BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0186.0021.p (147 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310006|ref|YP_003774098.1| pyruvate kinase II protein [Her... 68 3e-13 >gi|300310006|ref|YP_003774098.1| pyruvate kinase II protein [Herbaspirillum seropedicae SmR1] Length = 477 Score = 68.2 bits (165), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 39/99 (39%), Positives = 52/99 (52%) Query: 49 ATVTYTSSGHTSLRAARERPMAPILSITPKIATARKLALVWGVHSTISDQIHNESEMVSA 108 A T SG T+L +R PI +ITP +AT RK AL VH+ Q + M+ Sbjct: 376 AIAALTESGSTALWMSRHNIDIPIFAITPNVATRRKAALFRNVHTLELAQCPDTEAMLKG 435 Query: 109 ACQTALREGFAKKGDQIAITAGMPFGQPGSTNLLRLAQV 147 A L +G +KGD I +T G P GQ G TN L++ +V Sbjct: 436 AQDLLLAQGVVQKGDLIVVTWGEPIGQVGGTNALKILRV 474 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.128 0.358 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 424,608 Number of extensions: 13983 Number of successful extensions: 46 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 46 Number of HSP's successfully gapped: 1 Length of query: 147 Length of database: 1,619,870 Length adjustment: 77 Effective length of query: 70 Effective length of database: 1,255,275 Effective search space: 87869250 Effective search space used: 87869250 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 126 (53.1 bits)