BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0184.0004.p (138 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311075|ref|YP_003775167.1| methyltransferase [Herbaspirill... 135 2e-33 gi|300312539|ref|YP_003776631.1| methyltransferase [Herbaspirill... 79 9e-17 >gi|300311075|ref|YP_003775167.1| methyltransferase [Herbaspirillum seropedicae SmR1] Length = 448 Score = 135 bits (339), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 66/123 (53%), Positives = 86/123 (69%) Query: 16 LLQQAIGFHTSGDLPRAERIYREVLAQDPVHPIALHYLGIFLHQNGQHDEGVQSIRLSCA 75 LL+QAI H +G L +A IY VL DP+HP+ALHY GI+LHQ+GQHDE + + L+ A Sbjct: 21 LLRQAIDAHMAGRLDQACAIYEAVLEADPIHPLALHYYGIWLHQDGQHDEALDKLELASA 80 Query: 76 LQPDNAAWHNDLGNVLFALREFEEASEAYQASLDADPHDHVVWNNLGSSQLQHNDTEGAI 135 L+PDNA WHND GNVLFAL++ A +AY +L P DH +WNNLG+ LQ + AI Sbjct: 81 LEPDNADWHNDRGNVLFALQQPALAEQAYADALALRPDDHTIWNNLGAVLLQQDKRPDAI 140 Query: 136 ASF 138 ++F Sbjct: 141 SAF 143 >gi|300312539|ref|YP_003776631.1| methyltransferase [Herbaspirillum seropedicae SmR1] Length = 439 Score = 79.3 bits (194), Expect = 9e-17, Method: Compositional matrix adjust. Identities = 49/111 (44%), Positives = 67/111 (60%) Query: 27 GDLPRAERIYREVLAQDPVHPIALHYLGIFLHQNGQHDEGVQSIRLSCALQPDNAAWHND 86 G L AE YR LA + H HYLG+ LHQ G+H+EGV +R + P +A W ND Sbjct: 27 GRLEEAEEDYRVTLAMEEHHAPTQHYLGMLLHQRGRHEEGVTLMRQAALAAPADADWCND 86 Query: 87 LGNVLFALREFEEASEAYQASLDADPHDHVVWNNLGSSQLQHNDTEGAIAS 137 +GNVLFA F+EA++AYQ +L P D +W NLG+++ + AIA+ Sbjct: 87 IGNVLFACSRFDEAAQAYQQALALRPDDAQLWTNLGATRRAQGQHQAAIAA 137 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.133 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 407,161 Number of extensions: 15443 Number of successful extensions: 74 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 74 Number of HSP's successfully gapped: 2 Length of query: 138 Length of database: 1,619,870 Length adjustment: 76 Effective length of query: 62 Effective length of database: 1,260,010 Effective search space: 78120620 Effective search space used: 78120620 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 126 (53.1 bits)