BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0185.0008.p (477 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311584|ref|YP_003775676.1| pyruvate dehydrogenase [Herbasp... 675 0.0 gi|300311064|ref|YP_003775156.1| acetolactate synthase isozyme I... 88 1e-18 gi|300311743|ref|YP_003775835.1| acetolactate synthase [Herbaspi... 74 3e-14 >gi|300311584|ref|YP_003775676.1| pyruvate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 564 Score = 675 bits (1741), Expect = 0.0, Method: Compositional matrix adjust. Identities = 327/474 (68%), Positives = 386/474 (81%), Gaps = 4/474 (0%) Query: 4 ILLISGGVPASRRGIDYLQENNPDLLFRDVASYTQTIIDPAQTVQVVRQAIAQAYHQRGV 63 +L ISGGVPA+ RG DYLQEN+P+LLFRDVA+Y+Q I P Q V QAIAQAY QRGV Sbjct: 95 VLAISGGVPAAHRGTDYLQENSPELLFRDVAAYSQIITSPDQAPAVFHQAIAQAYAQRGV 154 Query: 64 AHISIPADVIGAKLPGPAAVASLNALRPQPEVAPPESEITQAAQRIDAAASVAIFVGNGA 123 AH++IPADVIGAK G +V SL+ LR + ++PP ++I AA I+AA SVAIF GNG Sbjct: 155 AHLNIPADVIGAKTSG--SVPSLHTLRERTPLSPPAADIQAAADLINAAGSVAIFAGNGC 212 Query: 124 RSAIGDIAVLAEKLQAPILHTFRAKDMLPYDHPHWIGGVGLIGGAPGMDAMRDAELLLML 183 R AI ++ LA KLQAP++HTFR KD+ YDHPHWIGGVGLIGGAPG DA+ AE++LML Sbjct: 213 RGAIDEVLALASKLQAPVMHTFRGKDLASYDHPHWIGGVGLIGGAPGTDALHAAEVMLML 272 Query: 184 GTDYPYSEFLPTRTQTVQIDERGFVLGRRMPVDLGITGSVGPAVTQLLQQVQGKTDTTFL 243 G+DYPYSEFLPT+++ +QIDERGFVLGRR PV LGITGSV PAV QLL++V+GKTD FL Sbjct: 273 GSDYPYSEFLPTKSKVIQIDERGFVLGRRAPVTLGITGSVAPAVQQLLERVEGKTDGAFL 332 Query: 244 RKVGEHRKQWNKTLEKHAIAPPDPDKGPIKPQYLARRLSDRAAEDAVFVVDTGVVTLWCG 303 ++ HR++WN L+ A K IKPQ +AR +SD A +DA FVVDTGVVTLWCG Sbjct: 333 QQANAHRERWNAKLDSAAEIKRHHKK--IKPQSVARAISDLADDDAAFVVDTGVVTLWCG 390 Query: 304 NWIRQSGRQRLLASFNNAAVGTSLGQGNGIQALDRNRQVIVAAGDGGFTMLLGEFMTAVE 363 NWIRQ G QR+LASFNNAAVGTSLGQ NGIQALDR RQV+VA GDGGFTMLLGEFMT+VE Sbjct: 391 NWIRQRGGQRILASFNNAAVGTSLGQANGIQALDRERQVVVAVGDGGFTMLLGEFMTSVE 450 Query: 364 HKLPVKVVVFNNREWGLVHLEMEEAGLPAFQGSEFPNLDFALFAGACGAQGFTAKTPAQL 423 H+LPVKVVVFNNREWGLVHLEMEEAG+PAF+GSEFPN DFA FA ACG QGF+ +TP +L Sbjct: 451 HRLPVKVVVFNNREWGLVHLEMEEAGMPAFEGSEFPNPDFAAFARACGGQGFSVRTPEEL 510 Query: 424 EEALGPFLAAPGPAILNVFIDPSELPIMPHIKLDQIWHFGMAKVKEAMISMGGG 477 E L LAAPGPA+L+VFIDP ELP+MPHIK++QIW FG+AKV+E++I+M GG Sbjct: 511 EGGLQQLLAAPGPAVLDVFIDPDELPVMPHIKMEQIWRFGLAKVRESLIAMKGG 564 >gi|300311064|ref|YP_003775156.1| acetolactate synthase isozyme III large subunit [Herbaspirillum seropedicae SmR1] Length = 572 Score = 88.2 bits (217), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 102/467 (21%), Positives = 184/467 (39%), Gaps = 22/467 (4%) Query: 4 ILLISGGVPASRRGIDYLQENNPDLLFRDVASYTQTIIDPAQTVQVVRQAIAQAYHQR-G 62 +++ISG VP++ G D QE + + R + + D + V++A A R G Sbjct: 98 MVVISGQVPSTAIGQDAFQECDTVGITRPCVKHNFLVKDVKDLAETVKKAFYIATTGRPG 157 Query: 63 VAHISIPADVI--GAKLPGPAAVASLNALRPQPEVAPPESEITQAAQRIDAAASVAIFVG 120 + IP D+ P AV + + +P + +I +A Q + +A I+ G Sbjct: 158 PVLVDIPKDITMHTCAYAYPKAV-EMRSYKPVDK--GHSGQIRKALQLLLSAERPMIYTG 214 Query: 121 NGA--RSAIGDIAVLAEKLQAPILHTFRAKDMLPYDHPHWIGGVGLIGGAPGMDAMRDAE 178 G +A ++ L ++L P +T ++G G+ G AM+ + Sbjct: 215 GGIILANAAPELNKLVDRLGYPCTNTLMGLGAYRSSSDKFVGMPGMHGTYEANMAMQHCD 274 Query: 179 LLLMLGTDYPY------SEFLPTRTQTVQIDERGFVLGRRMPVDLGITGSVGPAVTQLLQ 232 +L+ +G + F + + ID + +R+ VD+ I G+V + +LL Sbjct: 275 VLIAIGARFDDRVIGNPKHFASHARKIIHIDIDPSSISKRVKVDIPIVGNVKDVLQELLS 334 Query: 233 QVQGKTDTTFLRKVGEHRKQWNKTLE---KHAIAPPDPDKGPIKPQYLARRLSDRAAEDA 289 Q+ D R+ G W K + K + IKPQ + +++ + DA Sbjct: 335 QL----DAAETRQNGAALDAWWKQINEWRKRDCLKFATSEEFIKPQSVVQKVWEVTKGDA 390 Query: 290 VFVVDTGVVTLWCGNWIRQSGRQRLLASFNNAAVGTSLGQGNGIQALDRNRQVIVAAGDG 349 D G +W + +R + S +G L G+Q + + V G+ Sbjct: 391 FITSDVGQHQMWAAQYYGFDKPRRWINSGGLGTMGVGLPYAMGVQMANPDATVACITGEA 450 Query: 350 GFTMLLGEFMTAVEHKLPVKVVVFNNREWGLVHLEMEEAGLPAFQGSEFPNL-DFALFAG 408 M + E T ++ L K+++ NNR G+V + + S +L DF A Sbjct: 451 SIQMCIQELATCKQYHLTPKIILLNNRFLGMVRQWQQIDYGSRYSESYMDSLPDFNKLAE 510 Query: 409 ACGAQGFTAKTPAQLEEALGPFLAAPGPAILNVFIDPSELPIMPHIK 455 + G G + P ++ AL A + FI + P +K Sbjct: 511 SYGHVGMKIEKPGDVDGALREAFAMKDRLVFMNFITDQTENVWPMVK 557 >gi|300311743|ref|YP_003775835.1| acetolactate synthase [Herbaspirillum seropedicae SmR1] Length = 625 Score = 73.9 bits (180), Expect = 3e-14, Method: Compositional matrix adjust. Identities = 112/479 (23%), Positives = 193/479 (40%), Gaps = 45/479 (9%) Query: 4 ILLISGGVPASRRGIDYLQE----NNPDL----LFRDVASYTQTIIDPAQTVQVVRQAIA 55 +LL+ G V SRR LQ+ N+P L FR ++ I P Q + + +AI Sbjct: 119 VLLLPGDVFVSRRPDPVLQQLEDFNDPGLSVNDAFRPLSRMFDRICYPEQLLTALPRAIQ 178 Query: 56 QAYH--QRGVAHISIPADVIGAKLPGPAAVASLNALRPQPEVAPPESEITQAAQRIDAAA 113 Q G +++P DV P S + P+ + AP E+ A + A Sbjct: 179 VLTDPAQCGPVTLALPQDVQTMAYDYPLDFFSPRVIVPRAQ-APAAQELEDAVALLRQAR 237 Query: 114 SVAIFVGNGAR--SAIGDIAVLAEKLQAPILHTFRAKDMLPYDHPHWIGGVGLIGGAPGM 171 + G G +A + AE+ P+ T K LP+ H +G +G + G+P Sbjct: 238 QPLLIAGGGVLYGNACEHLRRFAEQHGVPVAETQAGKSALPWSHALQMGAIG-VTGSPAA 296 Query: 172 DAM-RDAELLLMLGT---DY---PYSEFLPTRTQTVQIDERGFVLGRRMPVDLGITGSVG 224 +A+ ++A++++ +GT D+ ++ F R + ++ R L + G Sbjct: 297 NALAQEADVVIAVGTRLQDFTTGSHTLFAQARLLNLNVNAMDAHKWR----GLALQADAG 352 Query: 225 PAVTQLLQQVQG-KTDTTFLRKVGEHRKQWNKTLEKHAIAPPDPDKGPIKPQ------YL 277 + L ++G ++ + + + W E+ A D G P + Sbjct: 353 LGLEALSHALEGWRSAPEWHARAHTLAQDWR---ERVAQITGQTDTGGRLPYDGEVIGAI 409 Query: 278 ARRLSDRAAEDAVFVVDTGVVTLWCGNWIRQSGRQRLLASFNNAAVGTSLGQGNGIQALD 337 R ++D ++D + V G + R + + +G + G G++ Sbjct: 410 QRSVADSPSQD-IVVCAAGTLPAELHKLWRAGRPGAYHVEYGYSCMGYEVAGGLGVKLAQ 468 Query: 338 RNRQVIVAAGDGGFTMLLGEFMTAVEHKLPVKVVVFNNREWGLVHLEMEEAGLPAFQ--- 394 R+VIV GDG + M+ E T+V + VVV +NR +G ++ + G F Sbjct: 469 PQREVIVIVGDGSYLMMNSELATSVMLGAKLIVVVLDNRGYGCINRLQQACGGAPFNNML 528 Query: 395 ------GSEFPNLDFALFAGACGAQGFTAKTPAQLEEALGPFLAAPGPAILNVFIDPSE 447 G P +DFA A + GA G KT +LE AL AA ++ + D S Sbjct: 529 ADCLQAGPGAPAIDFAAHARSLGALGENVKTITELEAALQRARAADRSYLVCIDTDASR 587 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.137 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,530,643 Number of extensions: 66046 Number of successful extensions: 262 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 258 Number of HSP's successfully gapped: 3 Length of query: 477 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 388 Effective length of database: 1,198,455 Effective search space: 465000540 Effective search space used: 465000540 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)