BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0183.0002.p (375 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311313|ref|YP_003775405.1| acyltransferase [Herbaspirillum... 147 1e-36 gi|300310014|ref|YP_003774106.1| o-antigen acetylase [Herbaspiri... 86 4e-18 gi|300311314|ref|YP_003775406.1| O-antigen acetylase [Herbaspiri... 82 5e-17 gi|300311522|ref|YP_003775614.1| O-antigen acetylase [Herbaspiri... 78 1e-15 gi|300310314|ref|YP_003774406.1| acyltransferase [Herbaspirillum... 75 6e-15 >gi|300311313|ref|YP_003775405.1| acyltransferase [Herbaspirillum seropedicae SmR1] Length = 350 Score = 147 bits (371), Expect = 1e-36, Method: Compositional matrix adjust. Identities = 129/309 (41%), Positives = 172/309 (55%), Gaps = 10/309 (3%) Query: 16 IPGLDGLRACAVLLVLVAHMGFSTSIPGGFGVTVFFFISGTLITRLLLAEHAQNGSIAVG 75 IP LDG RA A+ +V ++H G +PGG GVT+FFF+SG LIT LL+ E ++ S+ V Sbjct: 9 IPSLDGWRALAIAIVFLSHAGLGRIVPGGLGVTIFFFLSGYLITTLLVREFDRSDSVNVR 68 Query: 76 RFYVRRLFRLYPALLVAVALGALVFTLA--GGIMSWGKVFASLFYYANYYGIFVHFGQGR 133 FY+RRL RL P LL+ + L + L G SW A LFY+ANYY +F F G Sbjct: 69 HFYLRRLLRLTPPLLLVLVLTYTLTWLGIFTGDASWSGFLAQLFYFANYYVLF--FDPGH 126 Query: 134 DGFDPFSILWSLAVEEQYYLVFPFVCVALLAGAASLRRFAWVLAASVALVLIWRV-IVHH 192 + WSLAVEE +Y VFP + + L+ A R+ L LVL+WR +V Sbjct: 127 TLPQGTGVFWSLAVEEHFYFVFPALFIFLMH-KADRRKIPLYLGGLCLLVLLWRYGLVLL 185 Query: 193 SGGSSDYIYMATDTRIDSILYGAWLA-LILANDTQKHWRRFSSSHVVQAVCLLLLLVCFT 251 S D Y ATDTRIDSIL+G LA L L + + + +LLLL+ Sbjct: 186 GDASMDRTYYATDTRIDSILFGCILATLKLPPAVYAALHKPAVRVGGVTLGVLLLLLSLA 245 Query: 252 LRHPGFRDTLRYSLQGLALMPLVTAICFTDKLAPLTRLLESKPARRVGNWSYSLYLYHPI 311 +R FR+TLRYS+QGLALMPL P RLL + +R+G +SYS+YL H Sbjct: 246 VRDEVFRETLRYSVQGLALMPLFFYSVAHPHSFPF-RLLNLRWVQRIGIYSYSIYLSH-- 302 Query: 312 AIVIAEICW 320 I+++ + W Sbjct: 303 EILLSNVDW 311 >gi|300310014|ref|YP_003774106.1| o-antigen acetylase [Herbaspirillum seropedicae SmR1] Length = 681 Score = 86.3 bits (212), Expect = 4e-18, Method: Compositional matrix adjust. Identities = 116/382 (30%), Positives = 168/382 (43%), Gaps = 49/382 (12%) Query: 7 ASARPSFDYIPGLDGLRACAVLLVLVAHMGFSTSIPGGF-GVTVFFFISGTLITRLLLAE 65 A +P Y P +DGLRA A++ V+V H F ++PGGF GV +FF ISG LI+ ++ + Sbjct: 25 AQGKP-LSYRPDIDGLRAIAIVSVVVFH-AFPDAMPGGFIGVDIFFVISGFLISSIIFRQ 82 Query: 66 HAQNGSIAVGRFYVRRLFRLYPALLVAVA---------LGALVFTLAGGIMSWGKVFAS- 115 Q G + FYV R+ R++PAL++ +A L + G M+ G F Sbjct: 83 LDQ-GRFSFTDFYVHRIRRIFPALILVLASCFVLGWFSLLPEEYRQLGKHMAAGAGFIQN 141 Query: 116 --LFYYANYYGIFVHFGQGRDGFDPFSILWSLAVEEQYYLVFPFVCVALLAGAASLRR-F 172 L+ + Y+ + P LWSL+VEEQ+YLV+P + L LR Sbjct: 142 LVLWKESGYFDV-------ESDMKPLLHLWSLSVEEQFYLVYPLLVCGLWRARMPLRNSL 194 Query: 173 AWVLAASVALVLIWRVIVHHSGGSSDYIYMATDTRIDSILYGAWLAL-ILANDTQKHWRR 231 ++ AS AL L W V + Y+ TR+ +L GA A ++ Sbjct: 195 VLLMLASFALNLWW---VAQDPVKTFYLPF---TRVWELLAGALCAFGVMRKGVAFSTSS 248 Query: 232 FSSSHVVQAVCLLLLLVCFTLRHPGFRDTLRYSLQGLALMPLVTAICF--TDKLAPLTRL 289 F H+ +L + L D RY AL+P+ A+ + A + R Sbjct: 249 FRLPHLPSIHLRSVLGLALLLCGLVLIDRHRYFPGAWALLPVTGAVLLVSSGPQAWVNRH 308 Query: 290 LESKPARR-VGNWSYSLYLYHPIAIVIAEICWGPGSLGPQRIGWQTFPWFAATAVLLSFA 348 L S+P +G SY LYL+H + I L P R G AV LS Sbjct: 309 LLSRPLLVWIGLVSYLLYLWHWPILSFMHI------LTPTRPGAP----LPLAAVALSVL 358 Query: 349 LAVASYNFVEKPFLRWRRRFGA 370 LA +Y +E+P RFGA Sbjct: 359 LAWLTYRLLERPV-----RFGA 375 >gi|300311314|ref|YP_003775406.1| O-antigen acetylase [Herbaspirillum seropedicae SmR1] Length = 662 Score = 82.4 bits (202), Expect = 5e-17, Method: Compositional matrix adjust. Identities = 62/168 (36%), Positives = 93/168 (55%), Gaps = 18/168 (10%) Query: 1 MTAGALASARPSFDYIPGLDGLRACAVLLVLVAHMGFSTSIPGGF-GVTVFFFISGTLIT 59 ++AGA S P+ Y P +DGLRA AV V++ H F GGF GV +FF ISG LI+ Sbjct: 7 LSAGAAPSLHPA--YRPDIDGLRAVAVGSVVIFH-AFPEWFAGGFIGVDIFFVISGFLIS 63 Query: 60 RLLLAEHAQNGSIAVGRFYVRRLFRLYPALLVAVALG---------ALVFTLAGGIMSWG 110 L+L ++ ++G ++ FYVRR+ R++PAL+ ++L A + G + G Sbjct: 64 -LILFKNLEHGRFSIVDFYVRRVRRIFPALMTVMSLCLVTGWFVLFADEYKQLGKHLFGG 122 Query: 111 KVFASLFYYANYYGIFVHFGQGRDGFDPFSILWSLAVEEQYYLVFPFV 158 F S F + G F + + + P LWSLA+EEQ+YL +P + Sbjct: 123 STFISNFLFWRESGYFDNSAETK----PLLHLWSLAIEEQFYLFWPLL 166 >gi|300311522|ref|YP_003775614.1| O-antigen acetylase [Herbaspirillum seropedicae SmR1] Length = 680 Score = 78.2 bits (191), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 57/162 (35%), Positives = 86/162 (53%), Gaps = 19/162 (11%) Query: 10 RPSF---DYIPGLDGLRACAVLLVLVAHMGFSTSIPGGF-GVTVFFFISGTLITRLLLAE 65 RP+ Y +DGLRA AVL V+ H GF + GGF GV VFF ISG LI+ ++ E Sbjct: 6 RPALSHSKYRSDIDGLRAIAVLAVVSFH-GFPNFVRGGFIGVDVFFVISGYLISTIIF-E 63 Query: 66 HAQNGSIAVGRFYVRRLFRLYPALLVAVALGALV---------FTLAGGIMSWGKVFASL 116 + G+ FY RR+ R++PALL+ ++ +V + G ++ G F S Sbjct: 64 NLDRGTFTFTEFYARRVRRIFPALLLVLSTCFVVGWFVLLTDEYKQLGKHIAGGAGFVSN 123 Query: 117 FYYANYYGIFVHFGQGRDGFDPFSILWSLAVEEQYYLVFPFV 158 F + G F + + + P LWSL +EEQ+Y+++P + Sbjct: 124 FLLRDESGYFDNSAETK----PLLHLWSLGIEEQFYILWPML 161 >gi|300310314|ref|YP_003774406.1| acyltransferase [Herbaspirillum seropedicae SmR1] Length = 656 Score = 75.5 bits (184), Expect = 6e-15, Method: Compositional matrix adjust. Identities = 99/324 (30%), Positives = 144/324 (44%), Gaps = 40/324 (12%) Query: 3 AGALASARPSFDYIPGLDGLRACAVLLVLVAHMGFSTSIPGGF-GVTVFFFISGTLITRL 61 AGA + A Y P +DGLRA AVL V+V H F +PGGF GV +FF ISG LI+ + Sbjct: 10 AGAASPATAHPGYRPDIDGLRAIAVLSVVVFH-AFPEWLPGGFVGVDIFFVISGFLISGI 68 Query: 62 LLAEHAQNGSIAVGRFYVRRLFRLYPALLVAVA---------LGALVFTLAGGIMSWGKV 112 L + Q ++ FY+RR+ R++PALL+ + L + G + G Sbjct: 69 LF-DALQRDRFSIVDFYIRRIRRIFPALLLVMLACLLAGWFILLPEEYRQLGKHLLGGAG 127 Query: 113 FASLFYYANYYGIFVHFGQGRDGFDPFSILWSLAVEEQYYLVFPFVCVALLAGAASLRRF 172 F S N G F + + P LWSL VEEQ+YL +P L+ AA RR Sbjct: 128 FVSNIVLWNEAGYFDTSAETK----PLLHLWSLGVEEQFYLFWP-----LMLWAAWRRRT 178 Query: 173 AW--VLAASVALVLIWRVI-VHHSGGSSDYIYMATDTRIDSILYGAWLALILANDTQKHW 229 VL +AL W ++ +H ++ Y+ M+ + + AW A T Sbjct: 179 GMLAVLLGVMALSFGWNIVWLHRDAVAAFYLPMSRFWELGAGALLAWRHRQGAVPTSVQA 238 Query: 230 RRFSSSHVVQAVCLLLLLVCFTLRHPGFRDTLRYSLQGLALMPLVTAICFTDKLAPLTRL 289 R S V + L+++ + + F AL+P+ A+ P R+ Sbjct: 239 HRLS----VAGLLLIVVAIATITKDDAFPGW-------WALLPVTGAVLLL-AAGPQARI 286 Query: 290 LESKPARRVGNW----SYSLYLYH 309 S + R W SY LYL+H Sbjct: 287 NRSLLSHRTMVWVGLISYPLYLWH 310 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.331 0.143 0.463 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,039,091 Number of extensions: 36940 Number of successful extensions: 151 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 134 Number of HSP's successfully gapped: 6 Length of query: 375 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 288 Effective length of database: 1,207,925 Effective search space: 347882400 Effective search space used: 347882400 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 131 (55.1 bits)