BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0178.0055.p (415 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309962|ref|YP_003774054.1| response regulator [Herbaspiril... 555 e-159 gi|300312012|ref|YP_003776104.1| phosphodiesterase transmembrane... 189 3e-49 gi|300310170|ref|YP_003774262.1| diguanylate cyclase/phosphodies... 180 2e-46 gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodies... 174 2e-44 gi|300311036|ref|YP_003775128.1| signal transduction protein [He... 170 2e-43 >gi|300309962|ref|YP_003774054.1| response regulator [Herbaspirillum seropedicae SmR1] Length = 420 Score = 555 bits (1429), Expect = e-159, Method: Compositional matrix adjust. Identities = 280/402 (69%), Positives = 344/402 (85%), Gaps = 1/402 (0%) Query: 10 IETLLIVDDSPLQRLHTVGLMRDLGVEMIYEAGDGQEALDLLALLKLPPSLVIVDLEMPG 69 I++ L+VDDS +QR H+V L++ LG ++IYEAG+G EALDLL LLKLPPSL++VDLEMPG Sbjct: 10 IKSALVVDDSAVQRQHSVQLLQQLGADLIYEAGNGNEALDLLGLLKLPPSLMVVDLEMPG 69 Query: 70 MDGVEFIQHLQERKLNFPIIVASSRETSLLASVETMIEALGMHVLGALQKPLNQEKIMGA 129 MDG+E IQ LQ++ ++ P+IVASSRETSLL SVETMI ALGM+++G LQKPLNQ ++ A Sbjct: 70 MDGIELIQQLQQKGIDVPLIVASSRETSLLLSVETMIHALGMNLIGVLQKPLNQHQLRAA 129 Query: 130 LKSFNSNPAFPAKD-DALPAVCETELPAAIRNGEIIAFYQPKVDIRTGMLKGVEALARWM 188 L+SF + D D P++CE +L AAI G+++ ++QPKVD++TG+LKGVEALARW+ Sbjct: 130 LESFRPHGTEHQPDHDPQPSMCEHDLGAAIEGGQVLPYFQPKVDVQTGILKGVEALARWI 189 Query: 189 HPTNGMVPPDRFIPLAEQCGLIHELTLSMLTQAMAQAALWNERGLPLKVAVNLSPLSLDI 248 HP GMV PDRFIPLAEQCGLIH LTL+M+ QAM QAA+W+ RGL +KVAVNLSPL L+ Sbjct: 190 HPERGMVSPDRFIPLAEQCGLIHPLTLAMMDQAMGQAAIWHSRGLAIKVAVNLSPLLLEK 249 Query: 249 PGFVQKIVDLLAQHALQADQMVLEITEGSVVANLGTALGTLARLRLKGFGLSIDDYGTGF 308 P F+ +I++L+ +HAL A+ +VLEITE SVVA+ G +LG LARLRLKGFGLSIDDYGTGF Sbjct: 250 PDFLHRILELVDKHALPAEHVVLEITESSVVAHKGNSLGMLARLRLKGFGLSIDDYGTGF 309 Query: 309 SSMQQLARIPFTELKIDRSFVHGAHARQNLRVILQSALDMAKRLELVTVAEGIETIEDWR 368 SSMQQLARIPFTELKIDRSFVHGAH R+NLRVILQSALDMA+RLELVTVAEG+ET+EDWR Sbjct: 310 SSMQQLARIPFTELKIDRSFVHGAHDRKNLRVILQSALDMAQRLELVTVAEGVETMEDWR 369 Query: 369 LLQESGCTIGQGYLIARPMPANDLPVWLKGHHRRLGELRPAA 410 LLQ+ GC++GQGYLI +PMPA++LP+WLKGHHRRL ELRP A Sbjct: 370 LLQDFGCSLGQGYLIGKPMPAHELPLWLKGHHRRLHELRPVA 411 >gi|300312012|ref|YP_003776104.1| phosphodiesterase transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 797 Score = 189 bits (481), Expect = 3e-49, Method: Compositional matrix adjust. Identities = 114/319 (35%), Positives = 161/319 (50%), Gaps = 34/319 (10%) Query: 111 MHVLGALQKPLN---QEKIMGALKSFNSNPAFPAKDDALPAVCETELPAAIRNG------ 161 + +L AL++PL+ Q +GA + PA D L + E + A R G Sbjct: 461 LQILRALEQPLSLHQQTVDLGAGIGISGYPAHGQSSDVLLSHAEVAMYIAKRKGSGLVIY 520 Query: 162 -------------------------EIIAFYQPKVDIRTGMLKGVEALARWMHPTNGMVP 196 E + + QPK+++ +G + G EAL RW+HP G + Sbjct: 521 DPAIDQSSQESLSLLSELRRALERDEFMLYLQPKLELCSGQVVGAEALVRWIHPEKGFIG 580 Query: 197 PDRFIPLAEQCGLIHELTLSMLTQAMAQAALWNERGLPLKVAVNLSPLSLDIPGFVQKIV 256 PD FIP AE G I LTL M+ Q + ++G+ LK +VNLS L Sbjct: 581 PDNFIPFAETTGFIRMLTLWMMEQVARLSEQLQQQGVQLKFSVNLSTRDLLDQELPTAFA 640 Query: 257 DLLAQHALQADQMVLEITEGSVVANLGTALGTLARLRLKGFGLSIDDYGTGFSSMQQLAR 316 +L +H LQ + LEITE +++ + A TL RL GF LSIDD+GTG+SS+ L R Sbjct: 641 AILQRHRLQPAALCLEITESAIMDDPARAQNTLERLSNMGFALSIDDFGTGYSSLAYLKR 700 Query: 317 IPFTELKIDRSFVHGAHARQNLRVILQSALDMAKRLELVTVAEGIETIEDWRLLQESGCT 376 +P ELKID+SFV Q+ I++S +D+ L L VAEG+E+ E W LL++ GC Sbjct: 701 LPVDELKIDKSFVMKMAQDQDDAKIVRSTVDLGHNLGLRVVAEGVESEEVWFLLRQMGCD 760 Query: 377 IGQGYLIARPMPANDLPVW 395 GQGY + +PMP W Sbjct: 761 QGQGYFMGKPMPVQHFLAW 779 >gi|300310170|ref|YP_003774262.1| diguanylate cyclase/phosphodiesterase A protein [Herbaspirillum seropedicae SmR1] Length = 1002 Score = 180 bits (457), Expect = 2e-46, Method: Compositional matrix adjust. Identities = 99/254 (38%), Positives = 151/254 (59%), Gaps = 7/254 (2%) Query: 151 ETELPAAIRNGEIIAFYQPKVDIRTGMLKGVEALARWMHPTNGMVPPDRFIPLAEQCGLI 210 E+ L A+R G++ +QP++ + L GVEALARW HP G + P RFIP+AE+CGLI Sbjct: 733 ESALRDALRGGDLELNFQPQIKLHDQSLHGVEALARWHHPQLGQISPGRFIPIAEECGLI 792 Query: 211 HELTLSMLTQAMAQAALWNERGLPL-KVAVNLSPLS---LDIPGFVQKIVDLLAQHALQA 266 EL L +A Q + W RG+ + ++VNLS + LD+P FV LL ++AL+ Sbjct: 793 GELGKWALEEACFQLSDWRRRGIQVPSISVNLSATNFHDLDLPQFV---TGLLRRYALEP 849 Query: 267 DQMVLEITEGSVVANLGTALGTLARLRLKGFGLSIDDYGTGFSSMQQLARIPFTELKIDR 326 + LEITE ++ + T+A + G L++DD+GTG+SS+ L ++P +ELK+D+ Sbjct: 850 SDLTLEITETVLMDTNPSTSQTIAAVHALGIPLAMDDFGTGYSSLGYLRKLPVSELKLDQ 909 Query: 327 SFVHGAHARQNLRVILQSALDMAKRLELVTVAEGIETIEDWRLLQESGCTIGQGYLIARP 386 SFV + + + + + + L+L VAEG+E E + LL GC + QGYL +RP Sbjct: 910 SFVRDMAKDPAVSALTNAVIRIGESLKLTVVAEGVEDAEQYALLISQGCDVAQGYLFSRP 969 Query: 387 MPANDLPVWLKGHH 400 M A+ L W++ H Sbjct: 970 MSADALENWVRDLH 983 >gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodiesterase with PAS/PAC sensor domains protein [Herbaspirillum seropedicae SmR1] Length = 701 Score = 174 bits (440), Expect = 2e-44, Method: Compositional matrix adjust. Identities = 100/246 (40%), Positives = 137/246 (55%), Gaps = 6/246 (2%) Query: 154 LPAAIRNGEIIAFYQPKVDIRTGMLKGVEALARWMHPTNGMVPPDRFIPLAEQCGLIHEL 213 L A+ E +YQPK+ ++ G L G EAL RW P GMVPPDRFIP AE+ GLI + Sbjct: 446 LRRALDQDEFKLYYQPKLSLQDGRLIGAEALIRWESPQQGMVPPDRFIPFAEEAGLIVPI 505 Query: 214 TLSMLTQAMAQAALWNERGLP-LKVAVNLSPLSLD--IPGFVQKIVDLLAQHALQADQMV 270 +L A Q W + GLP + VAVNLSP L+ +P FV + LAQ L A + Sbjct: 506 GEWVLRTACRQNQAWQQAGLPPIPVAVNLSPRQLNQSLPDFVAGV---LAQSGLAASWLE 562 Query: 271 LEITEGSVVANLGTALGTLARLRLKGFGLSIDDYGTGFSSMQQLARIPFTELKIDRSFVH 330 LEITE V+ + ++ TL L+ G +S+DD+GTG+SS+ L R P LKID+SFV Sbjct: 563 LEITENVVMKDAEKSVATLHALKRLGLQISVDDFGTGYSSLSYLRRFPVDALKIDKSFVR 622 Query: 331 GAHARQNLRVILQSALDMAKRLELVTVAEGIETIEDWRLLQESGCTIGQGYLIARPMPAN 390 + I+++ + +A L L +AEG+E E L+E+ C QGY +PM Sbjct: 623 DIVRDADSAAIVKAIISLAHILNLRVIAEGVEDEEQHAFLKENACDEVQGYYFGKPMEVA 682 Query: 391 DLPVWL 396 W+ Sbjct: 683 VFTAWM 688 >gi|300311036|ref|YP_003775128.1| signal transduction protein [Herbaspirillum seropedicae SmR1] Length = 750 Score = 170 bits (431), Expect = 2e-43, Method: Compositional matrix adjust. Identities = 89/244 (36%), Positives = 145/244 (59%), Gaps = 3/244 (1%) Query: 152 TELPAAIRNGEIIAFYQPKVDIRTGMLKGVEALARWMHPTNGMVPPDRFIPLAEQCGLIH 211 +L A++ G+ +QP +++++G++ EAL RW HP GM+ PDRFIPLAE+ GLI Sbjct: 490 NDLYDALQEGQFELHFQPALELQSGVIHKAEALVRWRHPAFGMISPDRFIPLAEESGLIF 549 Query: 212 ELTLSMLTQAMAQAALWNERGL-PLK--VAVNLSPLSLDIPGFVQKIVDLLAQHALQADQ 268 +L +L +A + WN +G P + ++VNLSP+ + ++D L+ Q Sbjct: 550 DLGRWILQEAARVSVRWNAQGTSPFRKQISVNLSPVQFLNGHYDAIVMDQLSLQGCQPGL 609 Query: 269 MVLEITEGSVVANLGTALGTLARLRLKGFGLSIDDYGTGFSSMQQLARIPFTELKIDRSF 328 + +EITEG ++ + L LR G +++DD+GTG+S+M L + LKIDRSF Sbjct: 610 IAIEITEGLLLDDSEAVRSQLNNLRAAGIQVALDDFGTGYSAMSYLKKFSMDYLKIDRSF 669 Query: 329 VHGAHARQNLRVILQSALDMAKRLELVTVAEGIETIEDWRLLQESGCTIGQGYLIARPMP 388 V + + I+++ + MA RL + +AEG+ET+ +LL+++GC QGYL +RP+P Sbjct: 670 VQDMVDADSNKAIVEAIIVMAHRLGMKVIAEGVETMVQAQLLRDAGCEYVQGYLYSRPLP 729 Query: 389 ANDL 392 +D Sbjct: 730 EDDF 733 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.137 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,296,084 Number of extensions: 53124 Number of successful extensions: 276 Number of sequences better than 1.0e-08: 19 Number of HSP's gapped: 262 Number of HSP's successfully gapped: 19 Length of query: 415 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 327 Effective length of database: 1,203,190 Effective search space: 393443130 Effective search space used: 393443130 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)