BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0185.0022.p (149 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311835|ref|YP_003775927.1| AraC family ethanolamine operon... 116 9e-28 >gi|300311835|ref|YP_003775927.1| AraC family ethanolamine operon transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 332 Score = 116 bits (290), Expect = 9e-28, Method: Compositional matrix adjust. Identities = 63/120 (52%), Positives = 81/120 (67%), Gaps = 4/120 (3%) Query: 1 MIMTQPGHREFELMTPDDHAILGIVIQRNTLLAAADRLGCRIEWSRLASAELLHVGQVAL 60 ++MT+PG EFEL+TPD H I GIV+QR+ L A+A RLGC+ +W RL AELL V L Sbjct: 106 VVMTRPGACEFELVTPDAHEIYGIVVQRDALQASAARLGCQPDWQRLGQAELLKVDHGGL 165 Query: 61 DTCAQHIQTLLDPAT-PHGAHA---MQQDQVVTTLLAMLDASEVESEACTSLLRRRKIVS 116 + C Q I LL A GAHA ++Q+ V+ LLAMLD E+E+EA S LRRR+IV+ Sbjct: 166 EDCRQQIAALLAMAGQASGAHAALQLRQEAVLVALLAMLDEGEIEAEAGASFLRRRRIVN 225 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.324 0.130 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 388,191 Number of extensions: 12840 Number of successful extensions: 61 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 60 Number of HSP's successfully gapped: 1 Length of query: 149 Length of database: 1,619,870 Length adjustment: 77 Effective length of query: 72 Effective length of database: 1,255,275 Effective search space: 90379800 Effective search space used: 90379800 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 126 (53.1 bits)