BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0186.0009.p (161 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310634|ref|YP_003774726.1| methyl-accepting chemotaxis dom... 91 6e-20 gi|300312458|ref|YP_003776550.1| methyl-accepting chemotaxis sen... 71 3e-14 gi|300313938|ref|YP_003778030.1| methyl-accepting chemotaxis tra... 71 5e-14 gi|300312219|ref|YP_003776311.1| methyl-accepting chemotaxis tra... 71 5e-14 gi|300310695|ref|YP_003774787.1| methyl-accepting chemotaxis sen... 68 4e-13 >gi|300310634|ref|YP_003774726.1| methyl-accepting chemotaxis domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 568 Score = 90.5 bits (223), Expect = 6e-20, Method: Compositional matrix adjust. Identities = 50/113 (44%), Positives = 76/113 (67%) Query: 15 IAQERSAPSRGSLTASPSRVDRIEDGSRFVAEAGATMEEILGSVDQVSTIMRNIADAGQQ 74 +AQ +A ++ T + V ++E GS V EAG+TM E++ SV +V+ I+ I++A Q+ Sbjct: 447 LAQRSAAAAKEIKTLIDNSVSKVEAGSTLVNEAGSTMREVVESVQRVTEIITGISNASQE 506 Query: 75 QSTGIQAIGAAISDTEQMTQQNAALAEQSAAAAEQMRAQAHHLKVAVSAFSLP 127 Q++GI+ I AI+ +Q+TQQNAAL EQ++AA+E M+ QAH L V AF P Sbjct: 507 QASGIEQIQQAINQMDQVTQQNAALVEQASAASESMQMQAHKLTDLVGAFKTP 559 >gi|300312458|ref|YP_003776550.1| methyl-accepting chemotaxis sensory transducer protein [Herbaspirillum seropedicae SmR1] Length = 684 Score = 71.2 bits (173), Expect = 3e-14, Method: Compositional matrix adjust. Identities = 45/93 (48%), Positives = 68/93 (73%) Query: 34 VDRIEDGSRFVAEAGATMEEILGSVDQVSTIMRNIADAGQQQSTGIQAIGAAISDTEQMT 93 V ++E GS+ VA+AG+TMEE++ SV +V+ I+ I+ A Q+QS+GI +G A++ ++ T Sbjct: 501 VQKVETGSQLVAQAGSTMEEVVSSVRRVTDIVGEISAASQEQSSGITEVGNAVNQMDEAT 560 Query: 94 QQNAALAEQSAAAAEQMRAQAHHLKVAVSAFSL 126 QQNAAL EQ+AAAA+ ++ QA HL V+ F L Sbjct: 561 QQNAALVEQAAAAAKSLQEQAAHLAAVVAGFKL 593 >gi|300313938|ref|YP_003778030.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 533 Score = 70.9 bits (172), Expect = 5e-14, Method: Compositional matrix adjust. Identities = 53/114 (46%), Positives = 75/114 (65%), Gaps = 2/114 (1%) Query: 34 VDRIEDGSRFVAEAGATMEEILGSVDQVSTIMRNIADAGQQQSTGIQAIGAAISDTEQMT 93 V++++DGSR VA AG TM+E++ SV +V+ ++R I A Q+QS GI+ + AI+ ++ T Sbjct: 419 VEKVDDGSRLVARAGDTMQEVVQSVARVTAVVREITLASQEQSDGIEQVNLAITHIDETT 478 Query: 94 QQNAALAEQSAAAAEQMRAQAHHLKVAVSAFSL--PANVARIRLQPQTSAQTPL 145 QQNAAL EQ+AAAA M+ QA L AVS F L A V + +Q TS T + Sbjct: 479 QQNAALVEQAAAAARSMQDQAASLTDAVSVFRLEDDAQVLAVAVQRGTSPSTVV 532 >gi|300312219|ref|YP_003776311.1| methyl-accepting chemotaxis transducer transmembrane I protein [Herbaspirillum seropedicae SmR1] Length = 563 Score = 70.9 bits (172), Expect = 5e-14, Method: Compositional matrix adjust. Identities = 51/118 (43%), Positives = 73/118 (61%), Gaps = 11/118 (9%) Query: 34 VDRIEDGSRFVAEAGATMEEILGSVDQVSTIMRNIADAGQQQSTGIQAIGAAISDTEQMT 93 V++++ GSR V +AG+TMEE++ SV +VS I+R+I+ A +QS GI + AI +Q T Sbjct: 445 VNKVQSGSRLVLQAGSTMEEVVTSVRRVSGIVRDISVASGEQSDGIAQVNQAIGMMDQTT 504 Query: 94 QQNAALAEQSAAAAEQMRAQAHHLKVAVSAFSLPANVARIRLQPQTSAQTPLPRPPSP 151 QQNAAL E++AAA++ +R QA L AVS F L + SA+ P P P Sbjct: 505 QQNAALVEEAAAASQSLREQAKGLAQAVSVFRL-----------ERSAEAPQPESTEP 551 >gi|300310695|ref|YP_003774787.1| methyl-accepting chemotaxis sensory transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 585 Score = 67.8 bits (164), Expect = 4e-13, Method: Compositional matrix adjust. Identities = 59/144 (40%), Positives = 87/144 (60%), Gaps = 17/144 (11%) Query: 34 VDRIEDGSRFVAEAGATMEEILGSVDQVSTIMRNIADAGQQQSTGIQAIGAAISDTEQMT 93 V ++E GS V EAG TM EI+ SV +V+ ++ I+ AG++Q +GI+ + A+ +Q+T Sbjct: 424 VAKVESGSLLVNEAGDTMREIVESVQRVTNMINEISAAGEEQRSGIEQVSQAVGQMDQVT 483 Query: 94 QQNAALAEQSAAAAEQMRAQAHHLKVAVSAFSL-------------PANVARIR--LQPQ 138 QQNAAL EQ+AAA++ ++ QA L AVS F L PA+VA R ++P Sbjct: 484 QQNAALVEQAAAASQSLQEQAQELAQAVSVFRLEAGQALEVAPEPAPASVAASRSGVRPA 543 Query: 139 T-SAQTPLPRPPSPALLAAEPLMP 161 T S++ P P PS A +A P+ P Sbjct: 544 TRSSRLPSPVGPS-ASPSASPMHP 566 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.313 0.122 0.337 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 344,910 Number of extensions: 10888 Number of successful extensions: 149 Number of sequences better than 1.0e-08: 61 Number of HSP's gapped: 147 Number of HSP's successfully gapped: 61 Length of query: 161 Length of database: 1,619,870 Length adjustment: 78 Effective length of query: 83 Effective length of database: 1,250,540 Effective search space: 103794820 Effective search space used: 103794820 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 127 (53.5 bits)