BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0179.0006.p (409 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310376|ref|YP_003774468.1| outer membrane porin [Herbaspir... 340 1e-94 gi|300313813|ref|YP_003777905.1| transmembrane outer membrane po... 141 7e-35 gi|300313580|ref|YP_003777672.1| outer membrane porin [Herbaspir... 133 2e-32 gi|300310676|ref|YP_003774768.1| outer membrane protein (porin) ... 131 9e-32 gi|300313291|ref|YP_003777383.1| porin transmembrane protein [He... 130 2e-31 >gi|300310376|ref|YP_003774468.1| outer membrane porin [Herbaspirillum seropedicae SmR1] Length = 355 Score = 340 bits (872), Expect = 1e-94, Method: Compositional matrix adjust. Identities = 196/361 (54%), Positives = 249/361 (68%), Gaps = 18/361 (4%) Query: 61 MAGACASAQAQTN-VTIYGRIDAGINYQSN--QAGANGSRGSKWGIDGNEWGTSMFGFKG 117 M A AQAQT+ VTIYGR+ AG++ +N + +G+ G++ N+WGTSM G KG Sbjct: 1 MGAFSAQAQAQTSSVTIYGRVVAGVDIMTNVPKTDGSGTTGTRIRAASNQWGTSMIGVKG 60 Query: 118 NEDLGGGLKAIFTLENGFDASTGQVNG----GSGLWTRRSFVGLS-GSAGTLKMGKDLAI 172 EDLGGG A F LE+GF ++TG +NG G+ L+ RRS+VGLS +AG+LK+GK+L I Sbjct: 61 TEDLGGGTNAFFLLESGFGSTTGGLNGTDINGTSLFNRRSYVGLSSANAGSLKLGKNLFI 120 Query: 173 QSDPIWALDPTGQQALSTATLVKERNWPQTNNMVSYESPNFGGFTATVMHGFGEAAGSFT 232 +D +W LDPTGQQ + TA LV+ RNWP +N++ Y +P FGGF ATV GEAAGSF+ Sbjct: 121 NND-VWFLDPTGQQWIGTANLVRGRNWPGASNVIEYTTPTFGGFGATVQTSLGEAAGSFS 179 Query: 233 KGVPTAPGVVSNNGSRDGISLAYVQPTYELRAIYDVQRDTNGQYSTLFASSKELTLGGTV 292 KG G + G +DG+SL Y ELR IYDV RD NG+Y+ ++ +SKEL LGGT Sbjct: 180 KG-----GTSTKYGRKDGLSLVYQNNGLELRGIYDVIRDANGRYTDVYDTSKELILGGTY 234 Query: 293 TIDKLKLFAGYENLRA--NSVATGSPDRANHYWLGANYQITPALTLIGAAYHVNVNAGVG 350 IDKLK+F GYENLRA + A+G PDR NHYWLGANY +TP L+G+ +HVN N G G Sbjct: 235 KIDKLKMFLGYENLRAPDANTASGVPDRVNHYWLGANYDVTPQFQLVGSVFHVNANNGNG 294 Query: 351 SANLFVVGGNYSLSKRTLLYATVGTVRNGANSDFSVEYGG--GGIKGQNQNAFYTGISHS 408 SANLF+ G NY LSKRTLLY GT+RNG N+++S+E GG KG NQ Y GI+HS Sbjct: 295 SANLFMAGVNYFLSKRTLLYVGGGTMRNGNNANYSLEADSSTGGGKGTNQTGGYFGIAHS 354 Query: 409 F 409 F Sbjct: 355 F 355 >gi|300313813|ref|YP_003777905.1| transmembrane outer membrane porin signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 354 Score = 141 bits (356), Expect = 7e-35, Method: Compositional matrix adjust. Identities = 124/359 (34%), Positives = 179/359 (49%), Gaps = 38/359 (10%) Query: 67 SAQAQTNVTIYGRIDAGINYQSNQAGANGSRGSKWGIDGNEWGTSMFGFKGNEDLGGGLK 126 S QAQ++VTIYG +D G+ Y +N AG NG+ GS + TS GF+G EDLGGGLK Sbjct: 18 SVQAQSSVTIYGLVDTGVEYLNN-AGPNGA-GSVTRMTSGATNTSRIGFRGTEDLGGGLK 75 Query: 127 AIFTLENGFDASTGQVNGGSG-LWTRRSFVGLSGSAGTLKMGKDLAIQSDPIWALDPTGQ 185 A+F LENGF G +G + L+ R+S VGL G G + G+ + D + DP G Sbjct: 76 ALFQLENGFKLDNGSFDGDANQLFNRQSTVGLEGGFGRVVAGRSYSTTYDFLGPFDPMGY 135 Query: 186 QA------LSTATLVKERNWPQ-TNNMVSYESPNFGGFTATVMHGFGEAAGSFTKGVPTA 238 A + AT ++ P +N+V Y+ F G M+GFGE AG Sbjct: 136 SANYSWATTAGATGNRKDGMPTGVSNLVKYQG-EFSGVKIGAMYGFGEVAGE-------- 186 Query: 239 PGVVSNNGSRDGISLAYVQPTYELRAIYDVQRD---TNGQYSTLFASSKELTLGGTVTID 295 + NG++ G+ L + + + A YD TN Y A+S L G + + Sbjct: 187 ----TRNGAKYGVGLGWGTGPFSVVATYDRNNSATATNTPYDR--ATSAHLAAGYQPS-E 239 Query: 296 KLKLFAGYENLRANSVATGSPD-RANHYWLGANYQITPALTLIGAAYHVNVNAGVGSAN- 353 K GY + S+ATG+ D R++ +W GA Y+ TPA+TLIGA Y+ N+ A+ Sbjct: 240 AWKFNLGYRYYK-KSLATGAADLRSDFFWGGAAYKFTPAMTLIGAIYYQNIKNVANDADP 298 Query: 354 -LFVVGGNYSLSKRTLLYATV--GTVRNGANSDFSVEYGGGGIKGQNQNAFYTGISHSF 409 ++ + Y++SKRT LY + +NG S + G G +Q G+ H F Sbjct: 299 IMYSLRLKYAMSKRTDLYLSTAYAKAKNGQLVGLSRDDAG---YGSSQFGMTLGMQHRF 354 >gi|300313580|ref|YP_003777672.1| outer membrane porin [Herbaspirillum seropedicae SmR1] Length = 387 Score = 133 bits (335), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 131/398 (32%), Positives = 186/398 (46%), Gaps = 50/398 (12%) Query: 51 IAKPLAIAAVMAGACASAQAQTNVTIYGRIDAGINYQSN----QAGANGSRGSKWGIDGN 106 + K L AV+ +AQAQ++VTIYG +D GI Y S A N + GSK+GI+ Sbjct: 1 MKKSLLALAVLGAFAGAAQAQSSVTIYGIVDTGIAYSSKVGYTDANGNAATGSKFGINSG 60 Query: 107 EWGTSMFGFKGNEDLGGGLKAIFTLENGFDASTGQVNG-----GSGLWTRRSFVGLSGSA 161 S GFKG EDLGGGL A+F LE GF + G + G S L+ R+S VGLSG Sbjct: 61 VIQGSRIGFKGVEDLGGGLSAVFNLETGFTSDDGGLQGSDAVTSSNLFRRKSVVGLSGGF 120 Query: 162 GTLKMGK--DLAIQSDPIWALDPTGQQALSTATLVKERNWPQTNNMVSYESPNFGGFTAT 219 GT+ +G+ D A A+ G ++ + + +TNN VSY + N GFT Sbjct: 121 GTVLLGRQTDFADTISAYTAVADFGGVVANSGSSLNRLQGTRTNNSVSYTTANLSGFTGN 180 Query: 220 VMHGFGEAAGSFTKGVPTAPGVVSNNGSRDGISLAYVQPTYELRAIYDVQRDTNGQYSTL 279 +++GFGE AG + G G +NG G+ + Y Y+ +A + + Sbjct: 181 LIYGFGEQAGKTSAGQAYGIGGKYDNGPL-GLGINY----YQSKAGSTPSDTSLIPAAGA 235 Query: 280 FASSKELTLGGTVTIDKLKLFAGYE----NLRAN------------SVATGS---PDRAN 320 ++ L + G + L + A Y+ + AN +VAT + +A+ Sbjct: 236 TNANTALGVAGNTALKSLNVVASYQFGPARVYANYSRVKQDLKGTPTVATATLANAQKAD 295 Query: 321 HYWLGANYQITPALTLIGAAYH-------VNVNAGVGSANLFVVGGNYSLSKRTLLYATV 373 Y +G Y ++P+L L+ A H VN G +G +Y LSKRT LYA V Sbjct: 296 TYEIGTAYSLSPSLKLLAAVDHSRATFNGVNTK---GKLTQISLGADYFLSKRTDLYAFV 352 Query: 374 GTVRNGANSDFSVEYGGGGIKGQN--QNAFYTGISHSF 409 +R SD GG G + Q A TGI H F Sbjct: 353 SNMR---ASDIQNPGVTGGATGSDASQTAIITGIRHKF 387 >gi|300310676|ref|YP_003774768.1| outer membrane protein (porin) signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 352 Score = 131 bits (330), Expect = 9e-32, Method: Compositional matrix adjust. Identities = 126/381 (33%), Positives = 173/381 (45%), Gaps = 51/381 (13%) Query: 51 IAKPLAIAAVMAGACASAQAQTNVTIYGRIDAGINYQSNQAGANGSRGSKWGIDGNEWGT 110 + K L +AA++AG +A AQ++V+IYG ID G+ Y S+ + G +D Sbjct: 1 MKKTLLVAAMLAGGATTAHAQSSVSIYGLIDLGLTYYSSAPKSGGGGARLLRMDSGIAQG 60 Query: 111 SMFGFKGNEDLGGGLKAIFTLENGFDASTGQVNGGSGLWTRRSFVGLSGSA-GTLKMGKD 169 S GFKG EDLGGG+ A FTLE GF A G + G L+ R+SFVGL + G+L +G+ Sbjct: 61 SRIGFKGTEDLGGGMNAFFTLETGFGADDGSLGQGGLLFGRQSFVGLGSTQWGSLSVGRQ 120 Query: 170 LAIQSD-----PIWALDPTG--------QQALSTATLVKERNWPQTNNMVSYESPNFGGF 216 ++ + AL P G A TA +TNN + Y S FGGF Sbjct: 121 YDFMANIGGQYAMGALSPAGSFGWGLHADAAHGTALNNHIYAGDRTNNSIKYNSAKFGGF 180 Query: 217 TATVMHGFGEAAGSFTKGVPTAPGVVSNNGSRDGISLAYVQPTYELRAIYDVQRDTNGQY 276 + M G GE AG+ G + +NG G +LAY D+ +D+ G + Sbjct: 181 SFGAMVGLGETAGNNNAGRTVSATGQYDNGPFSG-ALAYT----------DI-KDSTGSF 228 Query: 277 ST-LFASSKELTLG-----GTVTIDKLKLFAGYENLRANSVATGSPDRANHYWLGANYQI 330 ST ++ +G G VT K T + +A Y LG Y + Sbjct: 229 STRIYGGGARYQIGAFTPFGVVTQVK---------------NTNTSAKATTYELGTVYAL 273 Query: 331 TPALTLIGAAYHVNVNAGVGSANLFVVGGNYSLSKRTLLYATVGTVRNGANSDFSVEYGG 390 TP L A N GVG+A V +Y +SKRT LY VG V + + G Sbjct: 274 TPLWDLAAAYQFQKRNQGVGNAQALVATVDYKISKRTDLY--VGAVYDRDKGYNAYPVFG 331 Query: 391 GGIKGQ--NQNAFYTGISHSF 409 GGI+ NQ A G+ H F Sbjct: 332 GGIQSATGNQTALRLGMRHRF 352 >gi|300313291|ref|YP_003777383.1| porin transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 365 Score = 130 bits (327), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 110/346 (31%), Positives = 171/346 (49%), Gaps = 11/346 (3%) Query: 71 QTNVTIYGRIDAGINYQSNQAGANGSRGSKWGIDGNEWGTSMFGFKGNEDLGGGLKAIFT 130 Q++VTIYG ID G+ Y +N A ANG+ K + S GFKG EDLGGGL+A+F Sbjct: 24 QSSVTIYGIIDTGVVYTTN-ANANGNSVFKMPSLTGSF-PSRIGFKGTEDLGGGLQAMFV 81 Query: 131 LENGFDASTGQVNGGSGLWTRRSFVGLSGSAGTLKMGKDLAIQSDPIWALDPTGQQALST 190 LE+GF TG + G L+ R+S+VGL S G L +G+ + + D G + Sbjct: 82 LESGFAPDTGAMGQGGRLFGRQSYVGLKNSWGQLMLGRQVNMTYLSQLKTDVMGPNLFAI 141 Query: 191 ATLVKERNWPQTNNMVSYESPNFGGFTATVMHGFGEAAGSFTKGVPT-APGVVSNN--GS 247 ++ +++N + Y NF GFT + FG A T PG V+ N Sbjct: 142 GSIDAYLPNARSDNAIGYLG-NFSGFTVGATYSFGRDAAGPAGPAATNCPGEVAGNSKAC 200 Query: 248 RDGIS-LAYVQPTYELRAIYDVQRDTNGQYSTLFAS---SKELTLGGTVTIDKLKLFAGY 303 R + L Y + + + YD+ G + + +S + +TL G + +K+ G Sbjct: 201 RQVTALLGYDGKGWGVTSSYDILYGNTGASNGMTSSDNTDQRVTLNGYFMLGDVKIGGGV 260 Query: 304 ENLRANSVATGSPDRANHYWLGANYQITPALTLIGAAYHVNVNAGVGSANLFVVGGNYSL 363 + R + A + D ++ Y+LG +Y ++ AL L ++V L V Y+L Sbjct: 261 VDRRTRAAANTNTD-SDLYYLGVSYPLSVALVLDAQVSRLDVKNSPNDVTLSVARLTYNL 319 Query: 364 SKRTLLYATVGTVRNGANSDFSVEYGGGGIKGQNQNAFYTGISHSF 409 SKRT +Y ++G ++NG S +++ GG G+NQ TGI H+F Sbjct: 320 SKRTAVYGSLGYIKNGGTSSVALDAGGTVGAGKNQLGVMTGIRHTF 365 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.313 0.130 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,357,197 Number of extensions: 61546 Number of successful extensions: 225 Number of sequences better than 1.0e-08: 14 Number of HSP's gapped: 178 Number of HSP's successfully gapped: 14 Length of query: 409 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 322 Effective length of database: 1,207,925 Effective search space: 388951850 Effective search space used: 388951850 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 132 (55.5 bits)