BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0183.0021.p (242 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313121|ref|YP_003777213.1| glycolate oxidase (FAD-linked s... 57 2e-09 >gi|300313121|ref|YP_003777213.1| glycolate oxidase (FAD-linked subunit) oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 495 Score = 56.6 bits (135), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 28/78 (35%), Positives = 43/78 (55%) Query: 1 VIDFDPAARTITVEPGMVLDHLNAWLKPHGLWFPVDVSTAAQCTIGGMSGNNSCGSRSIE 60 ++ D +RT V+PG+ ++ PHGL++ D S+ C+IGG NS G ++ Sbjct: 108 ILQMDKYSRTAVVQPGVRNLAISEAAAPHGLYYAPDPSSQIACSIGGNVAENSGGVHCLK 167 Query: 61 YGNMVHNVLAIDAVLADG 78 YG VHNVL + V +G Sbjct: 168 YGLTVHNVLRVRMVTIEG 185 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.139 0.415 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 816,683 Number of extensions: 34089 Number of successful extensions: 92 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 94 Number of HSP's successfully gapped: 1 Length of query: 242 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 159 Effective length of database: 1,226,865 Effective search space: 195071535 Effective search space used: 195071535 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 129 (54.3 bits)