BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0186.0001.p (58 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310163|ref|YP_003774255.1| response regulator protein [Her... 82 8e-18 gi|300310718|ref|YP_003774810.1| phosphate regulon two-component... 52 8e-09 >gi|300310163|ref|YP_003774255.1| response regulator protein [Herbaspirillum seropedicae SmR1] Length = 239 Score = 82.0 bits (201), Expect = 8e-18, Method: Compositional matrix adjust. Identities = 38/55 (69%), Positives = 47/55 (85%) Query: 1 VAYVLNSGADDFLQRPFEPLELIARINAMMRRYAPLNDRRSIEYAGFSLDRESKK 55 VA VL++GADDFLQRPF+ +EL+ARINA+MRR AP N+RR IEYAGF L R++ K Sbjct: 87 VARVLDNGADDFLQRPFDAVELVARINAVMRRCAPKNNRRVIEYAGFQLQRDTAK 141 >gi|300310718|ref|YP_003774810.1| phosphate regulon two-component response regulator transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 230 Score = 52.4 bits (124), Expect = 8e-09, Method: Composition-based stats. Identities = 22/51 (43%), Positives = 36/51 (70%) Query: 5 LNSGADDFLQRPFEPLELIARINAMMRRYAPLNDRRSIEYAGFSLDRESKK 55 LN GADD++ +PF P EL+AR+ A++RR AP ++++Y S+D E ++ Sbjct: 98 LNEGADDYVTKPFSPKELVARVQALLRRKAPEIGSQALKYRDISIDVERRQ 148 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.137 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 165,831 Number of extensions: 4766 Number of successful extensions: 52 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 53 Number of HSP's successfully gapped: 2 Length of query: 58 Length of database: 1,619,870 Length adjustment: 31 Effective length of query: 27 Effective length of database: 1,473,085 Effective search space: 39773295 Effective search space used: 39773295 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 123 (52.0 bits)