BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0183.0024.p (335 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312451|ref|YP_003776543.1| D-galactonate transporter prote... 107 1e-24 gi|300309918|ref|YP_003774010.1| major facilitator superfamily p... 104 8e-24 gi|300313884|ref|YP_003777976.1| phthalate permease of the major... 103 2e-23 gi|300309866|ref|YP_003773958.1| major facilitator superfamily p... 87 2e-18 gi|300313393|ref|YP_003777485.1| major facilitator superfamily p... 84 2e-17 >gi|300312451|ref|YP_003776543.1| D-galactonate transporter protein [Herbaspirillum seropedicae SmR1] Length = 439 Score = 107 bits (268), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 80/326 (24%), Positives = 149/326 (45%), Gaps = 25/326 (7%) Query: 2 IASTSAPPRPXLARLLLGLGEGATFPAATTAMSRWVAKDKRGFAQGITHAFARIGNAVAP 61 +++ +A L R ++GL E +FPA ++ W +RG A I ++ + Sbjct: 102 MSTAAAVASLFLLRFMVGLAEAPSFPANGRIVAAWFPTKERGTASAIFNSAQYFAAVLFT 161 Query: 62 AAVVWIMAEHGWRESFYIFGAFSLVWVIVWAFVFTEDPVRHPRITPEELAILPPG----- 116 + WI+ + W F + G + ++W + P HPRI ELA + G Sbjct: 162 PLMAWIVHTYSWHHVFTVMGVVGIALALIWGRII-YSPKDHPRINQAELAHIESGGGLVD 220 Query: 117 ----KKKSPQIPWGPLFKRMMP------VTIVYFCYGWTLWLFLSWIPQYFLHSYDLDIK 166 K +P ++++ V I +C + FL+W P Y + + I Sbjct: 221 MDNRKVAAPSTKGRGYIRQLLSNRMLLGVYIGQYCINVITYFFLTWFPVYLVQERGMSIL 280 Query: 167 KSALFASSVFFAGVVGDTLGGIVSDKILRSTGNLKRARSYMVSVCMLLTL-LSLLPLMFT 225 K+ AS G +G +GG++SD++++ ++ AR + MLL++ + L + Sbjct: 281 KAGFVASIPAVCGFIGGVVGGLISDRLIKRGLSVTWARKIPIVGGMLLSMTMILCNYVEA 340 Query: 226 HNLYVSIISLSAGFFFAEMTIGPMWAIPMDIAP-EYSGTASGMMNTGSALAAIISPVLSG 284 + V I++L+ FF + WA+ D AP + G + G+ N +A I +P++ G Sbjct: 341 QWMVVGIMALA---FFGKGVGALGWAVVADTAPKQIVGLSGGLFNMFGNVAGITTPIVIG 397 Query: 285 YLIDKTGNWELPFLGSMVLMGFGVLL 310 Y++ +TG+ F G++V +G L+ Sbjct: 398 YIVKETGS----FSGALVFVGINALV 419 >gi|300309918|ref|YP_003774010.1| major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 428 Score = 104 bits (260), Expect = 8e-24, Method: Compositional matrix adjust. Identities = 74/288 (25%), Positives = 127/288 (44%), Gaps = 12/288 (4%) Query: 13 LARLLLGLGEGATFPAATTAMSRWVAKDKRGFAQGITHAFARIGNAVAPAAVVWIMAEHG 72 +AR+ LGLGE FPA +S W A +RG GI A + IG A+AP + +M G Sbjct: 110 VARVFLGLGEAPQFPAGAKVVSEWFAMRERGGPTGIFVASSTIGPALAPPILTILMLSFG 169 Query: 73 WRESFYIFGAFSLVWVIVWAFVFTEDPVRHPRITPEELAILPPGKKKSPQI-------PW 125 WR F I G + + W + ++TP E+ L P W Sbjct: 170 WRMMFVIMGVLGIAVSVGWYLFYRNR--NEVQLTPSEVLYLADDHDNDPNANAKLSWSEW 227 Query: 126 GPLFKRMMPVTIVYFCYG--WTLWLFLSWIPQYFLHSYDLDIKKSALFASSVFFAGVVGD 183 LF +++ G + +WL+L+W+P Y H L I ++ + G +G Sbjct: 228 KSLFTYRTTWGMLFGFMGVIYMVWLYLTWLPAYLEHERHLSIARTGWVVVIPYIFGTLGM 287 Query: 184 TLGGIVSDKILRSTGNLKRARSYMVSVCMLLTLLSLLPLMFTHNLYVSIISLSAGFFFAE 243 G ++D +++ R+R + + ++ +P +T + ++I+ +S F Sbjct: 288 LSSGYIADGLMKRGMPAIRSRKWPICAGLIGAGAFTIPAAYTPSTTMAIVYISLAMAFVN 347 Query: 244 MTIGPMWA-IPMDIAPEYSGTASGMMNTGSALAAIISPVLSGYLIDKT 290 M G WA + + I + M N G L ++PV++G ++D+T Sbjct: 348 MASGGAWALVSVAIPRRLVASLGSMQNFGGYLGGSLAPVITGVVVDQT 395 >gi|300313884|ref|YP_003777976.1| phthalate permease of the major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 418 Score = 103 bits (257), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 83/287 (28%), Positives = 134/287 (46%), Gaps = 14/287 (4%) Query: 15 RLLLGLGEGATFPAATTAMSRWVAKDKRGFAQGITHAFARIGNAVAPAAVVWIMAEHGWR 74 R LLG+ E A P A + W +R A G + + IG AP VVW + GW+ Sbjct: 105 RALLGITEAAGIPGGVKASTEWFPAKERSVAIGWFNIGSSIGALCAPPLVVWTILHGGWK 164 Query: 75 ESFYIFGAFSLVWVIVWAFVFTEDPVRHPRITPEELAILPPGKKKSPQI----PWGPLF- 129 SF + GA ++W ++W +F + P ++PEE A + G++KSP+ W + Sbjct: 165 MSFVVVGALGVIWFVLW-MLFYKSPRDQKLLSPEERAYILEGQEKSPEKVQRESWTKIVR 223 Query: 130 -KRMMPVTIVYFCYGWTLWLFLSWIPQYFLHSYDLDIKKSALFASSVFFAGVVGDTLGGI 188 + + I F F +WIP Y ++IK+ A+FA F A +G LGG Sbjct: 224 SRNFWSIAIPRFLSEPAWQTFNAWIPLYMATERHMNIKEIAMFAWLPFLAADIGCVLGGY 283 Query: 189 VSDKILRSTGNLKRARSYMVSVCMLLTLLSLL---PLMFTHNLYVSIISLSAGFFFAEMT 245 +S + +LK + + ML+ LS++ + F + YV+I LS G F + Sbjct: 284 LSPLFHK---HLKVSLFTSRKLVMLVGSLSMIGPACVGFVDSPYVAIALLSIGGFAHQTL 340 Query: 246 IGPMWAIPMDI-APEYSGTASGMMNTGSALAAIISPVLSGYLIDKTG 291 G +++I D+ TA+G+ L A + +L G L+ + G Sbjct: 341 SGALYSITSDVFTKNQVATATGLTGMSGYLGATLFTLLFGILVTQIG 387 >gi|300309866|ref|YP_003773958.1| major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 484 Score = 87.0 bits (214), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 87/337 (25%), Positives = 147/337 (43%), Gaps = 49/337 (14%) Query: 1 LIASTSAPPRPXLARLLLGLGEGATFPAATTAMSRWVAKDKRGFAQGITHAFARIGNAVA 60 L+ +TS+ L R+ LGL EG T ++RW +++G A G+ A +++G + Sbjct: 129 LMGTTSSVLLLQLYRVGLGLSEGPLGIGCTNVINRWFPPEEKGTATGLWIAASKLGPLIV 188 Query: 61 PAAVVWIMAEHGWRESFYIFGAFSLVWVIVWAFVFTEDPVRH-------PRITPEELAIL 113 P + ++ GWRE FY+F + I+W F+ T P + R +++A Sbjct: 189 PTVCIIVIQLWGWREIFYVFAVPGIFLAILWFFLVTNSPSENRFCNEAERRYITQDVAAG 248 Query: 114 PPGKKKSPQ---------IPW--GPLFKRMMP----------------VTIVYFCYGWTL 146 P S + +PW G R +P V I Y C Sbjct: 249 PATATASSRAHVGAQVTPMPWLDGINRTRRVPRLETLRQVFTSWNVIGVAIGYGCMIGIS 308 Query: 147 WLFLSWIPQYFLHSYDLDIKKSALFASSVFFAGVVGDTLGGIVSDKILRSTGNLKRARSY 206 +F+SWIP Y + K AS+ F V G+ LGG++SD++L R Sbjct: 309 NIFMSWIPTYLVTVKGFASVKMGFLASAPFIGAVAGNMLGGVISDRLLGGR------RKP 362 Query: 207 MVSVCMLLTLLSLLPLMFTHNLYVSIISLSAGFFFAEMTIGPMW----AIPMDIAPEYSG 262 M+ + L T L L L+ + S++ L A + + +G + A PM +A + + Sbjct: 363 MMLLGALGTALMTLLLVEAPD---SVLYLGAMLMLSGLMLGIGFAGYSAYPMGLATKATY 419 Query: 263 TAS-GMMNTGSALAAIISPVLSGYLIDKTGNWELPFL 298 A+ G++N+ + +P+ G L+D+ +W FL Sbjct: 420 PAAFGIVNSLGQIGGACAPLAVGMLLDRY-DWSSVFL 455 >gi|300313393|ref|YP_003777485.1| major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 428 Score = 84.0 bits (206), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 85/321 (26%), Positives = 138/321 (42%), Gaps = 19/321 (5%) Query: 15 RLLLGLGEGATFPAATTAMSRWVAKDKRGFAQGITHAFARIGNAVAPAAVVWIMAEHGWR 74 R L+GL E A P+ A++ W ++ A G ++ +G +AP V+++ +GW+ Sbjct: 105 RALMGLTEAAAIPSGMKAVAEWFPDKEKSVAVGWFNSGTSLGAMLAPPLVIFLQIRYGWQ 164 Query: 75 ESFYIFGAFSLVWVIVWAFVFTEDPVRHPRITPEELAILPPGKKKSPQIPWGP------- 127 +F + G VW +W F F P H ++ E + G+ + G Sbjct: 165 SAFVVTGILGFVWAALW-FAFYRSPRDHAALSDSERETIISGQLRPVSAVRGKRPVKDVV 223 Query: 128 LFKRMMPVTIVYFCYGWTLWLFLSWIPQYFLHSYDLDIKKSALFASSVFFAGVVGDTLGG 187 +R + + F F WIP YF++ LD+K A+FA F A +G GG Sbjct: 224 RSRRFWAIALARFFAEPAWQTFSFWIPLYFVNERHLDLKSIAMFAWLPFLAADLGGLAGG 283 Query: 188 IVSDKILRSTGNLKRARSYMVSVC--MLLTLLSLLP--LMFTHNLYVSIISLSAGFFFAE 243 +S ++ N R VC +L ++ + P + + YV+I G F + Sbjct: 284 YLSPFLI----NRFRIPLVWSRVCGVVLGAVMMIGPACVGLVASPYVAIALFCVGGFAHQ 339 Query: 244 MTIGPMWAIPMDI-APEYSGTASGMMNTGSALAAIISPVLSGYLIDKTGNWEL-PFLGSM 301 M G + + D+ PE GTASG + + + +L G L D G L LG+ Sbjct: 340 MISGLVNTLSADVFDPEEVGTASGFAGMSAWIGGLGFSLLVGALADTVGYGPLFGMLGAF 399 Query: 302 VLMGFGVLLAFRMQPDSKFEN 322 L+G VLL ++ S E Sbjct: 400 DLIG-AVLLIILIRGQSTEER 419 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.139 0.444 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,048,545 Number of extensions: 43429 Number of successful extensions: 249 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 240 Number of HSP's successfully gapped: 11 Length of query: 335 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 249 Effective length of database: 1,212,660 Effective search space: 301952340 Effective search space used: 301952340 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 131 (55.1 bits)