BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0186.0026.p (588 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311064|ref|YP_003775156.1| acetolactate synthase isozyme I... 270 2e-73 gi|300311584|ref|YP_003775676.1| pyruvate dehydrogenase [Herbasp... 126 5e-30 gi|300311743|ref|YP_003775835.1| acetolactate synthase [Herbaspi... 115 7e-27 gi|300310483|ref|YP_003774575.1| benzoylformate decarboxylase [H... 64 4e-11 >gi|300311064|ref|YP_003775156.1| acetolactate synthase isozyme III large subunit [Herbaspirillum seropedicae SmR1] Length = 572 Score = 270 bits (691), Expect = 2e-73, Method: Compositional matrix adjust. Identities = 173/548 (31%), Positives = 280/548 (51%), Gaps = 26/548 (4%) Query: 1 MKAVDAAVYVLEKEGCLQAFGVPGAAINPFYSALKRHGGIQHVLARHVEGASHMAEGYTR 60 + D V L +EG FG PG A+ Y A+ + QH+L RH + A H A+ Y+R Sbjct: 6 LTGADILVRCLAEEGVEHVFGYPGGAVLYIYDAIFKQDKFQHILVRHEQAAVHAADAYSR 65 Query: 61 AKAGNIGLCIGTSGPAGTDMITGLYSAQADSIPILCITGQAPRARLYKEDFQAVDIESIA 120 + + +G+ I TSGP T+ +TGL +A DSIP++ I+GQ P + ++ FQ D I Sbjct: 66 S-SQKVGVAIVTSGPGVTNAVTGLATAYMDSIPMVVISGQVPSTAIGQDAFQECDTVGIT 124 Query: 121 KPVTKWAVTVREPALVPRVFQQAFHLMRSGRPGPVLIDLPFDVQMAEIEFDPDTYEPLPV 180 +P K V++ + ++AF++ +GRPGPVL+D+P D+ M + + Sbjct: 125 RPCVKHNFLVKDVKDLAETVKKAFYIATTGRPGPVLVDIPKDITMHTCAYAYPKAVEMRS 184 Query: 181 YRP--SATRAQIEKALAMLNQSERPLLTIGGGVVNADAAELAVEFAELTGVPVIPTLMAW 238 Y+P QI KAL +L +ERP++ GGG++ A+AA + + G P TLM Sbjct: 185 YKPVDKGHSGQIRKALQLLLSAERPMIYTGGGIILANAAPELNKLVDRLGYPCTNTLMGL 244 Query: 239 GAIPDDHPQMAGMVGLQTSHRYGNATMLASDFVLGVGNRWANRHTGSIEVF-TKDRKFVH 297 GA + GM G+ ++ N M D ++ +G R+ +R G+ + F + RK +H Sbjct: 245 GAYRSSSDKFVGMPGMHGTYE-ANMAMQHCDVLIAIGARFDDRVIGNPKHFASHARKIIH 303 Query: 298 VDIEPTQIGRVFGPDYGIVSDAKAALKLFIEV--AKELKAAGKLPDRSAWLAECQE-RKR 354 +DI+P+ I + D IV + K L+ + A E + G D AW + E RKR Sbjct: 304 IDIDPSSISKRVKVDIPIVGNVKDVLQELLSQLDAAETRQNGAALD--AWWKQINEWRKR 361 Query: 355 TLHRRTHFEEVPIKPQRVYEEMNRAFGRDTCYVSTIGLSQIAAAQFLHVYKDRHWINCGQ 414 + EE IKPQ V +++ D S +G Q+ AAQ+ K R WIN G Sbjct: 362 DCLKFATSEEF-IKPQSVVQKVWEVTKGDAFITSDVGQHQMWAAQYYGFDKPRRWINSGG 420 Query: 415 AGPLGWTISAALGVCAADPQRKVVAISGDYDFQFMIEELAVGAQFNLPYLHVVVNNSYLG 474 G +G + A+GV A+P V I+G+ Q I+ELA Q++L +++NN +LG Sbjct: 421 LGTMGVGLPYAMGVQMANPDATVACITGEASIQMCIQELATCKQYHLTPKIILLNNRFLG 480 Query: 475 LIRQAQRGFEMDYCVQLAFENINA-PELNGYGVDHVAVVEGLGCKAIRVTNPNEI----Q 529 ++RQ Q ++DY + + +++ P+ N + E G +++ P ++ + Sbjct: 481 MVRQWQ---QIDYGSRYSESYMDSLPDFN-------KLAESYGHVGMKIEKPGDVDGALR 530 Query: 530 DAFALAQR 537 +AFA+ R Sbjct: 531 EAFAMKDR 538 >gi|300311584|ref|YP_003775676.1| pyruvate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 564 Score = 126 bits (316), Expect = 5e-30, Method: Compositional matrix adjust. Identities = 137/531 (25%), Positives = 211/531 (39%), Gaps = 32/531 (6%) Query: 5 DAAVYVLEKEGCLQAFGVPGAAINPFYSALKRHGGIQHVLARHVEGASHMAEGYTRAKAG 64 D + L + G Q FGV G A+NP A++R I+ + RH EGA+ A G + G Sbjct: 7 DILIDTLYEIGVRQIFGVVGDALNPLTDAIRRDKRIEWIGVRHEEGAALAAAGQAKL-TG 65 Query: 65 NIGLCIGTSGPAGTDMITGLYSAQADSIPILCITGQAPRARLYKEDFQAVDIESIAKPVT 124 + +C GT+GP ++ GLY A+ D P+L I+G P A + Q E + + V Sbjct: 66 RLAVCCGTTGPGANHLVAGLYEARKDHAPVLAISGGVPAAHRGTDYLQENSPELLFRDVA 125 Query: 125 KWAVTVREPALVPRVFQQAFHLMRSGRPGPVLIDLPFDVQMAEIEFDPDTYEPLPVYRP- 183 ++ + P P VF QA + R G +++P DV A+ + L P Sbjct: 126 AYSQIITSPDQAPAVFHQAIAQAYAQR-GVAHLNIPADVIGAKTSGSVPSLHTLRERTPL 184 Query: 184 SATRAQIEKALAMLNQSERPLLTIGGGVVNADAAELAVEFAELTGVPVIPTLMAWGAIPD 243 S A I+ A ++N + + G G A LA A PV+ T Sbjct: 185 SPPAADIQAAADLINAAGSVAIFAGNGCRGAIDEVLA--LASKLQAPVMHTFRGKDLASY 242 Query: 244 DHPQMAGMVGLQTSHRYGNATMLASDFVLGVGNRWANRHTGSIEVFTKDRKFVHVDIEPT 303 DHP G VGL G + A++ +L +G+ + E K + +D Sbjct: 243 DHPHWIGGVGL-IGGAPGTDALHAAEVMLMLGSDYPYS-----EFLPTKSKVIQIDERGF 296 Query: 304 QIGRVFGPDYGIVSDAKAALKLFIEVAKELKAAGKL-----PDRSAWLAECQERKRTLHR 358 +GR GI A++ +E E K G R W A+ + Sbjct: 297 VLGRRAPVTLGITGSVAPAVQQLLERV-EGKTDGAFLQQANAHRERWNAKLDSAAEI--K 353 Query: 359 RTHFEEVPIKPQRVYEEMNRAFGRDTCYVSTIGLSQIAAAQFLHVYKDRHWINCGQAGPL 418 R H + IKPQ V ++ D +V G+ + ++ + + + Sbjct: 354 RHHKK---IKPQSVARAISDLADDDAAFVVDTGVVTLWCGNWIRQRGGQRILASFNNAAV 410 Query: 419 GWTISAALGVCAADPQRKVVAISGDYDFQFMIEELAVGAQFNLPYLHVVVNNSYLGLIRQ 478 G ++ A G+ A D +R+VV GD F ++ E + LP VV NN GL+ Sbjct: 411 GTSLGQANGIQALDRERQVVVAVGDGGFTMLLGEFMTSVEHRLPVKVVVFNNREWGLVH- 469 Query: 479 AQRGFEMDYCVQLAFENINAPELNGYGVDHVAVVEGLGCKAIRVTNPNEIQ 529 EM+ AFE P D A G + V P E++ Sbjct: 470 ----LEMEEAGMPAFEGSEFPN-----PDFAAFARACGGQGFSVRTPEELE 511 >gi|300311743|ref|YP_003775835.1| acetolactate synthase [Herbaspirillum seropedicae SmR1] Length = 625 Score = 115 bits (289), Expect = 7e-27, Method: Compositional matrix adjust. Identities = 138/538 (25%), Positives = 231/538 (42%), Gaps = 41/538 (7%) Query: 45 ARHVEGASHMAEGYTRAKAGNIGLCIGTS-GPAGTDMITGLYSAQADSIPILCITG---- 99 A + + +H A Y +A + + TS GP T+++T A + +P+L + G Sbjct: 69 AHNEQAMAHSAIAYAKAHLRQRMMAVTTSIGPGATNLLTAAALAHVNRLPVLLLPGDVFV 128 Query: 100 -QAPRARLYK-EDFQ--AVDIESIAKPVTKWAVTVREPALVPRVFQQAFHLMRS-GRPGP 154 + P L + EDF + + +P+++ + P + +A ++ + GP Sbjct: 129 SRRPDPVLQQLEDFNDPGLSVNDAFRPLSRMFDRICYPEQLLTALPRAIQVLTDPAQCGP 188 Query: 155 VLIDLPFDVQMAEIEFDPDTYEP-LPVYRPSATRAQ-IEKALAMLNQSERPLLTIGGGVV 212 V + LP DVQ ++ D + P + V R A AQ +E A+A+L Q+ +PLL GGGV+ Sbjct: 189 VTLALPQDVQTMAYDYPLDFFSPRVIVPRAQAPAAQELEDAVALLRQARQPLLIAGGGVL 248 Query: 213 NADAAELAVEFAELTGVPVIPTLMAWGAIPDDHPQMAGMVGLQTSHRYGNATMLASDFVL 272 +A E FAE GVPV T A+P H G +G+ T NA +D V+ Sbjct: 249 YGNACEHLRRFAEQHGVPVAETQAGKSALPWSHALQMGAIGV-TGSPAANALAQEADVVI 307 Query: 273 GVGNRWANRHTGSIEVFTKDRKFVHVDIEPTQIGRVFGPDYGIVSDAKAALKLFIEVAKE 332 VG R + TGS +F + R +++++ + G + +DA L+ + Sbjct: 308 AVGTRLQDFTTGSHTLFAQAR-LLNLNVNAMDAHKWRG--LALQADAGLGLEALSHALEG 364 Query: 333 LKAAGKLPDRSAWLAE-CQERKRTLHRRTHFEEVPIKPQRVYEEMNRAFGRD------TC 385 ++A + R+ LA+ +ER + +T V + R+ C Sbjct: 365 WRSAPEWHARAHTLAQDWRERVAQITGQTDTGGRLPYDGEVIGAIQRSVADSPSQDIVVC 424 Query: 386 YVSTI--GLSQIAAAQFLHVYKDRHWINCGQAGPLGWTISAALGVCAADPQRKVVAISGD 443 T+ L ++ A Y + +C +G+ ++ LGV A PQR+V+ I GD Sbjct: 425 AAGTLPAELHKLWRAGRPGAYHVEYGYSC-----MGYEVAGGLGVKLAQPQREVIVIVGD 479 Query: 444 YDFQFMIEELAVGAQFNLPYLHVVVNNSYLGLIRQAQRGFEMDYCVQLAFENINAPELN- 502 + M ELA + VV++N G I + Q+ C F N+ A L Sbjct: 480 GSYLMMNSELATSVMLGAKLIVVVLDNRGYGCINRLQQA-----CGGAPFNNMLADCLQA 534 Query: 503 GYG---VDHVAVVEGLGCKAIRVTNPNEIQDAFALAQRWMAEYRVPVVVEIILERVTN 557 G G +D A LG V E++ A A+ A+ V ++ R T+ Sbjct: 535 GPGAPAIDFAAHARSLGALGENVKTITELEAALQRAR--AADRSYLVCIDTDASRTTD 590 >gi|300310483|ref|YP_003774575.1| benzoylformate decarboxylase [Herbaspirillum seropedicae SmR1] Length = 532 Score = 63.5 bits (153), Expect = 4e-11, Method: Compositional matrix adjust. Identities = 64/249 (25%), Positives = 107/249 (42%), Gaps = 8/249 (3%) Query: 6 AAVYVLEKEGCLQAFGVPGAAINPFYSALKRHGGIQHVLARHVEGASHMAEGYTRAKAGN 65 A + ++ + G F PG+ P + ++VL MA+G+ +A +GN Sbjct: 14 AVLDLMRRLGMTSVFANPGSTELPMFRDFPED--FRYVLGLQEAVVVGMADGFAQA-SGN 70 Query: 66 IGLCIGTSGPAGTDMITGLYSAQADSIPILCITGQAPRARLYKEDF-QAVDIESIAKPVT 124 L S + + +++A + P++ GQ R+ L + F A +AKP Sbjct: 71 AALVNLHSAAGVGNAMGNIFTAFRNRTPLIITAGQQARSILPFDPFLGATQATELAKPYL 130 Query: 125 KWAVTVREPALVPRVFQQAFHLMRSGRPGPVLIDLPFDVQMAEIEFDPDTYEPLPVYRPS 184 KW++ A VP +A+H+ GPV + +P D E P T R Sbjct: 131 KWSIEPARAADVPLALARAYHMAMQEPRGPVFVSIPVDDWDQPAELVPPTQV---TSRCR 187 Query: 185 ATRAQIEKALAMLNQSERPLLTIGGGVVNADAAELAVEFAE-LTGVPVIPTLMAWGAIPD 243 A I + A L QS+RP + +G + A A + A+ AE L + + + + P+ Sbjct: 188 PEPAAIARLAAALEQSQRPAIVVGAELDRAGAWDAAIALAETLRARVFVAPMSSRCSFPE 247 Query: 244 DHPQMAGMV 252 DHP AG + Sbjct: 248 DHPLFAGFL 256 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.137 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,935,835 Number of extensions: 85161 Number of successful extensions: 247 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 236 Number of HSP's successfully gapped: 4 Length of query: 588 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 498 Effective length of database: 1,193,720 Effective search space: 594472560 Effective search space used: 594472560 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)