BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0183.0014.p (191 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311298|ref|YP_003775390.1| phenylalanyl-tRNA synthetase su... 256 6e-70 >gi|300311298|ref|YP_003775390.1| phenylalanyl-tRNA synthetase subunit alpha [Herbaspirillum seropedicae SmR1] Length = 372 Score = 256 bits (655), Expect = 6e-70, Method: Compositional matrix adjust. Identities = 123/130 (94%), Positives = 126/130 (96%), Gaps = 1/130 (0%) Query: 1 MQDTFYIEGNDTQGKPLLLRTHTSPMQVRYARMNKPPIKVIAPGRTYRVDSDATHSPMFH 60 MQDTFYIEGND+QGKPLLLRTHTSPMQVRYARMNKPPIKVIAPGRTYRVDSDATHSPMFH Sbjct: 188 MQDTFYIEGNDSQGKPLLLRTHTSPMQVRYARMNKPPIKVIAPGRTYRVDSDATHSPMFH 247 Query: 61 QVEGLWIAEDISFADLKGVYLNFVKAFFETDDLQVRFRPSYFPFTEPSAEIDIAFGSGPL 120 QVEGLWIAEDISFADLKGVYLNFVKAFFETDDLQVRFRPSYFPFTEPSAEIDIAFGSGPL Sbjct: 248 QVEGLWIAEDISFADLKGVYLNFVKAFFETDDLQVRFRPSYFPFTEPSAEIDIAFGSGPL 307 Query: 121 KGXF-SCSGS 129 KG + SG+ Sbjct: 308 KGRWLEVSGA 317 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.138 0.426 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 644,607 Number of extensions: 26871 Number of successful extensions: 53 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 53 Number of HSP's successfully gapped: 1 Length of query: 191 Length of database: 1,619,870 Length adjustment: 80 Effective length of query: 111 Effective length of database: 1,241,070 Effective search space: 137758770 Effective search space used: 137758770 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 128 (53.9 bits)