BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0185.0039.p (500 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311155|ref|YP_003775247.1| outer membrane drug efflux lipo... 468 e-133 gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbas... 388 e-109 gi|300310691|ref|YP_003774783.1| outer membrane drug efflux lipo... 361 e-101 gi|300313892|ref|YP_003777984.1| outer membrane drug efflux lipo... 336 3e-93 gi|300311271|ref|YP_003775363.1| outer membrane drug efflux lipo... 288 9e-79 >gi|300311155|ref|YP_003775247.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 491 Score = 468 bits (1205), Expect = e-133, Method: Compositional matrix adjust. Identities = 268/452 (59%), Positives = 337/452 (74%), Gaps = 12/452 (2%) Query: 39 CVSLAPDYVRPASPVPSHYDS--VTGASH-----APADIVGWRSYFQDPQLQSLIALALQ 91 C SLAP Y RPA+PV + Y V A+H A + + WR YF DP LQ+LI AL+ Sbjct: 25 CQSLAPHYQRPAAPVAAVYPDGPVNEAAHQVANEAASPPLPWRDYFTDPPLQALITQALE 84 Query: 92 NNRDLGIALARVDEARAAYGIERSAQFPSIGAQAELERSRVPGDLNLTGKPLVANQYQVG 151 N+RDL A RV EA+A YGI + FP+IGAQA L+RSR P DLNLT +PLV + YQ G Sbjct: 85 NSRDLRTAALRVQEAQALYGIGHADLFPTIGAQAGLDRSRTPADLNLTRRPLVGSAYQAG 144 Query: 152 LGMASWELDFWGHIRSLKDAALENYLASEAAQRAVRIDLIAQVATSYLILSELNERLFLA 211 LG++SWE+D WG +RSL DAALE+ AS+A + AV + L+AQVA ++L L+EL+ERL +A Sbjct: 145 LGLSSWEIDLWGRLRSLDDAALESARASQATRSAVALSLMAQVADAWLELAELDERLEIA 204 Query: 212 EQTLASRAESYRIFSRRVEVGSTSRLNLTQVAVLLTQAQSLVAQLEQERAAQVHRLTLLA 271 QT+ASR ES+RIFSRRVEVG+TSRLNLTQ+ LLTQAQ+L AQL+Q+RA +++ LTLL Sbjct: 205 NQTIASRQESFRIFSRRVEVGATSRLNLTQIETLLTQAQALGAQLQQQRAQRLNALTLLV 264 Query: 272 GTE--MDETTLRKRLSDVDAPRRLRAGAPSELLINRPDIIAAEHQLKAANANI-XARAAF 328 G+ + R L V AP L+ G P+ LL RPD+ AAEH+L AANANI ARAAF Sbjct: 265 GSPVVLPVGQARAGLDAVFAP--LQPGLPALLLTRRPDVAAAEHRLMAANANIGAARAAF 322 Query: 329 FPRIALTASAGTASAELGGLFGNGSRAWTFSPVISLPLFDGGLRNSNLNLTTARHHIAVL 388 FP I+LT + GTASAEL GLF +GS AWTFSP I+LP+F G +NL L+ R +AV Sbjct: 323 FPSISLTGNVGTASAELSGLFRDGSHAWTFSPSITLPIFTAGRLRNNLALSEVRRDLAVA 382 Query: 389 NYEKSIQNAFRDVSDALSARMWLDYQVDIASTSLAVQLERARLSTLRYDNGSASFLDVLD 448 YEK++Q AFR+V+DAL+AR WL Q++IA +++ VQ ERARLS LRYDNG+A FLDVLD Sbjct: 383 AYEKTVQTAFREVADALAARQWLTQQLEIAQSAVRVQRERARLSALRYDNGAAPFLDVLD 442 Query: 449 AQRDLLTAQQQLVQLQRALSTSNVSLYAALGG 480 AQRDLL+AQQQLVQ++RAL +S+V+LYAALGG Sbjct: 443 AQRDLLSAQQQLVQIRRALLSSSVALYAALGG 474 >gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 483 Score = 388 bits (997), Expect = e-109, Method: Compositional matrix adjust. Identities = 216/460 (46%), Positives = 300/460 (65%), Gaps = 2/460 (0%) Query: 27 FVAAVFVAQLAGCVSLAPDYVRPASPVPSHYDS-VTGASHAPADIVGWRSYFQDPQLQSL 85 AA+ +A + G SLAP Y RP +PV S Y + G + A +GWR +F D +LQ L Sbjct: 15 MCAALVMAGVLGGCSLAPTYERPQAPVESSYPADAAGRAELQAVNLGWRQFFPDQRLQQL 74 Query: 86 IALALQNNRDLGIALARVDEARAAYGIERSAQFPSIGAQAELERSRVPGDLNLTGKPLVA 145 IA AL+NNRDL A+ ++EARA Y I R+ P++ R+R +LTG+P ++ Sbjct: 75 IAAALENNRDLRTAVLNIEEARAQYQIARADLLPNLNLSGNAARTRTAASQSLTGQPTIS 134 Query: 146 NQYQVGLGMASWELDFWGHIRSLKDAALENYLASEAAQRAVRIDLIAQVATSYLILSELN 205 N YQVGL + ++ELDF+G +R+LKDAAL +YL++E AQ++ +I L+AQVA +YL Sbjct: 135 NSYQVGLAVPAYELDFFGRVRNLKDAALASYLSTEEAQKSAQISLVAQVAQAYLAERSYA 194 Query: 206 ERLFLAEQTLASRAESYRIFSRRVEVGSTSRLNLTQVAVLLTQAQSLVAQLEQERAAQVH 265 E+L LA++TL SR + + +R++VG+++ L+ L+ A+ QLE++RA + Sbjct: 195 EQLKLADETLKSRQSNLDLAQKRLDVGASTALDFRLTQTLVESARVAKTQLERQRAQAEN 254 Query: 266 RLTLLAGTEMDETTLRKRLSDVDAPRRLRAGAPSELLINRPDIIAAEHQLKAANANI-XA 324 LTLL G ++ E + LSD + AG PS+LL NRPDI +AE L AANANI A Sbjct: 255 ALTLLVGKKIGELPPAQLLSDEKIVTDIPAGLPSDLLTNRPDIRSAEQSLLAANANIGAA 314 Query: 325 RAAFFPRIALTASAGTASAELGGLFGNGSRAWTFSPVISLPLFDGGLRNSNLNLTTARHH 384 RAAFFPRI+LT + G+AS+ LGGLF +GS AW+F P +SLP+FD G ++NL+L R + Sbjct: 315 RAAFFPRISLTGNFGSASSTLGGLFESGSGAWSFGPSLSLPIFDFGRNSANLDLAEVRKN 374 Query: 385 IAVLNYEKSIQNAFRDVSDALSARMWLDYQVDIASTSLAVQLERARLSTLRYDNGSASFL 444 AV+ YEK+IQ AFR+V+DAL AR LD QV LA Q ER +L+ LR+ NG AS L Sbjct: 375 KAVVTYEKAIQTAFREVADALVARGSLDEQVKAQEAFLAAQQERLKLTDLRFKNGIASSL 434 Query: 445 DVLDAQRDLLTAQQQLVQLQRALSTSNVSLYAALGGATDE 484 D LDAQRDL +AQQ L+Q ++ + + LY ALGG +E Sbjct: 435 DQLDAQRDLFSAQQALIQARQLRLNNAIDLYKALGGGLNE 474 >gi|300310691|ref|YP_003774783.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 498 Score = 361 bits (926), Expect = e-101, Method: Compositional matrix adjust. Identities = 221/492 (44%), Positives = 301/492 (61%), Gaps = 35/492 (7%) Query: 31 VFVAQLAGCVSLAPDYVRPASPVPSHY-----DSVTGASHAP--ADIVGWRSYFQDPQLQ 83 V +A L G +L PDY RP +PV +H+ + GAS AP ADI GWR F DP LQ Sbjct: 18 VMLAMLLGGCTLMPDYHRPDAPVAAHFAGADSKNAAGASSAPVVADI-GWRDVFTDPSLQ 76 Query: 84 SLIALALQNNRDLGIALARVDEARAAYGIERSAQFPSIGAQAELERSRVPGDLNLTGKPL 143 +IALAL+NNRDL +A+ +++ARA YG++R+A FPSI A SR PGDL+ TG L Sbjct: 77 QVIALALENNRDLRVAVLNIEKARAQYGVQRAALFPSIKAATSETASRTPGDLSSTGSAL 136 Query: 144 VANQYQVGLGMASWELDFWGHIRSLKDAALENYLASEAAQRAVRIDLIAQVATSYLILSE 203 + Y LG +++ELD +G +RSLK+ AL+++L++ A+R+ +I LIA+VAT++L L+ Sbjct: 137 TSRSYTATLGFSAYELDLFGRVRSLKEQALQSFLSTAEARRSTQISLIAEVATAWLTLAS 196 Query: 204 LNERLFLAEQTLASRAESYRIFSRRVEVGSTSRLNLTQVAVLLTQA----QSLVAQLEQE 259 +RL LA+ TL S++ SY + R E+GS+S L L Q + A +S AQ++Q+ Sbjct: 197 DQDRLRLAQDTLESQSSSYALTQRSFELGSSSALTLRQAQTSVESARVDVESYTAQVDQD 256 Query: 260 RAAQVHRLTLLAGTEMDETTLRKRLSDVDAPRRLRA---------GAPSELLINRPDIIA 310 R A L LL G ++ L + L D RL A G PS+LL R DI+ Sbjct: 257 RNA----LVLLVGKDLPTALLPQGLPDT----RLAAASPLATIPPGLPSDLLQRRADIVQ 308 Query: 311 AEHQLKAANANI-XARAAFFPRIALTASAGTASAELGGLFGNGSRAWTFSPVISLPLFDG 369 AE L+AANANI ARAAF+P I LT +AGTASA L GLF GS +W+F P ISLP+FDG Sbjct: 309 AERDLRAANANIGAARAAFYPSITLTVNAGTASARLAGLFKGGSGSWSFVPQISLPIFDG 368 Query: 370 GLRNSNLNLTTARHHIAVLNYEKSIQNAFRDVSDALSARMWLDYQVDIASTSLAVQLERA 429 G +NL++ T I+V YEK+IQ AFR+VSDAL+ R L Q+ + E Sbjct: 369 GANQANLDIATVSRDISVAQYEKAIQTAFREVSDALAQRNTLGRQLQAQEALVQASDEAF 428 Query: 430 RLSTLRYDNGSASFLDVLDAQRDLLTAQQQLVQLQRALSTSNVSLYAALGGATDE---VP 486 RLS RY +G S+L VLD+QR L +AQ+ L+ Q + T+ V+ Y LGG E P Sbjct: 429 RLSQARYHSGVDSYLTVLDSQRTLYSAQKNLIGTQLSRWTNLVTFYKTLGGGWVERTGTP 488 Query: 487 AADGKTVSTTDP 498 AA ++T +P Sbjct: 489 AA--SDIATQEP 498 >gi|300313892|ref|YP_003777984.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 497 Score = 336 bits (861), Expect = 3e-93, Method: Compositional matrix adjust. Identities = 193/461 (41%), Positives = 284/461 (61%), Gaps = 15/461 (3%) Query: 38 GCVSLAPDYVRPASPVPSHYDSVTGASHA-----------PADIVGWRSYFQDPQLQSLI 86 GC +LAPDY +PA+PVP+ + S A A AD +GWR +F DP+LQ +I Sbjct: 27 GCANLAPDYTQPAAPVPASWTSQAAAQPAVGSAGPAAGEIDADHLGWREFFLDPRLQQVI 86 Query: 87 ALALQNNRDLGIALARVDEARAAYGIERSAQFPSIGAQAELERSRVPGDLNLTGKPLVAN 146 A AL NNRDL +A +++ARA Y I + ++PSI A SR P +L+ TG V++ Sbjct: 87 AAALDNNRDLRVAALNIEKARAQYRITDANRYPSISATGTSSASRTPAELSTTGSAAVSH 146 Query: 147 QYQVGLGMASWELDFWGHIRSLKDAALENYLASEAAQRAVRIDLIAQVATSYLILSELNE 206 Q V +G++++ELDF+G +++L DAALE+Y +R+ I L+A+VAT++L L+ + Sbjct: 147 QQSVTVGVSAYELDFFGRLKNLSDAALESYQYQVETRRSTHISLVAEVATAWLTLASDRD 206 Query: 207 RLFLAEQTLASRAESYRIFSRRVEVGSTSRLNLTQVAVLLTQAQSLVAQLEQERAAQVHR 266 L LA+ T AS++ ++ + R ++G+ S ++L + A++ VA+ + ++ Sbjct: 207 LLKLAQDTYASQSRTFALSQRSHDIGTVSGVDLASQEATVESARADVARYASQVEQDLNA 266 Query: 267 LTLLAGTEMDETTLRKRLSDVDA--PRRLRAGAPSELLINRPDIIAAEHQLKAANANI-X 323 L LL G ++D L L D + P AG PS++L+ RPD++AAEH LK+ANA+I Sbjct: 267 LRLLVGADIDAALLPAGLPDTASVLP-ATPAGLPSQVLLRRPDVLAAEHTLKSANADIGA 325 Query: 324 ARAAFFPRIALTASAGTASAELGGLFGNGSRAWTFSPVISLPLFDGGLRNSNLNLTTARH 383 ARAAFFP I+LTA+ GTAS LGGLF GS +WTFSP I+LP+F+ G ++L+ Sbjct: 326 ARAAFFPSISLTANGGTASRTLGGLFKAGSGSWTFSPSINLPIFNAGSLRASLDSAKITR 385 Query: 384 HIAVLNYEKSIQNAFRDVSDALSARMWLDYQVDIASTSLAVQLERARLSTLRYDNGSASF 443 I V NYEK+IQ AFR+V+DAL+ R LD ++D + A + RLS RY NG S+ Sbjct: 386 DIDVANYEKAIQTAFREVADALAVRATLDQRLDAQGKATAASEKAFRLSDARYRNGVDSY 445 Query: 444 LDVLDAQRDLLTAQQQLVQLQRALSTSNVSLYAALGGATDE 484 L L AQR L +AQQ L+ L+ ++ ++LY LGG E Sbjct: 446 LTTLVAQRTLYSAQQTLISLRLTEQSNRITLYKVLGGGWSE 486 >gi|300311271|ref|YP_003775363.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 481 Score = 288 bits (736), Expect = 9e-79, Method: Compositional matrix adjust. Identities = 191/460 (41%), Positives = 262/460 (56%), Gaps = 8/460 (1%) Query: 36 LAGCVSLAPDYVRPASPVPSHYDSVTGASHAPADI------VGWRSYFQDPQLQSLIALA 89 L C L A +P+HY T A APA+ +GW+ YF LQ+LIA A Sbjct: 17 LCACAPLTAPLPPSALDLPTHYPR-TLAEDAPANAAPTASAIGWQDYFPQAPLQALIAEA 75 Query: 90 LQNNRDLGIALARVDEARAAYGIERSAQFPSIGAQAELERSRVPGDLNLTGKPLVANQYQ 149 L++N DL R+ + +A + I+RS P++ +A+ RSR P + +G +Y+ Sbjct: 76 LRHNPDLRDIRLRLQQVQAQWRIQRSTGLPTVNLEAQARRSRGPLEPPESGPAASTREYR 135 Query: 150 VGLGMASWELDFWGHIRSLKDAALENYLASEAAQRAVRIDLIAQVATSYLILSELNERLF 209 VGLG +SWELDFWG I SL +AAL+ ++AS AA++A+ I LI QVA ++ L EL+ERL Sbjct: 136 VGLGASSWELDFWGRIASLNEAALQEWMASRAAEQALVISLIGQVAAAWTSLRELDERLH 195 Query: 210 LAEQTLASRAESYRIFSRRVEVGSTSRLNLTQVAVLLTQAQSLVAQLEQERAAQVHRLTL 269 L E A+ AES + R + G L Q L Q ++ A+L+Q RA+Q L + Sbjct: 196 LLETLQAATAESLALMQARHDAGRADAAALAQQRSELAQMRAEQAELQQMRASQRSTLEM 255 Query: 270 LAGTEMDETTLRKRLSDVDAPRRLRAGAPSELLINRPDIIAAEHQLK-AANANIXARAAF 328 LAG L + + P L G PS LL RPDIIAAEH+L+ +A ARAAF Sbjct: 256 LAGLTQLPAALEPAPTTILLPFALAPGLPSTLLAARPDIIAAEHRLQASAAQVAAARAAF 315 Query: 329 FPRIALTASAGTASAELGGLFGNGSRAWTFSPVISLPLFDGGLRNSNLNLTTARHHIAVL 388 FPRI+LTA G AS L LF SRAW F P ISLPL DGGLR S ++ A+L Sbjct: 316 FPRISLTAFGGVASTALQDLFKGPSRAWNFVPEISLPLLDGGLRQSRYDIAALDQQRALL 375 Query: 389 NYEKSIQNAFRDVSDALSARMWLDYQVDIASTSLAVQLERARLSTLRYDNGSASFLDVLD 448 +YE+++QNAFR+V+ L WL Q+ + + Q +R +R G AS LDVL+ Sbjct: 376 DYERTVQNAFREVAQRLEEAHWLKEQLRLRREVVQQQAQRQAQMHMRLRYGRASRLDVLE 435 Query: 449 AQRDLLTAQQQLVQLQRALSTSNVSLYAALGGATDEVPAA 488 ++RD L AQQ+ L+ AL + ++LY ALGG T + A+ Sbjct: 436 SERDHLRAQQRQHMLEHALLRNQIALYVALGGGTQALSAS 475 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.130 0.363 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,364,433 Number of extensions: 52022 Number of successful extensions: 262 Number of sequences better than 1.0e-08: 17 Number of HSP's gapped: 195 Number of HSP's successfully gapped: 17 Length of query: 500 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 411 Effective length of database: 1,198,455 Effective search space: 492565005 Effective search space used: 492565005 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)