BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0180.0005.p (270 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312326|ref|YP_003776418.1| methyl-accepting chemotaxis pro... 221 3e-59 >gi|300312326|ref|YP_003776418.1| methyl-accepting chemotaxis protein [Herbaspirillum seropedicae SmR1] Length = 541 Score = 221 bits (564), Expect = 3e-59, Method: Compositional matrix adjust. Identities = 112/188 (59%), Positives = 146/188 (77%), Gaps = 1/188 (0%) Query: 1 MKNWTIKQRILGSFAVILILMVIMAGIAYTRLAKIDDEATSLRQDSTPGLYYSTMINAAW 60 MKNWTIKQRILGSFA+IL+LM +MAGI+ L +I++ AT+LRQDSTPGLYYSTMINAAW Sbjct: 1 MKNWTIKQRILGSFALILLLMALMAGISAVNLGRIENGATALRQDSTPGLYYSTMINAAW 60 Query: 61 YENLLLGQQISQVDGTDATRQSDIELLRANAERLNHLQEEYEKTIFSARDRALFDQFKQA 120 YENLL+ QQI+Q+D +A RQ+D+ LLR N ERL+ LQE+Y+KTIFS DR LFDQFK+ Sbjct: 61 YENLLMSQQIAQIDTNEAERQADLALLRRNGERLDRLQEDYDKTIFSDSDRKLFDQFKET 120 Query: 121 RMAYRQIQQDILRMDLSSPNPAVYKIVSDQLRPAWRTGYALTQKMVEVNKAISNDSAQXA 180 R YR + ++L+MD S P + K+ ++Q+RP WR GY LTQ+MV+ NK+I++D+A Sbjct: 121 RQKYRAMISEVLKMDFSQRAP-IDKMFNEQIRPQWRQGYQLTQRMVDTNKSIADDAASRI 179 Query: 181 RDLCDRAR 188 D A+ Sbjct: 180 NDAVTSAQ 187 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.132 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 721,828 Number of extensions: 25497 Number of successful extensions: 98 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 100 Number of HSP's successfully gapped: 1 Length of query: 270 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 186 Effective length of database: 1,222,130 Effective search space: 227316180 Effective search space used: 227316180 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)