BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0185.0041.p (396 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311720|ref|YP_003775812.1| poly-beta-hydroxyalkanoate synt... 476 e-135 gi|300312303|ref|YP_003776395.1| poly(3-hydroxyalkanoate) synthe... 286 3e-78 gi|300309607|ref|YP_003773699.1| poly(3-hydroxyalkanoate)synthet... 58 1e-09 >gi|300311720|ref|YP_003775812.1| poly-beta-hydroxyalkanoate synthase [Herbaspirillum seropedicae SmR1] Length = 590 Score = 476 bits (1226), Expect = e-135, Method: Compositional matrix adjust. Identities = 230/382 (60%), Positives = 281/382 (73%), Gaps = 5/382 (1%) Query: 19 FRVGKEVAVTPGKVVFHNKLIELIQYAPASKTVHAVPLLFVPAWIMKYYILDLSPQNSLV 78 F G++VAV+PGKVV N+++ELI+YAPA++TV PLL +PAWIMKYYILDLSP NSL+ Sbjct: 208 FMPGRDVAVSPGKVVLRNRIMELIRYAPATETVCPEPLLLIPAWIMKYYILDLSPANSLI 267 Query: 79 KYLVDLGYTVYMISWKNPVAEDRELSMDDYRRLGVMAAIDTIVAQTGSKQINAVGYCLGG 138 +YLV+ G+TV+ +SWKNP DR+LSMDDY G AA+D + + ++++ VGYCLGG Sbjct: 268 RYLVEKGHTVFCVSWKNPAPADRDLSMDDYLDSGFHAALDAVNSACPDRKVHGVGYCLGG 327 Query: 139 TLLSIAAASMARDGDNRLASMSLFASQVDFEEPGELSLFIDESQVSFLEAVMWDQGYLDT 198 TLLSIAAA+MARDGD RLAS++L A+Q DF EPGEL LFIDESQ+S LEA M G L Sbjct: 328 TLLSIAAAAMARDGDQRLASLTLLAAQTDFTEPGELGLFIDESQISLLEAQMQQAGTLSQ 387 Query: 199 KQMAGAFQLLRSNDLIWSRRLHQYLLGLPEQKX-LMAWNADATRMPYRMHSEYLRQLFLK 257 QMA AFQ+LRS DL+WSR + QYL+G+P LM WNADATRMP MHS+YLR+LFL Sbjct: 388 SQMAAAFQMLRSYDLLWSRVIRQYLMGVPAHNSDLMEWNADATRMPALMHSQYLRRLFLD 447 Query: 258 NDLAEGRYLTDGRPISLTDIRLPVFAVGTVTDHVAPWRSVFKIHRLTDTDVTFVLTSGGH 317 +DL+EGRY DG+P+ L D+ LPVF VGTV DHVAPWRSV+K+H L ++TFVL SGGH Sbjct: 448 DDLSEGRYEVDGKPVLLADVNLPVFLVGTVRDHVAPWRSVYKLHSLCPAEITFVLASGGH 507 Query: 318 NAGVVSPPGHPHRSYQIATHTDKQSMVDASAWQAGVERHEGSWWPAWEAWLRKRSGKRVA 377 NAG+VSPPGHP +QI+T S A W AG GSWWPAW WL +RSG V Sbjct: 508 NAGIVSPPGHPRSQFQISTRAAGASYRSADEWMAGTSVTTGSWWPAWADWLAQRSGTPVP 567 Query: 378 PPPM---PTHGLA-APGKYVMQ 395 M H LA APG Y+M+ Sbjct: 568 AAGMGYDKAHSLADAPGNYIME 589 >gi|300312303|ref|YP_003776395.1| poly(3-hydroxyalkanoate) synthetase [Herbaspirillum seropedicae SmR1] Length = 588 Score = 286 bits (731), Expect = 3e-78, Method: Compositional matrix adjust. Identities = 161/404 (39%), Positives = 229/404 (56%), Gaps = 15/404 (3%) Query: 1 MSDWRKSVPGQHDNENSDFRVGKEVAVTPGKVVFHNKLIELIQYAPASKTVHAVPLLFVP 60 ++D +K Q D + F VG++V + G V+F N+L +LIQY P +KTVH VPLL VP Sbjct: 180 LADLQKGHVSQTDE--TAFEVGRDVGASEGSVIFENELFQLIQYKPLTKTVHEVPLLIVP 237 Query: 61 AWIMKYYILDLSPQNSLVKYLVDLGYTVYMISWKNPVAEDRELSMDDYRRLGVMAAIDTI 120 I K+YILDL P NSLV+Y V+ G+TV++ISW+N A + D Y V+ +I + Sbjct: 238 PCINKFYILDLQPHNSLVRYTVEQGHTVFLISWRNADAAVETATWDQYVDDAVVRSIHIV 297 Query: 121 VAQTGSKQINAVGYCLGGTLLSIAAASMARDGDNRLASMSLFASQVDFEEPGELSLFIDE 180 + QINA+G+C+GGT+LS A A +A G++ +AS++L + +DF + G + ++IDE Sbjct: 298 QEISKQPQINALGFCVGGTILSCALALLAARGEDPVASLTLLTTFLDFSDTGAIDVYIDE 357 Query: 181 SQVSFLEAVMWDQGYLDTKQMAGAFQLLRSNDLIWSRRLHQYLLG-LPEQKXLMAWNADA 239 Q++ E ++ +G + + A AF LR NDL+W+ YL G P L+ WNAD+ Sbjct: 358 MQLALREQMIGQRGLMAGRDFASAFSSLRPNDLLWNYVESNYLKGEKPSAFDLLYWNADS 417 Query: 240 TRMPYRMHSEYLRQLFLKNDLAE-GRYLTDGRPISLTDIRLPVFAVGTVTDHVAPWRSVF 298 T +P M YLR ++L+N L E GR G I L I P F + DH+ PW+S + Sbjct: 418 TNLPGPMFCYYLRHMYLENALKEPGRLTVGGEKIDLRKINAPAFIYASRDDHIVPWKSAY 477 Query: 299 KIHRLTDT----DVTFVLTSGGHNAGVVSPPGHPHRSYQIATHTDKQSMVDASAWQAGVE 354 L +T + FVL + GH AGV++P R+Y I S D AW AG Sbjct: 478 ASLGLINTAKRSNNRFVLGASGHIAGVINPASKNKRNYWINDKAKAGSGAD--AWLAGAT 535 Query: 355 RHEGSWWPAWEAWLRKRSGKRVAPPPMP-----THGLAAPGKYV 393 H GSWW W A+L K G++VA P H APG+YV Sbjct: 536 EHPGSWWGQWAAFLAKHGGRKVAAPKAAGSTRYKHIEPAPGRYV 579 >gi|300309607|ref|YP_003773699.1| poly(3-hydroxyalkanoate)synthetase [Herbaspirillum seropedicae SmR1] Length = 376 Score = 58.2 bits (139), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 69/290 (23%), Positives = 122/290 (42%), Gaps = 30/290 (10%) Query: 44 YAPASKTVHAVPLLFV-PAWIMKYYILDLSPQNSLVKYLVDLGYT-VYMISWKNPVAEDR 101 Y A VP L V P I D SLV+ L+ G V +I W + A+ + Sbjct: 76 YGSADAGHATVPTLIVAPYAGHTSVIADYDQGQSLVETLLGNGVDHVLLIDWHSASADMK 135 Query: 102 ELSMDDYRRLGVMAAIDTIVAQTGSKQINAVGYCLGGTLLSIAAASMARDGDNRLASMSL 161 +L +D+Y +A + ++ + G + + VG C GG + ++ AA ++A + L Sbjct: 136 DLEIDNY-----LADLCVVIDELGGR-VRLVGLCQGGWISAMIAARFP----EKVARLVL 185 Query: 162 FASQVDFEE-PGELSLFIDESQVSFLEAVMWDQGYLDTKQMAGAFQL-----LRSNDLIW 215 S +D G L + + F E ++ G L M G+F L + ++ + Sbjct: 186 AGSPIDAGAGEGPLKQMVQRLPLRFYEDLVRSGGGL----MRGSFMLQGWKNMHPDEHYF 241 Query: 216 SRRLH--QYL---LGLPEQKXLMAWNADATRMPYRMHSEYLRQLFLKNDLAEGRYLTDGR 270 + L Q++ L +++ AW +P R + + + +F N LA G+Y+ G+ Sbjct: 242 NEHLELLQHIDDPAYLAKRETFAAWYETTVDLPGRWYLQAIGHIFKDNLLARGKYVALGQ 301 Query: 271 PISLTDIRLPVFAVGTVTDHVAPWRSVFKIHRLTDT---DVTFVLTSGGH 317 + L I P++ + D + V L T +T + GGH Sbjct: 302 QLDLRQITCPLYLLAGERDDITTPEQVLNAAALVGTPAAQITQKIVPGGH 351 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.134 0.418 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,364,763 Number of extensions: 57201 Number of successful extensions: 163 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 158 Number of HSP's successfully gapped: 3 Length of query: 396 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 309 Effective length of database: 1,207,925 Effective search space: 373248825 Effective search space used: 373248825 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)