BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0186.0024.p (272 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312370|ref|YP_003776462.1| 3-hydroxyisobutyrate dehydrogen... 129 4e-31 gi|300313519|ref|YP_003777611.1| 6-phosphogluconate dehydrogenas... 100 2e-22 gi|300310292|ref|YP_003774384.1| 3-hydroxyisobutyrate dehydrogen... 96 3e-21 gi|300311332|ref|YP_003775424.1| dehydrogenase [Herbaspirillum s... 70 2e-13 >gi|300312370|ref|YP_003776462.1| 3-hydroxyisobutyrate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 297 Score = 129 bits (323), Expect = 4e-31, Method: Compositional matrix adjust. Identities = 88/250 (35%), Positives = 133/250 (53%), Gaps = 9/250 (3%) Query: 16 LIE-GGATVCTSGAEVAKRADIIIVMVPDTPHVEAVLFSKGGVAEGLSAGKIVVDMSSIS 74 L+E GG T S A VAK AD++I M+P + HVE++ + GV G +++D S+I+ Sbjct: 41 LVEVGGKTEADSMAAVAK-ADVVITMLPASRHVESLYLGEAGVLASAKPGTLLIDCSTIA 99 Query: 75 PIATKGFAQKINAFGCEYLDAPVSGGEVGAKAASLTIMVGGNESAFNDVKPLFELMGKNI 134 P A + A A G E LDAPVSGG GA A +LT MVGG++ AF +P + MGK I Sbjct: 100 PEAARKVAAAAKARGFEMLDAPVSGGTNGATAGTLTFMVGGSKEAFEAAQPYLQKMGKAI 159 Query: 135 TLVGGNGDGQTTKVANQIIVALNIQAVAEALLFASKAGADPARVRQALMGGFASSRILEV 194 G +G GQT KV N +++ + + +EA+ G DP + + + + LEV Sbjct: 160 YHAGESGSGQTVKVCNNMLLGILMIGTSEAIRLGMANGMDPKVLSEIMSKSSGRNWTLEV 219 Query: 195 HG-------ERMVKRTFNPGFRIELHQKDLNLALQGAKALGVSLPNTAATQELMNACAAN 247 + + + GF ++L KDL LA++ A A ++P A + L + + N Sbjct: 220 YNPCPGVMETAPASKGYAGGFGVDLMLKDLGLAVENALATNCAIPLGATARNLYDIHSMN 279 Query: 248 GMSGLDHSAL 257 G LD S++ Sbjct: 280 GAGKLDFSSI 289 >gi|300313519|ref|YP_003777611.1| 6-phosphogluconate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 298 Score = 100 bits (248), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 63/241 (26%), Positives = 114/241 (47%), Gaps = 1/241 (0%) Query: 20 GATVCTSGAEVAKRADIIIVMVPDTPHVEAVLFSKGGVAEGLSAGKIVVDMSSISPIATK 79 G C + AE+A + D++I +V + E VLF G + G +V+ ++++P Sbjct: 45 GGVACANPAELAGKVDVVITLVVNAAQTETVLFGDNGAVSAMKPGSVVISSATVAPDFAI 104 Query: 80 GFAQKINAFGCEYLDAPVSGGEVGAKAASLTIMVGGNESAFNDVKPLFELM-GKNITLVG 138 +++ G LDAPVSGG A + +T+M G + ++ + M GK L Sbjct: 105 ELGKRLVEKGLLLLDAPVSGGAARAASGEMTMMTSGPAEVYAKIEDVLAGMAGKVYRLGD 164 Query: 139 GNGDGQTTKVANQIIVALNIQAVAEALLFASKAGADPARVRQALMGGFASSRILEVHGER 198 +G G K+ NQ++ ++I A AEA+ + G +P + + + +S + E Sbjct: 165 THGIGSKVKIINQLLAGVHIAASAEAMALGLREGVNPDALYEVITHSAGNSWMFENRVPH 224 Query: 199 MVKRTFNPGFRIELHQKDLNLALQGAKALGVSLPNTAATQELMNACAANGMSGLDHSALC 258 ++ + P +++ KDL L L A+ LP +AA ++ + G G D SA+ Sbjct: 225 ILSGDYTPLSAVDIFVKDLGLVLDTARRSKFPLPLSAAAHQMFMMASTAGHGGEDDSAVI 284 Query: 259 R 259 + Sbjct: 285 K 285 >gi|300310292|ref|YP_003774384.1| 3-hydroxyisobutyrate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 288 Score = 95.9 bits (237), Expect = 3e-21, Method: Compositional matrix adjust. Identities = 63/208 (30%), Positives = 115/208 (55%), Gaps = 1/208 (0%) Query: 15 ALIEGGATVCTSGAEVAKRADIIIVMVPDTPHVEAVLFSKGGVAEGLSAGKIVVDMSSIS 74 AL + G S A +A +++++ +P + VE V+ +GG+ + + G ++VD ++ Sbjct: 39 ALAQQGLHCVDSIAALAPAVEMVVLSLPTSDIVEQVVEGEGGLLQHGAPGLVIVDTTTAD 98 Query: 75 PIATKGFAQKINAFGCEYLDAPVSGGEVGAKAASLTIMVGGNESAFNDVKPLFELMGKNI 134 P +T+ A ++ A G E +DAPVSGG GA A++ +++GG+ ++P+ +M Sbjct: 99 PNSTRRLAARLQAAGMELVDAPVSGGPKGAATATMGMVIGGSAETVARIEPVLAVMSAKR 158 Query: 135 TLVGGNGDGQTTKVANQIIVALNIQAVAEALLFASKAGADPARVRQALMGGFASSRILEV 194 VG G G K+ N ++ ++ EA A+ AG D ++ Q L G A+S+++EV Sbjct: 159 VHVGPVGAGHVAKLLNNLVTGTHLLIAGEAARIAAAAGLDARQIFQGLAAGSANSKVIEV 218 Query: 195 -HGERMVKRTFNPGFRIELHQKDLNLAL 221 + + F+ GF ++L +KDL LA+ Sbjct: 219 FYPTWIFNEAFDSGFTMKLMRKDLRLAM 246 >gi|300311332|ref|YP_003775424.1| dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 292 Score = 69.7 bits (169), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 62/200 (31%), Positives = 98/200 (49%), Gaps = 4/200 (2%) Query: 64 GKIVVDMSSISPIATKGFAQKINAFGCEYLDAPVSGGEVGAKAASLTIMVGGNESAFNDV 123 G I V+M++IS A A G Y+ APV G A+A L I+ G E A V Sbjct: 86 GAIHVNMATISVALALRLAGLHQARGVAYVAAPVLGRVNVAEAGQLNILAAGEEQALAAV 145 Query: 124 KPLFELMGKNITLVGGNGD-GQTTKVANQIIVALNIQAVAEALLFASKAGADPA-RVRQA 181 +PL +++G+ +G + K+A ++A I A++EA+ G D A + A Sbjct: 146 QPLLDVLGQKTWRLGRQPEQANAAKLAMNFMIASAIGAMSEAVALVQGYGLDKAGFIELA 205 Query: 182 LMGGFASSRILEVHGERMVKRTFNP-GFRIELHQKDLNLALQGAKALGVSLPNTAATQEL 240 FA+ + + +G+ + F P GF++ L KD+ LAL+ + V L +A ++L Sbjct: 206 TSTAFAAP-VYKGYGQAIADDRFEPAGFKLALGLKDVRLALEAGEQAHVPLSLASALRDL 264 Query: 241 MNACAANGMSGLDHSALCRA 260 A+G LD +AL RA Sbjct: 265 HIDGLAHGEGHLDWAALSRA 284 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.132 0.370 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 826,861 Number of extensions: 33513 Number of successful extensions: 161 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 156 Number of HSP's successfully gapped: 4 Length of query: 272 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 188 Effective length of database: 1,222,130 Effective search space: 229760440 Effective search space used: 229760440 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 130 (54.7 bits)