BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0186.0007.p (305 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314076|ref|YP_003778168.1| LysR family transcription regul... 138 5e-34 gi|300311642|ref|YP_003775734.1| 1,4-alpha-glucan branching prot... 119 4e-28 gi|300311818|ref|YP_003775910.1| 1,4-alpha-glucan branching prot... 115 6e-27 gi|300310159|ref|YP_003774251.1| LysR family transcription regul... 77 2e-15 gi|300310436|ref|YP_003774528.1| LysR family transcription regul... 74 1e-14 >gi|300314076|ref|YP_003778168.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 294 Score = 138 bits (348), Expect = 5e-34, Method: Compositional matrix adjust. Identities = 77/194 (39%), Positives = 114/194 (58%), Gaps = 4/194 (2%) Query: 1 MEIYQLRTFAIVARLGHITRAAEVLHVTQPAVTAHIKALEQELGIALFDRSHGRITLTKG 60 M++ QLR F VA G++TRAA+ LH+TQPAV+ IK L++ LG+ LF+RS + LT Sbjct: 1 MDLTQLRAFISVAHEGNLTRAADKLHLTQPAVSLQIKGLQESLGLQLFNRSAAGMVLTAE 60 Query: 61 GELLLPEVEKTLAVFNSVLSKAKEMK---GEVSGKAVIGTLGDPDFLRLGSFLNGLHAAL 117 G LLP E+ LA + A + + GE+SG IGT+ DP+F+RLG FL L Sbjct: 61 GNKLLPLAERVLADVQELRRHAADFRSSTGELSGSLAIGTILDPEFIRLGGFLKLLVERH 120 Query: 118 PLLEIKTRSSPSGLMLDEIVRGDLSAGFYLGRVNDRNLATLPLRGVLYRIVAPMSYNDRL 177 P L + SG +L+EI G L GF+LG + ++ L Y+++AP + +R+ Sbjct: 121 PQLSTQLAHHMSGSVLNEIRAGKLDVGFFLG-APGQGFQSMELTSFTYKVIAPAGWKNRV 179 Query: 178 PGYGWREIAAGXWM 191 G GW+E++ W+ Sbjct: 180 SGKGWKELSRLPWI 193 >gi|300311642|ref|YP_003775734.1| 1,4-alpha-glucan branching protein [Herbaspirillum seropedicae SmR1] Length = 819 Score = 119 bits (297), Expect = 4e-28, Method: Compositional matrix adjust. Identities = 52/69 (75%), Positives = 62/69 (89%) Query: 190 WMKQRAQKQSLQAPVSIYEVHAASWMRILEEEGRSLNWNELAERLIPYAKGMGFTHLELL 249 W++QRAQ Q+ QAP+SIYEVHAASW+R+ E R+L+WNELA+RLIPY KGMGFTH+ELL Sbjct: 319 WLQQRAQGQTRQAPMSIYEVHAASWLRLPGEGNRNLDWNELADRLIPYVKGMGFTHVELL 378 Query: 250 PVMEHPFGG 258 PVMEHPFGG Sbjct: 379 PVMEHPFGG 387 >gi|300311818|ref|YP_003775910.1| 1,4-alpha-glucan branching protein [Herbaspirillum seropedicae SmR1] Length = 804 Score = 115 bits (287), Expect = 6e-27, Method: Compositional matrix adjust. Identities = 49/69 (71%), Positives = 61/69 (88%) Query: 190 WMKQRAQKQSLQAPVSIYEVHAASWMRILEEEGRSLNWNELAERLIPYAKGMGFTHLELL 249 WM +RAQ+QSL AP+SIYEVH ASW R+ E++ R+LNW+ELA++LIPY GMGFTH+EL+ Sbjct: 293 WMAERAQRQSLSAPMSIYEVHPASWRRVPEQDHRNLNWDELADQLIPYVVGMGFTHIELM 352 Query: 250 PVMEHPFGG 258 PVMEHPFGG Sbjct: 353 PVMEHPFGG 361 >gi|300310159|ref|YP_003774251.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 309 Score = 76.6 bits (187), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 57/161 (35%), Positives = 76/161 (47%), Gaps = 7/161 (4%) Query: 1 MEIYQLRTFAIVARLGHITRAAEVLHVTQPAVTAHIKALEQELGIALFDRSHGRITLTKG 60 ME LR F V G + AA LH+ QPA++ IK LE+ELG ALFDRS TLT Sbjct: 1 MEFRHLRYFLGVVEHGSVAEAARRLHIAQPALSRQIKYLEEELGAALFDRSVRGATLTAA 60 Query: 61 GELLLPEVEKTLAVFNSVLSKAKEMKGEVSGKAVIGTLGDPDF---LRLGSFLNGLHAAL 117 G+ L+ + + LA + + + G +G P+F R+ L H A Sbjct: 61 GQQLVIDARRMLADLQETRQRVARVAEGLEGNLRMGI--TPNFGWHPRILGSLRHFHQAE 118 Query: 118 PLLEIKTRSSPSGLMLDEIVRGDLSAGFYLGRV--NDRNLA 156 P + + S L+ I GDL AGF R ND+ LA Sbjct: 119 PQIAVSLEPGLSARQLERIRSGDLDAGFLAWRYPPNDQFLA 159 >gi|300310436|ref|YP_003774528.1| LysR family transcription regulator [Herbaspirillum seropedicae SmR1] Length = 300 Score = 74.3 bits (181), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 53/164 (32%), Positives = 85/164 (51%) Query: 1 MEIYQLRTFAIVARLGHITRAAEVLHVTQPAVTAHIKALEQELGIALFDRSHGRITLTKG 60 +E+ QLR FA VA H RAA LH+TQP ++ I ALE+ LG LF R+ + LT Sbjct: 7 LELRQLRYFAAVAEEMHFGRAAARLHMTQPPLSQTIAALEELLGTPLFQRTKRSVALTAA 66 Query: 61 GELLLPEVEKTLAVFNSVLSKAKEMKGEVSGKAVIGTLGDPDFLRLGSFLNGLHAALPLL 120 G LLPE ++ L ++ + SG + + D+ L L A P + Sbjct: 67 GAALLPEAQRLLQQAAALPDLVRRAASGASGLLSLSFISSADYSILPPLLQRFRAHYPQV 126 Query: 121 EIKTRSSPSGLMLDEIVRGDLSAGFYLGRVNDRNLATLPLRGVL 164 +I+ R + + + L+++++G + AG L + ++ A L +R VL Sbjct: 127 QIELREATTDVQLEDLLQGKIDAGLLLPPLPEKAAALLEVRPVL 170 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.137 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,035,007 Number of extensions: 45737 Number of successful extensions: 197 Number of sequences better than 1.0e-08: 34 Number of HSP's gapped: 190 Number of HSP's successfully gapped: 34 Length of query: 305 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 220 Effective length of database: 1,217,395 Effective search space: 267826900 Effective search space used: 267826900 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)