BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0185.0045.p (338 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310297|ref|YP_003774389.1| alcohol dehydrogenase [Herbaspi... 382 e-107 gi|300313771|ref|YP_003777863.1| Zn-dependent alcohol dehydrogen... 152 5e-38 gi|300312106|ref|YP_003776198.1| zinc-type alcohol dehydrogenase... 136 2e-33 gi|300312025|ref|YP_003776117.1| bifunctional glutathione-depend... 83 3e-17 gi|300311093|ref|YP_003775185.1| NADPH:quinone oxidoreductase [H... 78 8e-16 >gi|300310297|ref|YP_003774389.1| alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 341 Score = 382 bits (980), Expect = e-107, Method: Compositional matrix adjust. Identities = 185/338 (54%), Positives = 240/338 (71%), Gaps = 1/338 (0%) Query: 1 MKAAVVEKFGQPLALRDIPLPSPGPGQILVKTEACGVCHTDLHAAHGDWPVKPALPFVPG 60 MKAAVV +FG+PL++ +P+P+P PGQILVK EA GVCHTDLHAAHGDWPVKP PF+PG Sbjct: 5 MKAAVVREFGKPLSIEQVPVPTPAPGQILVKFEASGVCHTDLHAAHGDWPVKPTPPFIPG 64 Query: 61 HEGIGIVVSMGPGVSEVNIGDRVGVPWLYSACGHCEYCLAAWETVCAEAEYGGYTKNGGF 120 HEG G V ++G GV V GDRVGVPWL++ACG C C WET+CAE + GY+ NG F Sbjct: 65 HEGTGYVAAVGAGVKHVKEGDRVGVPWLHTACGCCSPCRTGWETLCAEQQNTGYSVNGSF 124 Query: 121 AEYILADPKYVAHIPEGLAATAAAPIICAGVTSYKGIKECDVKPGEWLAVSGIGGLGHLA 180 AEY LADPK+V H+P+ L AAP++CAGVT YKG+KE +V+PGEW+ +SGIGGLGH+A Sbjct: 125 AEYGLADPKFVGHLPDNLDFGPAAPVLCAGVTVYKGLKETEVRPGEWVVISGIGGLGHMA 184 Query: 181 VQYGKAMGLRVCAIDVDDEKLAHAQRLGAEVLINVRAGDPVAQLRKLTDGGAHGVLITAP 240 VQY KAMG+ V A D+ ++KLA A++LGA++ ++ R + VA+++++ GGAHG L+TA Sbjct: 185 VQYAKAMGMHVVAADIHEDKLALAKKLGADLTVDGRNHNAVAEVQRII-GGAHGALVTAV 243 Query: 241 SLEAFHQGVAMTRKLGTCVLVGLPPGEFPTPLFDVVANCITIRGSFVGPPPGGPPPPPFA 300 S +A Q R GT LVGLPPG+ P+F+ V IT+RGS VG FA Sbjct: 244 SPKAMEQAFGFLRARGTMALVGLPPGDISLPVFNTVLKRITVRGSIVGTRQDLEESLVFA 303 Query: 301 AAGKVRADIELQPLSAINSVFERLQRGEVPSRVVLDFS 338 A GKV A L IN++F R++ G++ R+VLD Sbjct: 304 AEGKVAAHFTWDKLDNINAIFARMEEGKIDGRIVLDLK 341 >gi|300313771|ref|YP_003777863.1| Zn-dependent alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 349 Score = 152 bits (383), Expect = 5e-38, Method: Compositional matrix adjust. Identities = 114/337 (33%), Positives = 173/337 (51%), Gaps = 17/337 (5%) Query: 11 QPLALRDIPLPSPGPGQILVKTEACGVCHTDLHAAHGDWPVKPALPFVPGHEGIGIVVSM 70 +PL I + G + + CG+CH+DLH +W P VPGHE +G V ++ Sbjct: 13 KPLEHLHITRRATGAHDVQIDIAYCGICHSDLHQIRSEW-AGTQYPCVPGHEIVGRVAAV 71 Query: 71 GPGVSEVNIGDRVGVPWLYSACGHCEYCLAAWETVCAE--AEYGGYTKN------GGFAE 122 G VS+ +GD VGV + +C C C E C Y G T + GG+++ Sbjct: 72 GAHVSKFKVGDVVGVGCIVDSCQSCPDCDEGLENYCDNMVGTYNGKTDDAPGWTMGGYSQ 131 Query: 123 YILADPKYVAHIPEGLAATAA-APIICAGVTSYKGIKECDVKPGEWLAVSGIGGLGHLAV 181 I+ +YV +I A AA AP++CAG+T+Y ++ PG+ + V GIGGLGH+ + Sbjct: 132 QIVVHERYVLNIRHPEADLAAVAPLLCAGITTYSPLRHWGAGPGKKVGVVGIGGLGHMGI 191 Query: 182 QYGKAMGLRVCAIDVDDEKLAHAQRLGAEVLINVRAGDPVAQLRKLTDGGAHGVLITAP- 240 + AMG V A D K A A+ LGA+ ++ R + +A K D + V AP Sbjct: 192 KIAHAMGAHVVAFTTSDSKRADAKALGADEVVVSRNAEEMAAHAKSFDFILNTV--AAPH 249 Query: 241 SLEAFHQGVAMTRKLGTCVLVGLPPGEFPTP-LFDVVANCITIRGSFVGPPPGGPPPPPF 299 L+AF +A+ ++ GT LVG P P+P +F+++ ++ GS +G P F Sbjct: 250 DLDAF---LALLKREGTMTLVGAPATPHPSPNVFNLILKRRSLAGSLIGGIPETQEMLDF 306 Query: 300 AAAGKVRADIELQPLSAINSVFERLQRGEVPSRVVLD 336 A + ADIEL IN+ +ER+ +G+V R V+D Sbjct: 307 CAEHGIVADIELIRADEINTAYERMLKGDVKYRFVID 343 >gi|300312106|ref|YP_003776198.1| zinc-type alcohol dehydrogenase transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 374 Score = 136 bits (343), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 117/352 (33%), Positives = 167/352 (47%), Gaps = 40/352 (11%) Query: 11 QPLALRDIPLPSPGPGQILVKTEACGVCHTDLHAAHGDWPVKPALPFVPGHEGIGIVVSM 70 QPL + ++ L PGPG++LV+ +A G+CH+DL G P +P +P GHE VV + Sbjct: 21 QPLKIEELDLDPPGPGEVLVRMKAAGLCHSDLSVITGVRP-RP-VPMALGHEASAEVVEV 78 Query: 71 GPGVSEVNIGDRVGVPWLYSACGHCEYCLAAWETVCAEAEYGGYTKNGG----------- 119 G GV+++ GD V + ++ S CGHC C+ +C E G T G Sbjct: 79 GSGVNDLRAGDLVVLVFVPS-CGHCLPCMEGRPALC---EPGAATNTVGELLSGQRRLRD 134 Query: 120 -------------FAEYILADPKYVAHIPEGLAATAAAPIICAGVTSY-KGIKECDVKPG 165 FA++ + + I + AA CA +T + +VK G Sbjct: 135 KTQPVHHHLGVSAFADHAVVSRRSCVKIEADIDPVQAALFGCAVLTGVGAAVNTAEVKAG 194 Query: 166 EWLAVSGIGGLGHLAVQYGKAMGLR-VCAIDVDDEKLAHAQRLGAEVLINVRAGDPVAQL 224 AV G+GG+G A+ A G R V A+D+ D KLA A+ LGA +N R D V ++ Sbjct: 195 TTAAVLGLGGVGLCAMLGALASGAREVIAVDLHDSKLALARSLGATATVNARDPDAVEKV 254 Query: 225 RKLTDGGAHGVLITAPSLEAFHQGVAMTRKLGTCVLVGLP-PGE-FPTPLFDVVANCITI 282 R LT GG A S++A MTR+ G V GLP PG+ + +VA T+ Sbjct: 255 RALTRGGVDYAFEMAGSVQAMEAAYRMTRRGGMTVTAGLPAPGQHWALQQVSLVAEERTV 314 Query: 283 RGSFVG---PPPGGPPPPPFAAAGKVRADI---ELQPLSAINSVFERLQRGE 328 +GS++G P P AGK+ D E L+ IN F+RL GE Sbjct: 315 KGSYIGSCVPVRDIPRYIGLFQAGKLPVDRLMGERLALADINRGFDRLHGGE 366 >gi|300312025|ref|YP_003776117.1| bifunctional glutathione-dependent formaldehyde dehydrogenase/alcohol dehydrogenase class III oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 368 Score = 83.2 bits (204), Expect = 3e-17, Method: Compositional matrix adjust. Identities = 103/376 (27%), Positives = 166/376 (44%), Gaps = 51/376 (13%) Query: 2 KAAVVEKFGQPLALRDIPLPSPGPGQILVKTEACGVCHTDLHAAHGDWPVKPALPFVPGH 61 +AAV + G+PL + ++ L P G++LV+ +A G+CHTD + G P + P + GH Sbjct: 4 RAAVAWQAGKPLTIEEVELGGPKAGEVLVEIKATGICHTDYYTLSGADP-EGIFPAILGH 62 Query: 62 EGIGIVVSMGPGVSEVNIGDRVGVPWLYSACGHCEYCLAAWETVC--------------A 107 EG G+VV +GPGV + D V +P C C++CL+ +C A Sbjct: 63 EGAGVVVDVGPGVKSLKKDDHV-IPLYTPECRECKFCLSQKTNLCQAIRATQGRGLMPDA 121 Query: 108 EAEYG-------GYTKNGGFAEYILADPKYVAHIPEGLAATAAAPIICAGVTSYKG--IK 158 + + Y F+ YI+ +A I I C GVT+ G + Sbjct: 122 TSRFSLDGKPLFHYMGTSTFSNYIVVPEIALAKIRSDAPFDKVCYIGC-GVTTGVGAVLF 180 Query: 159 ECDVKPGEWLAVSGIGGLGHLAVQYGKAMGL-RVCAIDVDDEKLAHAQRLGAEVLINVR- 216 V+ G + V G+GG+G +Q K +G ++ +D++ + A A++ G IN + Sbjct: 181 TAKVEAGANVVVFGLGGIGLNVIQAAKMVGANKIIGVDLNPAREAMARKFGMTDFINPKD 240 Query: 217 AGDPVAQLRKLTDGGAH-------GVLITAPSLEAFHQGVAMTRKLGTCVLVGLPPG--E 267 + V + +TDGGA SLE H+G G +++G+ E Sbjct: 241 VPNVVDAIIGMTDGGADYSFECIGNTTTMRQSLECCHKG------WGQSIIIGVAAAGQE 294 Query: 268 FPTPLFDVVANCITIRGSFVGPPPGGPPPPP---FAAAGKVRADIELQ---PLSAINSVF 321 T F +V + +GS G G P + GK+ D + PL IN F Sbjct: 295 ISTRPFQLVTGRVW-KGSAFGGARGRTDVPKIVDWYMEGKLNIDDLITHKLPLERINEGF 353 Query: 322 ERLQRGEVPSRVVLDF 337 + ++ GE R V++F Sbjct: 354 DLMKSGE-SIRSVVEF 368 >gi|300311093|ref|YP_003775185.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 325 Score = 78.2 bits (191), Expect = 8e-16, Method: Compositional matrix adjust. Identities = 80/296 (27%), Positives = 127/296 (42%), Gaps = 33/296 (11%) Query: 1 MKAAVVEKFGQPLALR--DIPLPSPGPGQILVKTEACGVCHTDLHAAHGDWPVKPALPFV 58 M+A +++FG P L+ + P+P G++L+K A GV D+ G++PV P + Sbjct: 1 MRAIEIKEFGGPEVLQACERPVPELKAGEVLIKVHAAGVNRPDVFQRTGNYPVPPGASDL 60 Query: 59 PGHEGIGIVVSMGPGVSEVNIGDRVGVPWLYSACGHCEYCLAAWETVCAEAEYGGYTKNG 118 PG E G +V+ G S +GD VCA + G Sbjct: 61 PGLEVAGEIVAGDLGDSGFKLGD----------------------LVCA------LVQGG 92 Query: 119 GFAEYILADPKYVAHIPEGLAATAAAPIICAGVTSYKGIKECDVKPGE--WLAVSGIGGL 176 G+AE+ A +P+GL+A AA + T + + + GE L G G+ Sbjct: 93 GYAEFCAAPLAQCLPVPKGLSALEAAALPENYFTVWSNVFDRGQLSGEETLLVQGGSSGI 152 Query: 177 GHLAVQYGKAMGLRVCAIDVDDEKLAHAQRLGAEVLINVRAGDPVAQLRKLTDGGAHGVL 236 G A+Q +A+G RV A EK + LGAE IN + D A +++LT G V+ Sbjct: 153 GVTAIQIARALGHRVFATAGSAEKCRACEELGAERAINYKTEDFEAVVKELTGGRGVDVI 212 Query: 237 ITAPSLEAFHQGVAMTRKLGTCVLVG-LPPGEFPTPLFDVVANCITIRGSFVGPPP 291 + + + + G V + L + + V+ +TI GS + P P Sbjct: 213 LDMVGGDYLPREIKALADDGRLVFIAQLGGAKGQLDMGQVMRRRLTITGSTLRPRP 268 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.141 0.446 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,170,529 Number of extensions: 51598 Number of successful extensions: 210 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 189 Number of HSP's successfully gapped: 10 Length of query: 338 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 252 Effective length of database: 1,212,660 Effective search space: 305590320 Effective search space used: 305590320 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)