKYLRQVFEI 315 NL+ND G TPL +VMK+ CNGQ VAKLSD+P K +C DE ++ YLRQ+F I Sbjct: 342 NLSNDTGITPLNIVMKLSSCNGQAVAKLSDSPGKTLCKDETFLAYLRQLFGI 393 Score = 93.2 bits (230), Expect = 3e-20, Method: Compositional matrix adjust. Identities = 51/121 (42%), Positives = 68/121 (56%), Gaps = 4/121 (3%) Query: 67 FSMSAGPAXHFPGAQVEYRFKCRND-NVDLTPYIDEIKAEIAALCRLRFRESELAYLRSF 125 F+M PGAQ EY F CRN L ++++ E+ LC LR R EL YL Sbjct: 16 FTMWQALLHSHPGAQAEYTFVCRNQPAFPLAELKEDVERELDHLCCLRLRPEELDYLAQL 75 Query: 126 RFIKSDFVDFLDLFHLNEKYISVTPSPVGNGEIEIQVKGPWLHTILFEVPVLAIVNEVYF 185 R++KSDF+DFL LF K+I V+ + I +GP +H + FE+ VL IVNE+YF Sbjct: 76 RYMKSDFIDFLALFRFQRKFIEVSTD---GDRLCIVARGPQVHVMGFEIFVLYIVNELYF 132 Query: 186 R 186 R Sbjct: 133 R 133 >gi|300310957|ref|YP_003775049.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 413 Score = 97.1 bits (240), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 46/83 (55%), Positives = 61/83 (73%) Query: 1 LLKERRPDVEFVAEQWPALGKMRAEVVVKALRQARPDAIFNAAFGSDLQQFAEEGSRQGL 60 LLK+ RPDVEFV++QWPALGK++A+ V +A+RQ+RPD IFNA FG DLQ+F + G+RQ L Sbjct: 196 LLKQARPDVEFVSDQWPALGKIKADDVARAIRQSRPDGIFNATFGEDLQKFVQAGNRQQL 255 Query: 61 FAASTVFSMSAGPAXHFPGAQVE 83 F V SM++ A +Q E Sbjct: 256 FEGVRVVSMASAGAPSLDPSQSE 278 >gi|300311772|ref|YP_003775864.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 404 Score = 77.8 bits (190), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 39/72 (54%), Positives = 50/72 (69%) Query: 1 LLKERRPDVEFVAEQWPALGKMRAEVVVKALRQARPDAIFNAAFGSDLQQFAEEGSRQGL 60 L+K+ +PDVEFVAEQ P LGK+ A V +AL A+PDAIFNA F +DL +F EG+ +GL Sbjct: 190 LMKQAQPDVEFVAEQAPPLGKIDAGAVTQALADAKPDAIFNALFAADLGKFVREGNTRGL 249 Query: 61 FAASTVFSMSAG 72 F V S+ G Sbjct: 250 FEQVDVVSLLTG 261 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.138 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 931,670 Number of extensions: 36425 Number of successful extensions: 90 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 88 Number of HSP's successfully gapped: 6 Length of query: 316 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 231 Effective length of database: 1,217,395 Effective search space: 281218245 Effective search space used: 281218245 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)