BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0180.0019.p (885 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodies... 367 e-102 gi|300314042|ref|YP_003778134.1| signal transduction protein con... 349 4e-97 gi|300310189|ref|YP_003774281.1| transmembrane protein [Herbaspi... 303 3e-83 gi|300312026|ref|YP_003776118.1| signal transduction protein con... 303 5e-83 gi|300312606|ref|YP_003776698.1| EAL domain transmembrane protei... 294 2e-80 >gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodiesterase with PAS/PAC sensor domains protein [Herbaspirillum seropedicae SmR1] Length = 701 Score = 367 bits (941), Expect = e-102, Method: Compositional matrix adjust. Identities = 194/437 (44%), Positives = 276/437 (63%), Gaps = 4/437 (0%) Query: 431 TVRDISESKTHELALLDQINTDELTGLPNRHWVLRYLNRLLHDAAGDGRGFAVLFADLDD 490 TV I+E K HE + Q D LTGLPNR+ + +L R L A G AV F DLD Sbjct: 248 TVEAITERKAHEAEMRHQATHDALTGLPNRNMLHEHLQRALRVARQKGALTAVAFVDLDQ 307 Query: 491 FKNVNDTLGHSSGDLLLQAVTQRLRELVPAPHEVVRFGGDSFTVIQQGGSIDDA--AQLA 548 FK +ND+LGH GD LL+ V QRL+ + V R GGD F ++ Q + +A A++ Sbjct: 308 FKFINDSLGHQVGDELLKTVAQRLQACLREADMVARQGGDEFVLVLQNQTEGEAGIAEVM 367 Query: 549 DRILQAFSRPLMLSNQNILQVNLSIGISIFPRDGSSVDALLKHADTAMHAAKSEGKKRFR 608 RIL A +RP ++ QV SIG+S +P DG V++LLKHAD+AM+ AK +G+ F+ Sbjct: 368 QRILAAVARPWQAGDRE-FQVTASIGVSRYPADGKDVESLLKHADSAMYRAKEQGRNNFQ 426 Query: 609 FYQPQLSERLQIKIENENALRLAIERDEFVLYFQPRVNAVSGEFCSMEALARWRHPVRGI 668 F+ P + ++ ++E LR A+++DEF LY+QP+++ G EAL RW P +G+ Sbjct: 427 FFAPWMDSQVSNRLEMLINLRRALDQDEFKLYYQPKLSLQDGRLIGAEALIRWESPQQGM 486 Query: 669 VSPLEFIPVAEETGLILQLGELIIRKACAQLAQWRIAGLPLLPMSVNVSSRQLNQGNLND 728 V P FIP AEE GLI+ +GE ++R AC Q W+ AGLP +P++VN+S RQLNQ +L D Sbjct: 487 VPPDRFIPFAEEAGLIVPIGEWVLRTACRQNQAWQQAGLPPIPVAVNLSPRQLNQ-SLPD 545 Query: 729 IIASALAEYAITPAQLELELTESCMMADAIEVAEELASFRKLGIKLLVDDFGTGYSSLSQ 788 +A LA+ + + LELE+TE+ +M DA + L + ++LG+++ VDDFGTGYSSLS Sbjct: 546 FVAGVLAQSGLAASWLELEITENVVMKDAEKSVATLHALKRLGLQISVDDFGTGYSSLSY 605 Query: 789 LQRLDLDVLKVDKAFTAELGVRAESEVLFNAMVSMAHALRMTVVAEGVETEAQLHLLQAL 848 L+R +D LK+DK+F ++ A+S + A++S+AH L + V+AEGVE E Q L+ Sbjct: 606 LRRFPVDALKIDKSFVRDIVRDADSAAIVKAIISLAHILNLRVIAEGVEDEEQHAFLKEN 665 Query: 849 HCDEVQGYYISHPVPAA 865 CDEVQGYY P+ A Sbjct: 666 ACDEVQGYYFGKPMEVA 682 >gi|300314042|ref|YP_003778134.1| signal transduction protein containing a membrane domain, an EAL and a GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 948 Score = 349 bits (896), Expect = 4e-97, Method: Compositional matrix adjust. Identities = 203/533 (38%), Positives = 293/533 (54%), Gaps = 28/533 (5%) Query: 349 DFQVLDCNERGAAIFGINKSELIGSHM----SRFYSPEYFATLMRTFRSAMENG------ 398 D+ + N A I G + +E +G + R PE F T+ R E+ Sbjct: 413 DYTIEYVNPAFARITGYSSTEAVGKSIRFLEGRDSDPEGFETVWIAMREQREHNVVLRNY 472 Query: 399 -----EYEDEFETPPESPLGASWLHRRMVRTRFGLAITVRDISESKTHELALLDQINTDE 453 E ++F P H + DI+ K +E L Q + D Sbjct: 473 HKDGSELWNDFYIAPVRDESGEVTH---------FVAALYDITSMKRYEAELEFQASRDT 523 Query: 454 LTGLPNRHWVLRYLNRLLHDAAGDGRGFAVLFADLDDFKNVNDTLGHSSGDLLLQAVTQR 513 LTGL NRH + +L + + A + V+F DLD FK VNDTLGH +GD LL+ V R Sbjct: 524 LTGLINRHMLRHHLEQAIGVAERQKQALWVVFVDLDRFKFVNDTLGHKAGDTLLRTVAGR 583 Query: 514 LRELVPAPHEVVRFGGDSFTVI--QQGGSIDDAAQLADRILQAFSRPLMLSNQNILQVNL 571 LR V V R GGD F +I +Q D L RI+ A + P+ L + + Sbjct: 584 LRAAVREADTVARLGGDEFIMILPEQDELTLDTRSL-QRIMDAVAAPVTLGGHTLF-MTC 641 Query: 572 SIGISIFPRDGSSVDALLKHADTAMHAAKSEGKKRFRFYQPQLSERLQIKIENENALRLA 631 S+G++ +P DG+ + L+KHAD AM+ K G+ F+FY P ++E+ ++ E LR A Sbjct: 642 SVGVACYPNDGTDGETLIKHADIAMYRGKQTGRNNFQFYTPAMNEQALERLRIEGDLRNA 701 Query: 632 IERDEFVLYFQPRVNAVSGEFCSMEALARWRHPVRGIVSPLEFIPVAEETGLILQLGELI 691 ++ EF+L++QP+V+ SG EAL RW+HP G+VSP FI +AEETGLIL +G + Sbjct: 702 LDHGEFLLHYQPKVDLASGRVIGSEALIRWQHPQLGMVSPARFIGLAEETGLILPIGAWV 761 Query: 692 IRKACAQLAQWRIAGLPLLPMSVNVSSRQLNQGNLNDIIASALAEYAITPAQLELELTES 751 +R AC Q QW++ G + +SVN+S Q Q +L IA L + + P LELELTES Sbjct: 762 LRAACTQCRQWQLGGYADISVSVNLSVGQFLQKDLVSSIADVLEDTGLPPESLELELTES 821 Query: 752 CMMADAIEVAEELASFRKLGIKLLVDDFGTGYSSLSQLQRLDLDVLKVDKAFTAELGVRA 811 MM D LA +KLG++L +DDFGTGYSSL+ L+R +DVLK+D++F ++ + A Sbjct: 822 LMMTDVEHAIGILAGLKKLGVRLSIDDFGTGYSSLAYLKRFPIDVLKIDRSFVRDITIDA 881 Query: 812 ESEVLFNAMVSMAHALRMTVVAEGVETEAQLHLLQALHCDEVQGYYISHPVPA 864 + + +++S+A L++ V+AEGVET+ QL L+ CDE+QGY S PVPA Sbjct: 882 DDAAITASIISLARHLKLRVIAEGVETDEQLDYLRHYGCDEIQGYLFSPPVPA 934 >gi|300310189|ref|YP_003774281.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 706 Score = 303 bits (777), Expect = 3e-83, Method: Compositional matrix adjust. Identities = 173/430 (40%), Positives = 254/430 (59%), Gaps = 4/430 (0%) Query: 445 LLDQINTDELTGLPNRHWVLRYLNRLLHDAAGDGRGFAVLFADLDDFKNVNDTLGHSSGD 504 LL D LTGLPNR + + + A +G+ FAVLF DLD FK +ND+LGH+ GD Sbjct: 258 LLHFATHDVLTGLPNRALLADRIQHAVEVARRNGKTFAVLFMDLDGFKAINDSLGHAVGD 317 Query: 505 LLLQAVTQRLRELVPAPHEVVRFGGDSFTVIQQG-GSIDDAAQLADRILQAFSRPLMLSN 563 LL AV QR+R+ + V R GGD F V+ S D QL++ IL A + + + Sbjct: 318 GLLVAVAQRIRQCLRGEDMVARIGGDEFVVVVGNLSSPDVVEQLSENILAALRQDFQIDD 377 Query: 564 QNILQVNLSIGISIFPRDGSSVDALLKHADTAMHAAKSEGKKRFRFYQPQLSERLQIKIE 623 L+V SIGI+++P G SVDAL+K+AD AM+ AK G+ +RF++P + ++ Sbjct: 378 AT-LRVTSSIGIAVYPNSGDSVDALMKNADAAMYEAKQSGRNTYRFFEPAMHASAMRHLQ 436 Query: 624 NENALRLAIERDEFVLYFQPRVNAVSGEFCSMEALARWRHPVRGIVSPLEFIPVAEETGL 683 AL+ AI+ +F L+FQP+ S E +EAL RW HP G ++P EFIP+AE +G Sbjct: 437 VRQALQQAIDEQQFRLHFQPKYRGASKELTGLEALLRWTHPQLGEMAPTEFIPIAERSGQ 496 Query: 684 ILQLGELIIRKACAQLAQWRIAGLPLLPMSVNVSSRQLNQGNLNDIIASALAEYAITPAQ 743 IL +G+ +++ C Q+A+W AG+ + +++N+S Q+ +L I + I P + Sbjct: 497 ILCIGDWVLQAVCEQIARWDAAGMKPVKVALNLSPLQMRT-DLVARIVELVGAAGIAPQR 555 Query: 744 LELELTESCMMADAIEVAEELASFRKLGIKLLVDDFGTGYSSLSQLQRLDLDVLKVDKAF 803 L E+TE+ M D + + LG + +DDFGTGYSSL+ L + +K+D+ F Sbjct: 556 LMFEITETAAMKDVERSQRVIGELQALGFDIAIDDFGTGYSSLAYLAQFHCRQIKIDRFF 615 Query: 804 TAEL-GVRAESEVLFNAMVSMAHALRMTVVAEGVETEAQLHLLQALHCDEVQGYYISHPV 862 T+ L G + A++++AHAL+M VVAEGVETE QL LQ LHCD+VQG+ + P Sbjct: 616 TSRLDGDDHSGRAIVAAIIALAHALQMEVVAEGVETEGQLRELQLLHCDQVQGFLLGRPA 675 Query: 863 PAADIPPLMQ 872 A +P L+Q Sbjct: 676 EAPSVPGLVQ 685 >gi|300312026|ref|YP_003776118.1| signal transduction protein containing a membrane domain, an EAL and a GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 1106 Score = 303 bits (775), Expect = 5e-83, Method: Compositional matrix adjust. Identities = 163/423 (38%), Positives = 242/423 (57%), Gaps = 6/423 (1%) Query: 452 DELTGLPNRHWVLRYLNRLLHDAAGDGRGFAVLFADLDDFKNVNDTLGHSSGDLLLQAVT 511 DELTGLPNR ++++ + A AV+ DLD FKN+NDT GH +G+ +LQ + Sbjct: 673 DELTGLPNRFLCTQHVSNAITHAEHRNGMVAVILMDLDRFKNINDTFGHETGEAVLQEIA 732 Query: 512 QRLRELVPAPHEVVRFGGDSFTVIQQGGSIDDAAQL---ADRILQAFSRPLMLSNQNILQ 568 QR R + + R GGD F V+ DD QL A ++L +P ++ Q + Sbjct: 733 QRFRNSLRELDILARVGGDEFIVLVD--DFDDPVQLGEIAQKLLTEARKPFIIDGQEAM- 789 Query: 569 VNLSIGISIFPRDGSSVDALLKHADTAMHAAKSEGKKRFRFYQPQLSERLQIKIENENAL 628 +++SIGI+ +P DG + L+K+AD AM+ AK GK +RF+ +++ +I E L Sbjct: 790 LSVSIGIATYPGDGDNAQTLIKNADIAMYRAKHHGKDDYRFFSDEINTNTVERIALEAEL 849 Query: 629 RLAIERDEFVLYFQPRVNAVSGEFCSMEALARWRHPVRGIVSPLEFIPVAEETGLILQLG 688 R AIER EFV+++QP++ G EAL RW+HP+RG++ P EFI +AEET LI Q+G Sbjct: 850 RRAIERAEFVVHYQPKIELAEGRIVGAEALVRWQHPLRGLLPPGEFITLAEETRLIDQIG 909 Query: 689 ELIIRKACAQLAQWRIAGLPLLPMSVNVSSRQLNQGNLNDIIASALAEYAITPAQLELEL 748 L++ AC + R + +S+N++ Q L D I + + P+ LE E+ Sbjct: 910 LLVLDVACRDIGLMRQQQMTCGRISINLTGSQFGDERLLDDIKEVVDRHGTPPSCLEFEI 969 Query: 749 TESCMMADAIEVAEELASFRKLGIKLLVDDFGTGYSSLSQLQRLDLDVLKVDKAFTAELG 808 TES +M + + R G + +DDFGTGYSSL+ L+R ++ LK+DK+F ++ Sbjct: 970 TESMVMHNRERAIAIMDGIRAEGFTISIDDFGTGYSSLAYLKRFPVNTLKIDKSFINDIP 1029 Query: 809 VRAESEVLFNAMVSMAHALRMTVVAEGVETEAQLHLLQALHCDEVQGYYISHPVPAADIP 868 S + A+++M+HAL + VV EGVETE QL L+ DE QGYY S PVP A Sbjct: 1030 DNPNSSAIVQAIIAMSHALNLKVVTEGVETEKQLQALREFGSDEYQGYYFSKPVPYAQFV 1089 Query: 869 PLM 871 L+ Sbjct: 1090 QLL 1092 >gi|300312606|ref|YP_003776698.1| EAL domain transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 695 Score = 294 bits (753), Expect = 2e-80, Method: Compositional matrix adjust. Identities = 166/448 (37%), Positives = 262/448 (58%), Gaps = 10/448 (2%) Query: 437 ESKTHELA---------LLDQINTDELTGLPNRHWVLRYLNRLLHDAAGDGRGFAVLFAD 487 ES+T +LA L + D+LT LPNR + LN+ ++ AA + FA++F D Sbjct: 245 ESRTAKLARSLAAANEELTQMVLHDQLTRLPNRTLLEDRLNQAINKAAREQGHFALMFMD 304 Query: 488 LDDFKNVNDTLGHSSGDLLLQAVTQRLRELVPAPHEVVRFGGDSFTVIQQGGSIDDAAQL 547 LD FK +ND+LGH GD LL V QRL A V R GGD F ++ + +DA + Sbjct: 305 LDGFKAINDSLGHHVGDRLLVEVAQRLTSSSRANDTVARLGGDEFVILVDLNTPEDAMGV 364 Query: 548 ADRILQAFSRPLMLSNQNILQVNLSIGISIFPRDGSSVDALLKHADTAMHAAKSEGKKRF 607 A+++++A ++P + +Q+ L+V+ S+GI+I+P DG++ L+ +AD AM+ K G+ + Sbjct: 365 AEKLVEAVNQPFKV-DQHELRVSASVGIAIYPEDGATRHDLVINADAAMYHTKRSGRNGY 423 Query: 608 RFYQPQLSERLQIKIENENALRLAIERDEFVLYFQPRVNAVSGEFCSMEALARWRHPVRG 667 F++P ++ Q +++ LR+A+ER +F L +QP+ + +G EAL RW HPV G Sbjct: 424 HFFEPSMNANAQQQLQWLQDLRVALERGQFRLVYQPKFSLPAGPVLGAEALLRWEHPVHG 483 Query: 668 IVSPLEFIPVAEETGLILQLGELIIRKACAQLAQWRIAGLPLLPMSVNVSSRQLNQGNLN 727 +V+P +FI +AE +GLIL +G ++ +AC Q+ +W G MSVN+S+ Q +L Sbjct: 484 LVAPDQFIGLAERSGLILPIGAWVLDEACRQMREWFALGYVDWTMSVNLSALQFADPHLL 543 Query: 728 DIIASALAEYAITPAQLELELTESCMMADAIEVAEELASFRKLGIKLLVDDFGTGYSSLS 787 +++ + L + + P L LE+TES M D + L LG+++ +DDFGTGYSSL Sbjct: 544 EVVRTTLERHQLPPPCLTLEVTESTAMHDTEASLQTLRQIADLGVEISIDDFGTGYSSLL 603 Query: 788 QLQRLDLDVLKVDKAFTAELGVRAESEVLFNAMVSMAHALRMTVVAEGVETEAQLHLLQA 847 L+RL + LK+D+ F EL E + +A+V++ L + +VAEGVET Q L A Sbjct: 604 YLKRLPANELKIDRGFVRELRQDGEDAAIVSAIVALGRTLNLRIVAEGVETVEQQDFLTA 663 Query: 848 LHCDEVQGYYISHPVPAADIPPLMQRRY 875 CD +QGY + P+ M+ R+ Sbjct: 664 AGCDALQGYLMGRPMTPELFLQTMRERH 691 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.135 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,684,382 Number of extensions: 112017 Number of successful extensions: 453 Number of sequences better than 1.0e-08: 45 Number of HSP's gapped: 396 Number of HSP's successfully gapped: 48 Length of query: 885 Length of database: 1,619,870 Length adjustment: 94 Effective length of query: 791 Effective length of database: 1,174,780 Effective search space: 929250980 Effective search space used: 929250980 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 135 (56.6 bits)