BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0187.0010.p (506 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311543|ref|YP_003775635.1| major facilitator superfamily p... 851 0.0 gi|300312656|ref|YP_003776748.1| alpha-ketoglutarate permease of... 189 6e-49 gi|300309575|ref|YP_003773667.1| proline/betaine transporter pro... 184 1e-47 gi|300313056|ref|YP_003777148.1| major facilitator superfamily p... 181 1e-46 gi|300312423|ref|YP_003776515.1| proline/betaine transporter pro... 180 3e-46 >gi|300311543|ref|YP_003775635.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 553 Score = 851 bits (2199), Expect = 0.0, Method: Compositional matrix adjust. Identities = 457/552 (82%), Positives = 483/552 (87%), Gaps = 50/552 (9%) Query: 1 MATAS---TAHTITPEERKVIFASSLGTVFEWYDFYLYGSLAAIIAKQFFAGTDPNTAFI 57 M TAS TA ITPEE+KVIFASSLGTVFEWYDFYLYGSLAAIIAKQFFAGTDPNTAFI Sbjct: 1 MTTASVNTTARGITPEEKKVIFASSLGTVFEWYDFYLYGSLAAIIAKQFFAGTDPNTAFI 60 Query: 58 FALLAFAAGFIVRPFGALVFGRLGDLVGRKYTFLVTILIMGASTFIVGILPNYNSIGIAA 117 FALLAFAAGFIVRPFGALVFGRLGDLVGRKYTFLVTILIMGASTFIVG+LPNYNSIGI A Sbjct: 61 FALLAFAAGFIVRPFGALVFGRLGDLVGRKYTFLVTILIMGASTFIVGVLPNYNSIGITA 120 Query: 118 PIILIALRILQGLALGGEYGGAATYVAEHAPEGKRGSFTAWIQTTATLGLFLSLLVILGV 177 PIILI LRILQGLALGGEYGGAATYVAEHAPEG+RGSFTAWIQTTAT+GLFLSLLVILGV Sbjct: 121 PIILIILRILQGLALGGEYGGAATYVAEHAPEGRRGSFTAWIQTTATMGLFLSLLVILGV 180 Query: 178 RTAVGEAAFADWGWRIPFLVSIILLGISVWIRLSMNESPAFLKMKSEGKLSKAPLTEAFG 237 RTAVGEAAFADWGWRIPFLVSI+LL ISVWIR+SMNESPAFLKMK+EGK SKAPLTEAFG Sbjct: 181 RTAVGEAAFADWGWRIPFLVSILLLAISVWIRMSMNESPAFLKMKAEGKTSKAPLTEAFG 240 Query: 238 KWKNLKLVILALVGMTAGQAVVWYAGQFYSLFFLTQTLKVDGPLANILIALALLLATPFF 297 +WKNLKLVILALVG+TAGQAVVWY GQFY+LFFLTQTLKVDGP ANILIA ALLLATPFF Sbjct: 241 QWKNLKLVILALVGLTAGQAVVWYTGQFYALFFLTQTLKVDGPTANILIAAALLLATPFF 300 Query: 298 LIFGSLSDKIGRKPIILGGCLIAAVTYFPIFQGLTHYANPALE----------------- 340 L FGSLSDKIGRKPIILGGCLIAAVTYFPIF GLTH+ANPALE Sbjct: 301 LFFGSLSDKIGRKPIILGGCLIAAVTYFPIFSGLTHFANPALEAALQKSPVIVTADPASC 360 Query: 341 ------------------------------TNVAGAPGSVATIKIGDKVLTSYDASGLSK 370 NVAGAPGSVAT+KIGD+++TSYDA+GLSK Sbjct: 361 QFQFNPTGTKKFTSSCDIVKAKLSAASVNYQNVAGAPGSVATVKIGDRIITSYDAAGLSK 420 Query: 371 EDAAAKDKAFSKELADDIKAAGYPTKADPDQMNKPMVLLLLFILVIYVTMVYGPVAAALV 430 EDAAAKDK SK+LADDIKAAGYP+KADP+QMNKPMVLLLLFILVIYVTMVYGP+AA LV Sbjct: 421 EDAAAKDKQLSKDLADDIKAAGYPSKADPEQMNKPMVLLLLFILVIYVTMVYGPIAACLV 480 Query: 431 EMFPTRIRYTSMSMPYHIGNGWFGGLLPTTAFALVAYKGDIYYGLWYPIVIALATFVIGL 490 EMFPTRIRYTSMSMPYHIGNGWFGGLLPTT+FALVA+KGDIYYGLWYPIVIA+ TFVIG+ Sbjct: 481 EMFPTRIRYTSMSMPYHIGNGWFGGLLPTTSFALVAFKGDIYYGLWYPIVIAMITFVIGM 540 Query: 491 LFVKESKDNNIN 502 LFVKE+ NNI+ Sbjct: 541 LFVKETVRNNIH 552 >gi|300312656|ref|YP_003776748.1| alpha-ketoglutarate permease of the major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 457 Score = 189 bits (479), Expect = 6e-49, Method: Compositional matrix adjust. Identities = 122/349 (34%), Positives = 191/349 (54%), Gaps = 16/349 (4%) Query: 4 ASTAHTITP----EERKVIFA---SSLGTVFEWYDFYLYGSLAAIIAKQFFAGTDPNTAF 56 + T TP E RK IFA +S G + EW+DFY+Y A A FF DP + Sbjct: 2 SDTTQASTPLSAAEYRKRIFAILGASSGNLVEWFDFYVYSFCAIYFAPAFFPKGDPTSQL 61 Query: 57 IFALLAFAAGFIVRPFGALVFGRLGDLVGRKYTFLVTILIMGASTFIVGILPNYNSIGIA 116 + FAAGF++RP G +FGR+ D GRK + L+++L+M + V ++P Y +IG Sbjct: 62 LNTAGVFAAGFLMRPIGGWLFGRIADKHGRKTSMLISVLMMCGGSLAVAVMPTYATIGAW 121 Query: 117 APIILIALRILQGLALGGEYGGAATYVAEHAPEGKRGSFTAWIQTTATLGLFLSLLVILG 176 AP +L+ R+ QGL++GGEYG +ATY++E AP G+RG F ++ T G L++LV+ G Sbjct: 122 APALLLLARLFQGLSVGGEYGTSATYMSEVAPNGRRGFFASFQYVTLIGGQLLAVLVLFG 181 Query: 177 VRTAVGEAAFADWGWRIPFLVSIILLGISVWIRLSMNE-SPAFLKMKSEGKLSKAPLTEA 235 ++ + +A WGWR+PF++ + +++++R S+ E S A + K + K L Sbjct: 182 MQQWLTKAELMAWGWRVPFVLGAVGALVAMYLRSSLAETSSAGARKKKDAGTLKGLLQH- 240 Query: 236 FGKWKNLKLVILALVGMTAGQAVVWYAGQFYSLFFLTQTLKVDGPLANILIALALLLATP 295 K L +VG TAG ++++Y Y +L T +D +AN ++ AL + Sbjct: 241 -------KRAFLNVVGFTAGGSLMFYTFTTYMQKYLVNTAGMDPKVANGVMTGALFVYMI 293 Query: 296 FFLIFGSLSDKIGRKPIILGGCLIAAVTYFPIFQGLTHYANPALETNVA 344 IFG++SDKIGR+ +L V FPI L ++P + +A Sbjct: 294 LQPIFGAISDKIGRRNSMLCFAFFGMVGTFPILHFLKDVSSPGVAMALA 342 >gi|300309575|ref|YP_003773667.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 433 Score = 184 bits (467), Expect = 1e-47, Method: Compositional matrix adjust. Identities = 114/332 (34%), Positives = 182/332 (54%), Gaps = 14/332 (4%) Query: 13 EERKVIFASSLGTVFEWYDFYLYGSLAAIIAKQFFAGTDPNTAFIFALLAFAAGFIVRPF 72 + R+ I A+ +G EW+DF +Y A IIAK FF + T+ + A+ F GF +RP Sbjct: 18 QRRRAIVATVIGNGLEWFDFTVYSFFAVIIAKLFFPTGNDLTSLLLAVATFGVGFFMRPV 77 Query: 73 GALVFGRLGDLVGRKYTFLVTILIMGASTFIVGILPNYNSIGIAAPIILIALRILQGLAL 132 G ++ G D VGRK VTIL+M T ++G+ P Y SIG+ AP+I++ R+LQG + Sbjct: 78 GGILLGIYADRVGRKAALSVTILLMAVGTTMIGLAPTYESIGLFAPLIIVVARLLQGFSA 137 Query: 133 GGEYGGAATYVAEHAPEGKRGSFTAWIQTTATLGLFLSLLVILGVRTAVGEAAFADWGWR 192 GGE G A ++ E+APE +R +++WIQ + + + L V V ++ AA A WGWR Sbjct: 138 GGEMGSATAFLTEYAPERERAYYSSWIQASIGVAVLLGAAVGTFVTASLDAAALASWGWR 197 Query: 193 IPFLVSIILLGISVWIRLSMNESPAFLKMKSEGKLSKAPLTEAFGKWKNLKLVILALVGM 252 +PFL+ II+ + +IR + E+P FL+ K + + +PL E +N A M Sbjct: 198 LPFLLGIIIGPVGYYIRHHLEETPTFLEEKDK---TDSPLKEVI---RNFPRETSASFSM 251 Query: 253 TAGQAVVWYAGQFYSLFFLTQTLKV---DGPLANILIALALLLATPFFLIFGSLSDKIGR 309 V Y FY + + LK+ G +A ++ L +++ P I G L+D+IGR Sbjct: 252 VILWTVCTYVLLFYMPTYSVKVLKLPQTTGFIAGMVGGLCIMVLAP---IVGRLADRIGR 308 Query: 310 KPIILGGCLIAAVTYFPIFQGLTHYANPALET 341 +P++ G + + +P+F + + P+L T Sbjct: 309 RPLLSGSAALILMLAYPMFAYIN--SAPSLST 338 >gi|300313056|ref|YP_003777148.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 465 Score = 181 bits (459), Expect = 1e-46, Method: Compositional matrix adjust. Identities = 116/333 (34%), Positives = 184/333 (55%), Gaps = 18/333 (5%) Query: 1 MATASTAHTITPEERKVIFASSLGTVFEWYDFYLYGSLAAIIAKQ-FFAGTDPNTAFIFA 59 +T S AH + +K + +G+ E+YDF++Y + A++I Q FF DP TA I + Sbjct: 2 QSTQSNAHH--QQSKKAAASGWIGSALEYYDFFIYATAASLIFPQLFFPKGDPTTAIIAS 59 Query: 60 LLAFAAGFIVRPFGALVFGRLGDLVGRKYTFLVTILIMGASTFIVGILPNYNSIGIAAPI 119 L + G++ RP GA V G LGD GRK L + +MG ST VG+LP Y+ +G+ AP Sbjct: 60 LATYGVGYVARPIGAFVLGHLGDTHGRKKVLLWCMFLMGFSTIGVGLLPTYDQVGLWAPA 119 Query: 120 ILIALRILQGLALGGEYGGAATYVAEHAPEGKRGSFTAWIQTTATLGLFLSLLVILGVRT 179 +L+ +R+ QG A+ GE GA++ + EH+P G+RG F+++ G L+ V L + Sbjct: 120 LLVIMRLAQGFAVAGEISGASSMILEHSPFGRRGYFSSFTLQGVQAGQILAAAVFLPLAH 179 Query: 180 AVGEAAFADWGWRIPFLVSIILLGISVW-IRLSMNESPAFLKMKSEGKLSKAPLTEAFGK 238 + F WGWRIPFL+S +++ I+ W IR +++E+P F E ++P+ +AF Sbjct: 180 YMPAEQFNVWGWRIPFLLSFLVI-IAGWVIRRTVDETPVFKAADKEELKRRSPVVDAFRD 238 Query: 239 WKN--LKLVILALVGMTAGQAVVW---YAGQ-FYSLFFLTQTLKVDGPLANILIALALLL 292 L++V +AL+ + A ++ YA Q Y + F + NI+ L + Sbjct: 239 CSPNMLRVVCMALMNVIPVVATIFGAAYAVQPAYGIGFAKDVYLWIPVVGNIVAVLVI-- 296 Query: 293 ATPFFLIFGSLSDKIGRKPIILGGCLIAAVTYF 325 P+ G LSD+IGR+P I+ G L+A + F Sbjct: 297 --PY---VGKLSDRIGRRPPIIVGSLLAGLFAF 324 >gi|300312423|ref|YP_003776515.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 427 Score = 180 bits (456), Expect = 3e-46, Method: Compositional matrix adjust. Identities = 107/338 (31%), Positives = 182/338 (53%), Gaps = 21/338 (6%) Query: 3 TASTAHTIT-PEERKVIFASSLGTVFEWYDFYLYGSLAAIIAKQFFAGTDPNTAFIFALL 61 TA+ IT P+ R+ I +SS+G EW+D +YG+ A +IAKQFF D + + + Sbjct: 5 TAAPGADITHPQARRAIISSSIGNALEWFDILIYGAFAVVIAKQFFPTGDDSVSLLLTFA 64 Query: 62 AFAAGFIVRPFGALVFGRLGDLVGRKYTFLVTILIMGASTFIVGILPNYNSIGIAAPIIL 121 F F +RP GA+V G D GRK +++I++M T ++ +P+Y SIG+ AP + Sbjct: 65 TFGVSFFMRPLGAVVLGAYSDRAGRKAALMLSIMLMTVGTAMIAFMPSYASIGLLAPAGI 124 Query: 122 IALRILQGLALGGEYGGAATYVAEHAPEGKRGSFTAWIQTTATLGLFLSLLVILGVRTAV 181 +++QG + GGE+G + ++ EHAP +RG F++W + + L L+ L + + Sbjct: 125 ALGKMIQGFSAGGEFGSSTAFLVEHAPH-RRGFFSSWQVASQGISLLLAALFGAVLNNML 183 Query: 182 GEAAFADWGWRIPFLVSIILLGISVWIRLSMNESPAFLKMKSEGKLSKAPLTEAFGKWKN 241 A WGWR+PF+ +++ ++IR ++E+P F K + + APL + F K Sbjct: 184 TPEQLASWGWRVPFIFGLLIAPAGIYIRRHLDEAPEF---KESSEKTNAPLRDTFAHQK- 239 Query: 242 LKLVILALVGMTAGQAVVWYAGQFYSLFFLTQTLKVDG-----PLANILIA-LALLLATP 295 ++L+I AG ++ + SL+ T +K G A + +A L + + +P Sbjct: 240 MRLLI------GAGSVIMATVSVYLSLYIPTYAVKQLGLPAWSSFAAMSVAGLIMFIGSP 293 Query: 296 FFLIFGSLSDKIGRKPIILGGCLIAAVTYFPIFQGLTH 333 + G+LSDKIGR P ++ + V +P+F LT+ Sbjct: 294 ---LVGALSDKIGRTPFMITSSALYIVLTYPMFVFLTN 328 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.141 0.423 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,338,116 Number of extensions: 47963 Number of successful extensions: 314 Number of sequences better than 1.0e-08: 17 Number of HSP's gapped: 284 Number of HSP's successfully gapped: 18 Length of query: 506 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 417 Effective length of database: 1,198,455 Effective search space: 499755735 Effective search space used: 499755735 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 133 (55.8 bits)