BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0185.0040.p (623 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312669|ref|YP_003776761.1| Mg(2+) transport ATPase [Herbas... 721 0.0 gi|300311691|ref|YP_003775783.1| cation transport ATPase [Herbas... 209 4e-55 gi|300310937|ref|YP_003775029.1| heavy metal translocating P-typ... 78 2e-15 gi|300310933|ref|YP_003775025.1| metal-transporting P-type ATPas... 69 8e-13 >gi|300312669|ref|YP_003776761.1| Mg(2+) transport ATPase [Herbaspirillum seropedicae SmR1] Length = 920 Score = 721 bits (1860), Expect = 0.0, Method: Compositional matrix adjust. Identities = 344/624 (55%), Positives = 456/624 (73%), Gaps = 6/624 (0%) Query: 2 TSFDRGIDKYVHLLMRIMLVMVPLVFLLNGITKGDWLEALLFAVAIAVGLAPEMLPMIIT 61 T+F G++K LL+R MLVM P+VF +NG TKGDW+EA LF +++AVGL PEMLPMI+T Sbjct: 301 TAFQAGVNKVSWLLIRFMLVMTPVVFFINGFTKGDWVEAFLFGLSVAVGLTPEMLPMIVT 360 Query: 62 INLAKGALAMSEKKVIVKRLNAIQNFGAMNILCTDKTGTLTQDRIILEKHVDIDGVDSQK 121 LAKGA+A+S KKVIVKRL+AIQNFGAM++LCTDKTGTLTQD+I LE+H DI G + Sbjct: 361 STLAKGAVALSRKKVIVKRLDAIQNFGAMDVLCTDKTGTLTQDKIFLERHTDIHGEPDDE 420 Query: 122 VVEYAYLNSFFQTGLKNLLDMAILSYPGMQEKVEPDRHFHKIDELPFDFERRRMSVIVEQ 181 V++YAYLNS++QTGLKNLLD+A+L + +Q ++ + K+DE+PFDF+RRRMSV+V + Sbjct: 421 VLQYAYLNSYYQTGLKNLLDVAVLEHAELQREMSIASAYRKVDEIPFDFQRRRMSVVVSE 480 Query: 182 ANHTRLLICKGAPEEILSICTDAEHNGKAVPLAPHHGAELSKTVDDLNDDGFRVIAVACK 241 LICKGA EEI+S+C+ A NG+ VP+ P E+ T LN +G RV+ VA K Sbjct: 481 REDHHELICKGAVEEIVSVCSRARVNGEVVPMTPALLEEIRATTGSLNAEGLRVVGVAAK 540 Query: 242 ELPENGATATNEFSNNDESGLTLVGYIAFLDPPKDSAAEAIASLHAYGVTVKVLSGDNGA 301 +LP +S DES L L+GYIAFLDPPK+S A A+A+L A+GV VK+L+GDN Sbjct: 541 DLPPTKEV----YSLADESDLVLIGYIAFLDPPKESTAPALAALRAHGVKVKILTGDNEL 596 Query: 302 VVRNVCRHVGLPGEQAVLGSDIDALSDEAVGELAERIHIFAKLNPQQKARIIRCLQQRHH 361 V +CR VGL + +LGS ++ +SD + E E + +FAKL+P K RI+R L R H Sbjct: 597 VTAKICREVGLEVKGMLLGSYVEKMSDAELSEAVETVTVFAKLSPAHKERIVRVLHDRGH 656 Query: 362 VVGYLGDGINDGAALKTADVGISVDSAVDIAKESADIILMRKSLVVLKDGVLEGRKVFGN 421 VVG++GDGIND AL+ AD+GISVD+AVDIAKE+AD+IL+ KSL+VL++GVLEGRK F N Sbjct: 657 VVGFMGDGINDAPALRAADIGISVDTAVDIAKEAADLILLEKSLMVLEEGVLEGRKTFAN 716 Query: 422 ITKYIKMSSSSAFGNMLSVLGASAILPFLPMAPVQILLNNLLYDFSQTTIATDHVDAEYL 481 + KYIKM++SS FGN+ SVL ASA LPFLPM P+ +L+ NLLYD SQ +I D+VD E+L Sbjct: 717 MLKYIKMTASSNFGNVFSVLIASAFLPFLPMLPLHLLVQNLLYDVSQISIPFDNVDKEFL 776 Query: 482 KEPRRWEILDIGRFMMVLGPVSSLFDYLTFGVLWYLFGAGN--QSLLFQSGWFVESLLSQ 539 ++P+RW+ DIGRFM+ GP+SS+FD TF ++W++FGA + LFQSGWF+E LLSQ Sbjct: 777 EKPQRWDAGDIGRFMVFFGPISSIFDITTFALMWFIFGASSPEHQTLFQSGWFIEGLLSQ 836 Query: 540 TLVVHVIRTGKIPFIQSKPSLPLLATTAIICLLAFWLPASPFAHALGLTALPFGLNVAIL 599 TL+VH+IRT +IPF QS+ S PL+ T II + +P +P AH L ALP ++ Sbjct: 837 TLIVHMIRTRRIPFFQSRASWPLMGMTLIIMAIGIAIPMTPLAHYFKLEALPLSYFPWLV 896 Query: 600 AIVFAYLLLTQLLKSWLISRFGLK 623 I+ AY +L Q +K W R+G + Sbjct: 897 GILVAYAVLIQGMKGWYAKRYGWQ 920 >gi|300311691|ref|YP_003775783.1| cation transport ATPase [Herbaspirillum seropedicae SmR1] Length = 853 Score = 209 bits (533), Expect = 4e-55, Method: Compositional matrix adjust. Identities = 162/550 (29%), Positives = 275/550 (50%), Gaps = 66/550 (12%) Query: 2 TSFDRGIDKYVHLLMRIMLVMVPLVFLLNGITKGDWLEALLFAVAIAVGLAPEMLPMIIT 61 + R I + V +L L++ L+ +L G + G W EALL +A+++ L PE LP+I+T Sbjct: 224 SPMQRHIARIVTVLAVTGLMLSALLVVLEGYSHGRWTEALLMGIALSMSLLPEELPIILT 283 Query: 62 INLAKGALAMSEKKVIVKRLNAIQNFGAMNILCTDKTGTLTQDR------IILEKHVDID 115 I A GA M+ V+ +RL+AI+ G++++LCTDKTGTLT++R +I +H+ D Sbjct: 284 IFPAIGAWRMARANVLTRRLSAIETLGSISVLCTDKTGTLTENRMRVVQGMIAGRHMYFD 343 Query: 116 ----GVDS---QKVVEYAYLNSFFQTGLKNLLDMAILSYPGMQEK-VEPDRHFHKIDELP 167 G+ S + ++E A L S + + ++ A G K P + E P Sbjct: 344 ESDIGITSPEWRTLLETAALASSPRP--TDPMEKAFHQLAGESAKRFAPPALTQLLQEYP 401 Query: 168 FDFERRRMSVIVEQANHTRLLIC-KGAPEEILSICTDAEHNGKAVPLAPHHGAELSKTVD 226 F R M+++ + + LL+ KGAPE I S+C L P ++ + Sbjct: 402 FTPALRAMTMVWKGDDDAPLLVATKGAPEAIASLCG----------LTPAEVQQMQNDIM 451 Query: 227 DLNDDGFRVIAVACKELPENGATATNEFSNNDESGLTLVGYIAFLDPPKDSAAEAIASLH 286 L G RVIAVA +GA ++ + GL +G +A DP + +AI H Sbjct: 452 HLGSHGLRVIAVAACNW--SGALPASQ----AQFGLRYLGLLALADPLRPGVPDAIQRCH 505 Query: 287 AYGVTVKVLSGDNGAVVRNVCRHVGLPGEQAVLGSDIDALSDEAVGELAERIHIFAKLNP 346 + V +++GD+ + +N+ R G+ +Q +LG ++ A+++ + ++ A++ P Sbjct: 506 QAEIRVIMITGDHPSTAQNIARQAGISADQVLLGDEVQAMAEHELARRLRTTNVCARITP 565 Query: 347 QQKARIIRCLQQRHHVVGYLGDGINDGAALKTADVGISVDS-AVDIAKESADIILMRKSL 405 QK RI+ L+ ++ GDG+ND ALK A VGI++ + D+A+E+ +IL+ + Sbjct: 566 LQKLRIVAALKDDGEIIAMTGDGVNDAPALKAAHVGIAMGARGTDVAREAGSLILLDDNF 625 Query: 406 VVLKDGVLEGRKVFGNITKYIKMSSSSAFGNMLSVL------GASAILPF-----LPMAP 454 + +G+L GR++F + G M+ VL A+LP L + P Sbjct: 626 ASIVEGILMGRRIFSRMR-----------GAMIYVLAMHMPIAGMALLPVVFGWPLILYP 674 Query: 455 VQILLNNLLYDFSQTTIATDHVDAEYLKE--PRRW---EILDIGRFMMVL--GPVSSLFD 507 +QI L+ D T+A ++ ++Y + PRR +LD ++VL G V+ LF Sbjct: 675 MQIAFLELIVD-PACTLAFENTGSDYRERQAPRRHGAAPLLDARIIILVLVQGSVALLFA 733 Query: 508 YLTFGVLWYL 517 + LW+L Sbjct: 734 VSAY--LWFL 741 >gi|300310937|ref|YP_003775029.1| heavy metal translocating P-type ATPase [Herbaspirillum seropedicae SmR1] Length = 744 Score = 78.2 bits (191), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 54/188 (28%), Positives = 88/188 (46%), Gaps = 27/188 (14%) Query: 240 CKELPENGATATNEFSNNDESGLTLVGYIAFLDPPKDSAAEAIASLHAYGVTVKVLSGDN 299 ++L G T + ND SG+ ++G +AF D K A AIA+LH GV +LSGDN Sbjct: 528 AEQLQAQGRTLSWLARRND-SGVEVLGLLAFGDQIKPGAQAAIATLHRMGVRSAMLSGDN 586 Query: 300 GAVVRNVCRHVGLPGEQAVLGSDIDALSDEAVGELAERIHIFAKLNPQQKARIIRCLQQR 359 + V +G+ + A++ P KA + L+Q Sbjct: 587 LGAAQQVASELGIQ-------------------------EVRAEVLPADKAEAVTALRQG 621 Query: 360 -HHVVGYLGDGINDGAALKTADVGISVDSAVDIAKESADIILMRKSLVVLKDGVLEGRKV 418 VV +GDG+ND AL ADVG+++ + D+A ++A + LM ++ D + R+ Sbjct: 622 GAQVVAMVGDGVNDAPALAAADVGMAMSTGTDVAMQAAGVTLMHGDPGLVADAIDISRRT 681 Query: 419 FGNITKYI 426 + I + + Sbjct: 682 YAKIRQNL 689 >gi|300310933|ref|YP_003775025.1| metal-transporting P-type ATPase transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 778 Score = 69.3 bits (168), Expect = 8e-13, Method: Compositional matrix adjust. Identities = 52/165 (31%), Positives = 78/165 (47%), Gaps = 28/165 (16%) Query: 268 IAFLDPPKDSAAEAIASLHAYGVTVKVLSGDNGAVVRNVCRHVGLPGEQAVLGSDIDALS 327 +A D + S+ +A+A L A GV V +L+GDN + + G+ DA Sbjct: 589 VAVADTVRPSSRQAVAELRALGVEVIMLTGDNSHTAQAIAAQTGIS----------DARG 638 Query: 328 DEAVGELAERIHIFAKLNPQQKARIIRCLQQRHHV--VGYLGDGINDGAALKTADVGISV 385 D+ P+ K + I L QRH V VG +GDGIND AL AD+G ++ Sbjct: 639 DQL---------------PEDKLKAIEELSQRHGVRGVGMVGDGINDAPALARADIGFAM 683 Query: 386 DSA-VDIAKESADIILMRKSLVVLKDGVLEGRKVFGNITKYIKMS 429 +A D A E+AD+ LM L + D + RK + + I ++ Sbjct: 684 GAAGTDTALETADVALMDDDLRKIPDFIRLSRKAHAVLVQNISIA 728 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.139 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,867,698 Number of extensions: 78315 Number of successful extensions: 315 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 308 Number of HSP's successfully gapped: 4 Length of query: 623 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 532 Effective length of database: 1,188,985 Effective search space: 632540020 Effective search space used: 632540020 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 133 (55.8 bits)